##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938434_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3218644 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.12138807522671 31.0 31.0 34.0 28.0 34.0 2 31.227960905275637 31.0 31.0 34.0 28.0 34.0 3 31.29211400825938 31.0 31.0 34.0 27.0 34.0 4 34.931723421415974 37.0 35.0 37.0 32.0 37.0 5 34.95593019917705 37.0 35.0 37.0 32.0 37.0 6 34.99254344376079 37.0 35.0 37.0 32.0 37.0 7 34.91451586444478 37.0 35.0 37.0 32.0 37.0 8 34.86796644798244 37.0 35.0 37.0 32.0 37.0 9 36.39264019257799 39.0 37.0 39.0 32.0 39.0 10-11 36.244128272651466 39.0 35.0 39.0 32.0 39.0 12-13 36.108129230818946 39.0 35.0 39.0 31.0 39.0 14-15 37.097976974154335 39.0 36.0 41.0 31.0 41.0 16-17 36.9561989458915 39.0 36.0 41.0 31.0 41.0 18-19 36.962994664834014 39.0 36.0 41.0 31.0 41.0 20-21 37.05264157825469 39.0 36.0 41.0 31.0 41.0 22-23 36.981565528837606 39.0 36.0 41.0 31.0 41.0 24-25 36.86501007877852 39.0 36.0 41.0 30.0 41.0 26-27 36.6772505750869 39.0 36.0 41.0 30.0 41.0 28-29 36.49938467876534 39.0 36.0 41.0 30.0 41.0 30-31 36.274213923627464 39.0 35.0 40.0 29.5 41.0 32-33 35.99338137426817 38.5 35.0 40.0 29.0 41.0 34-35 35.74966880462704 38.0 35.0 40.0 27.0 41.0 36-37 35.455750154412854 38.0 34.5 40.0 26.5 41.0 38-39 35.199166325943466 38.0 34.0 40.0 25.5 41.0 40-41 35.12371731698193 38.0 34.0 40.0 25.5 41.0 42-43 34.93635145732178 38.0 34.0 40.0 25.0 41.0 44-45 34.64632140118634 38.0 33.5 40.0 24.0 41.0 46-47 34.29332367916427 38.0 33.0 40.0 23.5 41.0 48-49 33.93246208651843 38.0 33.0 40.0 22.5 41.0 50-51 33.19755819531455 37.5 32.0 39.5 20.5 40.5 52-53 33.261636888080815 37.0 32.5 39.5 21.0 40.0 54-55 33.3970479493849 37.5 32.5 40.0 19.5 41.0 56-57 33.09628449123295 37.0 32.0 40.0 18.5 41.0 58-59 32.76872341271665 37.0 31.5 40.0 16.0 41.0 60-61 32.32418481198915 36.0 31.0 39.0 13.0 41.0 62-63 31.808880696342932 36.0 31.0 39.0 8.0 40.0 64-65 31.31994218683396 35.0 30.0 39.0 7.5 40.0 66-67 30.7832334051234 35.0 29.0 38.0 4.5 40.0 68-69 30.212850349401798 34.5 28.5 38.0 2.0 40.0 70-71 29.584049059169015 34.0 28.0 37.0 2.0 39.0 72-73 29.021841962018787 34.0 27.5 36.5 2.0 39.0 74-75 28.406026419821515 34.0 26.0 36.0 2.0 39.0 76-77 27.656519484602832 33.0 25.5 35.5 2.0 38.0 78-79 26.861676687449744 32.5 24.5 35.0 2.0 37.0 80-81 26.19525085719328 32.0 22.5 35.0 2.0 36.5 82-83 25.51803709885281 31.5 20.0 35.0 2.0 36.0 84-85 24.840472727024174 31.0 18.0 34.0 2.0 35.5 86-87 24.158638078644298 31.0 12.5 34.0 2.0 35.0 88-89 23.33248659994706 30.0 4.5 34.0 2.0 35.0 90-91 22.47687100530534 29.5 2.0 34.0 2.0 35.0 92-93 21.611140281435286 29.0 2.0 34.0 2.0 35.0 94-95 20.618821621776128 28.5 2.0 34.0 2.0 35.0 96-97 19.34433351436195 26.5 2.0 33.0 2.0 35.0 98-99 17.83445994648678 24.0 2.0 33.0 2.0 34.0 100-101 14.36252238520321 2.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6843325624412273 1101 2 0.5901345185218112 1101 3 0.6815220766622012 1101 4 0.5766938855522739 1101 5 0.5793258406858044 1101 6 0.5033894530083032 1101 7 0.5699208808217477 1101 8 0.6195966477654196 1101 9 0.6372114594504481 1101 10-11 0.6425896190314191 1101 12-13 0.7434656251695984 1101 14-15 0.8701316481642891 1101 16-17 0.8864521941148453 1101 18-19 0.7321290445672233 1101 20-21 0.8281822293634491 1101 22-23 0.8941650047016552 1101 24-25 0.9042375630736856 1101 26-27 0.9083827019782476 1101 28-29 0.9519223501790961 1101 30-31 0.8714377052110223 