##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938432_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3204842 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.799430673961464 31.0 31.0 34.0 30.0 34.0 2 32.05697691181032 33.0 31.0 34.0 30.0 34.0 3 32.11593395243822 33.0 31.0 34.0 30.0 34.0 4 35.77120182523819 37.0 35.0 37.0 35.0 37.0 5 35.66584218504376 37.0 35.0 37.0 33.0 37.0 6 35.69949002166097 37.0 35.0 37.0 33.0 37.0 7 35.62362387911791 37.0 35.0 37.0 33.0 37.0 8 35.59282329674911 37.0 35.0 37.0 33.0 37.0 9 37.14456438102097 39.0 37.0 39.0 33.0 39.0 10-11 37.06963042171814 39.0 37.0 39.0 33.0 39.0 12-13 36.955823095179106 39.0 37.0 39.0 33.0 39.0 14-15 37.95833133115455 40.0 37.0 41.0 33.0 41.0 16-17 38.04359403677311 40.0 37.0 41.0 33.0 41.0 18-19 38.19730769878827 40.0 37.5 41.0 33.0 41.0 20-21 38.15621518939155 40.0 37.0 41.0 33.0 41.0 22-23 38.02637353105083 40.0 37.0 41.0 33.0 41.0 24-25 37.899406741424386 40.0 37.0 41.0 32.5 41.0 26-27 37.677333235148566 40.0 37.0 41.0 32.0 41.0 28-29 37.53914436343508 39.5 36.5 41.0 32.0 41.0 30-31 37.27271297617792 39.0 36.0 41.0 31.0 41.0 32-33 37.151161586125 39.0 36.0 41.0 31.0 41.0 34-35 37.01468153500235 39.0 36.0 41.0 31.0 41.0 36-37 36.87840071367013 39.0 35.5 41.0 30.5 41.0 38-39 36.65339539359507 39.0 35.0 40.0 30.0 41.0 40-41 36.43006035866979 39.0 35.0 40.0 30.0 41.0 42-43 36.159648743994246 38.5 35.0 40.0 30.0 41.0 44-45 35.861086131547204 38.0 35.0 40.0 29.0 41.0 46-47 35.44420114938583 38.0 34.5 40.0 27.5 41.0 48-49 35.016270224866005 38.0 34.0 40.0 26.0 41.0 50-51 34.61494420005729 38.0 33.0 40.0 25.0 41.0 52-53 34.01493209337621 37.5 33.0 40.0 23.5 41.0 54-55 33.58352096608819 37.0 32.0 40.0 23.0 41.0 56-57 33.184823151968175 37.0 32.0 39.0 22.0 40.5 58-59 32.64055357487202 36.0 31.0 39.0 19.5 40.0 60-61 32.14001891512905 35.5 31.0 39.0 18.0 40.0 62-63 31.62483220701676 35.0 30.0 38.5 14.0 40.0 64-65 30.96450168214221 35.0 29.0 38.0 9.5 40.0 66-67 30.299442062978457 34.0 28.0 38.0 7.5 40.0 68-69 29.548277262966472 34.0 27.5 37.0 4.5 39.0 70-71 28.746902343391657 33.5 26.0 36.0 2.0 39.0 72-73 28.15785536385257 33.0 26.0 36.0 2.0 38.5 74-75 27.43501551714562 32.0 25.0 35.0 2.0 38.0 76-77 26.276974340700725 30.5 24.0 34.0 2.0 36.0 78-79 26.76542650152488 32.0 25.0 35.0 2.0 36.0 80-81 26.386326065372334 32.0 24.0 35.0 2.0 36.0 82-83 25.686912490537757 31.0 21.0 34.0 2.0 36.0 84-85 24.879081558466844 31.0 19.0 34.0 2.0 35.0 86-87 23.949750876954308 30.5 13.5 34.0 2.0 35.0 88-89 22.93044711720578 29.5 4.5 34.0 2.0 35.0 90-91 22.02733738511914 29.0 2.0 34.0 2.0 35.0 92-93 20.92110671914559 28.0 2.0 33.0 2.0 35.0 94-95 19.709346981848093 26.5 2.0 33.0 2.0 34.0 96-97 18.285690995063092 24.5 2.0 32.0 2.0 34.0 98-99 16.475527966745318 11.0 2.0 32.0 2.0 34.0 100-101 12.27506504220801 2.0 2.0 27.