##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938432_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3204842 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13215628102727 31.0 31.0 34.0 28.0 34.0 2 31.31129615750168 31.0 31.0 34.0 28.0 34.0 3 31.366386548853267 31.0 31.0 34.0 28.0 34.0 4 35.03921285355097 37.0 35.0 37.0 32.0 37.0 5 35.03550939484692 37.0 35.0 37.0 32.0 37.0 6 35.01348615625981 37.0 35.0 37.0 32.0 37.0 7 34.932244085667875 37.0 35.0 37.0 32.0 37.0 8 34.88794517795261 37.0 35.0 37.0 32.0 37.0 9 36.379740093271366 39.0 37.0 39.0 32.0 39.0 10-11 36.21266305796043 39.0 35.0 39.0 32.0 39.0 12-13 36.036607576910185 38.0 35.0 39.0 30.5 39.0 14-15 37.199641043146585 39.0 36.5 41.0 31.5 41.0 16-17 37.12783344701548 39.0 36.0 41.0 31.0 41.0 18-19 37.1343169179635 39.5 36.0 41.0 31.0 41.0 20-21 37.05818695586241 39.0 36.0 41.0 31.0 41.0 22-23 36.91551127949521 39.0 36.0 41.0 30.5 41.0 24-25 36.76942981900512 39.0 36.0 41.0 30.0 41.0 26-27 36.54935516321865 39.0 36.0 41.0 30.0 41.0 28-29 36.32241308619894 39.0 35.5 40.0 29.5 41.0 30-31 36.038896145270186 38.5 35.0 40.0 29.0 41.0 32-33 35.73169176514786 38.0 35.0 40.0 27.5 41.0 34-35 35.429465477549286 38.0 34.0 40.0 27.0 41.0 36-37 35.18422078217897 38.0 34.0 40.0 25.5 41.0 38-39 34.92008810418735 38.0 34.0 40.0 25.0 41.0 40-41 34.63859341583766 38.0 33.5 40.0 24.5 41.0 42-43 34.20925228139172 38.0 33.0 40.0 23.5 41.0 44-45 33.73542315034563 38.0 33.0 40.0 22.5 41.0 46-47 33.249193095946694 37.0 31.5 40.0 19.0 41.0 48-49 32.742534733381554 37.0 31.0 40.0 16.0 41.0 50-51 32.025125107571604 36.0 30.5 38.5 14.0 40.5 52-53 32.02207909157456 36.0 31.0 39.0 12.5 40.0 54-55 31.982814129370496 36.0 30.5 39.0 8.5 40.0 56-57 31.551525160990778 36.0 30.0 39.0 7.5 40.0 58-59 31.089700521897804 35.5 29.0 39.0 3.5 40.0 60-61 30.52122663145328 35.0 28.5 39.0 2.0 40.0 62-63 29.7827608974171 34.5 27.5 38.0 2.0 40.0 64-65 29.1031280169194 34.0 26.5 38.0 2.0 40.0 66-67 28.381706180835124 34.0 26.0 37.5 2.0 39.5 68-69 27.497830626283605 33.0 24.0 36.5 2.0 39.0 70-71 26.621751555926938 32.0 21.5 36.0 2.0 39.0 72-73 25.68057598471313 31.5 19.0 35.5 2.0 38.0 74-75 24.711341619961296 31.0 10.5 35.0 2.0 37.5 76-77 23.72329447130311 30.0 6.5 34.5 2.0 36.5 78-79 22.696297196554465 29.5 2.0 34.0 2.0 36.0 80-81 21.688388226315055 29.0 2.0 34.0 2.0 35.5 82-83 20.636024334428967 27.0 2.0 33.5 2.0 35.0 84-85 19.60283361863081 26.0 2.0 33.0 2.0 35.0 86-87 18.566137269793643 24.5 2.0 32.0 2.0 34.5 88-89 17.415827987775998 21.0 2.0 32.0 2.0 34.0 90-91 16.21648259102945 14.0 2.0 31.0 2.0 34.0 92-93 15.021333657010237 4.0 2.0 31.0 2.0 34.0 94-95 13.729869990470668 2.0 2.0 30.0 2.0 34.0 96-97 12.303833543120067 2.0 2.0 29.0 2.0 34.0 98-99 10.62631792768567 2.0 2.0 26.0 2.0 33.5 100-101 7.464325074371841 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5706687026752064 1101 2 0.5208226893258043 1101 3 0.4402417281988278 1101 4 0.33475725025408565 1101 5 0.37505825193880327 1101 6 0.45984201488964516 1101 7 0.4698270381770584 1101 8 0.35992068049091586 1101 9 0.320275575776634 1101 10-11 0.4851505586093481 1101 12-13 0.40955363786344634 1101 14-15 0.5488654103145976 1101 16-17 0.530925255368814 1101 18-19 0.49075762664026 1101 20-21 0.6403601863382704 1101 22-23 0.6342083706430088 1101 24-25 0.786223372827429 1101 26-27 0.7716271704417395 1101 28-29 0.7815879655816431 1101 30-31 0.8323379457528617 1101 32-33 0.8841761933433787 1101 34-35 0.9234163013656058 1101 36-37 1.0709403775324873 1101 38-39 1.0453201011554185 1101 40-41 0.7417195713453211 1101 42-43 1.1509887614576826 1101 44-45 1.288346476801685 1101 46-47 1.2371740922442456 1101 48-49 1.0978839092679422 1101 50-51 1.2474700487128345 1101 52-53 1.2351460797011882 1101 54-55 1.5305244872196155 1101 56-57 1.7955511889030866 1101 58-59 1.739456803533173 1101 60-61 