1101 32-33 0.9368373981229112 1101 34-35 1.0590207364953628 1101 36-37 0.922475909735077 1101 38-39 0.9320453305658916 1101 40-41 1.0198506387176138 1101 42-43 0.9796103223320571 1101 44-45 1.1768428022032893 1101 46-47 1.3411135369519727 1101 48-49 1.2210100417831313 1101 50-51 1.2585005807924503 1101 52-53 1.2207030145532656 1101 54-55 1.1742939084194504 1101 56-57 1.2597793090437506 1101 58-59 1.1425851785135777 1101 60-61 1.1826112599119583 1101 62-63 1.1513903047404561 1101 64-65 1.0588432719508063 1101 66-67 0.9129588394802752 1101 68-69 1.109787677371525 1101 70-71 1.3243116161683481 1101 72-73 1.4049575205082228 1101 74-75 1.3254403787993674 1101 76-77 1.5373587807619948 1101 78-79 1.5042817147623708 1101 80-81 1.6563370305772338 1101 82-83 1.6202397262705226 1101 84-85 1.730123347977358 1101 86-87 1.9433684095863484 1101 88-89 1.9529735313442238 1101 90-91 2.001326655859387 1101 92-93 1.8599325508131521 1101 94-95 1.6586864607509177 1101 96-97 1.8381663218268045 1101 98-99 1.9351614420669208 1101 100-101 1.6964315606475004 1102 1 0.5640450106364945 1102 2 0.617470162332868 1102 3 0.537284625211818 1102 4 0.4430845685660998 1102 5 0.4788588375044611 1102 6 0.4733421032426861 1102 7 0.4149529386213118 1102 8 0.43055919513803786 1102 9 0.6127423170857824 1102 10-11 0.5664232486620264 1102 12-13 0.6007387170997305 1102 14-15 0.655360889988998 1102 16-17 0.6271906579361541 1102 18-19 0.742619723156821 1102 20-21 0.6542571463881259 1102 22-23 0.8023517841157712 1102 24-25 0.5530847780316677 1102 26-27 0.45551861231498236 1102 28-29 0.3773606825661062 1102 30-31 0.38225400079412 1102 32-33 0.5325517698597082 1102 34-35 0.6467309832948587 1102 36-37 0.5987943853284108 1102 38-39 0.6745846975395509 1102 40-41 0.6620990784410523 1102 42-43 0.7626881380152852 1102 44-45 0.8437780227595368 1102 46-47 0.7413832573248982 1102 48-49 0.6247704372777321 1102 50-51 0.6833598750532133 1102 52-53 0.6460281139854658 1102 54-55 0.5678519884130964 1102 56-57 0.601260370871465 1102 58-59 0.7924261637149002 1102 60-61 0.577642156482618 1102 62-63 0.5936768075508958 1102 64-65 0.6704048452993305 1102 66-67 0.5068429358040554 1102 68-69 0.5628644663392421 1102 70-71 0.6303468748612495 1102 72-73 0.7378032427878765 1102 74-75 0.7733931007041654 1102 76-77 0.8639360212428357 1102 78-79 0.7906504847035905 1102 80-81 1.1686702799447843 1102 82-83 1.062375689676994 1102 84-85 1.0102958319805069 1102 86-87 0.9711450051174992 1102 88-89 1.1693575044516358 1102 90-91 1.1925608803544634 1102 92-93 1.1391976999375366 1102 94-95 1.1989039689092067 1102 96-97 1.1074410140459712 1102 98-99 1.2677256845605243 1102 100-101 0.9829946919343584 1103 1 0.5077602215903632 1103 2 0.550622209490065 1103 3 0.4474210057204431 1103 4 0.5240752224409633 1103 5 0.4967128366521081 1103 6 0.5222257896761846 1103 7 0.5165723678127847 1103 8 0.40489341530807366 1103 9 0.6125426763591122 1103 10-11 0.5318080259216629 1103 12-13 0.5606894577248411 1103 14-15 0.7303049391008187 1103 16-17 0.7556985039566726 1103 18-19 0.7565343419065158 1103 20-21 0.7499980709076297 1103 22-23 0.7636611213735378 1103 24-25 0.9044124688402562 1103 26-27 0.8557759759037324 1103 28-29 0.6743472572520801 1103 30-31 0.7986587702180756 1103 32-33 0.9944515318203813 1103 34-35 0.9911295313659352 1103 36-37 0.8493382488553323 1103 38-39 0.8609617621890564 1103 40-41 0.9245688011803779 1103 42-43 0.8681034901972282 1103 44-45 