##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938433_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3221289 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79819817470584 31.0 31.0 34.0 30.0 34.0 2 32.03086745709559 31.0 31.0 34.0 30.0 34.0 3 32.09988206584383 33.0 31.0 34.0 30.0 34.0 4 35.77230419251424 37.0 35.0 37.0 35.0 37.0 5 35.642980496316845 37.0 35.0 37.0 33.0 37.0 6 35.68527257256334 37.0 35.0 37.0 33.0 37.0 7 35.621043936138605 37.0 35.0 37.0 33.0 37.0 8 35.60213069985338 37.0 35.0 37.0 33.0 37.0 9 37.17806567495186 39.0 37.0 39.0 33.0 39.0 10-11 37.11083125419669 39.0 37.0 39.0 33.0 39.0 12-13 37.00682490766895 39.0 37.0 39.0 33.0 39.0 14-15 38.04899203393424 40.0 37.0 41.0 33.0 41.0 16-17 37.96908489117244 40.0 37.0 41.0 33.0 41.0 18-19 37.98274634781294 40.0 37.0 41.0 33.0 41.0 20-21 37.97946939874069 40.0 37.0 41.0 33.0 41.0 22-23 38.011297651343924 40.0 37.0 41.0 33.0 41.0 24-25 37.93201960457444 40.0 37.0 41.0 33.0 41.0 26-27 37.755600165027104 40.0 37.0 41.0 32.0 41.0 28-29 37.630465475156065 40.0 37.0 41.0 32.0 41.0 30-31 37.377572766678185 39.0 36.0 41.0 31.5 41.0 32-33 37.15380939120954 39.0 36.0 41.0 31.0 41.0 34-35 36.983072459503006 39.0 36.0 40.5 31.0 41.0 36-37 36.981326108896155 39.0 36.0 41.0 31.0 41.0 38-39 36.824152536453575 39.0 35.0 41.0 30.0 41.0 40-41 36.651595525890414 39.0 35.0 40.5 30.0 41.0 42-43 36.42524172776798 39.0 35.0 40.0 30.0 41.0 44-45 36.142679995492486 39.0 35.0 40.0 30.0 41.0 46-47 35.78342225736343 38.0 35.0 40.0 28.5 41.0 48-49 35.38603319975327 38.0 34.0 40.0 27.0 41.0 50-51 35.024346309815726 38.0 34.0 40.0 26.5 41.0 52-53 34.51054189797935 38.0 33.0 40.0 24.5 41.0 54-55 34.18925839935504 37.5 33.0 40.0 24.0 41.0 56-57 33.82497379154742 37.0 33.0 40.0 23.0 41.0 58-59 33.36643685183167 36.5 32.0 39.5 22.5 41.0 60-61 32.96129189277957 36.0 31.0 39.0 21.5 40.0 62-63 32.48325872034456 36.0 31.0 39.0 19.5 40.0 64-65 31.989825035878493 35.0 31.0 38.5 18.0 40.0 66-67 31.490073073232487 35.0 30.0 38.0 16.5 40.0 68-69 30.916048668716158 34.5 29.0 37.5 12.5 40.0 70-71 30.338579214718084 34.0 29.0 37.0 8.0 39.0 72-73 29.743890411571268 34.0 28.0 36.0 7.0 39.0 74-75 29.125657461966313 33.5 27.5 36.0 4.0 38.5 76-77 27.83401939409969 31.5 26.0 34.5 2.0 36.5 78-79 28.456692646949715 33.0 27.0 35.0 2.0 37.0 80-81 28.15208228755632 33.0 26.0 35.0 2.0 37.0 82-83 27.645373482478597 33.0 26.0 35.0 2.0 36.0 84-85 27.039668126641228 32.0 25.5 35.0 2.0 36.0 86-87 26.38741075389386 32.0 24.5 34.5 2.0 35.0 88-89 25.613563545524787 31.0 22.0 34.0 2.0 35.0 90-91 25.05015383593338 31.0 19.5 34.0 2.0 35.0 92-93 24.257793852088405 31.0 16.5 34.0 2.0 35.0 94-95 23.44816252127642 30.5 4.5 34.0 2.0 35.0 96-97 22.444441495314454 30.0 2.0 34.0 2.0 35.0 98-99 21.23494430335186 29.5 2.0 34.0 2.0 35.0 100-101 18.07396821582913 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2968772888958888 1101 2 0.3231397512477514 1101 3 0.31901641002451697 1101 4 0.20081268276152997 1101 5 0.13435228811102462 1101 6 0.1638374378331804 1101 7 0.28227100327320187 1101 8 0.22143097666011613 1101 9 0.27520377152658426 1101 10-11 0.36705101821262787 1101 12-13 0.4305937901361503 1101 14-15 0.5122100146180912 1101 16-17 0.47391188900461145 1101 18-19 