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29937822572909667 1101 2 0.2875975899092822 1101 3 0.29856721126640906 1101 4 0.17908787207314703 1101 5 0.10994594406432157 1101 6 0.1304675898688501 1101 7 0.22648117792643774 1101 8 0.23080086230391572 1101 9 0.228592910034898 1101 10-11 0.3171192307488795 1101 12-13 0.3386416596718078 1101 14-15 0.4869877979988644 1101 16-17 0.3523431605331808 1101 18-19 0.3766208063594192 1101 20-21 0.4151062263771479 1101 22-23 0.3774351835490961 1101 24-25 0.31141934326404197 1101 26-27 0.5099928326677841 1101 28-29 0.49009582090862835 1101 30-31 0.5418130360150997 1101 32-33 0.5967453993372658 1101 34-35 0.3158186296142915 1101 36-37 0.5072499350969935 1101 38-39 0.5080301148584923 1101 40-41 0.61599963228673 1101 42-43 0.6614163284106098 1101 44-45 0.7330884270313618 1101 46-47 0.7225171896838702 1101 48-49 0.8160149667183703 1101 50-51 0.8597016174136769 1101 52-53 0.958845295725034 1101 54-55 0.9778296522854859 1101 56-57 1.1008690429597507 1101 58-59 1.140075420368646 1101 60-61 1.1492382917473165 1101 62-63 1.1228435646269403 1101 64-65 1.2071778832536886 1101 66-67 1.319672577232101 1101 68-69 1.4162332563943743 1101 70-71 1.592888112896727 1101 72-73 1.7785798067785237 1101 74-75 2.0288037227965283 1101 76-77 1.8432741556245844 1101 78-79 1.7826101431953703 1101 80-81 1.9129541723335777 1101 82-83 2.096407891582057 1101 84-85 2.3730038704063006 1101 86-87 2.333140464094754 1101 88-89 2.279897876508702 1101 90-91 2.442827502518192 1101 92-93 2.629160602635082 1101 94-95 2.453919479720863 1101 96-97 2.4983426819479 1101 98-99 2.7718738628005646 1101 100-101 2.4094401781180643 1102 1 0.257977160798621 1102 2 0.30147701555341655 1102 3 0.22315650644394225 1102 4 0.22539215955390546 1102 5 0.2339687063223792 1102 6 0.2604193904088845 1102 7 0.2666431886766816 1102 8 0.29532252682557925 1102 9 0.33537111681309995 1102 10-11 0.3239100551867651 1102 12-13 0.4270214259927485 1102 14-15 0.515724517912048 1102 16-17 0.4232600858618696 1102 18-19 0.4515413151034551 1102 20-21 0.38204669625879006 1102 22-23 0.46585469549802383 1102 24-25 0.40708454628218504 1102 26-27 0.3650853201132165 1102 28-29 0.4109584903007075 1102 30-31 0.4185435480397288 1102 32-33 0.444979557865544 1102 34-35 0.6062500273986373 1102 36-37 0.5675541468717924 1102 38-39 0.6902190264591681 1102 40-41 0.7055130953296 1102 42-43 0.7022508604216142 1102 44-45 0.6634660735854823 1102 46-47 0.5861625577409981 1102 48-49 0.7127192340108763 1102 50-51 0.859134453611226 1102 52-53 0.746785967489231 1102 54-55 0.8151886499385981 1102 56-57 0.9774878886962455 1102 58-59 1.1622768234524088 1102 60-61 1.1756716578865607 1102 62-63 1.278845154452057 1102 64-65 1.3585740272968927 1102 66-67 1.4320139101616718 1102 68-69 1.5412540755328408 1102 70-71 1.60094133418524 1102 72-73 1.8434932496331413 1102 74-75 1.6613667480066105 1102 76-77 1.6905026866766448 1102 78-79 1.894095367915888 1102 80-81 2.1204036386065717 1102 82-83 2.1854961394938357 1102 84-85 2.249030317020985 1102 86-87 2.2869610914447946 1102 88-89 2.203286601368017 1102 90-91 2.5495154930297126 1102 92-93 2.8543637469558725 1102 94-95 2.694398824611973 1102 96-97 2.8327447310558327 1102 98-99 2.7535441885885383 1102 100-101 2.4152228515477976 1103 1 0.24636760481406483 1103 2 0.20566540036679726 1103 3 0.23655740734484 1103 4 0.15489465311890172 1103 5 0.14510730027347307 1103 6 0.21734667483617187 1103 7 0.2458255406715324 1103 8 0.26357864008168974 1103 9 0.35925263225711745 1103 10-11 0.35911677976849177 1103 12-13 0.33076277895285244 1103 14-15 0.43237791237794454 1103 16-17 0.3800994922325245 1103 18-19 0.3632966642025579 1103 20-21 0.27918915121374965 1103 22-23 0.4504110822419136 1103 