1.7471867634045175 1101 62-63 1.6940018096466574 1101 64-65 1.8847109572667797 1101 66-67 2.1411330181631207 1101 68-69 2.3213137689479524 1101 70-71 2.6772945761681157 1101 72-73 2.9427019182893375 1101 74-75 2.748164594750211 1101 76-77 3.033090523331861 1101 78-79 3.2922360604171885 1101 80-81 3.765138244818665 1101 82-83 3.796208494219197 1101 84-85 3.927075494518217 1101 86-87 4.185315587162652 1101 88-89 4.055623319299585 1101 90-91 4.096734831866577 1101 92-93 4.100776276326085 1101 94-95 4.069843417363819 1101 96-97 4.029584166335555 1101 98-99 3.9282437688630925 1101 100-101 2.8257239037820954 1102 1 0.6713588886595083 1102 2 0.5741373485228145 1102 3 0.37751177135122305 1102 4 0.23486071506343364 1102 5 0.36705991746988076 1102 6 0.5016859596747665 1102 7 0.5046265435647967 1102 8 0.5153513212156753 1102 9 0.5228596724783756 1102 10-11 0.6522575815987253 1102 12-13 0.6838255989001141 1102 14-15 0.8351878672252866 1102 16-17 0.9107269057587004 1102 18-19 0.7448848631204399 1102 20-21 0.848228558912524 1102 22-23 0.8553826962290998 1102 24-25 0.7757172784978081 1102 26-27 0.8539227012666899 1102 28-29 0.8275906909961321 1102 30-31 0.6597983893897705 1102 32-33 0.7544748533479151 1102 34-35 0.8152804344062048 1102 36-37 0.9244777384227874 1102 38-39 1.1047719900190671 1102 40-41 1.0719420206854195 1102 42-43 1.3001522106211283 1102 44-45 1.295532233987437 1102 46-47 1.3520225541515316 1102 48-49 1.5216423315557748 1102 50-51 1.5135399000768004 1102 52-53 1.4639414717318715 1102 54-55 1.3409359491016701 1102 56-57 1.681965754729415 1102 58-59 1.9753540453127627 1102 60-61 2.1442165663166755 1102 62-63 2.0194396424374297 1102 64-65 2.2308707725442396 1102 66-67 2.342626949068819 1102 68-69 2.47899476927601 1102 70-71 2.3480935263788325 1102 72-73 2.57308082660942 1102 74-75 2.5453906022703343 1102 76-77 2.8285601526250197 1102 78-79 3.4154115468279684 1102 80-81 3.4802870070262486 1102 82-83 3.6105301688349876 1102 84-85 3.71827593865984 1102 86-87 3.933476563264808 1102 88-89 4.258991603550225 1102 90-91 3.9526802731061323 1102 92-93 3.8942056300495604 1102 94-95 3.829372904834484 1102 96-97 3.899272762494741 1102 98-99 3.7949400870300014 1102 100-101 2.7170196112542735 1103 1 0.5543165784921982 1103 2 0.47315520566567315 1103 3 0.44066452231647446 1103 4 0.3166649299926476 1103 5 0.32664526174272623 1103 6 0.4248624397262475 1103 7 0.41861584536659535 1103 8 0.41717966741902046 1103 9 0.5567625038812096 1103 10-11 0.7496532138381085 1103 12-13 0.7321006149876368 1103 14-15 0.6820465786152496 1103 16-17 0.7340196181139191 1103 18-19 0.7968503553984263 1103 20-21 0.849733143854607 1103 22-23 0.8848292856756856 1103 24-25 0.7975683483176326 1103 26-27 0.8933877913567798 1103 28-29 0.9328165195032128 1103 30-31 0.7591516712430533 1103 32-33 1.1465873453041553 1103 34-35 1.2189626657446837 1103 36-37 1.2315469951795492 1103 38-39 1.1661840717436505 1103 40-41 1.2578419161165684 1103 42-43 1.2406241781243494 1103 44-45 1.3577909212461279 1103 46-47 1.4327786674076464 1103 48-49 1.3225959190718655 1103 50-51 1.5845033411964948 1103 52-53 1.6379289636881111 1103 54-55 1.690656227415694 1103 56-57 1.8595922428246574 1103 58-59 1.9784907087619246 1103 60-61 2.211585251966614 1103 62-63 2.284434816182607 1103 64-65 2.5853819131491313 1103 66-67 2.757500460973578 1103 68-69 2.680064790189782 1103 70-71 2.777984935645243 1103 72-73 3.111194565348157 1103 74-75 3.0329235816783147 1103 76-77 3.142769853397219 1103 78-79 3.3345675555152283 1103 80-81 3.2964893477598416 1103 82-83 3.744786925591743 1103 84-85 3.745898002688154 1103 86-87 4.0758742064437 1103 88-89 4.222521807861678 1103 90-91 3.919566735461345 1103 92-93 3.9883998465875248 1103 94-95 3.8801589810893073 1103 96-97 3.6759311843094142 1103 98-99 3.0388095368369488 1103 100-101 2.185454295938958 1104 