FastQCFastQC Report
Thu 26 May 2016
SRR938433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221289
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105320.32694986385884656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82720.2567916135435225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62500.19402170994282103No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46300.1437312827256418No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA45560.1414340656799188No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC41660.12932711097948676No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40080.12442224215213227No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38820.120510764479685No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG37870.11756163448855411No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66200.047.4921571
GTATCAA112050.038.79831
ATCAACG143900.029.9674243
TATCAAC146700.029.6342872
TCAACGC145550.029.6277054
CAACGCA148900.029.0249925
AACGCAG155000.027.9145726
ACGCAGA174950.024.5685167
TAAGGTG20000.024.2478435
CGCAGAG177450.024.1956188
GTACATG125050.023.6583861
TACATGG122350.023.2085912
ACATGGG122050.022.5550443
GTAAGGT19650.022.2601554
GAGTACT108250.021.608412-13
GCAGAGT195900.021.6016839
AGTACTT113900.020.87010612-13
GTACTTT113050.020.56489214-15
AGAGTAC171450.020.37461910-11
CAGAGTA185000.019.49846310-11
Produced by FastQC (version 0.11.5)