24-25 0.38761623868887085 1103 26-27 0.5030126447592878 1103 28-29 0.3803286640458836 1103 30-31 0.37928035627653855 1103 32-33 0.4568863307098212 1103 34-35 0.4860953861502466 1103 36-37 0.4715830641819636 1103 38-39 0.573928807668949 1103 40-41 0.6720484476462047 1103 42-43 0.5405043594563637 1103 44-45 0.6967597914104502 1103 46-47 0.6260547713832167 1103 48-49 0.8115746072412477 1103 50-51 0.8652173445378608 1103 52-53 1.0352954591237236 1103 54-55 1.0841685003247008 1103 56-57 1.2370434710643323 1103 58-59 1.2132412700418485 1103 60-61 1.1619445825969876 1103 62-63 1.3176965058034114 1103 64-65 1.5130469600510743 1103 66-67 1.5012218010883096 1103 68-69 1.3431021289433964 1103 70-71 1.6979800246614296 1103 72-73 1.6232356482817885 1103 74-75 1.631217938482802 1103 76-77 1.7448111245788311 1103 78-79 1.7501326105156316 1103 80-81 1.976505030176714 1103 82-83 1.9982052772029704 1103 84-85 2.1680079553736213 1103 86-87 2.208599001676454 1103 88-89 2.113328227815895 1103 90-91 2.286135304805775 1103 92-93 2.644449614072993 1103 94-95 2.51292581142021 1103 96-97 2.422594111686461 1103 98-99 2.343933707571807 1103 100-101 2.157774532693228 1104 1 0.1861292735144886 1104 2 0.2212202356322095 1104 3 0.23067729752177968 1104 4 0.19211151553501082 1104 5 0.1579991169299646 1104 6 0.11180823676417617 1104 7 0.12824755867031712 1104 8 0.12120829230865837 1104 9 0.1638108613602327 1104 10-11 0.21909885725475675 1104 12-13 0.24703461009989525 1104 14-15 0.27680115063297706 1104 16-17 0.25679669708304687 1104 18-19 0.3212322384856563 1104 20-21 0.27598883852308376 1104 22-23 0.24132646509731615 1104 24-25 0.2576669555894213 1104 26-27 0.38169295668728864 1104 28-29 0.2788006744180578 1104 30-31 0.33141733004102747 1104 32-33 0.4174119942241532 1104 34-35 0.4200046867908753 1104 36-37 0.4762462543894088 1104 38-39 0.5027741401887837 1104 40-41 0.6131988251629892 1104 42-43 0.7420879279432455 1104 44-45 0.7301317979693209 1104 46-47 0.7247590117736991 1104 48-49 0.7210167213352037 1104 50-51 0.8874161891077179 1104 52-53 0.8346681711432495 1104 54-55 0.9503520535583831 1104 56-57 1.0032665872937372 1104 58-59 1.1283903701028635 1104 60-61 1.1170080740551995 1104 62-63 1.253899983533735 1104 64-65 1.3622556048039733 1104 66-67 1.421247445536146 1104 68-69 1.4115499248555352 1104 70-71 1.4515761579744257 1104 72-73 1.6021937591725752 1104 74-75 1.6788716145047644 1104 76-77 1.7387617995330729 1104 78-79 1.7174608213149014 1104 80-81 1.8753813457654864 1104 82-83 1.954840420583082 1104 84-85 2.1903115703827716 1104 86-87 2.2790261866978057 1104 88-89 2.1628644351069255 1104 90-91 2.502154886105348 1104 92-93 2.5885491984232054 1104 94-95 2.369050258491594 1104 96-97 2.71627347028927 1104 98-99 2.5968105570226925 1104 100-101 2.141746562131912 1105 1 0.20199260481406256 1105 2 0.21471824315107568 1105 3 0.19718972057616924 1105 4 0.1703522926328489 1105 5 0.13080008830227285 1105 6 0.08092528269695975 1105 7 0.08720989785724953 1105 8 0.09984776699438669 1105 9 0.22705275601949637 1105 10-11 0.2328227128617968 1105 12-13 0.21359989078903396 1105 14-15 0.3362705203887444 1105 16-17 0.3164669258508894 1105 18-19 0.2941897691380504 1105 20-21 0.27699565061368503 1105 22-23 0.3238332057042612 1105 24-25 0.29346063562856983 1105 