##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938431_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3308492 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80470196089336 31.0 31.0 34.0 30.0 34.0 2 32.034273922983644 31.0 31.0 34.0 30.0 34.0 3 32.096664583139386 33.0 31.0 34.0 30.0 34.0 4 35.76518063214298 37.0 35.0 37.0 35.0 37.0 5 35.661449385399756 37.0 35.0 37.0 33.0 37.0 6 35.6597105267294 37.0 35.0 37.0 33.0 37.0 7 35.5991642113688 37.0 35.0 37.0 33.0 37.0 8 35.57441758964507 37.0 35.0 37.0 33.0 37.0 9 37.16493163652806 39.0 37.0 39.0 33.0 39.0 10-11 37.06356823592138 39.0 37.0 39.0 33.0 39.0 12-13 36.953138771379834 39.0 37.0 39.0 33.0 39.0 14-15 38.00903145602287 40.0 37.0 41.0 33.0 41.0 16-17 37.91402034522072 40.0 37.0 41.0 33.0 41.0 18-19 38.14980525871001 40.0 37.0 41.0 33.0 41.0 20-21 38.11494587262112 40.0 37.0 41.0 33.0 41.0 22-23 38.00882909796971 40.0 37.0 41.0 33.0 41.0 24-25 37.883180917469346 40.0 37.0 41.0 32.0 41.0 26-27 37.68738249933807 40.0 37.0 41.0 32.0 41.0 28-29 37.57784256392338 39.5 37.0 41.0 32.0 41.0 30-31 37.33523354446678 39.0 36.0 41.0 31.0 41.0 32-33 37.151191388705186 39.0 36.0 41.0 31.0 41.0 34-35 37.05995178468015 39.0 36.0 41.0 31.0 41.0 36-37 37.02003359838863 39.0 36.0 41.0 31.0 41.0 38-39 36.860365387010155 39.0 35.5 41.0 30.0 41.0 40-41 36.692862035029854 39.0 35.0 41.0 30.0 41.0 42-43 36.48980124479672 39.0 35.0 40.0 30.0 41.0 44-45 36.20598387422427 39.0 35.0 40.0 30.0 41.0 46-47 35.845374569441304 38.0 35.0 40.0 28.5 41.0 48-49 35.50080973446512 38.0 34.5 40.0 27.5 41.0 50-51 35.12418905652485 38.0 34.0 40.0 26.5 41.0 52-53 34.62886928546298 38.0 33.0 40.0 25.5 41.0 54-55 34.282698734045596 37.5 33.0 40.0 24.0 41.0 56-57 33.93933444602556 37.0 33.0 40.0 24.0 41.0 58-59 33.45000108810902 36.5 32.0 39.0 23.0 41.0 60-61 33.04027408861802 36.0 31.5 39.0 22.0 40.0 62-63 32.54711859662952 35.5 31.0 39.0 20.5 40.0 64-65 32.004165644045685 35.0 30.5 38.0 19.0 40.0 66-67 31.482052095033026 35.0 30.0 38.0 17.5 40.0 68-69 30.965469162385766 34.0 29.0 37.5 14.0 39.5 70-71 30.369388379962835 34.0 29.0 37.0 9.5 39.0 72-73 29.841958813864444 34.0 28.5 36.0 7.5 39.0 74-75 29.23271689942125 33.5 27.5 36.0 7.0 38.5 76-77 28.023311375696238 31.5 26.0 34.5 4.0 36.5 78-79 28.703707459470962 33.0 27.5 35.0 2.0 37.0 80-81 28.30346257449013 33.0 26.5 35.0 2.0 36.5 82-83 27.834797394099787 33.0 26.0 35.0 2.0 36.0 84-85 27.268997627922328 32.0 26.0 35.0 2.0 36.0 86-87 26.637056701361224 32.0 25.0 34.5 2.0 35.0 88-89 25.862404080167035 31.5 23.5 34.0 2.0 35.0 90-91 25.220006274762035 31.0 20.5 34.0 2.0 35.0 92-93 24.353315800672934 31.0 17.5 34.0 2.0 35.0 94-95 23.515202394323456 30.5 6.0 34.0 2.0 35.0 96-97 22.406571634448564 30.0 2.0 34.0 2.0 35.0 98-99 20.995895713213148 29.0 2.0 34.0 2.0 35.0 100-101 17.019257413951735 22.