1 0.43471201778445234 1104 2 0.33411730145578744 1104 3 0.3777143621578425 1104 4 0.370168209431327 1104 5 0.28897156589159323 1104 6 0.41919930671831196 1104 7 0.4243356058913079 1104 8 0.39617943862097604 1104 9 0.56490923969573 1104 10-11 0.4414524244848508 1104 12-13 0.4326769954025167 1104 14-15 0.537578438743374 1104 16-17 0.5382743084738877 1104 18-19 0.5502348505174055 1104 20-21 0.5124873003524755 1104 22-23 0.5910312380856979 1104 24-25 0.5887494229057921 1104 26-27 0.5251598322353601 1104 28-29 0.7277878244147402 1104 30-31 0.6625180531832697 1104 32-33 0.8300186486903698 1104 34-35 0.7252339248966138 1104 36-37 0.8298769600971809 1104 38-39 0.8416979140255307 1104 40-41 0.9081144717907605 1104 42-43 1.0876676306868873 1104 44-45 1.0160429041625392 1104 46-47 1.0011236948634092 1104 48-49 1.0759827021713164 1104 50-51 1.1116150518432377 1104 52-53 1.141986792394647 1104 54-55 1.4527612647860408 1104 56-57 1.7329041898960433 1104 58-59 1.8819392673342321 1104 60-61 1.988728613009929 1104 62-63 2.0928187393064626 1104 64-65 2.0830375911540138 1104 66-67 2.3827501368430113 1104 68-69 2.6747102485485392 1104 70-71 2.681627781892953 1104 72-73 2.868256417087025 1104 74-75 2.680838850134691 1104 76-77 2.72579361026116 1104 78-79 3.1185008799508616 1104 80-81 3.4950596459599623 1104 82-83 3.50837905493891 1104 84-85 3.589580030601514 1104 86-87 3.4656884033625524 1104 88-89 3.5654232531026757 1104 90-91 3.389791434210583 1104 92-93 3.427933956563118 1104 94-95 3.6672964315163963 1104 96-97 3.36219481685975 1104 98-99 3.033413906879659 1104 100-101 2.1246298603074747 1105 1 0.5410595652908796 1105 2 0.49376145379048353 1105 3 0.4268568603002727 1105 4 0.4136363146311126 1105 5 0.35767239570611764 1105 6 0.417381004082678 1105 7 0.40446314884694345 1105 8 0.5273962515774357 1105 9 0.44794687231805597 1105 10-11 0.5170202161633455 1105 12-13 0.5192200456807043 1105 14-15 0.5871568865210364 1105 16-17 0.677462556157721 1105 18-19 0.7396005991728103 1105 20-21 0.7560029270829318 1105 22-23 0.7582481275598738 1105 24-25 0.7796430089086854 1105 26-27 0.603628752952936 1105 28-29 0.6978019399202537 1105 30-31 0.8006375698329222 1105 32-33 0.843790831277758 1105 34-35 0.8454487987329884 1105 36-37 1.0152053922692588 1105 38-39 1.0086912912156052 1105 40-41 1.075946523452302 1105 42-43 1.2466533279143235 1105 44-45 1.4569133334873499 1105 46-47 1.5532624657874834 1105 48-49 1.459180921322094 1105 50-51 1.4633604612835072 1105 52-53 1.5640585297697243 1105 54-55 1.7825788102989861 1105 56-57 1.930111997925163 1105 58-59 1.9728116096020116 1105 60-61 2.172988314147826 1105 62-63 2.127778275528538 1105 64-65 2.33097161274409 1105 66-67 2.472218479650447 1105 68-69 2.667625311866953 1105 70-71 2.6559872983815147 1105 72-73 2.83267165055668 1105 74-75 2.9378648883089795 1105 76-77 3.058060226184498 1105 78-79 3.3630051961542904 1105 80-81 3.3622747231723835 1105 82-83 3.628012998199008 1105 84-85 3.849998522115097 1105 86-87 3.8884991408121365 1105 88-89 3.986592886844573 1105 90-91 3.9357000519179905 1105 92-93 3.9180868309109567 1105 94-95 4.055005168833082 1105 96-97 3.9055083764036223 1105 98-99 3.6808781499482617 1105 100-101 2.576359261435509 1106 1 0.23307502697522864 1106 2 0.186537039549723 1106 3 0.05473115268595308 1106 4 0.19731170830445421 1106 5 0.19383501467153508 1106 6 -0.07053050860658772 1106 7 0.19400719787486054 1106 8 0.013927752429538032 1106 9 0.16749470574362846 1106 10-11 0.20804757963864517 1106 12-13 0.41071215373801095 1106 14-15 0.5338863296666148 1106 16-17 -2.8923997561491888E-6 1106 18-19 0.32566540871774663 1106 20-21 0.47477472916551733 1106 22-23 0.274395221392119 1106 24-25 0.3746374287471852 1106 26-27 0.371318288503069 1106 28-29 0.5027484153428006 1106 30-31 0.2955116892664833 1106 32-33 0.5301499297889904 1106 34-35 