##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938431_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3308492 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.004503260095536 31.0 31.0 34.0 28.0 34.0 2 31.038322293056776 31.0 31.0 34.0 27.0 34.0 3 31.084927211551367 31.0 31.0 34.0 27.0 34.0 4 34.736442161564845 37.0 35.0 37.0 32.0 37.0 5 34.71072077550739 37.0 35.0 37.0 32.0 37.0 6 34.7191917042568 37.0 35.0 37.0 31.0 37.0 7 34.62893850128699 37.0 35.0 37.0 30.0 37.0 8 34.58829611798971 37.0 35.0 37.0 30.0 37.0 9 36.06214371985787 39.0 35.0 39.0 30.0 39.0 10-11 35.887526704008955 38.0 35.0 39.0 30.0 39.0 12-13 35.73526745719802 38.0 35.0 39.0 30.0 39.0 14-15 36.67359782039672 39.0 36.0 41.0 30.0 41.0 16-17 36.48854463000062 39.0 36.0 40.5 30.0 41.0 18-19 36.63277151644918 39.0 36.0 41.0 30.0 41.0 20-21 36.60233665367787 39.0 36.0 41.0 30.0 41.0 22-23 36.50971409330897 39.0 36.0 41.0 29.5 41.0 24-25 36.35958754018447 39.0 35.5 41.0 29.0 41.0 26-27 36.13497508834841 39.0 35.0 40.5 28.0 41.0 28-29 35.9170396966352 39.0 35.0 40.0 27.0 41.0 30-31 35.662341332546674 38.0 34.5 40.0 27.0 41.0 32-33 35.381254964497415 38.0 34.0 40.0 26.5 41.0 34-35 35.1231007963749 38.0 34.0 40.0 25.0 41.0 36-37 34.83182957673768 38.0 34.0 40.0 25.0 41.0 38-39 34.68719026674388 38.0 33.0 40.0 24.0 41.0 40-41 34.57728445466998 38.0 33.0 40.0 24.0 41.0 42-43 34.27164626663749 38.0 33.0 40.0 23.5 41.0 44-45 33.92298198091457 38.0 33.0 40.0 22.5 41.0 46-47 33.531749510048684 38.0 32.5 40.0 20.0 41.0 48-49 33.15935281088787 37.5 31.5 40.0 18.0 41.0 50-51 32.47005901782444 36.5 30.5 39.5 15.5 40.5 52-53 32.484958706262546 37.0 31.0 39.0 15.0 40.0 54-55 32.5646326181233 37.0 31.0 40.0 13.5 41.0 56-57 32.21885408820695 36.5 31.0 39.5 10.5 41.0 58-59 31.8182430243144 36.0 30.5 39.0 8.0 41.0 60-61 31.310061502340037 35.5 29.5 39.0 6.5 40.0 62-63 30.709701882307712 35.0 28.5 38.5 2.0 40.0 64-65 30.160550637571436 35.0 28.0 38.0 2.0 40.0 66-67 29.583038435637746 34.0 28.0 38.0 2.0 40.0 68-69 28.95762344294621 34.0 26.5 37.0 2.0 39.5 70-71 28.285826594109945 34.0 26.0 36.5 2.0 39.0 72-73 27.617231657202133 33.0 25.0 36.0 2.0 39.0 74-75 26.903789400125497 32.5 23.5 35.5 2.0 38.0 76-77 26.120206728624403 32.0 21.0 35.0 2.0 37.0 78-79 25.297404376374494 31.0 19.0 35.0 2.0 36.5 80-81 24.565688083876278 31.0 15.0 34.5 2.0 36.0 82-83 23.79177809709076 30.5 7.5 34.0 2.0 35.5 84-85 23.059675979267897 30.0 4.0 34.0 2.0 35.0 86-87 22.259416525716247 29.0 2.0 34.0 2.0 35.0 88-89 21.403611524525374 29.0 2.0 34.0 2.0 35.0 90-91 20.45090663661874 27.0 2.0 33.5 2.0 35.0 92-93 19.464187762884116 26.0 2.0 33.0 2.0 35.0 94-95 18.422315363011307 24.5 2.0 32.5 2.0 34.0 96-97 17.14934492814249 20.5 2.0 32.0 2.0 34.0 98-99 15.616736416470102 2.0 2.0 32.0 2.0 34.0 100-101 12.24626733266999 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4630839656880781 1101 2 0.49995171195978116 1101 3 0.4962036649361181 1101 4 0.43771914905310894 1101 5 0.3263046013734652 1101 6 0.5241921268264562 1101 7 0.5473466931282829 1101 8 0.6116535232387719 1101 9 0.7324458404328595 1101 10-11 0.7794063118942844 1101 12-13 0.9138107514476701 1101 14-15 1.0387471850048229 1101 16-17 0.8062878762728545 1101 18-19 0.8803214699053967 1101 20-21 0.9691855277697101 1101 22-23 1.058405793714762 1101 24-25 0.9455038620101206 1101 26-27 1.0382668011837382 1101 28-29 1.0753826900956014 1101 30-31 1.00811940925567 1101 32-33 1.092554260878586 1101 34-35 0.9865657759610897 1101 36-37 1.01492492565297 1101 38-39 0.9676402321433883 1101 40-41 1.0663139137414603 1101 42-43 1.2438651156481058 1101 44-45 1.3709793004740405 1101 46-47 1.3877281840185915 1101 48-49 1.1975872730074926 1101 50-51 1.504022359651671 1101 52-53 1.2815521888605375 1101 54-55 1.3753442948577046 1101 56-57 1.1987833610371013 1101 58-59 1.3253148770144243 1101 60-61 1.4496641161955033 1101 62-63 1.350130776319066 1101 64-65 1.396825676209943 1101 66-67 1.3281102118614179 1101 68-69 1.3771234616407781 1101 70-71 1.4195990859153618 1101 72-73 1.4333230532846315 1101 74-75 1.3722963734406264 1101 76-77 1.4029881883467255 1101 78-79 1.7408831940006557 1101 80-81 1.605616594967966 1101 82-83 1.4364513962950696 1101 84-85 1.46873124463335 1101 86-87 1.5734654006531024 1101 88-89 1.5911653954136504 1101 90-91 1.3297422321409975 1101 92-93 1.4506160260504757 1101 94-95 1.511534301681241 1101 96-97 