26-27 0.27677759937974855 1105 28-29 0.3020378974692264 1105 30-31 0.39089676791769534 1105 32-33 0.47453700202694904 1105 34-35 0.49635477146377127 1105 36-37 0.5383071584663952 1105 38-39 0.49464362915109916 1105 40-41 0.4474186565420908 1105 42-43 0.44524750564598037 1105 44-45 0.6567935916654761 1105 46-47 0.6258623771437897 1105 48-49 0.7453780500855345 1105 50-51 0.912714859914395 1105 52-53 0.7263442515029581 1105 54-55 0.6590490977594428 1105 56-57 0.8359585813253503 1105 58-59 0.9497753609509445 1105 60-61 1.0152485692456494 1105 62-63 1.0615646426170926 1105 64-65 1.228846961926262 1105 66-67 1.2798487419073936 1105 68-69 1.3802556880493064 1105 70-71 1.452533636272591 1105 72-73 1.6427194560375646 1105 74-75 1.7625090050894592 1105 76-77 1.7835578898803632 1105 78-79 1.862205161520734 1105 80-81 2.0108111639150863 1105 82-83 2.2178947461498666 1105 84-85 2.295198736951779 1105 86-87 2.463281860587344 1105 88-89 2.4073672410922207 1105 90-91 2.3832312487751715 1105 92-93 2.6032315391404985 1105 94-95 2.5124776884829174 1105 96-97 2.7555295771080033 1105 98-99 2.760001204946544 1105 100-101 2.4466908149464555 1106 1 0.04188446423130543 1106 2 0.06275480409163947 1106 3 0.017644070006305412 1106 4 0.0666591657353024 1106 5 0.008003909615617033 1106 6 -0.016658281607206504 1106 7 0.11360649966822933 1106 8 -0.01617353774948782 1106 9 -0.16630712667949865 1106 10-11 0.09156026880423696 1106 12-13 0.04740417839713018 1106 14-15 -0.018579488493188023 1106 16-17 -0.10244345422286472 1106 18-19 0.12641391975977712 1106 20-21 0.0936853400091806 1106 22-23 0.2242191890307339 1106 24-25 0.14950625821425945 1106 26-27 -0.14111120061470217 1106 28-29 0.19460904103596732 1106 30-31 0.2539656972197619 1106 32-33 0.14681902446169204 1106 34-35 0.14478535498472667 1106 36-37 0.17775256891311386 1106 38-39 0.2803428959837575 1106 40-41 0.3121102623803651 1106 42-43 0.3159221369420919 1106 44-45 0.3663466136543647 1106 46-47 -0.04917390089074303 1106 48-49 0.22213915365158243 1106 50-51 0.5653584811404428 1106 52-53 0.4224847426923617 1106 54-55 0.35850055386931245 1106 56-57 0.38795750305592236 1106 58-59 0.716330974157195 1106 60-61 0.7629684448679441 1106 62-63 0.7487756587021792 1106 64-65 0.9112591202343125 1106 66-67 0.8296617886972051 1106 68-69 0.849208035371749 1106 70-71 0.7154187262234579 1106 72-73 1.0780160346590826 1106 74-75 0.9789832208498801 1106 76-77 1.0063336820285187 1106 78-79 1.3573694872053288 1106 80-81 1.3703772046285003 1106 82-83 1.6542356166442467 1106 84-85 1.7102276534811587 1106 86-87 1.6870921208563843 1106 88-89 1.747352381090149 1106 90-91 1.9203558383744124 1106 92-93 1.7423204555419005 1106 94-95 1.64730048286809 1106 96-97 1.7103714566098631 1106 98-99 1.6728729066556163 1106 100-101 1.7030444617259715 1107 1 0.06284974767120488 1107 2 0.07857611465251324 1107 3 0.03862883591627053 1107 4 0.07638572454747106 1107 5 0.025634085987753963 1107 6 0.01913238912188575 1107 7 -0.015076245042749292 1107 8 0.04000721151026454 1107 9 0.07824370368568623 1107 10-11 0.111063208339921 1107 12-13 0.008981528952851647 1107 14-15 0.15761898380651473 1107 16-17 0.11677359937537801 1107 18-19 0.1537832713454108 1107 20-21 0.08614450835660392 1107 22-23 0.05231286795618928 1107 24-25 0.10763409583173456 