0.5932337086697999 1101 20-21 0.6239927798523581 1101 22-23 0.5571796612573152 1101 24-25 0.5780669101841127 1101 26-27 0.5834220709354199 1101 28-29 0.5812052361490245 1101 30-31 0.5719136792102759 1101 32-33 0.5182705820956741 1101 34-35 0.4489877129369617 1101 36-37 0.5581829262725861 1101 38-39 0.7195636217161834 1101 40-41 0.7968202138760532 1101 42-43 0.6628178175593789 1101 44-45 0.7131416940165849 1101 46-47 0.844498899198399 1101 48-49 0.9053088638475586 1101 50-51 0.983706815245931 1101 52-53 1.0386963566900747 1101 54-55 1.0321914246021464 1101 56-57 1.0712222862407188 1101 58-59 1.276359976704775 1101 60-61 1.339888996340136 1101 62-63 1.3370485193127521 1101 64-65 1.3784020168167999 1101 66-67 1.4855383610773565 1101 68-69 1.4539336407368246 1101 70-71 1.591806431169143 1101 72-73 1.6876420049776613 1101 74-75 1.8484489355418425 1101 76-77 1.5837808652410352 1101 78-79 1.8067581284784389 1101 80-81 2.11849929280001 1101 82-83 2.345252112943694 1101 84-85 2.4774324706669226 1101 86-87 2.5402388982548594 1101 88-89 2.72578553225593 1101 90-91 3.020571700221879 1101 92-93 3.2959050311671447 1101 94-95 3.4412564578717166 1101 96-97 3.69428027169851 1101 98-99 4.066990634412775 1101 100-101 4.638317489995561 1102 1 0.32600684277791814 1102 2 0.35084914380863097 1102 3 0.31530385730206234 1102 4 0.24568761427012475 1102 5 0.17210156496921059 1102 6 0.1164311914170355 1102 7 0.21369740846198226 1102 8 0.2322061082273308 1102 9 0.3728884434686819 1102 10-11 0.3166782975428788 1102 12-13 0.394493722600437 1102 14-15 0.5081438551063897 1102 16-17 0.46134037123507454 1102 18-19 0.3680658250860773 1102 20-21 0.5572394635940938 1102 22-23 0.47265935594493413 1102 24-25 0.5111941321727258 1102 26-27 0.45235723665275884 1102 28-29 0.4473271572214301 1102 30-31 0.4787341489020278 1102 32-33 0.39020375996944523 1102 34-35 0.4563765020089221 1102 36-37 0.5582925125207865 1102 38-39 0.7318210347161695 1102 40-41 0.5955727146087568 1102 42-43 0.6792168898990454 1102 44-45 0.7074623229906791 1102 46-47 0.7558343860289369 1102 48-49 0.6647326440634203 1102 50-51 0.7795329808740732 1102 52-53 0.8749053733446388 1102 54-55 0.8296123249933416 1102 56-57 0.8249880392639 1102 58-59 0.9577306330754354 1102 60-61 1.0537831946402818 1102 62-63 1.3328839233164729 1102 64-65 1.3037992669665286 1102 66-67 1.3336479982959126 1102 68-69 1.4221443903832203 1102 70-71 1.5669615721658694 1102 72-73 1.565470540726988 1102 74-75 1.6777396831494222 1102 76-77 1.6324253725489086 1102 78-79 2.0274979630796643 1102 80-81 2.2974074442329098 1102 82-83 2.310645476604382 1102 84-85 2.467610484607313 1102 86-87 2.612179403116155 1102 88-89 2.8088933217993635 1102 90-91 2.9350402706310454 1102 92-93 3.278134856466277 1102 94-95 3.4772227282439268 1102 96-97 3.657368819935577 1102 98-99 4.037096843875421 1102 100-101 4.458200704185707 1103 1 0.32865901816024135 1103 2 0.3617911994359133 1103 3 0.3494978447545094 1103 4 0.33594412694043285 1103 5 0.20841008617175305 1103 6 0.27443065153607904 1103 7 0.2663088281249699 1103 8 0.2703568815884765 1103 9 0.4145751113812324 1103 10-11 0.46920891827976874 1103 12-13 0.5038501018476751 1103 14-15 0.5660004869902266 1103 16-17 0.554878767723487 1103 18-19 0.5818584806693039 