FastQCFastQC Report
Thu 26 May 2016
SRR938431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308492
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73960.22354595386659543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59490.17981001616446404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.1270971790169056No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36790.11119869717079564No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35320.10675558532406909No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.051.5037771
GTATCAA87550.046.118421
ATCAACG112650.035.2926943
TCAACGC114000.034.9581834
TATCAAC114050.034.9011652
CAACGCA117100.034.0733455
AACGCAG121650.032.969936
ACGCAGA139950.028.3522877
CGCAGAG142050.027.8662368
GTACATG116650.025.7257181
GCAGAGT156200.024.397669
TACATGG118450.024.330292
GTAAGGT17500.024.1858674
TAAGGTG19700.023.8989435
ACATGGG120300.023.0469073
GGACCGA11800.022.1497276
GAGTACT86050.022.1419312-13
TGGTATC24750.021.3283622
GTGGTAT27750.021.251111
AGAGTAC142100.021.08381310-11
Produced by FastQC (version 0.11.5)