0.8625192085014888 1103 46-47 0.6817013268900425 1103 48-49 0.7806959429950808 1103 50-51 0.8211653106193992 1103 52-53 0.9124035771104602 1103 54-55 0.9759550726073911 1103 56-57 0.9571825475771973 1103 58-59 0.948428096740976 1103 60-61 1.0245452709479324 1103 62-63 1.0374538501469495 1103 64-65 0.8970355086553781 1103 66-67 1.071729421006598 1103 68-69 1.0885804812544144 1103 70-71 1.2246628724415665 1103 72-73 1.203018720312599 1103 74-75 1.2743665116984424 1103 76-77 1.2312169755275093 1103 78-79 1.2854631248307342 1103 80-81 1.1980192162455978 1103 82-83 1.4748880647528289 1103 84-85 1.6277467607997416 1103 86-87 1.675340179570206 1103 88-89 1.6495970713242336 1103 90-91 1.811024058636832 1103 92-93 1.8931523508016959 1103 94-95 1.7200577383550595 1103 96-97 1.7486096510776363 1103 98-99 1.9557971461435812 1103 100-101 1.7386128988887144 1104 1 0.4300670544771066 1104 2 0.47400713172952536 1104 3 0.5171174237054643 1104 4 0.42277138983475027 1104 5 0.4041748024025935 1104 6 0.38916856417891665 1104 7 0.3485270014903463 1104 8 0.2976550525642594 1104 9 0.34570754763382894 1104 10-11 0.4425244145209959 1104 12-13 0.31426810877237443 1104 14-15 0.4662890816953862 1104 16-17 0.5911830572770853 1104 18-19 0.6271451923436757 1104 20-21 0.6232110487885194 1104 22-23 0.6000561913581706 1104 24-25 0.6742396242343673 1104 26-27 0.7019187184659188 1104 28-29 0.7994988620676935 1104 30-31 0.7288063445554229 1104 32-33 0.5081913405744558 1104 34-35 0.5380778609128853 1104 36-37 0.5936797193814911 1104 38-39 0.5999057317749035 1104 40-41 0.5708554873383278 1104 42-43 0.4850939883651151 1104 44-45 0.3920359474870665 1104 46-47 0.5152229475578807 1104 48-49 0.5000617888211067 1104 50-51 0.1880317368554003 1104 52-53 0.328825067146461 1104 54-55 0.5759792679537696 1104 56-57 0.6273418155321693 1104 58-59 0.5576530500226582 1104 60-61 0.6870257555919537 1104 62-63 0.679844663247831 1104 64-65 0.5512907272345657 1104 66-67 0.6803484828893893 1104 68-69 0.5865076090172643 1104 70-71 0.7535878763693518 1104 72-73 0.6207999868953102 1104 74-75 0.5831660741283393 1104 76-77 0.794661953284777 1104 78-79 0.8147437648393989 1104 80-81 0.7539208316904613 1104 82-83 0.8383172353956709 1104 84-85 0.8294066229851218 1104 86-87 0.8228389364515039 1104 88-89 0.9053779211826516 1104 90-91 0.978304358701358 1104 92-93 0.8288271845414705 1104 94-95 0.8495849482342877 1104 96-97 0.8977738175105259 1104 98-99 0.8212189057738968 1104 100-101 0.5583361659342199 1105 1 0.43322376467709134 1105 2 0.4616711930739896 1105 3 0.4578277275484446 1105 4 0.39119659102970417 1105 5 0.40369964059581065 1105 6 0.3711335585523656 1105 7 0.4042044849503341 1105 8 0.36989615359688344 1105 9 0.4332748425174415 1105 10-11 0.49414748991399193 1105 12-13 0.6607186064913861 1105 14-15 0.5839309938599939 1105 16-17 0.63635069230385 1105 18-19 0.7002258942737569 1105 20-21 0.6152464782030194 1105 22-23 0.6775485073036762 1105 24-25 0.6520972138639038 1105 26-27 0.6503175279648943 1105 28-29 0.7633320843537419 1105 30-31 0.6900328072845738 1105 32-33 0.5548793264642597 1105 34-35 0.6766134927162781 1105 36-37 0.7135698868982843 1105 38-39 0.6834890026296563 1105 40-41 0.7287528499770062 1105 42-43 0.7606214397627937 1105 44-45 0.6732100719406944 1105 46-47 0.7635289780975043 1105 48-49 0.6572683400078745 1105 50-51 0.5438539758931356 1105 52-53 0.5722065333322206 1105 54-55 0.5369832188258954 1105 56-57 