1.3488381856058922 1101 98-99 1.4075565042440719 1101 100-101 1.3679462877889055 1102 1 0.5642592995830569 1102 2 0.4906850339030804 1102 3 0.4672728751156683 1102 4 0.5287172893351482 1102 5 0.47812199945719414 1102 6 0.576243511599543 1102 7 0.517945867939801 1102 8 0.5061838511736241 1102 9 0.7252254412710073 1102 10-11 0.7443309412762531 1102 12-13 0.6596295281185149 1102 14-15 0.8007286683409944 1102 16-17 1.0111899612385926 1102 18-19 0.9308087376124874 1102 20-21 0.9486118823493896 1102 22-23 0.9837078260506829 1102 24-25 0.8988764909679077 1102 26-27 0.819309951746277 1102 28-29 0.7366403660962106 1102 30-31 0.9333336597618427 1102 32-33 1.004759666432605 1102 34-35 0.9255522002163659 1102 36-37 1.135589298602028 1102 38-39 1.1344440210978064 1102 40-41 1.1863817335510944 1102 42-43 1.3804981227943713 1102 44-45 1.3679796951976968 1102 46-47 1.2074822868863322 1102 48-49 1.430145740222848 1102 50-51 1.4572625303451048 1102 52-53 1.3145315964608315 1102 54-55 1.2168361833846078 1102 56-57 1.3705505545911265 1102 58-59 1.4056799276706364 1102 60-61 1.4445235278019588 1102 62-63 1.569164508500041 1102 64-65 1.5480569783856346 1102 66-67 1.2716972778863145 1102 68-69 1.3774829351069044 1102 70-71 1.6641882296399437 1102 72-73 1.6398445853623151 1102 74-75 1.545020231834986 1102 76-77 1.6907287292163353 1102 78-79 1.7611325179627322 1102 80-81 1.9215230258216103 1102 82-83 1.7645462458385843 1102 84-85 1.9567693011004401 1102 86-87 2.0902524482182905 1102 88-89 2.0622299906647896 1102 90-91 2.103767991296717 1102 92-93 1.9883419857101146 1102 94-95 2.2492360084192313 1102 96-97 2.302963489749313 1102 98-99 2.1084712419688554 1102 100-101 1.7528833087446092 1103 1 0.4585767169371948 1103 2 0.4682050702026146 1103 3 0.4047772181763314 1103 4 0.33552067273434716 1103 5 0.3873051735105335 1103 6 0.5267796659597153 1103 7 0.4192985740469055 1103 8 0.44872877391503607 1103 9 0.5805763549382874 1103 10-11 0.6085714193153038 1103 12-13 0.7129422175933371 1103 14-15 0.7883075617986322 1103 16-17 0.7909617125071406 1103 18-19 0.8199964134242705 1103 20-21 0.7192123572032045 1103 22-23 0.7561597594028697 1103 24-25 0.6906055568520983 1103 26-27 0.7614492091458089 1103 28-29 0.8509528215622311 1103 30-31 0.8699110293451326 1103 32-33 0.80372976476513 1103 34-35 0.9168038205301272 1103 36-37 1.1186598133800842 1103 38-39 1.019383761013735 1103 40-41 1.0506901041924905 1103 42-43 1.0759744471508554 1103 44-45 1.065345743938387 1103 46-47 0.8168471745977897 1103 48-49 0.837354065903007 1103 50-51 1.158785052471707 1103 52-53 1.056010135825204 1103 54-55 1.1105789551230956 1103 56-57 1.0788191880585956 1103 58-59 1.005546940805548 1103 60-61 1.1208699611208992 1103 62-63 1.0325122692461335 1103 64-65 1.2722160961085898 1103 66-67 1.2799343971665742 1103 68-69 1.436060353144665 1103 70-71 1.550528594881488 1103 72-73 1.578629920034345 1103 74-75 1.631507660153357 1103 76-77 1.5288316821970938 1103 78-79 1.588053709582141 1103 80-81 1.6780063937304028 1103 82-83 1.8599441321719077 1103 84-85 2.053996625440771 1103 86-87 2.28156356611553 1103 88-89 2.09878870152043 1103 90-91 1.87950177604543 1103 92-93 1.9777069804721457 1103 94-95 1.8017648810865161 1103 96-97 2.0846131334962656 1103 98-99 2.043984726021888 1103 100-101 1.7868052418301108 1104 1 0.49162026932387093 1104 2 0.3415125969389159 1104 3 0.44030815268728674 1104 4 0.3053462151867805 1104 5 0.43676480048954147 1104 6 0.4386995244535541 1104 7 0.4173715767609778 1104 8 0.558039425929806 1104 9 0.6186736218123059 1104 10-11 0.6368294902012863 1104 12-13 0.7025980727831325 1104 14-15 0.811870746133323 1104 16-17 0.8803485344174291 1104 18-19 0.8445699000457623 1104 20-21 0.8972507207248128 1104 22-23 0.8093211005102532 1104 24-25 0.8957814463843405 1104 26-27 0.8658159396590648 1104 28-29 0.9140533503040302 1104 30-31 0.9472549275032804 1104 32-33 1.0223234631872629 1104 34-35 0.8603518820275085 1104 36-37 1.0538101501909694 1104 38-39 1.098403463414492 1104 40-41 1.0822396267188878 1104 42-43 1.2058031242334977 1104 44-45 1.0205854545776774 1104 46-47 0.9453506476439699 1104 48-49 0.7394549605314964 1104 50-51 0.8607690773674719 1104 52-53 0.906190323109918 1104 54-55 1.080864531085325 1104 56-57 1.105964402275852 1104 58-59 1.2719563387973913 1104 60-61 1.2145594361638636 1104 62-63 1.1468572129104615 1104 64-65 1.168738904589791 1104 66-67 1.3013679932267586 