1107 26-27 0.1820171090449989 1107 28-29 0.19184652118874368 1107 30-31 0.21720892770510858 1107 32-33 0.3132524021383887 1107 34-35 0.27071592186452875 1107 36-37 0.23543574275339552 1107 38-39 0.2594288076689466 1107 40-41 0.31754398336048695 1107 42-43 0.3617141808849311 1107 44-45 0.4167196128390174 1107 46-47 0.6313761999546443 1107 48-49 0.519521035812673 1107 50-51 0.6376325231092892 1107 52-53 0.6857061734094358 1107 54-55 0.6094318931818421 1107 56-57 0.5581416853500443 1107 58-59 0.755437698613278 1107 60-61 0.7712392254541314 1107 62-63 0.9459107915176972 1107 64-65 1.0341853529082137 1107 66-67 0.9850834082311692 1107 68-69 1.2435217718005376 1107 70-71 1.1915603818042868 1107 72-73 1.452284755424646 1107 74-75 1.4905572241970866 1107 76-77 1.5241191602931181 1107 78-79 1.6784093962299167 1107 80-81 1.6905452087481443 1107 82-83 1.8114909914886894 1107 84-85 2.1029543839450504 1107 86-87 2.3787641802478845 1107 88-89 2.3477344778158944 1107 90-91 2.39857726909149 1107 92-93 2.4266237212158472 1107 94-95 2.0936356328487804 1107 96-97 2.206375361686465 1107 98-99 2.166098886143235 1107 100-101 1.9052254255503716 1108 1 0.013110183393806096 1108 2 0.04542893354491184 1108 3 -0.005162615938047566 1108 4 0.07896879456244221 1108 5 0.06103970714192286 1108 6 -0.002010135408298197 1108 7 0.02928996150971841 1108 8 0.02441100329473045 1108 9 0.04027962585432476 1108 10-11 0.06391669245765996 1108 12-13 0.053264416030849304 1108 14-15 0.1296316230880734 1108 16-17 0.11149919392053675 1108 18-19 0.003492567869606944 1108 20-21 0.0625546197818494 1108 22-23 0.044245846502676045 1108 24-25 0.10836783938736261 1108 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-0.6181791586974725 2316 64-65 -0.46708880117160945 2316 66-67 -0.46552666686798005 2316 68-69 -0.2192012895444968 2316 70-71 -0.2629238811271115 2316 72-73 0.0242594411051833 2316 74-75 0.33574725035521524 2316 76-77 0.4102270836751103 2316 78-79 0.1441522871252019 2316 80-81 -0.42977164215051644 2316 82-83 0.3045992523949401 2316 84-85 0.47435873800799655 2316 86-87 0.5184420133421348 2316 88-89 0.7572300478099869 2316 90-91 1.0976323699268633 2316 92-93 1.0521808050062482 2316 94-95 1.2579199970374546 2316 96-97 0.9518573463291098 2316 98-99 0.9562250358352458 2316 100-101 0.48687330336658974 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 17.0 4 50.0 5 89.0 6 254.0 7 598.0 8 1493.0 9 2782.0 10 4580.0 11 7046.0 12 10337.0 13 14226.0 14 19431.0 15 25504.0 16 32395.0 17 40356.0 18 40498.0 19 38334.0 20 39952.0 21 43356.0 22 47533.0 23 53300.0 24 60775.0 25 69144.0 26 79210.0 27 92662.0 28 108990.0 29 128012.0 30 148619.0 31 174298.0 32 203943.0 33 242361.0 34 292756.0 35 352051.0 36 395355.0 37 333354.0 38 99432.0 39 1743.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.96104207396088 19.759262588356314 15.244507614061106 23.035187723621693 2 15.79051334677909 20.76901064478148 39.62557431296987 23.814901695469562 3 19.149369035070805 24.50500409701277 30.19776121498739 26.147865652929035 4 13.186453497551517 17.264595259298275 36.869867531691106 32.6790837114591 5 13.456401398010945 37.841370502543185 34.42503663990609 14.277191459539775 6 32.15138218982403 36.88106933196706 16.993723871566836 13.973824606642074 7 28.64284729169176 