0.575391579550903 1105 58-59 0.6885703747429872 1105 60-61 0.7895678341652044 1105 62-63 0.6687907990575006 1105 64-65 0.7601650972904679 1105 66-67 0.6843076082170256 1105 68-69 0.5607480711223225 1105 70-71 0.7851058300948637 1105 72-73 0.6703535953755235 1105 74-75 0.5410802213663679 1105 76-77 0.5454932314320189 1105 78-79 0.6138250630400606 1105 80-81 0.6425499611307899 1105 82-83 0.736965472386796 1105 84-85 0.6283859616997276 1105 86-87 0.9584117155406773 1105 88-89 1.0026901133394226 1105 90-91 0.8275081334742893 1105 92-93 0.8165129916790015 1105 94-95 0.8362232016344393 1105 96-97 0.8969708923536075 1105 98-99 0.9403781549922776 1105 100-101 0.7136698896717704 1106 1 0.34901600521170906 1106 2 0.32200632094883375 1106 3 0.10545876761185724 1106 4 0.2946590652951002 1106 5 0.33842677120100007 1106 6 0.007043380089065465 1106 7 0.16839484947345795 1106 8 0.22802636244628616 1106 9 0.2251567440708797 1106 10-11 0.16835081807287366 1106 12-13 0.15604082577775102 1106 14-15 0.4959271735783801 1106 16-17 0.10763376196068464 1106 18-19 -0.0393096167301934 1106 20-21 0.24069304930760183 1106 22-23 0.2736667103400876 1106 24-25 0.3838136708004072 1106 26-27 0.15238606734020976 1106 28-29 0.20264740621522748 1106 30-31 0.24070837509732002 1106 32-33 0.34709219982247674 1106 34-35 0.18601364244489105 1106 36-37 0.15054955243097368 1106 38-39 0.2752513139550885 1106 40-41 0.4255815758525401 1106 42-43 0.04467561730130143 1106 44-45 0.3954512396037231 1106 46-47 0.037710424960359035 1106 48-49 0.45094079214426586 1106 50-51 0.01706191150982761 1106 52-53 0.36856309111534813 1106 54-55 0.22137593717095427 1106 56-57 -0.17175669581764197 1106 58-59 -0.49745848654885805 1106 60-61 0.09838448266626898 1106 62-63 0.21523226727869726 1106 64-65 0.4548508323368736 1106 66-67 -0.07979908443195782 1106 68-69 -0.19297766678654327 1106 70-71 -0.17574522846444296 1106 72-73 0.26131317965841916 1106 74-75 0.373831980127143 1106 76-77 0.32457374909668957 1106 78-79 0.5600905175654987 1106 80-81 0.5080987550447595 1106 82-83 0.346299152518025 1106 84-85 0.3632544262107409 1106 86-87 0.5154961635815454 1106 88-89 0.3332174112883628 1106 90-91 0.5137086046790529 1106 92-93 0.7640694426704044 1106 94-95 0.5644459449709665 1106 96-97 0.49355878635455497 1106 98-99 0.45286797124443723 1106 100-101 0.05271011272337134 1107 1 0.4341327193924265 1107 2 0.4132764253086485 1107 3 0.2837627227911028 1107 4 0.37737913589962346 1107 5 0.43151795041202234 1107 6 0.4075150808924235 1107 7 0.37536620844655744 1107 8 0.25366140393285974 1107 9 0.43578860963712884 1107 10-11 0.47214020607909646 1107 12-13 0.3276814523928877 1107 14-15 0.4930523597315073 1107 16-17 0.5123616326397737 1107 18-19 0.46544374864036797 1107 20-21 0.44881425361943883 1107 22-23 0.5747162878464493 1107 24-25 0.48649289464967893 1107 26-27 0.33746652220614237 1107 28-29 0.3884100512613955 1107 30-31 0.4265914538223612 1107 32-33 0.540663518659926 1107 34-35 0.5564199642016163 1107 36-37 0.5947937938921726 1107 38-39 0.6524636311792662 1107 40-41 0.6392645170612994 1107 42-43 0.5080080844745467 1107 44-45 0.6033851323410246 1107 46-47 0.6511336428983583 1107 48-49 0.550109305193331 1107 50-51 0.5561929212528796 1107 52-53 0.5004200581435185 1107 54-55 0.4201070126423687 1107 56-57 0.46659304109068955 1107 58-59 0.6195962323126807 1107 60-61 0.6789273012723882 1107 62-63 0.6896743960221663 1107 64-65 0.5762993086436552 