1103 20-21 0.5940262843100541 1103 22-23 0.5182505927731214 1103 24-25 0.5267884368764797 1103 26-27 0.538786386234392 1103 28-29 0.490503688930076 1103 30-31 0.6502771498502824 1103 32-33 0.6100435097838925 1103 34-35 0.656566987156701 1103 36-37 0.7917085787429343 1103 38-39 0.8420767066216328 1103 40-41 0.8693406073263432 1103 42-43 0.8322296780747607 1103 44-45 0.908611544703021 1103 46-47 0.8723094784214638 1103 48-49 1.002378816018357 1103 50-51 0.9938364061866736 1103 52-53 1.171191869765586 1103 54-55 1.198295646539684 1103 56-57 1.0738858332028158 1103 58-59 1.3540199999041818 1103 60-61 1.4034160165524412 1103 62-63 1.3321668811963931 1103 64-65 1.3830404370017462 1103 66-67 1.4402088344518127 1103 68-69 1.4307669631073416 1103 70-71 1.5693491000200233 1103 72-73 1.7510231675165642 1103 74-75 1.9219674151974644 1103 76-77 1.789004438848 1103 78-79 2.090779665715999 1103 80-81 2.248765177661191 1103 82-83 2.4746049517391207 1103 84-85 2.6574631247921268 1103 86-87 2.729903937204181 1103 88-89 3.0255757248152015 1103 90-91 3.216024897866859 1103 92-93 3.431158841268182 1103 94-95 3.4227155670853904 1103 96-97 3.8541841330115645 1103 98-99 4.16220881013734 1103 100-101 4.267472945876488 1104 1 0.2963912329418541 1104 2 0.3493637331070545 1104 3 0.26560239746587655 1104 4 0.20432683699385734 1104 5 0.18377619559669256 1104 6 0.20740240057563142 1104 7 0.2295447447318537 1104 8 0.2556664945168663 1104 9 0.3097733644319902 1104 10-11 0.30378148856523524 1104 12-13 0.3049128570703985 1104 14-15 0.4589162529087858 1104 16-17 0.4873523971705538 1104 18-19 0.45050858190115406 1104 20-21 0.5512355592435867 1104 22-23 0.5258835187833029 1104 24-25 0.5053268682681065 1104 26-27 0.5078749295709457 1104 28-29 0.5338330708859544 1104 30-31 0.6346145941272354 1104 32-33 0.5213423344638102 1104 34-35 0.5716131628671448 1104 36-37 0.5457525252410633 1104 38-39 0.5945367926401346 1104 40-41 0.518998108334209 1104 42-43 0.6552241280005902 1104 44-45 0.6013262901859449 1104 46-47 0.7094738688325393 1104 48-49 0.8158252553519887 1104 50-51 0.854720297488214 1104 52-53 1.046552273941323 1104 54-55 1.1335844144828897 1104 56-57 1.1634669148676977 1104 58-59 1.2040186825631167 1104 60-61 1.254368512264783 1104 62-63 1.2433414786760508 1104 64-65 1.2844376999931981 1104 66-67 1.3029863551112975 1104 68-69 1.4635194523492139 1104 70-71 1.4532365270262488 1104 72-73 1.6646573182664923 1104 74-75 1.7179545327641499 1104 76-77 1.6473737585168884 1104 78-79 1.9294049001978664 1104 80-81 2.111158635280489 1104 82-83 2.1751014052826 1104 84-85 2.3457207048791986 1104 86-87 2.4148149382226016 1104 88-89 2.38153382600753 1104 90-91 2.831430756121346 1104 92-93 3.170721673884696 1104 94-95 3.4294755124777296 1104 96-97 3.819046287797981 1104 98-99 4.364481736860437 1104 100-101 4.537466841550437 1105 1 0.2340287369318581 1105 2 0.28859613107325544 1105 3 0.19880977374436526 1105 4 0.15109699669186938 1105 5 0.052876920194371735 1105 6 0.16742740490948194 1105 7 0.24497528395149715 1105 8 0.1770031814537063 1105 9 0.20669630275136797 1105 10-11 0.2880049797528912 1105 12-13 0.33793933777501906 1105 14-15 0.4779189189423505 1105 16-17 0.44400285951257246 1105 18-19 0.3622717972842082 1105 20-21 