FastQCFastQC Report
Thu 26 May 2016
SRR938431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308492
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78140.236180108641641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65040.19658503027965613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47260.14284453460972552No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36140.10923405587802539No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35040.10590927830564499No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34290.10364238450629472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51900.045.9655761
GTATCAA87500.041.3307231
ATCAACG114250.031.4324383
TATCAAC116150.031.0950152
TCAACGC116400.030.8518564
CAACGCA118250.030.4093555
AACGCAG122900.029.2846096
GTACATG116450.025.7913441
ACGCAGA141250.025.3793547
CGCAGAG143400.024.932628
TACATGG119800.024.6337852
GTAAGGT18350.023.556884
GTGGTAT28050.023.5493161
ACATGGG121700.023.2240983
GGACCGA12200.022.9622426
TAAGGTG19300.022.8895975
TGGTATC28100.021.6471162
GCAGAGT163700.021.5797469
GTATAGG8350.021.0577341
AGGACCG14750.020.9331135
Produced by FastQC (version 0.11.5)