##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938433_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3221289 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.160597822797023 31.0 31.0 34.0 28.0 34.0 2 31.311224481876664 31.0 31.0 34.0 28.0 34.0 3 31.371005830274775 31.0 31.0 34.0 28.0 34.0 4 35.0444784680915 37.0 35.0 37.0 32.0 37.0 5 35.05307657897196 37.0 35.0 37.0 32.0 37.0 6 35.06239396713551 37.0 35.0 37.0 32.0 37.0 7 34.975717794957234 37.0 35.0 37.0 32.0 37.0 8 34.93152896247434 37.0 35.0 37.0 32.0 37.0 9 36.43332343046526 39.0 37.0 39.0 32.0 39.0 10-11 36.294832596516486 39.0 35.0 39.0 32.0 39.0 12-13 36.13178684060946 39.0 35.0 39.0 31.0 39.0 14-15 37.12626839752658 39.0 36.0 41.0 31.0 41.0 16-17 36.95526604412085 39.0 36.0 41.0 31.0 41.0 18-19 36.83174778791968 39.0 36.0 41.0 30.5 41.0 20-21 36.81362538412418 39.0 36.0 40.5 30.5 41.0 22-23 36.91448392242981 39.0 36.0 41.0 30.5 41.0 24-25 36.83172015922818 39.0 36.0 41.0 30.0 41.0 26-27 36.638231931379025 39.0 36.0 41.0 30.0 41.0 28-29 36.45166438031484 39.0 36.0 40.5 30.0 41.0 30-31 36.17876213528187 39.0 35.0 40.0 29.0 41.0 32-33 35.86818351287326 38.0 35.0 40.0 28.0 41.0 34-35 35.61218149008052 38.0 34.5 40.0 27.0 41.0 36-37 35.31130395316906 38.0 34.0 40.0 26.5 41.0 38-39 34.98155971103493 38.0 34.0 40.0 25.0 41.0 40-41 34.85929700812314 38.0 34.0 40.0 25.0 41.0 42-43 34.61812740179475 38.0 33.0 40.0 24.0 41.0 44-45 34.30647545128674 38.0 33.0 40.0 24.0 41.0 46-47 33.917750006286305 38.0 33.0 40.0 22.5 41.0 48-49 33.523878484668714 38.0 32.5 40.0 20.5 41.0 50-51 32.738970331441855 36.5 31.5 39.5 18.5 40.5 52-53 32.76223694924609 37.0 31.5 39.0 18.0 40.0 54-55 32.820197132265996 37.0 31.5 40.0 16.5 41.0 56-57 32.482910878222974 37.0 31.0 39.5 13.5 41.0 58-59 32.02750777716622 36.0 31.0 39.0 8.5 41.0 60-61 31.52461716412281 36.0 30.0 39.0 7.5 40.5 62-63 30.930028631395693 35.0 29.0 39.0 4.0 40.0 64-65 30.416577494288774 35.0 28.0 38.0 2.0 40.0 66-67 29.842459804134307 34.5 28.0 38.0 2.0 40.0 68-69 29.18991108838729 34.0 26.5 37.5 2.0 39.5 70-71 28.516582647505395 34.0 26.0 37.0 2.0 39.0 72-73 27.847926715051024 33.5 25.5 36.0 2.0 39.0 74-75 27.12479802339995 33.0 24.5 35.5 2.0 38.5 76-77 26.348344560205557 32.0 21.5 35.0 2.0 37.5 78-79 25.51085435054104 31.5 19.0 35.0 2.0 37.0 80-81 24.713132382720083 31.0 15.0 35.0 2.0 36.0 82-83 23.93642932999802 31.0 7.5 34.0 2.0 36.0 84-85 23.162308628626615 30.0 2.0 34.0 2.0 35.0 86-87 22.402613363780773 29.5 2.0 34.0 2.0 35.0 88-89 21.577419474005595 29.0 2.0 34.0 2.0 35.0 90-91 20.71907037834854 28.5 2.0 34.0 2.0 35.0 92-93 19.829952388624555 26.5 2.0 34.0 2.0 35.0 94-95 18.900071834597888 25.5 2.0 33.0 2.0 35.0 96-97 17.811301159256434 23.5 2.0 33.0 2.0 35.0 98-99 16.538855098067884 10.0 2.0 32.0 2.0 34.5 100-101 13.769457971638062 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7068404849719983 1101 2 0.6045745852693329 1101 3 0.6658134989489319 1101 4 0.5763976843638972 1101 5 0.6478352423261313 1101 6 0.5830962595756688 1101 7 0.5604045900692682 1101 8 0.560951658192387 1101 9 0.694071626818122 1101 10-11 0.7153466732313234 1101 12-13 0.8346924882807301 1101 14-15 0.9663809178654574 1101 16-17 1.1173955210544477 1101 18-19 1.1756214099940294 1101 20-21 1.185333049109218 1101 22-23 1.03936952308716 1101 24-25 1.1885598316566117 1101 26-27 1.2080146363657818 1101 28-29 1.2826454067274469 1101 30-31 1.2063919260121807 1101 32-33 1.2495859462102885 1101 34-35 1.3791346705857919 1101 36-37 1.3876924799164456 1101 38-39 1.4724109022822347 1101 40-41 1.7767476213008493 1101 42-43 1.8145399250385665 1101 44-45 1.7997506634200349 1101 46-47 1.9922775657463063 1101 48-49 1.9781416300881816 1101 50-51 1.955248099739464 1101 52-53 2.0062177829644696 1101 54-55 2.1487382588636947 1101 56-57 2.1930819323902355 1101 58-59 2.4877557234657033 1101 60-61 2.4672978469461704 1101 62-63 2.3832615425525496 1101 64-65 2.560039212049702 1101 66-67 2.6371923971226714 1101 68-69 2.9453204774430723 1101 70-71 3.027518927728835 1101 72-73 3.2980226878533045 1101 74-75 3.4978566076827207 1101 76-77 3.8296146814547 1101 78-79 4.256900458065015 1101 80-81 4.640214261275677 1101 82-83 4.790077586712492 1101 84-85 4.9012825097321695 1101 86-87 5.218502113068098 1101 88-89 5.628800730092085 1101 90-91 5.814642520882 1101 92-93 5.869040627543299 1101 94-95 6.163947277222572 1101 96-97 6.33208530889268 1101 98-99 6.71034122935108 1101 100-101 6.485011986546185 1102 1 0.6445164919549349 1102 2 0.614854667331592 1102 3 0.5391394415818027 1102 4 0.47052080323948786 