0.31666998213098907 1106 36-37 0.45267486766378084 1106 38-39 0.5065251088418776 1106 40-41 0.6394593781922637 1106 42-43 0.27396362878520364 1106 44-45 0.7007993321803454 1106 46-47 1.009811560168231 1106 48-49 0.7960388636498195 1106 50-51 0.5950921535275242 1106 52-53 0.8058526551119627 1106 54-55 0.7066260663312889 1106 56-57 1.2028786557865025 1106 58-59 1.2310981922404522 1106 60-61 1.5107391363913614 1106 62-63 1.5188081264860003 1106 64-65 1.549457038937657 1106 66-67 1.7376047058669393 1106 68-69 1.966865584347186 1106 70-71 1.943189013446382 1106 72-73 2.1251268648374975 1106 74-75 2.3401335170943547 1106 76-77 2.5119848015798567 1106 78-79 2.862030616869987 1106 80-81 2.774610264326377 1106 82-83 2.9981069916776555 1106 84-85 2.980749731811013 1106 86-87 2.5458278805950947 1106 88-89 2.2605770140046637 1106 90-91 2.3205251219888297 1106 92-93 2.5911806647761697 1106 94-95 2.548275673414329 1106 96-97 2.3718158812904893 1106 98-99 2.348269115539262 1106 100-101 1.5103330132714907 1107 1 0.20376300706362827 1107 2 0.16413734852281436 1107 3 0.10904845088790438 1107 4 0.044647072849791414 1107 5 0.036189904599865486 1107 6 0.16602315401195966 1107 7 0.05128548822374057 1107 8 0.2078403817047345 1107 9 0.2396910753097785 1107 10-11 0.333639820980963 1107 12-13 0.23934168641620346 1107 14-15 0.3842921143295328 1107 16-17 0.30093926097105594 1107 18-19 0.311877141112717 1107 20-21 0.45788492956889115 1107 22-23 0.41882928567569166 1107 24-25 0.4016799554604873 1107 26-27 0.4114413627853466 1107 28-29 0.34966919807464336 1107 30-31 0.5166516712430607 1107 32-33 0.628716809589875 1107 34-35 0.5493332014589711 1107 36-37 0.6532344951795466 1107 38-39 0.7473537146007985 1107 40-41 0.6716588804022834 1107 42-43 0.7796554281243502 1107 44-45 0.7330766355318374 1107 46-47 0.763278667407647 1107 48-49 0.7294441333575818 1107 50-51 1.0052310197679262 1107 52-53 1.0126119994024023 1107 54-55 1.0636785488442726 1107 56-57 1.305632421396087 1107 58-59 1.2836603516190692 1107 60-61 1.429866501966611 1107 62-63 1.541613387611175 1107 64-65 1.6559042345777009 1107 66-67 1.6983888538307212 1107 68-69 1.8069085401897844 1107 70-71 2.0127885070738145 1107 72-73 1.879533851062444 1107 74-75 2.0489414388211706 1107 76-77 2.1741894962543604 1107 78-79 2.5052416626580865 1107 80-81 2.5033063120455523 1107 82-83 2.6087154970203166 1107 84-85 2.5348756812595816 1107 86-87 2.8272268850151256 1107 88-89 3.0838744864331034 1107 90-91 3.0908926283184854 1107 92-93 2.9108864537303827 1107 94-95 2.788627731089308 1107 96-97 2.7326588628808413 1107 98-99 2.1084613225512356 1107 100-101 1.293847153081816 1108 1 0.25961998361443506 1108 2 0.21911489716741883 1108 3 0.06794151591368092 1108 4 0.1442020370939261 1108 5 0.16418491540977698 1108 6 0.15024733495325648 1108 7 0.23308725107090567 1108 8 0.275699021318907 1108 9 0.3155547041140352 1108 10-11 0.22265179503718002 1108 12-13 0.18958133384676756 1108 14-15 0.2328409252392305 1108 16-17 0.45850121008131595 1108 18-19 0.354159362965369 1108 20-21 0.42865958448324193 1108 22-23 0.44090029848127443 1108 24-25 0.4311017083292725 1108 26-27 0.2923829892613199 1108 28-29 0.45732012107480813 1108 30-31 0.5075713452826349 1108 32-33 0.5170946575858864 1108 34-35 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2.6114766738645674 2202 98-99 2.3969487200350255 2202 100-101 1.327425666543106 2203 1 0.2898905960569529 2203 2 0.3546949124236356 2203 3 0.31082295860703724 2203 4 0.31031389545856314 2203 5 0.4078942383726769 2203 6 0.33452100626390546 2203 7 0.28268616019244064 2203 8 0.39705630481110177 2203 9 0.46850781498570626 2203 10-11 0.46148416240911416 2203 12-13 0.4363674168631846 2203 14-15 0.5219425204878334 2203 16-17 0.664389054702184 2203 18-19 0.7079502921355569 2203 20-21 0.5986960978587774 2203 22-23 0.6168670945473806 2203 24-25 