0.3202054098581968 1105 22-23 0.1663346195629245 1105 24-25 0.25540412988738836 1105 26-27 0.44013222793242335 1105 28-29 0.45160206178274365 1105 30-31 0.4998615146932437 1105 32-33 0.5261182933063537 1105 34-35 0.4957428527258898 1105 36-37 0.6030595544238011 1105 38-39 0.6116214874364303 1105 40-41 0.5866250098515806 1105 42-43 0.6754640414823498 1105 44-45 0.7360772465407877 1105 46-47 0.7872998117889054 1105 48-49 0.8424256370262384 1105 50-51 0.8437837168160982 1105 52-53 0.9786100028632561 1105 54-55 0.8945139336091543 1105 56-57 0.8932986783840065 1105 58-59 1.1849772011043314 1105 60-61 1.1238429449457286 1105 62-63 1.20415192998545 1105 64-65 1.30693121583257 1105 66-67 1.403404767856724 1105 68-69 1.3437944040214767 1105 70-71 1.3161286297441421 1105 72-73 1.4147840107067253 1105 74-75 1.658748363133082 1105 76-77 1.5809427505582967 1105 78-79 1.8628060305252028 1105 80-81 2.035702648649611 1105 82-83 2.0898313930001287 1105 84-85 2.334087108093282 1105 86-87 2.5148283966982206 1105 88-89 2.628479176658054 1105 90-91 2.916460814306639 1105 92-93 3.0044000229635834 1105 94-95 3.252669958281146 1105 96-97 3.569020857002144 1105 98-99 3.981571536277457 1105 100-101 4.44903634903854 1106 1 0.12789316640337134 1106 2 0.18340935680362236 1106 3 0.11681364794228699 1106 4 0.11057429437419586 1106 5 0.13116805664439823 1106 6 0.12726759355778228 1106 7 0.1612076929287909 1106 8 0.05353519042597554 1106 9 0.12940474346324748 1106 10-11 0.19915402784469194 1106 12-13 0.057210314664857265 1106 14-15 0.038594994253180914 1106 16-17 -0.00930419180078701 1106 18-19 0.2759178633728965 1106 20-21 0.36475822763459576 1106 22-23 0.28053303338383273 1106 24-25 0.22154160882454477 1106 26-27 0.05950777315112532 1106 28-29 0.2814917196468727 1106 30-31 0.34776748532666346 1106 32-33 0.30217066106678203 1106 34-35 0.33000434302806525 1106 36-37 0.36512375413463616 1106 38-39 0.2853028545023406 1106 40-41 0.3569834542803605 1106 42-43 0.4677222544913704 1106 44-45 0.018359807801218153 1106 46-47 -0.14767372943570223 1106 48-49 0.21440203068933528 1106 50-51 -0.01762520758845909 1106 52-53 0.22321134665060782 1106 54-55 0.32965732503834033 1106 56-57 0.22585905426534936 1106 58-59 0.5767603010181404 1106 60-61 0.3792951777609588 1106 62-63 0.6124249365531398 1106 64-65 0.4113941789092692 1106 66-67 0.5013213942301782 1106 68-69 0.5202835700441355 1106 70-71 0.6601854262592894 1106 72-73 1.0347406947797495 1106 74-75 1.3646503048426517 1106 76-77 1.391997322338252 1106 78-79 1.5603321480877597 1106 80-81 1.6003971983096221 1106 82-83 1.9158901633708076 1106 84-85 1.8727773897742175 1106 86-87 2.108777742105797 1106 88-89 2.246721455279669 1106 90-91 2.167885177388996 1106 92-93 1.9217226630975617 1106 94-95 2.6285546024018913 1106 96-97 3.1661379591394976 1106 98-99 3.6207959982267504 1106 100-101 4.102964723949729 1107 1 0.17895848357292365 1107 2 0.18546853818839537 1107 3 0.17976235019421694 1107 4 0.10274716609881551 1107 5 0.052840887409296045 1107 6 0.048877163313619576 1107 7 0.11206135226719027 1107 8 0.13340976659054604 1107 9 0.15776140192434696 1107 10-11 0.21151182607015784 1107 12-13 0.2745821164965463 1107 14-15 0.36585330121443604 1107 16-17 0.3674689156599342 1107 18-19 