1102 5 0.4482965239755359 1102 6 0.5362876404898245 1102 7 0.5005055113583055 1102 8 0.5968398802192212 1102 9 0.7525843120634761 1102 10-11 0.6898290056344507 1102 12-13 0.7946211935064653 1102 14-15 1.069673283781583 1102 16-17 1.0580628503356309 1102 18-19 1.0745752436013234 1102 20-21 1.0642437490595213 1102 22-23 0.8877432504212877 1102 24-25 0.7874021676276612 1102 26-27 0.9066002092087757 1102 28-29 0.9225548706468061 1102 30-31 0.9495431040643538 1102 32-33 1.0110533188162734 1102 34-35 1.1663920835857766 1102 36-37 1.2580936802681464 1102 38-39 1.3312497977547935 1102 40-41 1.5803375266234312 1102 42-43 1.639344963457468 1102 44-45 1.724982578503436 1102 46-47 1.7603334929606547 1102 48-49 1.6454435147168027 1102 50-51 1.8390640585554223 1102 52-53 1.8966538342916053 1102 54-55 2.110216462668639 1102 56-57 2.234509994115321 1102 58-59 2.405296850165243 1102 60-61 2.3590578530626125 1102 62-63 2.748950852796309 1102 64-65 2.721399865489559 1102 66-67 2.8089057653706924 1102 68-69 3.166757331553189 1102 70-71 3.2393838690492203 1102 72-73 3.258976534203189 1102 74-75 3.4396529301721124 1102 76-77 3.5992032233204725 1102 78-79 4.00686446025674 1102 80-81 4.526208246774612 1102 82-83 4.741613068445993 1102 84-85 4.935787523940153 1102 86-87 5.305199489369436 1102 88-89 5.579926537653538 1102 90-91 5.989991667765796 1102 92-93 5.944663740047602 1102 94-95 6.244160906693626 1102 96-97 6.084758385080612 1102 98-99 6.431576750586601 1102 100-101 6.288594883445915 1103 1 0.569318729000134 1103 2 0.6025730880617175 1103 3 0.522966423985185 1103 4 0.5256527870332874 1103 5 0.5191888787970242 1103 6 0.5224247922391214 1103 7 0.5297757315491225 1103 8 0.42504780523195507 1103 9 0.653250898114436 1103 10-11 0.6370427150278459 1103 12-13 0.6726010228861625 1103 14-15 0.8563268137520978 1103 16-17 0.9984207637242335 1103 18-19 1.110582982709353 1103 20-21 1.0737415925893146 1103 22-23 0.901530419657675 1103 24-25 1.0343870179817003 1103 26-27 1.0496268282714638 1103 28-29 1.1894781130920364 1103 30-31 1.2160834218938135 1103 32-33 1.2203044701348134 1103 34-35 1.3008357761598148 1103 36-37 1.3894247616545812 1103 38-39 1.4478407752284 1103 40-41 1.5534899000842515 1103 42-43 1.7999519651353353 1103 44-45 1.7910278629527028 1103 46-47 1.8943510521205766 1103 48-49 2.0061254218238957 1103 50-51 2.108775464497448 1103 52-53 2.189125080337668 1103 54-55 2.3262580624100124 1103 56-57 2.432740967892009 1103 58-59 2.605119729717636 1103 60-61 2.914356157649113 1103 62-63 2.966335996786956 1103 64-65 3.026453472900016 1103 66-67 3.215317034958172 1103 68-69 3.4209695023265745 1103 70-71 3.560857481451336 1103 72-73 3.695115609736444 1103 74-75 3.856553097388634 1103 76-77 4.164081361276747 1103 78-79 4.22932213395611 1103 80-81 4.603688085722343 1103 82-83 4.945249060835543 1103 84-85 5.134047812519963 1103 86-87 5.230862950543813 1103 88-89 5.44997656010786 1103 90-91 5.870864807140993 1103 92-93 5.957769441898954 1103 94-95 6.092936881339579 1103 96-97 6.339458168924651 1103 98-99 6.561084664972718 1103 100-101 6.202060994515632 1104 1 0.5727789771678182 1104 2 0.5050735757685061 1104 3 0.5285880801425051 1104 4 0.44153726629514267 1104 5 0.473642489309249 1104 6 0.43651390997512607 1104 7 0.4824559685077858 1104 8 0.5488988852482208 1104 9 0.7260104545037009 1104 10-11 0.710467722384621 1104 12-13 0.833295759508033 1104 14-15 0.8235131068957458 1104 16-17 0.9931283944030156 1104 18-19 1.1388315215746942 1104 20-21 1.0098790768901935 1104 22-23 0.9695081248224042 1104 24-25 1.1149485268641612 1104 26-27 1.1371137250866568 1104 28-29 1.0877740549260082 1104 30-31 1.1807841539673731 1104 32-33 1.3185292949236072 1104 34-35 1.3564450531855954 1104 36-37 1.4346048625202883 1104 38-39 1.512799928937497 1104 40-41 1.6744447670105274 1104 42-43 1.6969986608080418 1104 44-45 1.6591038553222006 1104 46-47 1.749790111244586 1104 48-49 1.8346701396445937 1104 50-51 1.890734933787229 1104 52-53 1.975552805872752 1104 54-55 2.1636970603164087 1104 56-57 2.421456585532219 1104 58-59 2.4862460195253178 1104 60-61 2.5580120561503072 1104 62-63 2.7046054620026645 1104 64-65 2.809274153914984 1104 66-67 2.919497636219763 1104 68-69 3.2863526945655828 1104 70-71 3.4167639544972808 1104 72-73 3.396014094464878 1104 74-75 3.4223753516575464 1104 76-77 3.6346375291160733 1104 78-79 3.89897653340066 1104 80-81 4.226004753980796 1104 82-83 4.497069819922338 1104 84-85 4.746647395271548 1104 86-87 4.863937936503472 1104 88-89 5.325455331253362 1104 90-91 5.68230191388805 1104 