1104 68-69 1.4208491239389005 1104 70-71 1.4036259844970473 1104 72-73 1.4394194727776437 1104 74-75 1.306724451844051 1104 76-77 1.4546009232380186 1104 78-79 1.6120559309792917 1104 80-81 1.4923626404072756 1104 82-83 1.689315176623321 1104 84-85 1.6813345483786826 1104 86-87 1.8228049947767495 1104 88-89 1.8033350573149853 1104 90-91 1.739853505653631 1104 92-93 1.9180024360046843 1104 94-95 2.1206135132466493 1104 96-97 1.9807670879006132 1104 98-99 2.172348867183116 1104 100-101 2.0305014744020475 1105 1 0.4364088282119596 1105 2 0.4421873075112117 1105 3 0.2855821076569214 1105 4 0.34802630898152387 1105 5 0.3455496288454185 1105 6 0.45471519931005133 1105 7 0.46023279564834496 1105 8 0.4334062826514966 1105 9 0.6420837133288302 1105 10-11 0.7260185699002406 1105 12-13 0.7118766608612077 1105 14-15 0.7282028889546837 1105 16-17 0.755952883684806 1105 18-19 0.7342921618355476 1105 20-21 0.6557506843516947 1105 22-23 0.7551601239506667 1105 24-25 0.7848284313136773 1105 26-27 0.7663070421829232 1105 28-29 0.8070160436751053 1105 30-31 0.9843545998837868 1105 32-33 1.0183292584932602 1105 34-35 0.9691298713647356 1105 36-37 0.9372375962618165 1105 38-39 0.9178369922914911 1105 40-41 1.0397644421198393 1105 42-43 1.1396804426934963 1105 44-45 1.1307315525690527 1105 46-47 0.9701414803968547 1105 48-49 0.9598051357105675 1105 50-51 0.8463284677103431 1105 52-53 0.9760561694428347 1105 54-55 0.8753622138777004 1105 56-57 0.8200318280300323 1105 58-59 0.920439274574651 1105 60-61 0.8728755303785327 1105 62-63 1.1011304016486463 1105 64-65 1.1803899787295435 1105 66-67 1.2889806573468725 1105 68-69 1.0656680881455678 1105 70-71 1.2106072285973077 1105 72-73 1.4115777407373642 1105 74-75 1.2411361285318385 1105 76-77 1.4155889828341834 1105 78-79 1.5117387224837522 1105 80-81 1.5356449163281347 1105 82-83 1.5949614181469656 1105 84-85 1.572226151675526 1105 86-87 1.885702229591626 1105 88-89 1.6789678952332956 1105 90-91 1.6879494923693805 1105 92-93 1.8583156194981676 1105 94-95 1.867464450083304 1105 96-97 2.15072550056874 1105 98-99 2.3089090411816873 1105 100-101 1.9581708805966311 1106 1 0.3287060567350082 1106 2 0.22672477819198278 1106 3 0.11629784439021407 1106 4 0.07842879235120392 1106 5 0.21076202749937067 1106 6 0.12274396025997447 1106 7 0.2940266543376069 1106 8 0.24066251789533055 1106 9 0.02488842088321519 1106 10-11 0.06714311514647164 1106 12-13 0.178603446426834 1106 14-15 0.42033550927186525 1106 16-17 0.2626247512155402 1106 18-19 0.14008610463120164 1106 20-21 0.38674182657643996 1106 22-23 0.42960830454938304 1106 24-25 0.1869291598637588 1106 26-27 0.14927833186848005 1106 28-29 0.34072107572563937 1106 30-31 0.20769811180586828 1106 32-33 0.3188896816341895 1106 34-35 0.014014529759798222 1106 36-37 0.03448100957346156 1106 38-39 0.2619793689490493 1106 40-41 0.5036475737265889 1106 42-43 -0.16100622584411184 1106 44-45 0.03258755729273588 1106 46-47 0.19677355187697998 1106 48-49 0.4855649896744225 1106 50-51 0.05093406456098393 1106 52-53 0.1089488883189631 1106 54-55 0.15374498545764226 1106 56-57 -0.6413765161813458 1106 58-59 -0.23436259910958057 1106 60-61 0.467145482686778 1106 62-63 0.4022488878024184 1106 64-65 0.37870613462158076 1106 66-67 -0.06238662921816385 1106 68-69 0.11698087720762018 1106 70-71 -0.18114704173262197 1106 72-73 0.29200519855329077 1106 74-75 0.7771387238144989 1106 76-77 0.4787816696983995 1106 78-79 0.70816342881945 1106 80-81 0.7634217357508746 1106 82-83 0.6639902726437832 1106 84-85 0.7759367879574732 1106 86-87 0.5682747863655777 1106 88-89 0.6992423395089524 1106 90-91 0.738728506282122 1106 92-93 0.9302466162188736 1106 94-95 0.9598277621926492 1106 96-97 1.0024101599936763 1106 98-99 1.0431060526587768 1106 100-101 0.47543762055600247 1107 1 0.20581258649516698 1107 2 0.027368600076389527 1107 3 0.16088492450584013 1107 4 0.06236324257425707 1107 5 0.15341454333615445 1107 6 0.22489888956351223 1107 7 0.18934271909624556 1107 8 0.19236829482131412 1107 9 0.20528727333687868 1107 10-11 0.406922908349479 1107 12-13 0.3584644895494762 1107 14-15 0.430757110252614 1107 16-17 0.4138555648717741 1107 18-19 0.3405077649473256 1107 20-21 0.4101995763215456 1107 22-23 0.4944039452446205 1107 24-25 0.5536301586430952 1107 26-27 0.46178684072741305 1107 28-29 0.6449982833892705 1107 30-31 0.6158978663551622 1107 32-33 0.7071585157565181 