1107 66-67 0.6082423992895762 1107 68-69 0.5992394838247961 1107 70-71 0.6114752834183506 1107 72-73 0.5299388295458201 1107 74-75 0.6418159408384057 1107 76-77 0.6435883066457215 1107 78-79 0.6348184345823711 1107 80-81 0.5222680914805871 1107 82-83 0.6057955909247781 1107 84-85 0.4859647605342765 1107 86-87 0.4752864191736208 1107 88-89 0.6228263239687344 1107 90-91 0.6258742864896192 1107 92-93 0.6011661168295355 1107 94-95 0.6062219509820466 1107 96-97 0.8029519717063991 1107 98-99 0.642556985910705 1107 100-101 0.23499470619487717 1108 1 0.3288773334560382 1108 2 0.3412298884985283 1108 3 0.2687828106383776 1108 4 0.33380917293327883 1108 5 0.351904724242722 1108 6 0.3360987697248916 1108 7 0.3133783533387273 1108 8 0.26385918364471905 1108 9 0.22563177165428527 1108 10-11 0.29574891221225386 1108 12-13 0.29875283165741706 1108 14-15 0.35502852956783926 1108 16-17 0.3075524942381236 1108 18-19 0.3562901561334826 1108 20-21 0.4316775433003457 1108 22-23 0.3819025134215295 1108 24-25 0.3766788679094475 1108 26-27 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-1.0216264511860693 2316 74-75 -0.9258740395017995 2316 76-77 -0.7855524755514196 2316 78-79 -0.8613323971294164 2316 80-81 -0.7432277219878181 2316 82-83 -0.48845627439635564 2316 84-85 -0.7516942217604559 2316 86-87 -0.8823575420188874 2316 88-89 -0.9410059878637291 2316 90-91 -0.8839671028834033 2316 92-93 -0.89795901108576 2316 94-95 -1.127194467660356 2316 96-97 -1.22584013668979 2316 98-99 -1.2685191817809347 2316 100-101 -0.8817366402034672 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10304.0 3 3424.0 4 3326.0 5 3754.0 6 4571.0 7 5657.0 8 7464.0 9 9709.0 10 11937.0 11 15073.0 12 18957.0 13 22434.0 14 25838.0 15 30603.0 16 34666.0 17 39612.0 18 38297.0 19 36282.0 20 38291.0 21 41377.0 22 46182.0 23 51246.0 24 57600.0 25 64941.0 26 73818.0 27 83742.0 28 96457.0 29 111499.0 30 128389.0 31 150947.0 32 179641.0 33 216370.0 34 264959.0 35 328113.0 36 387544.0 37 386215.0 38 179895.0 39 9510.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92479653983094 18.961924382880806 14.680662389803755 23.432616687484494 2 15.23225496010653 19.98235660545565 40.65158088047875 24.133807553959073 3 19.09883598041483 24.21588447832974 29.676500282239317 27.008779259016112 4 12.69293227211517 16.477625265314902 37.24812958433687 33.58131287823305 5 13.52717272698446 37.685087719843644 34.37568975583188 14.412049797340021 6 32.2643774785272 36.32776311093481 17.35115157860965 14.056707831928343 7 28.685820881374013 32.72072501032635 20.57302892712632 18.020425181173326 8 26.504223368289274 34.852438514234635 19.79954462585787 18.843793491618218 9 26.5789901462598 16.650468070531186 19.956131694431175 36.81441008877784 10-11 24.82392981747259 26.574094939118677 27.63093563752496 20.97103960588377 12-13 26.019843948723874 23.905903641569417 27.689435333579492 22.384817076127213 14-15 22.588648613873293 25.511357508772942 25.969763451407168 25.930230425946593 16-17 22.01451352016187 28.56646705228289 26.973053534436396 22.445965893118842 18-19 22.119235864780546 27.083643823162607 29.167260450135785 21.629859861921062 20-21 22.62794993812672 26.390889940159628 29.33346886084464 21.647691260869014 22-23 22.266496972405932 26.327689481261178 29.27261743554133 22.133196110791555 24-25 22.08423266539873 26.57404032168431 29.354680503620774 21.987046509296185 26-27 21.667478499384988 26.88580140323239 