92-93 5.6721937260742195 1104 94-95 5.868214767658081 1104 96-97 6.123729087477521 1104 98-99 6.336397567379247 1104 100-101 6.265172819530894 1105 1 0.5642079220473306 1105 2 0.504657267640674 1105 3 0.514186865076617 1105 4 0.41162972731495273 1105 5 0.2806408412459902 1105 6 0.386007057137185 1105 7 0.41142946343390463 1105 8 0.34674879684505555 1105 9 0.4359107971686811 1105 10-11 0.5285403320605795 1105 12-13 0.5481521535116443 1105 14-15 0.7294753777485781 1105 16-17 0.8456466247297811 1105 18-19 0.8162034302099173 1105 20-21 0.8413681159864197 1105 22-23 0.7780736783986768 1105 24-25 0.7738970931488183 1105 26-27 0.9152030775493571 1105 28-29 1.03382882926023 1105 30-31 0.8817197949461999 1105 32-33 0.9614407675079164 1105 34-35 1.0841661068014687 1105 36-37 1.125524739174935 1105 38-39 1.1730067426923227 1105 40-41 1.300081113830089 1105 42-43 1.5035286430240191 1105 44-45 1.5164129521994099 1105 46-47 1.5315268675230413 1105 48-49 1.5768468699217877 1105 50-51 1.484823016833218 1105 52-53 1.7061335086341103 1105 54-55 1.8274203854423519 1105 56-57 1.9859908991988746 1105 58-59 1.9437169134948604 1105 60-61 2.286511449041985 1105 62-63 2.561501850755807 1105 64-65 2.665361280283051 1105 66-67 2.8008409582404674 1105 68-69 2.844318985976468 1105 70-71 3.1072101075794087 1105 72-73 3.251745881999671 1105 74-75 3.4610901152360682 1105 76-77 3.5603989899126773 1105 78-79 3.9400783763995086 1105 80-81 4.227003339640856 1105 82-83 4.606098564752411 1105 84-85 4.643294656379453 1105 86-87 4.949277964243809 1105 88-89 5.4367400784435596 1105 90-91 5.464081454826644 1105 92-93 5.5283849294742495 1105 94-95 5.7496102435795 1105 96-97 6.024705583883701 1105 98-99 6.084896550424336 1105 100-101 5.732272523573661 1106 1 0.2702918133824319 1106 2 0.30207510455203135 1106 3 0.23164650365408335 1106 4 0.2194739652361335 1106 5 0.30877153662446233 1106 6 0.18278509133509857 1106 7 0.21804707672595924 1106 8 0.24574796390991338 1106 9 0.21773275547278814 1106 10-11 0.16790801632215846 1106 12-13 0.1407621755170041 1106 14-15 0.560952030495109 1106 16-17 0.3171673377009938 1106 18-19 0.3710695835739273 1106 20-21 0.4582162571624693 1106 22-23 0.3187666434011973 1106 24-25 0.3492014142095172 1106 26-27 0.5492076018161711 1106 28-29 0.4069065452158753 1106 30-31 0.6004127494992133 1106 32-33 0.6838400405160385 1106 34-35 0.5138208984058963 1106 36-37 0.6627179925011504 1106 38-39 0.6998425250431737 1106 40-41 0.7024103715223688 1106 42-43 0.28096827355903997 1106 44-45 0.5669068606451049 1106 46-47 0.9220844427550787 1106 48-49 1.047133579450346 1106 50-51 0.5445457586023394 1106 52-53 0.9684270473691612 1106 54-55 0.7950536094623075 1106 56-57 0.8240426706045199 1106 58-59 0.26492248696479237 1106 60-61 1.5332142880886188 1106 62-63 1.8992887640037885 1106 64-65 1.9536874564728066 1106 66-67 1.8421099290096414 1106 68-69 1.5645366967238594 1106 70-71 2.009579742505963 1106 72-73 2.329494881895453 1106 74-75 2.6104060900557045 1106 76-77 2.773262223466425 1106 78-79 3.152040523911065 1106 80-81 3.4787079601438897 1106 82-83 3.710376874166318 1106 84-85 3.8571769453295737 1106 86-87 4.238631216279423 1106 88-89 4.106836075432636 1106 90-91 4.529968484916527 1106 92-93 5.026447555527071 1106 94-95 4.969050245713401 1106 96-97 5.104579682075819 1106 98-99 5.3090946498436296 1106 100-101 4.906746952684939 1107 1 0.3850255156316962 1107 2 0.2664479613854347 1107 3 0.30035933225815015 1107 4 0.25623847381981335 1107 5 0.21877439936282173 1107 6 0.27751463186292824 1107 7 0.3292786779456165 1107 8 0.16285605085257515 1107 9 0.308632428310311 1107 10-11 0.359983588705461 1107 12-13 0.4638143805985422 1107 14-15 0.5211614777449327 1107 16-17 0.5556864863286393 1107 18-19 0.6474920277980587 1107 20-21 0.702655152447548 1107 22-23 0.6883536734016573 1107 24-25 0.7113504333351344 1107 26-27 0.7911080158381552 1107 28-29 0.8030216173467508 1107 30-31 0.9395283197216813 1107 32-33 0.9886128792951823 1107 34-35 1.0074186532797995 1107 36-37 1.0487473317487996 1107 38-39 1.0898723678910969 1107 40-41 1.1421753622046893 1107 42-43 1.374621266311209 1107 44-45 1.395276411202289 1107 46-47 1.2563838244142573 1107 48-49 1.5030892125144604 1107 50-51 1.466528052983179 1107 52-53 1.6909923825752173 1107 54-55 1.6199258568754047 1107 56-57 1.7060534253314614 1107 58-59 1.9322340842182015 1107 60-61 2.132538292673974 1107 62-63 2.385346286386369 1107 64-65 2.4019501243565955 1107 66-67 2.543936759460724 1107 68-69 2.612843185295386 1107 70-71 2.6212438642201477 1107 72-73 