FastQCFastQC Report
Thu 26 May 2016
SRR938432_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938432_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204842
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101020.31521054704100854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80880.25236813546502446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62420.19476779198475308No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA45500.14197267759221827No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA44890.14006930762889405No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC42240.13180056926363298No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39150.12215890830187572No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36730.11460783402114676No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG35770.11161236653788238No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66100.046.797531
GTATCAA111800.039.9511681
TAAGGTG21800.031.1573335
ATCAACG144250.030.6888663
TATCAAC146050.030.3478982
TCAACGC146650.030.154244
CAACGCA148750.029.6966025
GTAAGGT21600.028.3672164
AACGCAG157500.028.0467916
ACGCAGA178700.024.6928927
GGTAAGG23750.024.5992743
CGCAGAG179150.024.5513278
GTACATG127200.023.8329071
TACATGG123750.023.6858232
AGGTAAG22750.023.5963922
ACATGGG126450.022.350073
AAGGTGT32400.022.1366926
GGTGTGC32850.022.1226338
GAGTACT108350.021.56801612-13
GCAGAGT199650.021.5304579
Produced by FastQC (version 0.11.5)