29.11355949221029 22.333160605172335 28-29 21.799877503108657 27.28008256806449 29.039269718355058 21.880770210471788 30-31 21.957591612946143 26.792920516836094 28.86218906184041 22.387298808377356 32-33 21.530353866822335 26.852685940009064 28.896445416752925 22.720514776415676 34-35 22.481380021628112 26.90205709536108 28.650536863998084 21.966026019012723 36-37 22.53563461268874 26.400618929635915 28.692293561384908 22.37145289629043 38-39 21.95877277040637 26.896757583324387 28.657354612257656 22.487115034011588 40-41 22.422040980334604 26.643883412596665 28.820764604307737 22.113311002760998 42-43 22.1608427942903 26.662853694356865 28.566139065657083 22.610164445695755 44-45 22.094154426831455 26.91915013138022 28.801489876872015 22.18520556491631 46-47 22.595670728259027 27.104852346503318 28.024303680415425 22.27517324482223 48-49 22.204788927318436 27.24509127337143 27.98124102204301 22.56887877726712 50-51 22.424360684894136 27.2756889676314 27.988768213435016 22.31118213403945 52-53 22.69036223830154 27.02978947914631 27.977650666443775 22.302197616108373 54-55 22.129420108488365 27.264354744365647 28.22409535417186 22.382129792974123 56-57 22.281137108750475 27.46588706373574 27.8807994244328 22.37217640308098 58-59 22.158700388650463 26.934800684581628 28.092999568718547 22.813499358049366 60-61 21.832147952438934 27.24502917607703 28.30624013358587 22.61658273789817 62-63 22.028104510687747 27.199219517651347 28.432036749781926 22.340639221878984 64-65 21.748505758769117 27.286189471636796 28.219685347510804 22.74561942208328 66-67 21.742374553872835 27.366427499733575 28.06750777450472 22.82369017188887 68-69 22.36625769816288 26.884973083520396 28.19471526293573 22.55405395538099 70-71 22.161922709031117 26.92950845857477 27.89194752631981 23.0166213060743 72-73 22.108896491931347 27.071435452344772 27.966351875848737 22.85331617987514 74-75 22.138820040223642 27.465609159455166 27.729809869956817 22.665760930364375 76-77 22.264455280551278 27.056449570621076 27.92462245820947 22.754472690618172 78-79 22.247137248931566 27.315173706965524 27.762991708857708 22.6746973352452 80-81 22.532493216436155 27.349468267400272 27.476843602228534 22.64119491393504 82-83 22.51234786450649 27.340089853211374 27.56943785271984 22.578124429562294 84-85 22.331654078991033 27.480307547758393 27.62725524873139 22.560783124519183 86-87 22.630721543521854 27.45011039626449 27.23396302393172 22.685205036281936 88-89 22.46555184258309 27.4533109467251 27.1513092824916 22.92982792820021 90-91 22.353087070518455 27.394106699520492 27.383104313805447 22.869701916155606 92-93 22.649403591379297 27.112523959516803 27.469741398813145 22.768331050290747 94-95 22.446054054255647 26.86962005134853 27.65074112255056 23.033584771845266 96-97 22.72683973003519 26.515195427051896 27.57796701538649 23.17999782752642 98-99 22.4726271191181 26.7802860564717 27.19031214127185 23.556774683138354 100-101 23.12859041488029 26.16725082802637 27.558646197300078 23.145512559793264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 906.0 1 603.0 2 428.0 3 584.0 4 531.5 5 522.5 6 888.0 7 1889.0 8 2992.5 9 3289.5 10 2710.5 11 2158.5 12 2136.5 13 2259.5 14 2441.0 15 2661.0 16 2906.0 17 3233.0 18 3590.5 19 4038.0 20 4613.5 21 5489.0 22 6669.0 23 8117.5 24 9978.5 25 12650.0 26 16636.5 27 21444.0 28 25432.0 29 29801.5 30 35770.5 31 44327.0 32 56544.0 33 63193.0 34 66083.5 35 