2.916324570642402 1107 74-75 2.982714631766836 1107 76-77 3.2441287073751184 1107 78-79 3.389365887541924 1107 80-81 3.6698841511167934 1107 82-83 3.9741165078459844 1107 84-85 4.24434062910343 1107 86-87 4.588141005654027 1107 88-89 4.727565179885982 1107 90-91 5.080202390720533 1107 92-93 5.166139191870805 1107 94-95 5.167233526361802 1107 96-97 5.33942733766014 1107 98-99 5.192486173827991 1107 100-101 4.799908704154825 1108 1 0.2762802432839031 1108 2 0.39197961836958584 1108 3 0.29978518048840996 1108 4 0.3089881525764042 1108 5 0.3201279827635162 1108 6 0.3651024801742295 1108 7 0.25600324511691497 1108 8 0.30348752164947257 1108 9 0.4139211147046282 1108 10-11 0.4206988506347358 1108 12-13 0.49312015830977174 1108 14-15 0.40815462226292 1108 16-17 0.6438582730875737 1108 18-19 0.7028696871131572 1108 20-21 0.6297339286801318 1108 22-23 0.5844516047383266 1108 24-25 0.6671654713040098 1108 26-27 0.7184182384181099 1108 28-29 0.7212231040976533 1108 30-31 0.7727203185459146 1108 32-33 0.775650991022637 1108 34-35 1.0399512017101102 1108 36-37 1.011304188913428 1108 38-39 1.1108018653503393 1108 40-41 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0.3134076539575119 2311 38-39 0.41221470916134706 2311 40-41 0.24131838437084951 2311 42-43 0.5876285588230843 2311 44-45 0.6993510177772322 2311 46-47 0.6761614588674831 2311 48-49 0.75398022341124 2311 50-51 0.9643103308837055 2311 52-53 1.1024403389740058 2311 54-55 1.100836292010861 2311 56-57 1.2281465902755784 2311 58-59 1.2311654745354446 2311 60-61 1.4090896128735473 2311 62-63 1.5512003553612708 2311 64-65 1.595798671276384 2311 66-67 1.6467613193145745 2311 68-69 1.489876683852355 2311 70-71 1.773061494603816 2311 72-73 1.8991218671718677 2311 74-75 1.8991862990114754 2311 76-77 2.221413167296973 2311 78-79 2.225174083534821 2311 80-81 2.4279988283759195 2311 82-83 2.564790736260882 2311 84-85 2.8318521124337614 2311 86-87 3.1696876101667044 2311 88-89 3.2667333719367093 2311 90-91 3.48868902647893 2311 92-93 3.632170868012146 2311 94-95 3.6661933851141164 2311 96-97 3.6177552323519038 2311 98-99 4.0413994455827975 2311 100-101 3.7740922668139607 2312 1 -0.378438691409805 2312 2 -0.3873745243173943 2312 3 -0.26417557504379374 2312 4 -0.25258833710514494 2312 5 -0.2752957909137095 2312 6 -0.15823952929331853 2312 7 -0.2552179107473691 2312 8 -0.24013812655400812 2312 9 -0.311103583535683 2312 10-11 -0.36896070801874714 2312 12-13 -0.3148189389712144 2312 14-15 -0.22706122498364323 2312 16-17 -0.24684883853510087 2312 18-19 -0.15154605077935202 2312 20-21 -0.14616229325778107 2312 22-23 -0.2271726772079532 2312 24-25 -0.23620475231031435 2312 26-27 -0.09967721643725014 2312 28-29 -0.1201303059974137 2312 30-31 -0.11775652125575675 2312 32-33 -0.08927403193769834 2312 34-35 -0.20722922177613867 2312 36-37 -0.13346877443424887 2312 38-39 0.1106355950586817 2312 40-41 0.12905250195957052 2312 42-43 0.25129101482331606 2312 44-45 0.05865029058411153 2312 46-47 0.1143906217618138 2312 48-49 0.37016085760820516 2312 50-51 0.4264653921171657 2312 52-53 0.4879167207763331 2312 54-55 0.583448043835368 2312 56-57 0.6427172628174702 2312 58-59 0.7525593369328547 2312 60-61 0.8915189465305282 2312 62-63 0.958282380813845 2312 64-65 1.028277196257676 2312 66-67 1.21005703853562 2312 68-69 1.4056618870106483 2312 70-71 1.5437212390862491 2312 72-73 1.5262148842060448 2312 74-75 1.667549891004228 2312 76-77 1.6845170232247142 2312 78-79 1.7957325579985586 2312 80-81 1.926667701841577 2312 82-83 2.328050249166452 2312 84-85 2.441550478508571 2312 86-87 2.711640974735836 2312 88-89 2.681384956091474 2312 90-91 2.9445606321470486 2312 92-93 2.8761830824031236 2312 94-95 3.0371996792545843 2312 96-97 2.9760303859942496 2312 98-99 2.8554493067117157 2312 100-101 2.5893347993735443 2313 1 -0.44054533133283513 2313 2 -0.4542506269253934 2313 3 -0.4095796370130351 2313 4 -0.36642736381558194 2313 5 -0.2836381390484064 2313 6 -0.23688089271916368 2313 7 -0.26710076664958393 2313 8 -0.23896538060770922 2313 9 -0.350436961573223 2313 10-11 -0.2533637950417358 2313 12-13 -0.3246301573614758 2313 14-15 -0.31822601292297037 2313 16-17 -0.3278143278703496 2313 18-19 -0.20094157424430392 2313 20-21 -0.13730391554073407 2313 22-23 -0.25875340824671156 2313 24-25 -0.2146212058923851 2313 26-27 -0.21651656133648345 2313 28-29 -0.1072284159625454 2313 30-31 0.0023034169012348116 2313 32-33 0.1107591510777226 2313 34-35 0.15019384671433045 2313 36-37 0.15719065047895242 2313 38-39 0.3229855277887026 2313 40-41 0.2943068360492518 