0.8324751751261985 2203 26-27 0.8215060929346265 2203 28-29 0.8347223601551903 2203 30-31 0.7270044548095953 2203 32-33 0.7159160610677802 2203 34-35 0.8383724138095872 2203 36-37 1.0839957336433699 2203 38-39 1.3295823115471634 2203 40-41 1.2962284801981383 2203 42-43 1.1457441450043717 2203 44-45 1.2426152737319853 2203 46-47 1.2821140350765958 2203 48-49 1.463095602757484 2203 50-51 1.4522779299878081 2203 52-53 1.4244895564984787 2203 54-55 1.5497724543433549 2203 56-57 1.5378043688294234 2203 58-59 1.5686186300182499 2203 60-61 1.645101691010968 2203 62-63 2.0211097938545315 2203 64-65 2.003657860231595 2203 66-67 2.383483780041754 2203 68-69 2.4537638542713545 2203 70-71 2.544610605487456 2203 72-73 2.562638261388649 2203 74-75 2.6820626483648304 2203 76-77 2.774122469503201 2203 78-79 2.8263390555896564 2203 80-81 2.81253592970387 2203 82-83 2.72974599078972 2203 84-85 2.7033745116938093 2203 86-87 2.8495417321209864 2203 88-89 2.8537094288054092 2203 90-91 2.8477907697721534 2203 92-93 2.7767528255247083 2203 94-95 2.8044443431762414 2203 96-97 2.7870371217163807 2203 98-99 2.6068711809274543 2203 100-101 1.7714199851321704 2204 1 0.3913048760473572 2204 2 0.44246088545100193 2204 3 0.3342015303665633 2204 4 0.3445424642800674 2204 5 0.3754651409913805 2204 6 0.39050309267794603 2204 7 0.4860646005842497 2204 8 0.5018349297923095 2204 9 0.5037854615436999 2204 10-11 0.5112411498846186 2204 12-13 0.5816960607240631 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-0.6278547762397046 2315 90-91 -0.5498262656907293 2315 92-93 -0.6379603204631668 2315 94-95 -0.6467916237493991 2315 96-97 -0.6185727039394333 2315 98-99 -0.6148439769879364 2315 100-101 -0.18311713263247054 2316 1 -1.1337877481469008 2316 2 -0.9135219207371641 2316 3 -0.7398041353917364 2316 4 -0.7581250138939453 2316 5 -0.6018686558591639 2316 6 -0.600365336410789 2316 7 -0.9211262913729144 2316 8 -0.8013218830830482 2316 9 -0.8803801423089865 2316 10-11 -0.9248946486840453 2316 12-13 -0.9053733587276653 2316 14-15 -1.1211717283841622 2316 16-17 -1.1207158736167457 2316 18-19 -1.132373470650002 2316 20-21 -1.1400721204690782 2316 22-23 -1.2590248194631144 2316 24-25 -1.02385156086725 2316 26-27 -1.2684798253000409 2316 28-29 -1.2027327089374253 2316 30-31 -1.1578757947935614 2316 32-33 -1.2947679596279187 2316 34-35 -1.2023474162104222 2316 36-37 -1.2919639690850744 2316 38-39 -1.5020001795853446 2316 40-41 -1.2642423093707365 2316 42-43 -1.182433171993786 2316 44-45 -1.3429621797580111 2316 46-47 -1.0187733113283315 2316 48-49 -0.8742290587781021 2316 50-51 -0.9849446616091733 2316 52-53 -0.8874277440791616 2316 54-55 -0.8470081442617996 2316 56-57 -0.7946466267855037 2316 58-59 -0.3837898213621145 2316 60-61 -0.6691736634202776 2316 62-63 -0.5882682257547849 2316 64-65 -0.7704321927280127 2316 66-67 -0.5607075093453808 2316 68-69 -0.6610483410860617 2316 70-71 -0.5601976544315477 2316 72-73 -0.4926695073039369 2316 74-75 -0.3676999416530471 2316 76-77 -0.4844006649134691 2316 78-79 -0.40925913896202815 2316 80-81 -0.466782119310448 2316 82-83 -0.35140769933441973 2316 84-85 -0.05636969887711629 2316 86-87 0.027999224902057307 2316 88-89 -0.31482747964570734 2316 90-91 -0.39292375848431327 2316 92-93 -0.5095310637786898 2316 94-95 -0.3453321035238037 2316 96-97 -0.44729709020328556 2316 98-99 -0.5130054734997298 2316 100-101 -0.21251602302146733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10404.0 3 3555.0 4 3794.0 5 4386.0 6 5441.0 7 7020.0 8 9179.0 9 11965.0 10 15318.0 11 19642.0 12 24803.0 13 29548.0 14 34786.0 15 40163.0 16 46461.0 17 53344.0 18 53479.0 19 54195.0 20 58835.0 21 65398.0 22 73074.0 23 81816.0 24 91185.0 25 101803.0 26 112333.0 27 123953.0 28 137525.0 29 152099.0 30 168527.0 31 186704.0 32 206274.0 33 230326.0 34 254162.0 35 269890.0 36 255282.0 37 169706.0 38 38007.0 39 460.