1107 34-35 0.5739528275528869 1107 36-37 0.5745031383754124 1107 38-39 0.6110685543170788 1107 40-41 0.5605341373276858 1107 42-43 0.7706329833734387 1107 44-45 0.5991354872624797 1107 46-47 0.5503134679437878 1107 48-49 0.6638823238806495 1107 50-51 0.6205000229279776 1107 52-53 0.6849660039402252 1107 54-55 0.7946622379788195 1107 56-57 0.800391655197707 1107 58-59 0.6728493861106308 1107 60-61 0.6580923534539771 1107 62-63 0.638758081655407 1107 64-65 0.5871936987491218 1107 66-67 0.7158673150191 1107 68-69 0.935032464709348 1107 70-71 1.1306460636935007 1107 72-73 1.1364874857873168 1107 74-75 0.9427522698190351 1107 76-77 1.1111299037949465 1107 78-79 1.1645175562382306 1107 80-81 1.218573003072276 1107 82-83 1.050230990364593 1107 84-85 1.1753628994163599 1107 86-87 1.3091225505588504 1107 88-89 1.2952125439160547 1107 90-91 1.205989301448792 1107 92-93 1.4172859552490635 1107 94-95 1.6032476493697132 1107 96-97 1.928006472365464 1107 98-99 1.873472699184374 1107 100-101 1.5871436513055759 1108 1 0.3567965591155762 1108 2 0.267006823046426 1108 3 0.3076487406928088 1108 4 0.24491632638913075 1108 5 0.22355522849994713 1108 6 0.3924291794696231 1108 7 0.4269172287038572 1108 8 0.44261082825340736 1108 9 0.3552620943569238 1108 10-11 0.5227574649346067 1108 12-13 0.3854707926791576 1108 14-15 0.49676472355263 1108 16-17 0.5981553625931681 1108 18-19 0.6125668509561706 1108 20-21 0.6816972200571669 1108 22-23 0.5516380994641636 1108 24-25 0.6906811812351066 1108 26-27 0.7563809477668855 1108 28-29 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0.5822047214601547 2202 4 0.4965684605823739 2202 5 0.5594669992677979 2202 6 0.5719483973427444 2202 7 0.6334201838329463 2202 8 0.5970148982886556 2202 9 0.5907733490430687 2202 10-11 0.7457179588030272 2202 12-13 0.7423359776891374 2202 14-15 0.8064002483478134 2202 16-17 0.8228067910519599 2202 18-19 0.7949190334410119 2202 20-21 0.7448259589606963 2202 22-23 0.7714603246573049 2202 24-25 0.7475848452695217 2202 26-27 0.6969317977119758 2202 28-29 0.7578621536213035 2202 30-31 0.6672374654359885 2202 32-33 0.7425442262070661 2202 34-35 0.6751382458974362 2202 36-37 0.877891912737077 2202 38-39 1.1510959423328515 2202 40-41 0.9580492660045721 2202 42-43 1.040767977720968 2202 44-45 1.152711815347267 2202 46-47 1.3212511380413403 2202 48-49 1.221736921101943 2202 50-51 1.0891456707703142 2202 52-53 1.1760093592677165 2202 54-55 1.0830730680672147 2202 56-57 1.2893839880310551 2202 58-59 1.4175030582475152 2202 60-61 1.38462744927509 2202 62-63 1.4483060808771135 2202 64-65 1.502671622087309 2202 66-67 1.651753743419885 2202 68-69 1.7601842440683377 2202 70-71 1.8622042250512756 2202 72-73 1.7158534975552975 2202 74-75 1.7470874331250066 2202 76-77 1.8770302232397 2202 78-79 2.3025934249826427 2202 80-81 2.4495513857508335 2202 82-83 2.349229800759229 2202 84-85 2.5833304229436997 2202 86-87 2.6090072844493015 2202 88-89 2.9371984706137084 2202 90-91 3.0617652911241002 2202 92-93 3.1573127275270743 2202 94-95 3.3497920058868047 2202 96-97 3.3520688884711944 2202 98-99 3.576272089357696 2202 100-101 3.205000292776253 2203 1 0.49133317962039413 2203 2 0.48237323744861627 2203 3 0.5280199125583636 2203 4 0.4264945395568134 2203 5 0.3859196984732378 2203 6 0.39605860917644264 2203 7 0.5385680364472236 2203 8 0.4817838713166722 2203 9 0.5726106213804698 2203 10-11 0.6521114197816402 2203 12-13 0.6321970298225779 2203 14-15 0.8157144243259182 2203 16-17 0.7692158432614562 2203 18-19 0.8544676060405862 2203 20-21 0.8752856890084644 2203 22-23 0.8426264768978555 2203 24-25 0.8861610996981213 2203 26-27 0.8322271332297717 2203 28-29 1.0130272241938059 2203 30-31 0.9231364646817823 2203 32-33 0.8913542857199346 2203 34-35 1.0311090746622043 2203 36-37 1.0332792322918252 2203 38-39 1.1798571155201572 2203 40-41 1.2358683105452073 2203 42-43 1.198358984665191 2203 44-45 1.1629530146467175 2203 46-47 1.3180608510824143 2203 48-49 1.190472066651786 2203 50-51 1.3735958474920622 2203 52-53 1.2482726017789574 2203 54-55 1.2626333446531106 2203 56-57 1.3317781926564507 2203 58-59 1.4733329743971133 2203 60-61 1.4817745497920463 2203 62-63 1.4922957458769126 2203 64-65 1.5781790236967126 2203 66-67 1.7996243150772244 2203 68-69 1.9034670220961125 2203 70-71 1.8750429065148246 2203 72-73 1.9555786017609158 2203 74-75 1.9402915155768419 2203 76-77 