FastQCFastQC Report
Thu 26 May 2016
SRR938432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204842
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98530.30744105325629156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72950.2276243259418093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54880.17124089112661403No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA43920.13704263735934563No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA42830.1336415336543892No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42340.13211259712647302No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA37500.11701044856501507No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35040.10933456313915009No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32950.10281318080579323No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA32700.10203311114869314No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61750.044.028461
GTATCAA106300.039.9797441
ATCAACG137100.030.6460763
TATCAAC139000.030.329752
TCAACGC138800.030.3049684
CAACGCA141850.029.6193945
AACGCAG147450.028.5509226
GTACATG122300.027.1832831
TACATGG119800.027.0971682
ACATGGG124000.025.1827833
ACGCAGA168050.024.8186827
CGCAGAG169000.024.6229528
GTGTAAG16800.024.1326621
TAAGGTG21800.023.1101425
GTAAGGT21900.023.0049764
GTATAGG8050.022.5155891
GCAGAGT182600.022.137989
AGGTAAG21550.021.1730792
GAGTACT100800.020.87624512-13
AGAGTAC160350.020.5434410-11
Produced by FastQC (version 0.11.5)