0.3295622217081302 1107 20-21 0.377919227435747 1107 22-23 0.3008700706255567 1107 24-25 0.21694763344054735 1107 26-27 0.2906295596562387 1107 28-29 0.2760025387898608 1107 30-31 0.30118814669209115 1107 32-33 0.3531533924106043 1107 34-35 0.3177555189986876 1107 36-37 0.4001126452526691 1107 38-39 0.44526626795975943 1107 40-41 0.4837514084566479 1107 42-43 0.5212735871573813 1107 44-45 0.5063935703439029 1107 46-47 0.599136431917195 1107 48-49 0.6239893876568132 1107 50-51 0.6273612508239097 1107 52-53 0.6640711881533505 1107 54-55 0.752214166210031 1107 56-57 0.6500296731161725 1107 58-59 0.6638916346046386 1107 60-61 0.7642772007947158 1107 62-63 0.9099782903728553 1107 64-65 1.0180518847843132 1107 66-67 1.1717261616224697 1107 68-69 1.2279562241757667 1107 70-71 1.2174624947299115 1107 72-73 1.4228887003076238 1107 74-75 1.502650153442712 1107 76-77 1.5127898210752484 1107 78-79 1.6852453196873327 1107 80-81 1.8632738345440707 1107 82-83 1.9293178456420357 1107 84-85 2.161762075456739 1107 86-87 2.366523972700353 1107 88-89 2.671510823662917 1107 90-91 2.7485384770281414 1107 92-93 2.9993508959172672 1107 94-95 3.0898800022834365 1107 96-97 3.3529723302673133 1107 98-99 3.7872671214419604 1107 100-101 3.9266038259348264 1108 1 0.17179051464854567 1108 2 0.16841671137619585 1108 3 0.16977478785995004 1108 4 0.11439949385157178 1108 5 0.02655850638267765 1108 6 0.0019440334516147573 1108 7 0.03049557308623463 1108 8 0.028034545360114294 1108 9 0.12369981384527051 1108 10-11 0.09190771951143262 1108 12-13 0.19396633185565548 1108 14-15 0.22973889735360586 1108 16-17 0.2693673423055216 1108 18-19 0.27912983941095604 1108 20-21 0.24235081718370566 1108 22-23 0.1901765625925762 1108 24-25 0.17741314621782323 1108 26-27 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-0.06322847395261277 2316 58-59 0.13609168643648673 2316 60-61 0.29976131561839736 2316 62-63 0.3454445608771053 2316 64-65 0.2991462623135206 2316 66-67 0.4508888655367791 2316 68-69 0.5767206699594958 2316 70-71 0.7072447124490076 2316 72-73 0.7692869945434424 2316 74-75 0.887570392980134 2316 76-77 0.9496588147823495 2316 78-79 1.0109649465466468 2316 80-81 1.0766248484503151 2316 82-83 1.2614300523889561 2316 84-85 1.3650958820927102 2316 86-87 1.5668249827617338 2316 88-89 1.770085282991321 2316 90-91 1.9318227674135393 2316 92-93 1.9545282828597053 2316 94-95 2.5357936618148607 2316 96-97 2.716620379187134 2316 98-99 2.957859639638219 2316 100-101 3.4257905467755485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 22.0 4 41.0 5 111.0 6 218.0 7 608.0 8 1415.0 9 2448.0 10 4190.0 11 6463.0 12 9249.0 13 12978.0 14 16687.0 15 22128.0 16 27426.0 17 34457.0 18 33349.0 19 29577.0 20 29824.0 21 31558.0 22 34952.0 23 39630.0 24 45631.0 25 53584.0 26 63176.0 27 75522.0 28 89907.0 29 106487.0 30 126453.0 31 149893.0 32 178658.0 33 216525.0 34 269770.0 35 341702.0 36 426466.0 37 472640.0 38 253723.0 39 13817.