FastQCFastQC Report
Thu 26 May 2016
SRR938433_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938433_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221289
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102900.31943734324986056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75060.23301231277293033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60230.18697484143769777No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45330.14072006578732923No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA43560.13522537096174855No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42530.13202789318189084No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA39010.12110059047791116No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35600.11051476598343087No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34360.10666537525816529No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG33270.10328163663676249No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64100.043.6531451
GTATCAA112450.040.6107981
TATCAAC144000.031.7495212
ATCAACG143900.031.6725043
TCAACGC144000.031.6175064
CAACGCA146200.031.0767145
AACGCAG152650.029.8523736
ACGCAGA172050.026.4529137
CGCAGAG173000.026.1154258
TACATGG120300.026.0737132
GTACATG124600.025.862121
ACATGGG122500.024.3639743
GTGTAGC19250.023.27731
GCAGAGT187000.023.2689139
TAAGGTG18850.022.9432335
GAGTACT103700.021.48255512-13
AGAGTAC164000.020.83957910-11
AGTACTT109600.020.43445412-13
GTACTTT109200.020.03129214-15
GTAAGGT19150.019.8538994
Produced by FastQC (version 0.11.5)