72173.0 36 80696.0 37 89003.0 38 93473.5 39 96600.5 40 99769.5 41 104007.0 42 106741.5 43 109156.5 44 112119.5 45 116736.5 46 119321.0 47 120067.5 48 121313.0 49 119038.0 50 115270.0 51 110525.0 52 106882.0 53 106056.0 54 105364.5 55 99253.5 56 90582.0 57 82437.0 58 76136.0 59 67442.5 60 54811.0 61 45682.5 62 38555.5 63 29115.5 64 20024.5 65 14795.0 66 11518.5 67 8995.0 68 7062.5 69 5469.0 70 4175.5 71 2983.5 72 2134.0 73 1730.0 74 1317.0 75 981.5 76 725.0 77 482.0 78 305.0 79 182.5 80 119.5 81 80.5 82 48.0 83 30.0 84 23.0 85 16.0 86 10.5 87 8.0 88 6.0 89 6.0 90 5.5 91 2.5 92 2.0 93 3.0 94 3.5 95 1.5 96 0.0 97 1.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18184676528376548 2 0.04896471930415417 3 0.04710058024435135 4 0.05319010117304057 5 0.05983886381967064 6 0.06263507240937488 7 0.035573987057903884 8 0.03324381323315036 9 0.039985782832770565 10-11 0.040979990331332074 12-13 0.03852554056925836 14-15 0.033585572060780874 16-17 0.031799105461803166 18-19 0.039814903418955314 20-21 0.04924434016312459 22-23 0.04615297622228492 24-25 0.06637888502114556 26-27 0.051046341254267324 28-29 0.04410242325650181 30-31 0.05238230758045935 32-33 0.04840547758621332 34-35 0.047690890946622246 36-37 0.045888889855479516 38-39 0.026206688282394697 40-41 0.024342549222591874 42-43 0.021189047313092096 44-45 0.003821485072595789 46-47 0.011371248264797225 48-49 0.010718799593866237 50-51 0.008637177643753083 52-53 0.009103212408703789 54-55 0.010144023383760366 56-57 0.00787598752766693 58-59 0.010190626860255436 60-61 0.010547920180050978 62-63 0.003774881596100718 64-65 0.006353607295494624 66-67 0.0034486572606352243 68-69 0.008155608386637354 70-71 0.02255608262361417 72-73 0.019697736065249837 74-75 0.019759874033909934 76-77 0.012147972873048401 78-79 0.004629278665177012 80-81 0.006307003818999553 82-83 0.005359399796933118 84-85 0.004722485618167153 86-87 0.007394418270551201 88-89 0.029406793668389546 90-91 0.036040021822854595 92-93 0.0222453927803137 94-95 0.031845708938298245 96-97 0.034595314051507405 98-99 0.02106477137577191 100-101 0.12183702205027956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3218644.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.68457667797704 #Duplication Level Percentage of deduplicated Percentage of total 1 71.1913140107168 34.65918985760683 2 14.92120612947326 14.52865207876484 3 5.688871864725945 8.308809555283094 4 2.7783339869935126 5.410480561072613 5 1.5508949056520585 3.77523309768508 6 0.9273180923331459 2.708765326264106 7 0.609139967149476 2.0759005006816356 8 0.4443908476748532 1.7307984238894083 9 0.3034832195183738 1.329745686400945 >10 1.42870028525317 12.356952130364029 >50 0.09596760218786728 3.2131591003258833 >100 0.05371775804062168 4.901046381623292 >500 0.003202562635863723 1.0697356019062874 >1k 0.0032666138870672424 3.1977937968395107 >5k 1.2810250537518598E-4 0.41171022145191133 >10k+ 6.405125268759299E-5 0.32202767984048236 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10327 0.320849401176396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7555 0.2347261766135056 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5648 0.17547762349610582 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4632 0.14391153541677798 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4297 0.13350342566621223 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4258 0.1322917352773404 