1.8985790789320838 2203 78-79 2.053233024596455 2203 80-81 2.187998558308575 2203 82-83 2.362658329331534 2203 84-85 2.5281112689686758 2203 86-87 2.5352630862674346 2203 88-89 2.5581733239441675 2203 90-91 2.7941158276272304 2203 92-93 3.1471129358326806 2203 94-95 3.296315985326661 2203 96-97 3.5065027767931944 2203 98-99 3.501513616115245 2203 100-101 3.3156876801465103 2204 1 0.5245436437975748 2204 2 0.5310232204536973 2204 3 0.5072215661997816 2204 4 0.4495765338678126 2204 5 0.5822688290499727 2204 6 0.4699464058174456 2204 7 0.5104794348630719 2204 8 0.40656774663640505 2204 9 0.6146261728855507 2204 10-11 0.6567405763599723 2204 12-13 0.6899824428578327 2204 14-15 0.8751974015342796 2204 16-17 0.9985970646912321 2204 18-19 0.8758620110025177 2204 20-21 0.8687111902748015 2204 22-23 0.9749164911350334 2204 24-25 0.851225200515934 2204 26-27 1.0627876470924704 2204 28-29 1.0802769011645807 2204 30-31 1.0141626016545615 2204 32-33 0.9346383163200116 2204 34-35 1.1188560091850306 2204 36-37 1.126337215776367 2204 38-39 1.121188427624439 2204 40-41 1.11913575276143 2204 42-43 1.3842051284526988 2204 44-45 1.4084110958677698 2204 46-47 1.449384690450863 2204 48-49 1.4327156141350983 2204 50-51 1.3631584933686227 2204 52-53 1.3727033347985937 2204 54-55 1.3871557789963518 2204 56-57 1.4394372649056137 2204 58-59 1.4785586086890525 2204 60-61 1.7724071003252213 2204 62-63 1.834943189912071 2204 64-65 2.0288467291979906 2204 66-67 2.1587302187899944 2204 68-69 2.0507432035993496 2204 70-71 2.2245899950187535 2204 72-73 2.2278917173552983 2204 74-75 2.22111185684156 2204 76-77 2.3245209139728047 2204 78-79 2.455515624773234 2204 80-81 2.6285468886361905 2204 82-83 2.6954705130342624 2204 84-85 2.880199045743847 2204 86-87 3.059357692863305 2204 88-89 3.1540403519956364 2204 90-91 3.366227686477604 2204 92-93 3.6611570270696276 2204 94-95 3.744210228721588 2204 96-97 3.8603187547010513 2204 98-99 3.6827346267828585 2204 100-101 3.276409631087981 2205 1 0.4676167818775028 2205 2 0.3683816619556346 2205 3 0.4399773877434576 2205 4 0.42229013133795235 2205 5 0.3655788623154592 2205 6 0.4446788812411171 2205 7 0.42097271824973603 2205 8 0.408551753363561 2205 9 0.5061691034335283 2205 10-11 0.5283975258549773 2205 12-13 0.4924241593391514 2205 14-15 0.6469337577933345 2205 16-17 0.722484909196119 2205 18-19 0.6131400743276672 2205 20-21 0.5392955423599446 2205 22-23 0.5806379543914701 2205 24-25 0.6477427937968798 2205 26-27 0.7578425086644955 2205 28-29 0.7295665161646525 2205 30-31 0.7833271219418876 2205 32-33 0.8064308899775909 2205 34-35 0.8698834649620864 2205 36-37 0.806609583301821 2205 38-39 0.899170594147165 2205 40-41 1.0131735950162906 2205 42-43 0.9371665922604322 2205 44-45 1.0555030763657882 2205 46-47 1.1806504146755898 2205 48-49 1.0305517798146013 2205 50-51 1.1496841103527018 2205 52-53 1.1083904814472163 2205 54-55 0.9903232570278888 2205 56-57 1.1361943819983793 2205 58-59 1.314623950393031 2205 60-61 1.2744027723078695 2205 62-63 1.3122338849238453 2205 64-65 1.473510866981183 2205 66-67 1.5801697046752032 2205 68-69 1.646000642088584 2205 70-71 1.768051208334846 2205 72-73 1.8340156111694235 2205 74-75 1.7656120666501032 2205 76-77 1.8970169897144373 2205 78-79 2.013777930123293 2205 80-81 2.1695879558152775 2205 82-83 2.355461401680973 2205 84-85 2.2724365363971124 2205 86-87 2.3934352086105726 2205 88-89 2.6768652301912716 2205 90-91 2.867479688044483 2205 92-93 3.050385006346822 2205 94-95 3.2161758383860324 2205 96-97 3.215095456802974 2205 98-99 3.1725531004626415 2205 100-101 2.8358276685121115 2206 1 0.31649725269427265 2206 2 0.24742170260168805 2206 3 0.3596565646204155 2206 4 0.2649029019510749 2206 5 0.21639371479049885 2206 6 -0.2967389500889013 2206 7 0.21047817272526004 2206 8 0.29931009001293774 2206 9 0.04782337886822319 2206 10-11 0.1673471081179443 2206 12-13 0.34597767790643275 2206 14-15 0.4660578974426244 2206 16-17 0.06910016722078893 2206 18-19 0.19131781174127838 2206 20-21 0.5177130806861712 2206 22-23 0.5170938753705201 2206 24-25 0.2965354140823564 2206 26-27 0.44843067708043804 2206 28-29 0.4579461849742188 2206 30-31 0.5335949496705013 2206 32-33 0.6197833863546833 2206 34-35 0.4896789577642622 2206 36-37 0.30520073607183207 2206 38-39 0.700343865994121 2206 40-41 0.8826825361862873 2206 42-43 0.9323952440162415 2206 44-45 0.8921866108517591 2206 46-47 