2313 42-43 0.45224445538759284 2313 44-45 0.37623130628351475 2313 46-47 0.41912833952638096 2313 48-49 0.5688257548427558 2313 50-51 0.6967389983236814 2313 52-53 0.673087561534345 2313 54-55 0.6813996541566567 2313 56-57 0.9525496170957126 2313 58-59 1.071362733401024 2313 60-61 1.1503368110563947 2313 62-63 1.222300890000728 2313 64-65 1.3465239266870128 2313 66-67 1.4037257060444368 2313 68-69 1.6288297457348229 2313 70-71 1.8016347472853624 2313 72-73 1.8528193709310337 2313 74-75 1.8726718759763799 2313 76-77 2.093529038796241 2313 78-79 2.3284811454605396 2313 80-81 2.4353934050207364 2313 82-83 2.546930324861197 2313 84-85 2.7915788623954647 2313 86-87 2.836425260482521 2313 88-89 2.7691565382819725 2313 90-91 2.9388084985892746 2313 92-93 3.00922832010626 2313 94-95 3.16131726414903 2313 96-97 3.2504697015070363 2313 98-99 3.294334294939059 2313 100-101 3.137235155988238 2314 1 -0.435454839513401 2314 2 -0.37418077529666505 2314 3 -0.5025725013277444 2314 4 -0.40565348910246257 2314 5 -0.3813530571710544 2314 6 -0.3808934413619198 2314 7 -0.46293825747468276 2314 8 -0.38549660511127115 2314 9 -0.4661866402404087 2314 10-11 -0.42063539375210723 2314 12-13 -0.47981160113591415 2314 14-15 -0.5100157936619851 2314 16-17 -0.5204885410900033 2314 18-19 -0.2906788042874524 2314 20-21 -0.3048452929337131 2314 22-23 -0.3572927393751897 2314 24-25 -0.4234675986313263 2314 26-27 -0.3623618666395245 2314 28-29 -0.3377035700008548 2314 30-31 -0.26643709769430046 2314 32-33 -0.07869864443868124 2314 34-35 -0.35274146399088124 2314 36-37 -0.3194876435706675 2314 38-39 -0.04767382218679472 2314 40-41 0.04806905198969247 2314 42-43 0.21432338499277392 2314 44-45 0.07955046974797142 2314 46-47 0.21099105813917873 2314 48-49 0.1993684833124334 2314 50-51 0.22416740398810475 2314 52-53 0.1961956772466209 2314 54-55 0.2047099082099848 2314 56-57 0.4045829755655461 2314 58-59 0.6437090219606532 2314 60-61 0.696853304630519 2314 62-63 0.6769096071023704 2314 64-65 0.6860554963128607 2314 66-67 0.9468306877267558 2314 68-69 1.295679024846141 2314 70-71 1.457990092894967 2314 72-73 1.403410638966637 2314 74-75 1.4284610282616441 2314 76-77 1.6362334100512541 2314 78-79 1.8488397760344526 2314 80-81 1.9435627270033464 2314 82-83 2.2109851351234475 2314 84-85 2.4308707166281387 2314 86-87 2.6865815129670274 2314 88-89 2.8005258795103636 2314 90-91 2.9113352739896463 2314 92-93 2.924049562996302 2314 94-95 2.8902345894439563 2314 96-97 3.0048766427737164 2314 98-99 2.9984507246462044 2314 100-101 2.885926282424018 2315 1 -0.7386151813591901 2315 2 -0.6245904685079822 2315 3 -0.6619184488766088 2315 4 -0.5565548637145454 2315 5 -0.3904419911313539 2315 6 -0.5355725404059797 2315 7 -0.5646088148333419 2315 8 -0.5214602493754299 2315 9 -0.7223789843594162 2315 10-11 -0.6257118983078414 2315 12-13 -0.6084490760929171 2315 14-15 -0.7500127762389681 2315 16-17 -0.5819432659954202 2315 18-19 -0.6405613898671945 2315 20-21 -0.6208246040149135 2315 22-23 -0.6122921113647308 2315 24-25 -0.7358038297312461 2315 26-27 -0.8002695934473039 2315 28-29 -0.8856113537878727 2315 30-31 -0.7105626234827369 2315 32-33 -0.7089916144128452 2315 34-35 -0.8279011111852554 2315 36-37 -0.5564566743560633 2315 38-39 -0.5872612725807755 2315 40-41 -0.5492164564275086 2315 42-43 -0.5244165857298029 2315 44-45 -0.5580370832086672 2315 46-47 -0.3450811093045161 2315 48-49 -0.27124719237355777 2315 50-51 -0.2040626445408691 2315 52-53 -0.07028191739653522 2315 54-55 -0.15580550133334015 2315 56-57 0.11376892289640494 2315 58-59 0.34040573651218864 2315 60-61 0.4289221197122828 2315 62-63 0.4574366750962078 2315 64-65 0.6059775982427951 2315 66-67 0.6424509898802988 2315 68-69 0.8968378837449507 2315 70-71 1.0270360644989829 2315 72-73 1.2081663998787207 2315 74-75 1.4357536293570874 2315 76-77 1.5758247498985583 2315 78-79 1.850968322714209 2315 80-81 2.0508368222285576 2315 82-83 2.1896687013408958 2315 84-85 2.315232067155332 2315 86-87 2.5515816851434643 2315 88-89 2.8107769424830877 2315 90-91 2.7322382118151225 2315 92-93 2.665083815702257 2315 94-95 2.648451926644942 2315 96-97 2.724118065600152 2315 98-99 2.832727560077984 2315 100-101 2.6954814305366295 2316 1 -0.961562114123673 2316 2 -0.9326901302132065 2316 3 -0.8945304920881298 2316 4 -0.6062537735350872 2316 5 -0.702728127049113 2316 6 -0.634042029839847 2316 7 -0.594386083533287 2316 8 -0.7265934432140995 2316 9 -0.6922726327934683 2316 10-11 -0.7047111389056937 2316 12-13 -0.903614129728858 2316 14-15 -1.3037179646096675 2316 16-17 -1.0471000048272217 2316 18-19 -0.9083472393211593 2316 20-21 -0.8652558054400359 2316 22-23 -0.9295396918616561 2316 24-25 -1.1402040099785111 2316 26-27 -0.8508247982162302 2316 28-29 -0.9041578310658949 2316 30-31 -0.829946550425305 2316 32-33 -0.7078235250605687 2316 34-35 -0.8484331812394856 2316 36-37 -0.7742422270677665 2316 38-39 -0.7431240266190287 2316 40-41 -0.849162027876126 2316 42-43 -0.7126127635002604 2316 44-45 -0.6122488837280287 2316 46-47 -0.49933139170423146 2316 48-49 -0.4341278148545271 2316 50-51 -0.26257191808056035 2316 52-53 -0.22420053656276195 2316 54-55 -0.1562794288272542 2316 56-57 -0.09352683392150851 2316 58-59 0.01324408311247538 2316 60-61 -0.1466346526298956 2316 62-63 0.11678572063117798 2316 64-65 0.25330264739234565 2316 66-67 0.5429419994069278 2316 68-69 0.579958994754854 2316 70-71 0.29347504814040093 2316 72-73 0.6070980073246623 2316 74-75 0.9121061526253342 2316 76-77 0.8516039757212219 2316 78-79 1.1713654997577798 2316 80-81 1.6497108702772358 2316 82-83 2.1218971987301245 2316 84-85 2.0835451716978 2316 86-87 2.311807745977692 2316 88-89 2.344974552701551 2316 90-91 2.467506270280399 2316 92-93 2.5639464843303443 2316 94-95 2.6717547092874305 2316 96-97 2.612317537639761 2316 98-99 2.5249833439931955 2316 100-101 2.332835552686584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9689.0 3 3161.0 4 3476.0 5 3969.0 6 4681.0 7 6103.0 8 7814.0 9 10057.0 10 12797.0 11 16568.0 12 20402.0 13 24802.0 14 28584.0 15 33777.0 16 38959.0 17 44712.0 18 43758.0 19 43159.0 20 45688.0 21 50391.0 22 56144.0 23 62883.0 24 70908.0 25 79479.0 26 89207.0 27 100268.0 28 112302.0 29 124598.0 30 140972.0 31 159429.0 32 180939.0 33 207590.0 34 242696.0 35 285809.0 36 328043.0 37 333168.0 38 181217.0 39 13089.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8869061705207 18.95203778388356 14.718040758168696 23.443015287427045 2 15.203452190784716 19.932079090346736 40.675811808897286 24.188656909971257 3 19.091857424888996 24.238905515867852 29.689773388269135 26.979463670974013 4 12.669006055723589 16.454058459052977 37.27675361785928 33.60018186736416 5 13.520196601834694 37.72148611803937 34.35817092208671 14.400146358039226 6 32.288591612993 36.35440273139313 17.33025703721374 14.026748618400129 7 28.757150269863395 32.65488382723153 20.621811471049732 17.966154431855337 8 26.513887478522317 34.88512991261731 19.770026920663074 18.830955688197296 9 26.60391304347826 16.673509316770186 19.985155279503104 36.737422360248445 10-11 24.83783002844913 26.554388638731773 27.649298926081485 20.958482406737613 12-13 26.02179377476433 23.915252676709734 27.67088328898093 22.392070259545005 14-15 22.57710473686801 25.48876951479913 25.992929157900306 25.941196590432547 16-17 22.01069482519376 28.539897404943083 27.023327474218494 22.426080295644663 18-19 22.09755056972449 27.088322200500293 29.192123344669035 21.62200388510618 20-21 22.631909108836176 26.383638472539023 29.37289506741219 21.611557351212614 22-23 22.26543207288817 26.304380870224346 29.28862425766548 22.141562799222005 24-25 22.06865449544336 26.553141675468105 29.360486650152417 22.017717178936117 26-27 21.6733837468916 26.89272123583924 29.100506506255936 22.333388511013226 28-29 21.832925614547758 27.26291689601657 29.033205725505233 21.870951763930435 30-31 21.97178785943524 26.807278631027824 28.839489357367704 22.38144415216923 32-33 21.569085636555346 26.796038058069005 28.924946033578657 22.709930271796992 34-35 22.458325544247494 26.904672147284835 28.666672049157878 21.970330259309794 36-37 22.5399629482188 26.351759649082496 28.702988922349626 22.40528848034908 38-39 21.956466053239897 26.868635904250777 28.67227827447717 22.502619768032154 40-41 22.444769939757492 26.652803360726168 28.819825924692598 22.082600774823742 42-43 22.177099349460615 26.6587015541905 28.584906070458953 22.57929302588993 44-45 22.089654367350185 26.93691881258941 28.757569096725483 22.215857723334924 46-47 22.630640532738436 27.13050741022769 27.968534961541952 22.27031709549192 48-49 22.182421760630703 27.249736011890253 27.97752351985802 22.59031870762102 50-51 22.460325529736743 27.319568185293186 27.9318670964427 22.28823918852737 52-53 22.72370891774647 27.002622825520557 28.007234695618283 22.26643356111469 54-55 22.098674805977073 27.299717128642282 28.222561066947982 22.379046998432667 56-57 22.290686999960574 27.484701754451322 27.907923980502442 22.316687265085662 58-59 22.191654428251947 26.947817241674244 28.056702214339413 22.803826115734395 60-61 21.864444612778726 