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.902285771743585 18.91801813118089 14.709492949037417 23.470203148038106 2 15.255273626172979 19.97930656712179 40.63559738632267 24.129822420382563 3 19.105291118584308 24.241590937608663 29.645733463264566 27.007384480542463 4 12.673231906855042 16.49256720203956 37.26850254898098 33.56569834212441 5 13.540350446652285 37.71864668307092 34.3444088612966 14.396594008980191 6 32.30971869355124 36.276780869687926 17.370968235405037 14.042532201355801 7 28.724700318106212 32.70878470692932 20.564562824805606 18.001952150158864 8 26.513779710056255 34.907558308858164 19.755886136383996 18.82277584470158 9 26.619668631074266 16.64201792938121 20.01732382759826 36.720989611946266 10-11 24.78376546424777 26.589165229108957 27.64836680281911 20.978702503824167 12-13 25.994840340354635 23.936235708209402 27.66856950769431 22.400354443741655 14-15 22.60263155512817 25.49374040445561 25.976939169983655 25.926688870432567 16-17 22.022963516416024 28.570021903008808 27.010673113354468 22.3963414672207 18-19 22.064782066439367 27.085312627574748 29.193877410889623 21.656027895096262 20-21 22.625536459420108 26.34633581380381 29.383251338544188 21.644876388231893 22-23 22.251501816912864 26.336980684727386 29.27993630036463 22.131581197995125 24-25 22.068516658589633 26.53915098278382 29.373776251335443 22.018556107291104 26-27 21.695523185646078 26.863648558360183 29.134089054343647 22.306739201650096 28-29 21.817370577915902 27.25954426371909 29.02818282449997 21.894902333865037 30-31 21.984555671218082 26.799609209789104 28.840114677574668 22.375720441418142 32-33 21.53321100413462 26.807963237568444 28.91122851579142 22.747597242505517 34-35 22.479220933457203 26.918128004262325 28.64599874121594 21.95665232106453 36-37 22.51740306681014 26.373318704951203 28.711778994902176 22.397499233336482 38-39 21.944192096428946 26.88678730063423 28.681535625488983 22.48748497744784 40-41 22.447183606801406 26.634563158181347 28.816513656967945 22.1017395780493 42-43 22.16447029558754 26.666197522199454 28.568947618980005 22.600384563233003 44-45 22.10927039736917 26.919061490324353 28.76603212987135 22.205635982435126 46-47 22.61498336080265 27.116289622839655 28.004863021158915 22.263863995198776 48-49 22.25862158555065 27.190277593778422 27.93743720290606 22.613663617764864 50-51 22.485349745425644 27.29424695187171 27.90907628461711 22.31132701808554 52-53 22.7193819263126 27.00333932795908 28.000768909830033 22.27650983589829 54-55 22.099842148219654 27.246852442989173 28.251521217154714 22.401784191636462 56-57 22.265192206424434 27.427332124119907 27.91048700095567 22.39698866849999 58-59 22.171096276057643 26.924379088108108 28.037907812284494 22.866616823549748 60-61 21.839322029032452 27.23854084461158 28.31548566897614 22.60665145737983 62-63 22.019577416770233 27.19527408305462 28.436701064064668 22.34844743611048 64-65 21.769790218388003 27.199463145805186 28.230311198499532 22.80043543730728 66-67 21.784285418515832 27.269178520437798 28.05542714219361 22.89110891885276 68-69 22.42257023084609 26.820622308985143 28.14752393868153 22.609283521487242 70-71 22.16522135184851 26.856470307007367 27.864042781963555 23.114265559180566 72-73 22.136349884389087 26.981320166010374 27.89423992552969 22.988090024070846 74-75 22.162690731826967 27.376366705659116 27.616047788297987 22.844894774215927 76-77 22.330740868332235 26.94698552613185 27.80392189902742 22.918351706508496 78-79 22.32033637235126 27.142348490144 27.649815387150245 22.887499750354497 80-81 22.57368158918309 27.157381078395638 27.335726037178137 22.933211295243137 82-83 22.56694783590086 27.142960348631334 27.435182037041688 22.85490977842612 84-85 22.49399022615036 27.196693848147873 27.41776864707185 22.891547278629922 86-87 