FastQCFastQC Report
Thu 26 May 2016
SRR938434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938434_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3218644
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105560.32796419858797676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83690.26001632985816386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62510.19421222104712418No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA45180.14036967120315264No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45060.13999684339119206No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC41900.13017904434289718No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39850.12380990255523754No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38460.11949131373336101No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG36980.11489310405251404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64200.046.8176841
GTATCAA111050.040.4707371
ATCAACG144800.030.696483
TCAACGC146250.030.392144
TATCAAC146850.030.312592
CAACGCA148750.029.8813465
AACGCAG154450.028.8444486
ACGCAGA175800.025.179347
CGCAGAG176600.025.011498
TACATGG124100.023.5680962
GTACATG129050.023.3277911
TAAGGTG22450.023.2928095
GTACCGT2303.6379788E-922.714976
ACATGGG126950.022.3556443
GTAAGGT22850.021.8448264
GCAGAGT201050.021.7808159
AGGTAAG20700.021.5962662
ACCGTAT1353.8007295E-421.1088628
AGAGTAC173200.020.82702410-11
GAGTACT111650.020.80163412-13
Produced by FastQC (version 0.11.5)