1.0676798268917764 2206 48-49 1.1213084380600051 2206 50-51 1.223196643813246 2206 52-53 1.1151522565474963 2206 54-55 1.2047153686304526 2206 56-57 0.7991742811288347 2206 58-59 1.0271045369943756 2206 60-61 1.140201934657778 2206 62-63 0.726770313965055 2206 64-65 0.9572272700147266 2206 66-67 1.4174983282439406 2206 68-69 1.103075460614317 2206 70-71 1.080245858999941 2206 72-73 1.5421023217354914 2206 74-75 1.8087392469938521 2206 76-77 2.0860026197088466 2206 78-79 2.2821244036447403 2206 80-81 2.3504398208931896 2206 82-83 2.268625714393103 2206 84-85 2.4480115500056776 2206 86-87 2.2451841055449293 2206 88-89 1.6463296641364629 2206 90-91 1.7391299859148006 2206 92-93 1.988336351524346 2206 94-95 2.244214040547174 2206 96-97 2.469093136725771 2206 98-99 2.4869531233361624 2206 100-101 1.9126485976529324 2207 1 0.3461416473250587 2207 2 0.31266839769800825 2207 3 0.3018952583032508 2207 4 0.3005408218210306 2207 5 0.37148782515934187 2207 6 0.3065039766570976 2207 7 0.39195306017334275 2207 8 0.24113754148108058 2207 9 0.43413823763147263 2207 10-11 0.3278152234786518 2207 12-13 0.45113840652501835 2207 14-15 0.5061896327793249 2207 16-17 0.5390298342573985 2207 18-19 0.4727620449140417 2207 20-21 0.5668047152988365 2207 22-23 0.6161363693199959 2207 24-25 0.5400198652990085 2207 26-27 0.6254927437681062 2207 28-29 0.6305844710847168 2207 30-31 0.6762683495393418 2207 32-33 0.8031942174932141 2207 34-35 0.7049517613028229 2207 36-37 0.6564176119976608 2207 38-39 0.9006023017941303 2207 40-41 1.0378536143590864 2207 42-43 0.9089055337481255 2207 44-45 1.067643354987446 2207 46-47 0.9900291516217621 2207 48-49 0.9217559556075443 2207 50-51 0.9955771500521635 2207 52-53 1.056077100683538 2207 54-55 1.1034904181426697 2207 56-57 1.1897436660265512 2207 58-59 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2316 84-85 -3.0224463736583154 2316 86-87 -3.088453674658293 2316 88-89 -3.4074598602024615 2316 90-91 -3.0782599675221967 2316 92-93 -3.3316934218096996 2316 94-95 -3.6272231231970586 2316 96-97 -3.7695670315116008 2316 98-99 -3.7397071120365037 2316 100-101 -3.1874333007261093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13500.0 3 4982.0 4 5366.0 5 6117.0 6 7181.0 7 9077.0 8 11145.0 9 14052.0 10 17093.0 11 20710.0 12 24875.0 13 28668.0 14 32023.0 15 36114.0 16 40479.0 17 45732.0 18 45618.0 19 44726.0 20 48601.0 21 52308.0 22 57359.0 23 63749.0 24 70277.0 25 78174.0 26 87603.0 27 97833.0 28 110113.0 29 125315.0 30 142463.0 31 163276.0 32 190189.0 33 223689.0 34 266100.0 35 316012.0 36 357202.0 37 320438.0 38 125803.0 39 4529.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57129103760054 18.117678779618515 14.421887889073377 23.88914229370757 2 15.743419591544788 19.363892336487506 39.97721054947023 24.915477522497472 3 19.670276366407066 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26.558500044361555 27.014872381553513 23.16672281766464 48-49 23.134778508844516 26.42072475208797 27.090337752228983 23.35415898683853 50-51 23.304659698643448 26.65142679230369 26.99931911767929 23.044594391373575 52-53 23.52704774151502 26.35699687651991 26.995612981078054 23.12034240088702 54-55 22.975726446533688 26.50945488000462 27.247414797558662 23.267403875903028 56-57 23.053705497333134 26.6917452336817 27.098485975228563 23.1560632937566 58-59 23.059097104435818 26.220877670609678 27.152665239852464 23.56735998510204 60-61 22.774675050027767 26.460207705561302 27.41792316810993 23.347194076301 62-63 22.87593909390531 26.526423403823756 27.41913884941518 23.178498652855755 64-65 22.641403065427117 26.480985797348094 27.26755003121047 23.610061106014324 66-67 22.587248634576852 26.55090546568275 27.247401252428865 23.614444647311533 68-69 23.071266793073665 26.154791386892317 27.404422028069003 23.36951979196502 70-71 22.905686761376735 26.30418334297063 27.07921858524473 23.710911310407905 72-73 22.95286046254288 26.39079110711217 27.025673445718905 23.630674984626047 74-75 22.8702343159486 26.74393046107332 26.942010582010585 23.4438246409675 76-77 23.070223120862323 26.411941903078684 26.962790616117356 23.55504435994164 78-79 23.175439354813943 26.534806066045064 26.982722635104196 23.3070319440368 80-81 23.213370274495485 26.672342249429015 26.693274298406084 23.421013177669415 82-83 23.183914014949085 26.722956470642288 26.68999388204683 23.403135632361796 84-85 