22.691444735729043 27.178912555177526 27.07853092086654 23.05111178822689 88-89 22.57315427528552 27.19983996624561 26.93508833688478 23.291917421584085 90-91 22.493165259873056 27.145844669948254 27.09444074511659 23.266549325062098 92-93 22.835363284870386 26.742918574134578 27.218467097933853 23.203251043061186 94-95 22.50940491995753 26.510919818961142 27.366678588615972 23.612996672465353 96-97 22.756765604828978 26.198017907831822 27.23108643062217 23.81413005671703 98-99 22.582824071227936 26.386725680010407 26.77518268994621 24.255267558815444 100-101 23.524911240764222 25.389788513323218 27.2096476825752 23.87565256333736 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 792.0 1 559.5 2 419.0 3 526.5 4 468.0 5 476.5 6 798.5 7 1705.0 8 2661.0 9 2864.5 10 2418.0 11 1998.0 12 2048.5 13 2177.0 14 2273.0 15 2467.0 16 2716.0 17 3053.5 18 3417.0 19 3869.5 20 4542.5 21 5509.5 22 6680.5 23 8134.0 24 10047.5 25 12738.5 26 16674.0 27 21268.5 28 25243.5 29 29982.5 30 36191.0 31 44313.0 32 55013.5 33 61836.5 34 65596.5 35 71701.5 36 79933.0 37 87373.0 38 91869.5 39 94927.5 40 98216.5 41 102641.5 42 105559.5 43 107940.5 44 110872.5 45 115112.5 46 117965.5 47 118910.0 48 119551.0 49 117797.0 50 114553.0 51 110126.0 52 106473.5 53 105158.5 54 103980.0 55 98622.0 56 90778.0 57 83374.5 58 76724.5 59 67991.5 60 56541.5 61 47064.5 62 39447.5 63 30681.0 64 22322.5 65 16688.5 66 12638.5 67 9676.0 68 7571.5 69 5949.0 70 4584.5 71 3339.5 72 2442.0 73 1899.5 74 1422.5 75 1059.0 76 788.0 77 541.0 78 351.0 79 221.5 80 135.0 81 83.0 82 51.0 83 29.0 84 20.0 85 15.5 86 11.0 87 7.5 88 4.5 89 3.0 90 3.5 91 4.0 92 3.5 93 1.5 94 1.0 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38819386415929397 2 0.028924982885271726 3 0.0341982537672684 4 0.043839914729025646 5 0.06287361436226808 6 0.053543981263350894 7 0.0372873296093848 8 0.03915949678642504 9 0.03625763766201267 10-11 0.03752135050651483 12-13 0.02959584279037781 14-15 0.027489654716207537 16-17 0.022341194979346876 18-19 0.026147934905995363 20-21 0.033745813366150346 22-23 0.032950142315908246 24-25 0.04101606257032328 26-27 0.035773994474610604 28-29 0.03099996817315799 30-31 0.03360540082787233 32-33 0.028675360594999692 34-35 0.02984546508064984 36-37 0.03196725454796212 38-39 0.01538297363801398 40-41 0.012964757701003669 42-43 0.013339191136411715 44-45 0.01181025460849552 46-47 0.020625041733726656 48-49 0.017894797933876303 50-51 0.011716646249643508 52-53 0.009516849816621226 54-55 0.007909906322995017 56-57 0.008721178766379123 58-59 0.003744334354080482 60-61 0.006895815768764888 62-63 0.011997471326199544 64-65 0.007722689605290994 66-67 0.004071963610062524 68-69 0.006115746111664788 70-71 0.012715135410731637 72-73 0.010718157088555379 74-75 0.011716646249643508 76-77 0.005304473668280683 78-79 0.009298430312633197 80-81 0.011794653215353518 82-83 0.013276785563843709 84-85 0.008440353689823087 86-87 0.0022622020055902913 88-89 0.01616304329511408 90-91 0.025165047138049238 92-93 0.010359325046289334 94-95 0.020843461237714685 96-97 0.018768475949828417 98-99 0.011513828138797482 100-101 0.12192488740474569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3204842.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.36850850917446 #Duplication Level Percentage of deduplicated Percentage of total 1 71.90578320125144 35.49881269829835 2 14.513222426589545 14.329922897252553 3 5.597635763601649 8.290407864798818 4 2.7107238498929314 5.352975737978453 5 1.5189117027455306 3.74932026608387 6 0.9093634115265432 2.6936349191928044 7 0.598077168200095 2.066832443720055 8 0.4154141322724055 1.6406700899137265 9 0.2961610735184148 1.3158927430272094 >10 1.3830358249030845 12.154536419231416 >50 0.09328048398070479 3.1623113998772516 >100 0.05184222597152411 4.8314129278732 >500 0.0031789978387181035 