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.92889363785344 19.694581699798867 15.24825326026523 23.128271402082458 2 15.737873744454046 20.745398790154024 39.62590305206909 23.89082441332284 3 19.17760122982016 24.444972874170674 30.140062161548002 26.237363734461166 4 13.148897848035368 17.26606957649562 36.868222627649985 32.71680994781902 5 13.411733557736468 37.866071600593905 34.46814157037033 14.254053271299291 6 32.149862989629305 36.879429321616286 17.01663526619313 13.954072422561278 7 28.60690239217903 32.855853666032445 20.47118405085666 18.06605989093186 8 26.50755644712412 35.345385030650775 19.530163235897184 18.616895286327924 9 26.376584032044313 16.716072354886506 19.899797875943452 37.00754573712573 10-11 24.76139210111232 26.837657223552437 27.517121251772192 20.883829423563053 12-13 25.954299660787967 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28.808308042300183 22.23115962905381 38-39 21.931134134117507 26.894739628198405 28.82287458615793 22.35125165152616 40-41 22.389421316415007 26.65229451263232 28.982424484487712 21.97585968646496 42-43 22.19355878226655 26.69227179915923 28.671875654861896 22.442293763712325 44-45 22.08395072766383 26.901240811263133 28.902576338810487 22.112232122262547 46-47 22.589620235638737 27.116785930592336 28.10499343895778 22.188600394811143 48-49 22.226180265105057 27.232126642471382 28.02289704525114 22.518796047172422 50-51 22.465339185648975 27.2843262433144 28.026513609924475 22.22382096111215 52-53 22.779297355810048 27.009032719510728 28.027491479342586 22.184178445336634 54-55 22.182921184656205 27.270636071460835 28.211874190735447 22.334568553147513 56-57 22.310665078482558 27.436594481277528 27.87899502342075 22.37374541681917 58-59 22.225761178211577 26.985734592580794 28.00520226530436 22.78330196390327 60-61 21.869257927494242 27.362260883764232 28.19483132373407 22.573649865007457 62-63 22.00763421102546 27.336727626735758 28.32425156513433 22.33138659710445 64-65 21.690788997820437 27.304659718516405 28.155375068800097 22.849176214863057 66-67 21.7684125695821 27.431727933375644 27.96620416482928 22.833655332212974 68-69 22.307212565435925 26.97925142202745 28.100327167980687 22.613208844555942 70-71 22.094090284975984 27.022536630522747 27.807362208109858 23.076010876391408 72-73 22.04027984454047 27.140149042844193 27.85289178538339 22.966679327231944 74-75 22.060392594393115 27.553085115927196 27.525844467851222 22.860677821828467 76-77 22.108199543723025 27.128053397258057 27.922098886501644 22.841648172517274 78-79 22.09382641545046 27.332893757747286 27.840423507484115 22.732856319318138 80-81 22.28071850153083 27.3267443529955 27.621704054667585 22.770833090806082 82-83 22.265091980440545 27.36991834395005 27.69243234509029 22.672557330519112 84-85 22.090109746144325 27.462101397601675 27.695588640244928 22.752200216009072 86-87 22.34248780093321 27.46330966809318 27.371435409944056 22.82276712102955 88-89 22.06467819611823 27.580843864710573 27.176005611422642 23.178472327748555 90-91 21.944439486071065 27.45842662620634 27.534468240325026 23.062665647397566 92-93 22.1260941876411 27.209706072038074 27.649344632145738 23.014855108175084 94-95 21.8276596728825 26.922871558559326 27.869464677028354 23.38000409152982 96-97 22.04714045326893 26.67105104060068 27.806512828639846 23.475295677490546 98-99 21.77553768072346 26.96437668275029 27.434995742387596 23.82508989413865 100-101 21.881447887525944 26.548614462328928 27.95030265345051 23.619634996694614 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1042.0 