32.83818671872123 20.497235121107373 18.021730868479633 8 26.467430324849595 35.419024430883695 19.501428621798862 18.612116622467852 9 26.37565627424073 16.706543927080155 19.885335714735927 37.032464083943196 10-11 24.808976542369326 26.823147599788072 27.513665260253077 20.854210597589525 12-13 25.943385040510574 24.039000986632104 27.705250368036864 22.312363604820458 14-15 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26.868882903445563 28.842191196530067 22.347860360044074 40-41 22.393059517932272 26.599590069547123 29.001549721855195 22.005800690665406 42-43 22.181457175586072 26.691035201965622 28.696446664392912 22.431060958055394 44-45 22.134238428367272 26.889450889597544 28.87305683314508 22.10325384889011 46-47 22.597859050017803 27.094918028643562 28.125406611689385 22.181816309649253 48-49 22.221579098127147 27.23734898631508 28.026202227754133 22.514869687803643 50-51 22.483417279229368 27.279332335260207 28.021272187521255 22.215978197989166 52-53 22.811233751929112 27.00902259768188 27.988883695358464 22.190859955030547 54-55 22.140467455181877 27.27794381127057 28.220205551474926 22.361383182072625 56-57 22.324439083112367 27.4363759586276 27.90705438832866 22.332130569931373 58-59 22.191811640012208 27.01056713560294 27.979070419072144 22.81855080531271 60-61 21.84530469832834 27.321050460521924 28.246805926782038 22.586838914367696 62-63 22.025922026733298 27.303155662588047 28.338136482235317 22.332785828443335 64-65 21.716672550996705 27.288605248406355 28.174733867327834 22.81998833326911 66-67 21.748338014553987 27.393495404509416 28.016836480851616 22.84133010008498 68-69 22.381823985581704 26.928557855253516 28.037357515265306 22.65226064389948 70-71 22.11373011738646 26.999307142022467 27.816071403219162 23.07089133737191 72-73 22.019941113991482 27.142917765526846 27.794838799863708 23.042302320617967 74-75 22.069091081557218 27.488547017294458 27.504741263375855 22.937620637772472 76-77 22.064785097050027 27.063611872285747 28.01676338490322 22.854839645761007 78-79 22.079247588492663 27.268380157274525 27.857613573461652 22.794758680771157 80-81 22.277725114342754 27.313143441259747 27.62167697712022 22.787454467277286 82-83 22.302954848443008 27.342024935897285 27.628484305268554 22.726535910391156 84-85 22.08167754920546 27.45936795462878 27.629463206097345 22.829491290068415 86-87 22.302759340207228 27.449099901754703 27.310055578724405 22.93808517931366 88-89 22.067827368712717 27.525990360835262 27.082583166346424 23.3235991041056 90-91 21.923433118884574 27.3922196850605 27.497592677996735 23.18675451805819 92-93 22.167383162770594 27.116034925382216 27.56331259121967 23.15326932062752 94-95 21.829254270453 26.871016866200716 27.704661837410683 23.595067025935602 96-97 22.03723647487715 26.56630600920514 27.692867182355197 23.70359033356252 98-99 21.703363223522405 26.884866711057832 27.345263822678312 24.06650624274145 100-101 21.935416432645624 26.412129778709705 27.806146328677873 23.846307459966802 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 884.0 1 618.5 2 441.5 3 542.0 4 487.5 5 495.5 6 890.0 7 1935.5 8 2967.0 9 3209.0 10 2661.5 11 2125.5 12 2096.5 13 2236.0 14 2374.0 15 2579.5 16 2902.0 17 3175.0 18 3542.5 19 4044.0 20 4728.0 21 5573.5 22 6684.0 