1.076216689188274 >1k 0.003178997837189888 3.1265433527878894 >5k 1.9073987023139328E-4 0.710509550776109 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9853 0.30744105325629156 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7295 0.2276243259418093 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5488 0.17124089112661403 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4392 0.13704263735934563 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4283 0.1336415336543892 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4234 0.13211259712647302 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3750 0.11701044856501507 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3504 0.10933456313915009 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3295 0.10281318080579323 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3270 0.10203311114869314 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.1202786284004015E-5 0.0 14-15 0.0 0.0 0.0 3.1202786284004015E-5 0.0 16-17 0.0 0.0 0.0 4.680417942600602E-5 0.0 18-19 0.0 0.0 0.0 9.360835885201205E-5 0.0 20-21 0.0 0.0 0.0 1.2481114513601606E-4 0.0 22-23 0.0 0.0 0.0 2.8082507655603615E-4 0.0 24-25 0.0 0.0 0.0 8.112724433841044E-4 0.0 26-27 0.0 0.0 0.0 0.0015757407073422028 0.0 28-29 0.0 0.0 0.0 0.0042279775414825445 0.0 30-31 0.0 0.0 0.0 0.01274633819701564 1.5601393142002008E-5 32-33 0.0 0.0 0.0 0.0318892475822521 3.1202786284004015E-5 34-35 0.0 0.0 0.0 0.06001855941728172 3.1202786284004015E-5 36-37 0.0 0.0 0.0 0.10811765447407393 3.1202786284004015E-5 38-39 0.0 0.0 0.0 0.17946282531244911 3.1202786284004015E-5 40-41 0.0 0.0 0.0 0.2579690356030032 3.1202786284004015E-5 42-43 0.0 0.0 0.0 0.3412804749812939 3.1202786284004015E-5 44-45 0.0 0.0 0.0 0.4466678856555175 3.1202786284004015E-5 46-47 0.0 0.0 0.0 0.5702621221264574 6.240557256800803E-5 48-49 0.0 0.0 0.0 0.7036852362768586 6.240557256800803E-5 50-51 0.0 0.0 0.0 0.8473428643284131 6.240557256800803E-5 52-53 0.0 0.0 0.0 0.9878334095721412 6.240557256800803E-5 54-55 0.0 0.0 0.0 1.1409298804746069 6.240557256800803E-5 56-57 0.0 0.0 0.0 1.3212975865892922 6.240557256800803E-5 58-59 0.0 0.0 0.0 1.5192480627750136 6.240557256800803E-5 60-61 0.0 0.0 0.0 1.7375895597973317 6.240557256800803E-5 62-63 0.0 0.0 0.0 1.9471630738738446 6.240557256800803E-5 64-65 0.0 0.0 0.0 2.170106981873053 6.240557256800803E-5 66-67 0.0 0.0 0.0 2.4297609679353926 6.240557256800803E-5 68-69 0.0 0.0 0.0 2.714158763520947 9.360835885201205E-5 70-71 0.0 0.0 0.0 3.008494646537957 9.360835885201205E-5 72-73 0.0 0.0 0.0 3.314547175804611 1.2481114513601606E-4 74-75 0.0 0.0 0.0 3.629430093589637 1.2481114513601606E-4 76-77 0.0 1.5601393142002008E-5 0.0 3.9634403193667582 1.2481114513601606E-4 78-79 0.0 3.1202786284004015E-5 0.0 4.331118351544319 1.560139314200201E-4 80-81 0.0 3.1202786284004015E-5 0.0 4.7214184037777835 1.560139314200201E-4 82-83 0.0 3.1202786284004015E-5 0.0 5.120829669606177 1.560139314200201E-4 84-85 0.0 3.1202786284004015E-5 0.0 5.530272631224878 1.560139314200201E-4 86-87 0.0 3.1202786284004015E-5 0.0 5.9489048134042175 1.560139314200201E-4 88-89 0.0 6.240557256800803E-5 0.0 6.379550068302899 1.560139314200201E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6175 0.0 44.02846 1 GTATCAA 10630 0.0 39.979744 1 ATCAACG 13710 0.0 30.646076 3 TATCAAC 13900 0.0 30.32975 2 TCAACGC 13880 0.0 30.304968 4 CAACGCA 14185 0.0 29.619394 5 AACGCAG 14745 0.0 28.550922 6 GTACATG 12230 0.0 27.183283 1 TACATGG 11980 0.0 27.097168 2 ACATGGG 12400 0.0 25.182783 3 ACGCAGA 16805 0.0 24.818682 7 CGCAGAG 16900 0.0 24.622952 8 GTGTAAG 1680 0.0 24.132662 1 TAAGGTG 2180 0.0 23.110142 5 GTAAGGT 2190 0.0 23.004976 4 GTATAGG 805 0.0 22.515589 1 GCAGAGT 18260 0.0 22.13798 9 AGGTAAG 2155 0.0 21.173079 2 GAGTACT 10080 0.0 20.876245 12-13 AGAGTAC 16035 0.0 20.54344 10-11 >>END_MODULE