27.264766986722567 28.28825148147964 22.58253691901907 62-63 21.989623230923858 27.241944809869185 28.458393967969393 22.310037991237568 64-65 21.77178610607519 27.27208236256361 28.240236458781077 22.71589507258012 66-67 21.801404042625748 27.34114336635883 28.059121472954864 22.79833111806056 68-69 22.4389239842189 26.880199362447488 28.155863210587295 22.52501344274632 70-71 22.19392079560816 26.96148135821182 27.85724531355076 22.98735253262926 72-73 22.12629724589256 27.106408160299942 27.916455284330716 22.850839309476783 74-75 22.179986771043268 27.4996511138201 27.674039872218398 22.64632224291823 76-77 22.32538739199958 27.063256394880526 27.869560162241818 22.741796050878076 78-79 22.303311198448846 27.308100734395513 27.743885638517092 22.644702428638553 80-81 22.59811899145555 27.30197532596635 27.459533231213047 22.640372451365057 82-83 22.570708445893604 27.351610606709308 27.555422189118644 22.522258758278436 84-85 22.46882133025369 27.41822996582745 27.578110629658177 22.534838074260687 86-87 22.756325582189497 27.394470150516337 27.251249128830622 22.597955138463544 88-89 22.555946482888213 27.45924436366809 27.150024862688564 22.83478429075513 90-91 22.461184612057274 27.388779887743915 27.365739303866953 22.78429619633186 92-93 22.798539964006725 27.108993056089183 27.434851257242006 22.657615722662086 94-95 22.51672038303184 26.835749017269965 27.69386018841093 22.953670411287266 96-97 22.817941329888967 26.566652798132097 27.56580826647458 23.04959760550436 98-99 22.621358801297657 26.76321989963622 27.18622501966076 23.429196279405367 100-101 23.1849345292996 26.209722529528317 27.630916540084176 22.974426401087904 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 913.0 1 611.5 2 437.5 3 614.5 4 560.0 5 518.0 6 905.0 7 2023.0 8 3102.5 9 3324.0 10 2761.0 11 2205.0 12 2225.0 13 2421.5 14 2606.0 15 2731.0 16 2991.0 17 3312.5 18 3633.0 19 4089.0 20 4682.0 21 5623.0 22 6923.0 23 8520.0 24 10400.0 25 13055.0 26 17139.0 27 21817.0 28 25668.0 29 30467.0 30 36521.5 31 44722.5 32 56390.0 33 62834.5 34 65904.0 35 72053.5 36 80687.0 37 88824.5 38 92965.5 39 95691.5 40 99088.5 41 103651.0 42 106429.0 43 108705.0 44 111711.5 45 116225.5 46 118777.0 47 119673.0 48 120657.5 49 118245.5 50 114071.0 51 109964.5 52 106974.0 53 105798.5 54 104841.5 55 98825.5 56 90299.0 57 82397.5 58 76134.0 59 67374.0 60 55196.0 61 46309.0 62 39100.5 63 29824.0 64 21215.5 65 15914.5 66 12229.5 67 9522.5 68 7429.0 69 5788.0 70 4460.0 71 3181.0 72 2329.5 73 1811.5 74 1382.0 75 1063.0 76 788.5 77 552.5 78 349.5 79 211.5 80 126.0 81 80.0 82 48.0 83 34.5 84 23.5 85 11.0 86 8.0 87 9.5 88 7.0 89 4.5 90 4.5 91 4.0 92 3.0 93 2.5 94 2.0 95 1.0 96 0.5 97 0.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3030153457203002 2 0.03330964716298351 3 0.03998399398501656 4 0.04221912408355786 5 0.05547468730685139 6 0.05690268709203055 7 0.029677560752853903 8 0.033713212319664586 9 0.040015037458607405 10-11 0.03040708238223891 12-13 0.032828473322325316 14-15 0.027333778496744624 16-17 0.02067495341150701 18-19 0.02436912676881832 20-21 0.0364450379956595 22-23 0.02975516943683103 24-25 0.05108203579374592 26-27 0.043088341344101694 28-29 0.03528090773600258 30-31 0.03571551636627449 32-33 0.03102795185405594 34-35 0.030143212856716676 36-37 0.02890147391308262 38-39 0.018129388577057197 40-41 0.017275693053308786 42-43 0.017912084261921236 44-45 0.009219911656482855 46-47 0.017741345157171556 48-49 0.015521736795425683 50-51 0.00718656413628209 52-53 0.004485781933878022 54-55 0.006627781611646766 56-57 0.005215303563263029 58-59 0.0066898685588284695 60-61 0.005743042614307502 62-63 0.004703086249013982 64-65 0.008148911817598484 66-67 0.0028094343599720487 68-69 0.006860607663578152 70-71 0.012634693751476503 72-73 0.013255563223293533 74-75 0.010073607180231267 76-77 0.009142302972505727 78-79 0.006348390349329104 80-81 0.007791911871303693 82-83 0.013100345855339274 84-85 0.0042374341451512105 86-87 0.00136591283799746 88-89 0.018610562417715394 90-91 0.027457952391108032 92-93 0.012712302435453633 94-95 0.020768083832279564 96-97 0.017663736473194427 98-99 0.012603650277885655 100-101 0.10433711473885143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3221289.