23 8326.5 24 10346.0 25 12853.5 26 16570.5 27 21154.0 28 25308.5 29 30262.0 30 36232.5 31 44442.5 32 56930.0 33 63623.5 34 66155.5 35 72244.5 36 80698.5 37 88834.0 38 92999.5 39 95826.5 40 99371.5 41 104097.5 42 107105.5 43 109175.0 44 112513.0 45 117744.0 46 119871.0 47 120395.5 48 121677.0 49 119573.5 50 115624.5 51 110628.0 52 106164.5 53 105165.5 54 105001.0 55 98586.5 56 89763.5 57 81515.5 58 74471.0 59 65755.0 60 53343.0 61 44256.0 62 37475.5 63 28015.5 64 19220.0 65 14116.5 66 10658.5 67 8292.0 68 6494.5 69 5143.0 70 4003.0 71 2693.5 72 1946.5 73 1620.5 74 1199.5 75 871.5 76 640.0 77 426.0 78 272.0 79 163.0 80 101.0 81 58.5 82 30.0 83 18.0 84 14.5 85 8.5 86 1.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024026145438683092 2 0.01126420584852545 3 0.002121789467312273 4 0.0 5 9.360835885201205E-5 6 0.0 7 0.0 8 1.560139314200201E-4 9 2.496222902720321E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001856565783898239 26-27 0.008955199663509153 28-29 3.2762925598204217E-4 30-31 0.0 32-33 2.964264696980382E-4 34-35 0.010187709721727311 36-37 0.0011857058787921528 38-39 0.012512317299885611 40-41 0.01235630336846559 42-43 0.005086054164292655 44-45 6.864612982480884E-4 46-47 9.360835885201205E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.800696571001004E-5 66-67 4.836431874020623E-4 68-69 7.800696571001003E-4 70-71 1.5601393142002008E-5 72-73 1.872167177040241E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2481114513601606E-4 82-83 7.644682639580985E-4 84-85 0.005210865309428671 86-87 0.0033855023118144357 88-89 0.0 90-91 0.0011233003062241446 92-93 2.964264696980382E-4 94-95 1.5601393142002008E-5 96-97 1.5601393142002008E-5 98-99 0.0 100-101 6.240557256800803E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3204842.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.49276444440573 #Duplication Level Percentage of deduplicated Percentage of total 1 69.14104363876896 32.14558255337662 2 15.672275671022678 14.572948421612963 3 6.204916428117479 8.654511536690679 4 3.0731372400323522 5.715145832246211 5 1.683965981253337 3.9146116849401973 6 1.0361879505745601 2.8905145383716726 7 0.6726688224164775 2.1891963176793547 8 0.45732895590112155 1.7009989936253485 9 0.3306288732011118 1.383466528823272 >10 1.5532178478110301 12.870639111697987 >50 0.10588293193685434 3.38722260417208 >100 0.06150093539162989 5.377543519804768 >500 0.003555281013143157 1.1504209172092166 >1k 0.003488200237883348 3.2852194030229738 >5k 1.3416154761089803E-4 0.44691982917036954 >10k+ 6.708077380544901E-5 0.3150582075561112 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10102 0.31521054704100854 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8088 0.25236813546502446 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6242 0.19476779198475308 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4550 0.14197267759221827 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4489 0.14006930762889405 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4224 0.13180056926363298 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3915 0.12215890830187572 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3673 0.11460783402114676 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3577 