1 669.5 2 420.5 3 591.0 4 552.5 5 524.0 6 896.0 7 1996.0 8 3100.5 9 3325.5 10 2774.0 11 2249.5 12 2209.5 13 2303.5 14 2524.0 15 2713.5 16 2969.5 17 3296.0 18 3577.5 19 4039.5 20 4733.0 21 5640.0 22 6719.0 23 8253.0 24 10163.0 25 12595.0 26 16565.5 27 21531.5 28 25400.5 29 30142.0 30 36314.5 31 44474.0 32 57572.5 33 64067.5 34 66025.0 35 72445.5 36 81188.5 37 89196.5 38 93111.5 39 96096.5 40 99978.5 41 105232.5 42 107879.5 43 110034.0 44 113499.5 45 118929.5 46 121213.0 47 121484.0 48 122782.5 49 120215.5 50 115806.0 51 110460.5 52 106500.5 53 106258.0 54 107106.0 55 100166.0 56 89932.0 57 81275.0 58 74544.0 59 66102.5 60 53375.0 61 44079.0 62 37245.0 63 27654.0 64 18582.5 65 13617.5 66 10465.0 67 8306.0 68 6440.0 69 4991.0 70 3886.5 71 2636.0 72 1893.5 73 1589.0 74 1190.5 75 841.5 76 608.0 77 409.0 78 250.5 79 139.0 80 88.0 81 53.0 82 30.5 83 16.5 84 5.5 85 3.5 86 2.5 87 1.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.208694718170273E-5 2 0.04268477618742063 3 1.5521736795425683E-4 4 0.0 5 0.10154320211567482 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012727824172249057 26-27 0.012355302489158842 28-29 0.0 30-31 0.0 32-33 5.587825246353245E-4 34-35 0.015614867216198237 36-37 0.003756260304493015 38-39 0.012557085067499378 40-41 0.007621172766554009 42-43 0.005898259982261759 44-45 0.0027939126231766227 46-47 6.208694718170273E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.3969563115883113E-4 68-69 2.483477887268109E-4 70-71 0.0 72-73 1.5521736795425682E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.656521038627704E-5 82-83 0.0010399563652935208 84-85 0.003973564619628975 86-87 0.0011951737332477775 88-89 7.760868397712841E-5 90-91 8.226520501575612E-4 92-93 2.0178257834053387E-4 94-95 0.0 96-97 1.5521736795425682E-5 98-99 0.0 100-101 9.468259445209666E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3221289.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.48136244737053 #Duplication Level Percentage of deduplicated Percentage of total 1 69.23677219000109 32.18219502849465 2 15.747285300737657 14.639105512514746 3 6.0458131388091605 8.430528953821902 4 3.0324246621178386 5.638049192569775 5 1.6716472008927512 3.8850219714414194 6 1.0307352446098397 2.874598709519345 7 0.6914339366597243 2.2497153992805017 8 0.4653174669057622 1.730287186587126 9 0.32583365614982196 1.3630673042146597 >10 1.5763574813704238 12.949833131607825 >50 0.10702439406473578 3.3892066441786017 >100 0.061865280654221096 5.3640204929387565 >500 0.003745023514258092 1.1800649092632514 >1k 0.0035443972530687946 3.3455118373870225 >5k 1.3375083973844507E-4 0.451410652653953 >10k+ 6.687541986922253E-5 0.32738307352647245 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10532 0.32694986385884656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8272 0.2567916135435225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6250 0.19402170994282103 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4630 0.1437312827256418 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4556 0.1414340656799188 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4166 0.12932711097948676 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4008 0.12442224215213227 