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.42444693012373 #Duplication Level Percentage of deduplicated Percentage of total 1 70.9536480807352 34.35891165984238 2 14.884912439376091 14.415873049602121 3 5.885007739211874 8.549347348524984 4 2.7965439282627313 5.4168437216767344 5 1.5843582574502486 3.836083617810144 6 0.9564777254301234 2.779014291296188 7 0.5958804509819351 2.019862689269104 8 0.4206557939560531 1.6296019336219165 9 0.3054061264333803 1.3310210485447092 >10 1.4608115633549532 12.532440667721382 >50 0.0950017396737804 3.17508261846292 >100 0.05451969271821818 4.91536920106357 >500 0.0033559623399691354 1.1174480262017643 >1k 0.0032268868635871475 3.178707729983403 >5k 1.290754745434859E-4 0.42280888075099754 >10k+ 6.453773727174295E-5 0.32158351562774523 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10290 0.31943734324986056 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7506 0.23301231277293033 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6023 0.18697484143769777 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4533 0.14072006578732923 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4356 0.13522537096174855 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4253 0.13202789318189084 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3901 0.12110059047791116 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3560 0.11051476598343087 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3436 0.10666537525816529 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3327 0.10328163663676249 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.1043473590851364E-5 0.0 8 0.0 0.0 0.0 3.1043473590851364E-5 0.0 9 0.0 0.0 0.0 6.208694718170273E-5 0.0 10-11 0.0 0.0 0.0 7.760868397712841E-5 0.0 12-13 0.0 0.0 0.0 1.2417389436340546E-4 0.0 14-15 0.0 0.0 0.0 1.2417389436340546E-4 0.0 16-17 0.0 0.0 0.0 1.2417389436340546E-4 0.0 18-19 0.0 0.0 0.0 1.862608415451082E-4 0.0 20-21 0.0 0.0 0.0 3.41478209499365E-4 0.0 22-23 0.0 0.0 0.0 7.605651029758585E-4 0.0 24-25 0.0 0.0 0.0 0.0014745649955654398 0.0 26-27 0.0 0.0 0.0 0.0021264779409733187 0.0 28-29 0.0 0.0 0.0 0.004827260143377387 0.0 30-31 0.0 0.0 0.0 0.014248954378200777 0.0 32-33 0.0 0.0 0.0 0.03506360342086662 0.0 34-35 0.0 0.0 0.0 0.06509816412001532 0.0 36-37 0.0 0.0 0.0 0.11208246139976885 0.0 38-39 0.0 0.0 0.0 0.18666440670178924 0.0 40-41 0.0 0.0 0.0 0.26495604709791637 0.0 42-43 0.0 0.0 0.0 0.34635203485312865 0.0 44-45 0.0 0.0 0.0 0.4534830622151567 0.0 46-47 0.0 0.0 0.0 0.5799541736242853 1.5521736795425682E-5 48-49 0.0 0.0 0.0 0.71376706653765 3.1043473590851364E-5 50-51 0.0 0.0 0.0 0.8577466970520187 3.1043473590851364E-5 52-53 0.0 0.0 0.0 0.9969301108966007 3.1043473590851364E-5 54-55 0.0 0.0 0.0 1.1527373048490839 3.1043473590851364E-5 56-57 0.0 0.0 0.0 1.3362197555078108 3.1043473590851364E-5 58-59 0.0 1.5521736795425682E-5 0.0 1.530256987187427 3.1043473590851364E-5 60-61 0.0 3.1043473590851364E-5 0.0 1.7407006946598087 3.1043473590851364E-5 62-63 0.0 3.1043473590851364E-5 0.0 1.9510667934482129 3.1043473590851364E-5 64-65 0.0 3.1043473590851364E-5 0.0 2.1727482383604824 3.1043473590851364E-5 66-67 0.0 3.1043473590851364E-5 0.0 2.430564286532503 3.1043473590851364E-5 68-69 0.0 3.1043473590851364E-5 0.0 2.712873635367705 3.1043473590851364E-5 70-71 0.0 3.1043473590851364E-5 0.0 3.0123810685722394 3.1043473590851364E-5 72-73 0.0 3.1043473590851364E-5 0.0 3.3214033264323692 3.1043473590851364E-5 74-75 0.0 3.1043473590851364E-5 0.0 3.6387607569516427 3.1043473590851364E-5 76-77 0.0 3.1043473590851364E-5 0.0 3.9818066618673456 3.1043473590851364E-5 78-79 0.0 3.1043473590851364E-5 0.0 4.365705778028609 3.1043473590851364E-5 80-81 3.1043473590851364E-5 3.1043473590851364E-5 0.0 4.765266326616457 3.1043473590851364E-5 82-83 3.1043473590851364E-5 3.1043473590851364E-5 0.0 5.18612269808763 3.1043473590851364E-5 84-85 3.1043473590851364E-5 3.1043473590851364E-5 0.0 5.612427199173995 3.1043473590851364E-5 86-87 3.1043473590851364E-5 3.1043473590851364E-5 0.0 6.05800969736028 3.1043473590851364E-5 88-89 3.1043473590851364E-5 3.1043473590851364E-5 0.0 6.5235997142758695 3.1043473590851364E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6410 0.0 43.653145 1 GTATCAA 11245 0.0 40.610798 1 TATCAAC 14400 0.0 31.749521 2 ATCAACG 14390 0.0 31.672504 3 TCAACGC 14400 0.0 31.617506 4 CAACGCA 14620 0.0 31.076714 5 AACGCAG 15265 0.0 29.852373 6 ACGCAGA 17205 0.0 26.452913 7 CGCAGAG 17300 0.0 26.115425 8 TACATGG 12030 0.0 26.073713 2 GTACATG 12460 0.0 25.86212 1 ACATGGG 12250 0.0 24.363974 3 GTGTAGC 1925 0.0 23.2773 1 GCAGAGT 18700 0.0 23.268913 9 TAAGGTG 1885 0.0 22.943233 5 GAGTACT 10370 0.0 21.482555 12-13 AGAGTAC 16400 0.0 20.839579 10-11 AGTACTT 10960 0.0 20.434454 12-13 GTACTTT 10920 0.0 20.031292 14-15 GTAAGGT 1915 0.0 19.853899 4 >>END_MODULE