23.164564518725125 26.617039300128692 26.74653090803572 23.471865273110463 86-87 23.291996720720764 26.59393284974444 26.56373373510147 23.550336694433327 88-89 23.184136720835273 26.641404883323244 26.470023075163528 23.70443532067795 90-91 23.157713956745436 26.724274514831414 26.494721985036758 23.623289543386395 92-93 23.326669181155843 26.252854310547473 26.703633184907925 23.716843323388755 94-95 23.12375828941042 26.251598915005758 26.767802765707167 23.856840029876654 96-97 23.408085932403193 26.026329392728808 26.6670940346038 23.898490640264196 98-99 23.20511992976226 26.17779804745008 26.33399780839627 24.28308421439139 100-101 23.863071787628513 25.50195017786705 26.700066742680423 23.934911291824015 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 394.0 1 289.5 2 260.5 3 373.0 4 338.0 5 307.5 6 556.5 7 1204.5 8 1903.5 9 2184.0 10 1949.5 11 1642.5 12 1639.5 13 1775.5 14 2031.0 15 2270.0 16 2432.5 17 2634.5 18 2903.0 19 3301.5 20 3713.5 21 4324.5 22 5275.5 23 6360.5 24 7672.5 25 9646.5 26 12375.5 27 15748.0 28 19257.0 29 23324.0 30 28582.5 31 35578.0 32 44693.5 33 49867.0 34 52192.0 35 57265.5 36 64523.5 37 71973.5 38 77787.5 39 82597.0 40 88922.0 41 97955.0 42 105740.0 43 111171.5 44 116375.5 45 123511.5 46 127340.0 47 129340.5 48 131733.5 49 130742.5 50 129066.5 51 126197.5 52 123410.5 53 122872.5 54 123500.5 55 117173.5 56 106070.0 57 97271.0 58 90539.5 59 80954.5 60 67008.0 61 56181.0 62 47375.0 63 37019.0 64 27963.5 65 21789.5 66 16413.0 67 12459.5 68 9489.0 69 7114.5 70 5682.0 71 4266.0 72 3253.5 73 2661.0 74 2043.0 75 1572.0 76 1175.0 77 764.0 78 512.5 79 339.0 80 195.0 81 112.0 82 89.5 83 61.5 84 26.0 85 22.0 86 15.5 87 12.0 88 11.5 89 7.5 90 4.5 91 2.5 92 3.0 93 2.0 94 1.0 95 1.5 96 1.5 97 2.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006498428891470797 2 0.1043980157727448 3 0.11440257374054402 4 0.11700194529713234 5 0.1564761226564852 6 0.09789958688127401 7 0.025993715565883188 8 0.02820015886391746 9 0.02272938849481879 10-11 0.052183895261043396 12-13 0.054360113308419666 14-15 0.027595653850757382 16-17 0.03193297732018091 18-19 0.03713172043335755 20-21 0.059921559429492344 22-23 0.03161561218827188 24-25 0.04888934293932099 26-27 0.058017368638038115 28-29 0.054254324931116656 30-31 0.0692762745081445 32-33 0.05091443473340724 34-35 0.0635637021337818 36-37 0.054118311303155635 38-39 0.032582820209328 40-41 0.03717705830934456 42-43 0.033414014602423096 44-45 0.0074051864112109084 46-47 0.01352579966945666 48-49 0.009445390830626158 50-51 0.008599083812202056 52-53 0.009762755962535197 54-55 0.01165183412866043 56-57 0.014749922321105807 58-59 0.019842877056979433 60-61 0.025056732795485072 62-63 0.01157627100201542 64-65 0.00927915195200714 66-67 0.010881090236881335 68-69 0.011923861384582462 70-71 0.031116895552414814 72-73 0.02871398812510352 74-75 0.029983448652739678 76-77 0.023515245011926884 78-79 0.005289418865150649 80-81 0.005077842110544623 82-83 0.005924149128968726 84-85 0.005123179986531628 86-87 0.013540912294785662 88-89 0.03797802745178166 90-91 0.0359075977817084 92-93 0.03005901177938469 94-95 0.04721184152780179 96-97 0.04314654531430029 98-99 0.026250630196476218 100-101 0.0759107170275763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3308492.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.68770882393612 #Duplication Level Percentage of deduplicated Percentage of total 1 77.26891396390444 41.483909540358724 2 12.322841256932806 13.231702265715908 3 4.30525126273344 6.934172286225484 4 1.9951623440277368 4.28462779930572 5 1.049228288641071 2.816533142519931 6 0.6623070503900264 2.133464884402783 7 0.4327942387339437 1.6265011748877547 8 0.3129428610317466 1.3440948161281534 9 0.2404441649838736 1.1618006686261797 >10 1.261848622669464 12.373115927327001 >50 0.09228711574126154 3.3934795034794734 >100 0.050085293638701706 5.00249724594514 >500 0.0032301113921185324 1.2064974822781178 >1k 0.0025500879377632676 2.6015938598089123 >5k 1.1333724167836745E-4 0.4060094029908425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7396 0.22354595386659543 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5949 0.17981001616446404 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4205 0.1270971790169056 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3679 0.11119869717079564 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3532 