0.11161236653788238 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.1202786284004015E-5 0.0 4 0.0 0.0 0.0 3.1202786284004015E-5 0.0 5 0.0 0.0 0.0 3.1202786284004015E-5 0.0 6 0.0 0.0 0.0 3.1202786284004015E-5 0.0 7 0.0 0.0 0.0 3.1202786284004015E-5 0.0 8 0.0 0.0 0.0 3.1202786284004015E-5 0.0 9 0.0 0.0 0.0 3.1202786284004015E-5 0.0 10-11 0.0 0.0 0.0 3.1202786284004015E-5 0.0 12-13 0.0 0.0 0.0 7.800696571001004E-5 0.0 14-15 0.0 0.0 0.0 9.360835885201205E-5 0.0 16-17 0.0 0.0 0.0 1.0920975199401405E-4 0.0 18-19 0.0 0.0 0.0 1.560139314200201E-4 0.0 20-21 0.0 0.0 0.0 2.184195039880281E-4 0.0 22-23 0.0 0.0 0.0 3.7443343540804824E-4 0.0 24-25 0.0 0.0 0.0 9.048808022361165E-4 0.0 26-27 0.0 0.0 0.0 0.0016225448867682088 0.0 28-29 0.0 0.0 0.0 0.004259180327766548 0.0 30-31 0.0 0.0 0.0 0.012808743769583648 0.0 32-33 0.0 0.0 0.0 0.03223247823137615 0.0 34-35 0.0 0.0 0.0 0.06082983186066583 0.0 36-37 0.0 0.0 0.0 0.10925655617344006 0.0 38-39 0.0 0.0 0.0 0.18069533537066726 0.0 40-41 0.0 0.0 0.0 0.2595135735240614 0.0 42-43 0.0 0.0 0.0 0.34313704076519214 0.0 44-45 0.0 0.0 0.0 0.44878967512282975 0.0 46-47 0.0 0.0 0.0 0.5727739464223197 0.0 48-49 0.0 0.0 0.0 0.7064310814698509 0.0 50-51 3.1202786284004015E-5 0.0 0.0 0.8500731081282634 0.0 52-53 3.1202786284004015E-5 0.0 0.0 0.9906572617308436 0.0 54-55 3.1202786284004015E-5 0.0 0.0 1.1439565507441554 0.0 56-57 3.1202786284004015E-5 0.0 0.0 1.3244334666108344 0.0 58-59 3.1202786284004015E-5 0.0 0.0 1.522789579018248 0.0 60-61 3.1202786284004015E-5 0.0 0.0 1.74014818827262 3.1202786284004015E-5 62-63 3.1202786284004015E-5 0.0 0.0 1.949331667520583 3.1202786284004015E-5 64-65 3.1202786284004015E-5 0.0 0.0 2.173071246570034 3.1202786284004015E-5 66-67 3.1202786284004015E-5 0.0 0.0 2.4335365050757574 3.1202786284004015E-5 68-69 3.1202786284004015E-5 0.0 0.0 2.7195568455480803 3.1202786284004015E-5 70-71 3.1202786284004015E-5 0.0 0.0 3.0191503980539447 3.1202786284004015E-5 72-73 3.1202786284004015E-5 0.0 0.0 3.333112833643593 3.1202786284004015E-5 74-75 3.1202786284004015E-5 0.0 0.0 3.658386279261193 3.1202786284004015E-5 76-77 3.1202786284004015E-5 0.0 0.0 4.00498682930391 3.1202786284004015E-5 78-79 3.1202786284004015E-5 0.0 0.0 4.387532989145798 3.1202786284004015E-5 80-81 3.1202786284004015E-5 0.0 0.0 4.796492307577097 3.1202786284004015E-5 82-83 3.1202786284004015E-5 0.0 0.0 5.218525593461393 3.1202786284004015E-5 84-85 3.1202786284004015E-5 0.0 0.0 5.653352021722132 3.1202786284004015E-5 86-87 3.1202786284004015E-5 0.0 0.0 6.102360116348949 3.1202786284004015E-5 88-89 3.1202786284004015E-5 0.0 0.0 6.573709405955114 3.1202786284004015E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6610 0.0 46.79753 1 GTATCAA 11180 0.0 39.951168 1 TAAGGTG 2180 0.0 31.157333 5 ATCAACG 14425 0.0 30.688866 3 TATCAAC 14605 0.0 30.347898 2 TCAACGC 14665 0.0 30.15424 4 CAACGCA 14875 0.0 29.696602 5 GTAAGGT 2160 0.0 28.367216 4 AACGCAG 15750 0.0 28.046791 6 ACGCAGA 17870 0.0 24.692892 7 GGTAAGG 2375 0.0 24.599274 3 CGCAGAG 17915 0.0 24.551327 8 GTACATG 12720 0.0 23.832907 1 TACATGG 12375 0.0 23.685823 2 AGGTAAG 2275 0.0 23.596392 2 ACATGGG 12645 0.0 22.35007 3 AAGGTGT 3240 0.0 22.136692 6 GGTGTGC 3285 0.0 22.122633 8 GAGTACT 10835 0.0 21.568016 12-13 GCAGAGT 19965 0.0 21.530457 9 >>END_MODULE