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3882 0.120510764479685 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3787 0.11756163448855411 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.1043473590851364E-5 0.0 8 0.0 0.0 0.0 3.1043473590851364E-5 0.0 9 0.0 0.0 0.0 3.1043473590851364E-5 0.0 10-11 0.0 0.0 0.0 4.656521038627704E-5 0.0 12-13 0.0 0.0 0.0 9.31304207725541E-5 0.0 14-15 0.0 0.0 0.0 9.31304207725541E-5 0.0 16-17 0.0 0.0 0.0 9.31304207725541E-5 0.0 18-19 0.0 0.0 0.0 1.5521736795425683E-4 0.0 20-21 0.0 0.0 0.0 2.7939126231766226E-4 0.0 22-23 0.0 0.0 0.0 6.984781557941558E-4 0.0 24-25 0.0 0.0 0.0 0.0014124780483837371 1.5521736795425682E-5 26-27 0.0 0.0 0.0 0.0020643909937916156 3.1043473590851364E-5 28-29 0.0 0.0 0.0 0.004858303616968238 3.1043473590851364E-5 30-31 0.0 0.0 0.0 0.014373128272564182 3.1043473590851364E-5 32-33 0.0 0.0 0.0 0.03515673384163917 3.1043473590851364E-5 34-35 0.0 0.0 0.0 0.06550172927669638 3.1043473590851364E-5 36-37 0.0 0.0 0.0 0.11261020045081332 3.1043473590851364E-5 38-39 0.0 0.0 0.0 0.18776645001426448 3.1043473590851364E-5 40-41 0.0 0.0 0.0 0.26698939461811716 3.1043473590851364E-5 42-43 0.0 0.0 0.0 0.348478512794102 3.1043473590851364E-5 44-45 0.0 0.0 0.0 0.45565610536651635 3.1043473590851364E-5 46-47 0.0 0.0 0.0 0.5822358689332128 3.1043473590851364E-5 48-49 0.0 0.0 0.0 0.7154744575851468 3.1043473590851364E-5 50-51 0.0 0.0 0.0 0.8593143924683566 3.1043473590851364E-5 52-53 0.0 0.0 0.0 0.9989945018903923 3.1043473590851364E-5 54-55 0.0 0.0 0.0 1.1553449566307152 3.1043473590851364E-5 56-57 0.0 0.0 0.0 1.339401711550873 3.1043473590851364E-5 58-59 0.0 0.0 0.0 1.5336562475456255 3.1043473590851364E-5 60-61 0.0 0.0 0.0 1.7441309984915976 3.1043473590851364E-5 62-63 0.0 0.0 0.0 1.9551179667518188 3.1043473590851364E-5 64-65 0.0 0.0 0.0 2.1774202811359054 3.1043473590851364E-5 66-67 0.0 0.0 0.0 2.4369747638290136 3.1043473590851364E-5 68-69 0.0 0.0 0.0 2.7214261123419847 3.1043473590851364E-5 70-71 0.0 0.0 0.0 3.022578849646834 3.1043473590851364E-5 72-73 0.0 0.0 0.0 3.335807498178524 3.1043473590851364E-5 74-75 0.0 0.0 0.0 3.6591563191008323 6.208694718170273E-5 76-77 0.0 0.0 0.0 4.009249092521658 6.208694718170273E-5 78-79 0.0 0.0 0.0 4.403113163705585 6.208694718170273E-5 80-81 0.0 0.0 0.0 4.81496692783541 6.208694718170273E-5 82-83 0.0 0.0 0.0 5.2496531667913064 6.208694718170273E-5 84-85 0.0 0.0 0.0 5.693140230510209 6.208694718170273E-5 86-87 0.0 0.0 0.0 6.157488508482164 6.208694718170273E-5 88-89 0.0 0.0 0.0 6.6453522176991875 6.208694718170273E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6620 0.0 47.492157 1 GTATCAA 11205 0.0 38.7983 1 ATCAACG 14390 0.0 29.967424 3 TATCAAC 14670 0.0 29.634287 2 TCAACGC 14555 0.0 29.627705 4 CAACGCA 14890 0.0 29.024992 5 AACGCAG 15500 0.0 27.914572 6 ACGCAGA 17495 0.0 24.568516 7 TAAGGTG 2000 0.0 24.247843 5 CGCAGAG 17745 0.0 24.195618 8 GTACATG 12505 0.0 23.658386 1 TACATGG 12235 0.0 23.208591 2 ACATGGG 12205 0.0 22.555044 3 GTAAGGT 1965 0.0 22.260155 4 GAGTACT 10825 0.0 21.6084 12-13 GCAGAGT 19590 0.0 21.601683 9 AGTACTT 11390 0.0 20.870106 12-13 GTACTTT 11305 0.0 20.564892 14-15 AGAGTAC 17145 0.0 20.374619 10-11 CAGAGTA 18500 0.0 19.498463 10-11 >>END_MODULE