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3947 0.12262928115069577 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3556 0.11048130827764736 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3556 0.11048130827764736 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3384 0.10513744297287926 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.1068984330047065E-5 0.0 12-13 3.1068984330047065E-5 0.0 0.0 3.1068984330047065E-5 0.0 14-15 3.1068984330047065E-5 0.0 0.0 4.66034764950706E-5 0.0 16-17 3.1068984330047065E-5 0.0 0.0 6.213796866009413E-5 0.0 18-19 3.1068984330047065E-5 0.0 0.0 7.767246082511766E-5 0.0 20-21 3.1068984330047065E-5 0.0 0.0 1.7087941381525884E-4 0.0 22-23 3.1068984330047065E-5 0.0 0.0 3.7282781196056476E-4 0.0 24-25 3.1068984330047065E-5 0.0 0.0 8.077935925812237E-4 0.0 26-27 3.1068984330047065E-5 0.0 0.0 0.0016155871851624474 0.0 28-29 3.1068984330047065E-5 0.0 0.0 0.004303054329711518 0.0 30-31 3.1068984330047065E-5 0.0 0.0 0.014229594823161554 0.0 32-33 3.1068984330047065E-5 0.0 0.0 0.03430015870037196 0.0 34-35 3.1068984330047065E-5 0.0 0.0 0.06397103873556689 0.0 36-37 3.1068984330047065E-5 0.0 0.0 0.11074539464445275 0.0 38-39 3.1068984330047065E-5 0.0 0.0 0.18113217864417439 0.0 40-41 3.1068984330047065E-5 0.0 0.0 0.26104160634105544 0.0 42-43 3.1068984330047065E-5 0.0 0.0 0.3463570373113647 0.0 44-45 3.1068984330047065E-5 0.0 0.0 0.45575093113746035 0.0 46-47 3.1068984330047065E-5 0.0 0.0 0.5838794225145745 0.0 48-49 3.1068984330047065E-5 0.0 0.0 0.7171964342748064 0.0 50-51 3.1068984330047065E-5 0.0 0.0 0.8611235041837494 0.0 52-53 3.1068984330047065E-5 0.0 0.0 1.0059205056539338 0.0 54-55 3.1068984330047065E-5 0.0 0.0 1.163952894448718 0.0 56-57 3.1068984330047065E-5 0.0 0.0 1.3475084538706361 0.0 58-59 3.1068984330047065E-5 0.0 0.0 1.5438799693286986 0.0 60-61 3.1068984330047065E-5 0.0 0.0 1.7572928226917919 0.0 62-63 3.1068984330047065E-5 0.0 0.0 1.9639015684866048 0.0 64-65 3.1068984330047065E-5 0.0 0.0 2.1889342219891357 0.0 66-67 3.1068984330047065E-5 0.0 0.0 2.4541390722304177 0.0 68-69 3.1068984330047065E-5 0.0 0.0 2.738451347834678 0.0 70-71 3.1068984330047065E-5 0.0 0.0 3.044636188407292 0.0 72-73 3.1068984330047065E-5 0.0 0.0 3.3606077590438703 0.0 74-75 3.1068984330047065E-5 0.0 0.0 3.6844087137316213 0.0 76-77 3.1068984330047065E-5 0.0 0.0 4.032148320845673 0.0 78-79 3.1068984330047065E-5 0.0 0.0 4.417496933491247 0.0 80-81 3.1068984330047065E-5 0.0 0.0 4.820352918806802 0.0 82-83 3.1068984330047065E-5 0.0 0.0 5.246060142097107 0.0 84-85 3.1068984330047065E-5 0.0 0.0 5.6870067022013 0.0 86-87 3.1068984330047065E-5 3.1068984330047065E-5 0.0 6.137506974986982 0.0 88-89 3.1068984330047065E-5 3.1068984330047065E-5 0.0 6.6027494808372715 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6590 0.0 45.067352 1 GTATCAA 11205 0.0 39.12106 1 TATCAAC 14235 0.0 30.646843 2 ATCAACG 14230 0.0 30.490631 3 TCAACGC 14405 0.0 30.054234 4 CAACGCA 14790 0.0 29.270979 5 AACGCAG 15475 0.0 28.15386 6 ACGCAGA 17335 0.0 24.910662 7 CGCAGAG 17430 0.0 24.720764 8 TACATGG 12105 0.0 23.830023 2 GTACATG 12505 0.0 23.673885 1 TAAGGTG 1875 0.0 23.570496 5 ACATGGG 12110 0.0 22.878365 3 GAGTACT 10150 0.0 22.112122 12-13 GCAGAGT 19110 0.0 21.900826 9 GTGTAAG 1790 0.0 21.803371 1 GTACTTT 10685 0.0 20.672834 14-15 AGAGTAC 16395 0.0 20.572104 10-11 GTAAGGT 2020 0.0 20.23241 4 AGTACTT 10725 0.0 19.930117 12-13 >>END_MODULE