0.10675558532406909 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0225250658003708E-5 2 0.0 0.0 0.0 0.0 3.0225250658003708E-5 3 0.0 0.0 0.0 3.0225250658003708E-5 3.0225250658003708E-5 4 0.0 0.0 0.0 3.0225250658003708E-5 3.0225250658003708E-5 5 3.0225250658003708E-5 0.0 0.0 6.0450501316007416E-5 3.0225250658003708E-5 6 3.0225250658003708E-5 0.0 0.0 6.0450501316007416E-5 3.0225250658003708E-5 7 6.0450501316007416E-5 0.0 0.0 9.067575197401112E-5 3.0225250658003708E-5 8 6.0450501316007416E-5 0.0 0.0 9.067575197401112E-5 3.0225250658003708E-5 9 6.0450501316007416E-5 0.0 0.0 9.067575197401112E-5 3.0225250658003708E-5 10-11 6.0450501316007416E-5 0.0 0.0 9.067575197401112E-5 3.0225250658003708E-5 12-13 6.0450501316007416E-5 0.0 0.0 9.067575197401112E-5 3.0225250658003708E-5 14-15 6.0450501316007416E-5 0.0 0.0 9.067575197401112E-5 3.0225250658003708E-5 16-17 6.0450501316007416E-5 0.0 0.0 1.3601362796101667E-4 3.0225250658003708E-5 18-19 6.0450501316007416E-5 0.0 0.0 2.1157675460602594E-4 3.0225250658003708E-5 20-21 6.0450501316007416E-5 0.0 0.0 3.324777572380408E-4 3.0225250658003708E-5 22-23 6.0450501316007416E-5 0.0 0.0 5.13829261186063E-4 3.0225250658003708E-5 24-25 9.067575197401112E-5 0.0 0.0 8.916448944111093E-4 3.0225250658003708E-5 26-27 9.067575197401112E-5 0.0 0.0 0.0014054741555971724 3.0225250658003708E-5 28-29 1.2090100263201483E-4 0.0 0.0 0.002992299815142367 3.0225250658003708E-5 30-31 1.5112625329001853E-4 0.0 0.0 0.009430278205297156 3.0225250658003708E-5 32-33 1.662388786190204E-4 0.0 0.0 0.02322810513067585 3.0225250658003708E-5 34-35 1.9646412927702407E-4 0.0 0.0 0.04415909121134341 3.0225250658003708E-5 36-37 2.1157675460602594E-4 0.0 0.0 0.07952263448120775 3.0225250658003708E-5 38-39 2.1157675460602594E-4 0.0 0.0 0.13418500029620745 3.0225250658003708E-5 40-41 2.1157675460602594E-4 0.0 0.0 0.19128049878917647 3.0225250658003708E-5 42-43 2.266893799350278E-4 0.0 0.0 0.25236573036900195 3.0225250658003708E-5 44-45 2.4180200526402966E-4 0.0 0.0 0.33208482897948677 3.0225250658003708E-5 46-47 2.569146305930315E-4 0.0 0.0 0.42755128318279145 3.0225250658003708E-5 48-49 2.7202725592203334E-4 0.0 0.0 0.529334814773619 3.0225250658003708E-5 50-51 2.7202725592203334E-4 0.0 0.0 0.6410775664562587 3.0225250658003708E-5 52-53 2.7202725592203334E-4 0.0 0.0 0.7526691918856083 3.0225250658003708E-5 54-55 2.7202725592203334E-4 0.0 0.0 0.872043819359394 3.0225250658003708E-5 56-57 2.7202725592203334E-4 0.0 0.0 1.0139060333227343 3.0225250658003708E-5 58-59 2.7202725592203334E-4 0.0 0.0 1.1705937327338256 3.0225250658003708E-5 60-61 2.7202725592203334E-4 0.0 0.0 1.3413210610755595 3.0225250658003708E-5 62-63 2.7202725592203334E-4 0.0 0.0 1.5109451677682761 3.0225250658003708E-5 64-65 2.7202725592203334E-4 0.0 0.0 1.6931883166107098 3.0225250658003708E-5 66-67 2.7202725592203334E-4 0.0 0.0 1.9043872555835106 3.0225250658003708E-5 68-69 3.0225250658003707E-4 0.0 0.0 2.1420484015073935 6.0450501316007416E-5 70-71 3.1736513190903893E-4 0.0 0.0 2.3937945142379067 6.0450501316007416E-5 72-73 3.324777572380408E-4 0.0 0.0 2.649681486308566 7.556312664500927E-5 74-75 3.324777572380408E-4 0.0 0.0 2.90777490167726 1.2090100263201483E-4 76-77 3.324777572380408E-4 0.0 0.0 3.193947574907239 1.2090100263201483E-4 78-79 3.324777572380408E-4 0.0 0.0 3.5163603236761642 1.5112625329001853E-4 80-81 3.324777572380408E-4 0.0 0.0 3.858223021243515 1.8135150394802223E-4 82-83 3.324777572380408E-4 0.0 0.0 4.203017568124692 1.8135150394802223E-4 84-85 3.324777572380408E-4 0.0 0.0 4.563453682221386 1.8135150394802223E-4 86-87 3.324777572380408E-4 0.0 0.0 4.9333956376500225 1.8135150394802223E-4 88-89 3.324777572380408E-4 0.0 0.0 5.330933246929417 1.8135150394802223E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4580 0.0 51.503777 1 GTATCAA 8755 0.0 46.11842 1 ATCAACG 11265 0.0 35.292694 3 TCAACGC 11400 0.0 34.958183 4 TATCAAC 11405 0.0 34.901165 2 CAACGCA 11710 0.0 34.073345 5 AACGCAG 12165 0.0 32.96993 6 ACGCAGA 13995 0.0 28.352287 7 CGCAGAG 14205 0.0 27.866236 8 GTACATG 11665 0.0 25.725718 1 GCAGAGT 15620 0.0 24.39766 9 TACATGG 11845 0.0 24.33029 2 GTAAGGT 1750 0.0 24.185867 4 TAAGGTG 1970 0.0 23.898943 5 ACATGGG 12030 0.0 23.046907 3 GGACCGA 1180 0.0 22.149727 6 GAGTACT 8605 0.0 22.14193 12-13 TGGTATC 2475 0.0 21.328362 2 GTGGTAT 2775 0.0 21.25111 1 AGAGTAC 14210 0.0 21.083813 10-11 >>END_MODULE