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25235511553679046 1101 2 0.33250895119826396 1101 3 0.2647674492409422 1101 4 0.21711766795910847 1101 5 0.09448581605950324 1101 6 0.21974037236201838 1101 7 0.19104347651478548 1101 8 0.23256242569776475 1101 9 0.36930460107198115 1101 10-11 0.30273461158083137 1101 12-13 0.36860502194705447 1101 14-15 0.37962259173868773 1101 16-17 0.3360676526984747 1101 18-19 0.34938558315534607 1101 20-21 0.3679012235660153 1101 22-23 0.3087893326230926 1101 24-25 0.37896589880882203 1101 26-27 0.3719150844118886 1101 28-29 0.3195548712660141 1101 30-31 0.49103050724156105 1101 32-33 0.3850354183065363 1101 34-35 0.44981598416772073 1101 36-37 0.4986944702078162 1101 38-39 0.5517299779115135 1101 40-41 0.6707369780011518 1101 42-43 0.637441682186342 1101 44-45 0.6287755076273385 1101 46-47 0.45531939465886495 1101 48-49 0.531650187542084 1101 50-51 0.651410229951658 1101 52-53 0.7580486633422723 1101 54-55 0.8165520760550962 1101 56-57 0.8186692685655146 1101 58-59 0.8777314556003049 1101 60-61 0.763009266837166 1101 62-63 0.8604593239993577 1101 64-65 0.8076999350074914 1101 66-67 0.6653800992116885 1101 68-69 0.8592994310940476 1101 70-71 0.794051862942549 1101 72-73 0.8532528827306081 1101 74-75 0.7773539958433879 1101 76-77 0.7054805463882694 1101 78-79 0.9686649601196144 1101 80-81 0.9397754665725238 1101 82-83 0.8449285906795438 1101 84-85 1.0078560922415747 1101 86-87 1.1582569486059278 1101 88-89 1.0784299374839819 1101 90-91 1.0951566462044084 1101 92-93 1.4697359728312236 1101 94-95 1.2650935477348462 1101 96-97 1.2588691710526057 1101 98-99 1.3524556092887963 1101 100-101 1.4615055575915932 1102 1 0.3132178714934817 1102 2 0.3218536706400741 1102 3 0.2190900661213675 1102 4 0.2623095016960022 1102 5 0.17184036235313016 1102 6 0.14881314487353592 1102 7 0.19168966468210868 1102 8 0.16370985400435956 1102 9 0.2201923365560674 1102 10-11 0.41183441579003954 1102 12-13 0.3961351319001736 1102 14-15 0.432203318619365 1102 16-17 0.4719652169981714 1102 18-19 0.43144470529777124 1102 20-21 0.4532466165909881 1102 22-23 0.4073320953352635 1102 24-25 0.4533997354639254 1102 26-27 0.5262143811753504 1102 28-29 0.4120590001540947 1102 30-31 0.5107900105574359 1102 32-33 0.5724340126189844 1102 34-35 0.5893226679587187 1102 36-37 0.6448588264645778 1102 38-39 0.5313477047352464 1102 40-41 0.5732538814038293 1102 42-43 0.5487421690121934 1102 44-45 0.6704428125287833 1102 46-47 0.6785981736469964 1102 48-49 0.6473699592700513 1102 50-51 0.814957008633435 1102 52-53 0.6932379343210471 1102 54-55 0.6029899797385951 1102 56-57 0.618532980892482 1102 58-59 0.7985916684368775 1102 60-61 0.8005191594762593 1102 62-63 1.0077503610228646 1102 64-65 1.0586948939346286 1102 66-67 0.9401490582213015 1102 68-69 0.8588696536121496 1102 70-71 0.8487091112276595 1102 72-73 0.9049897650420817 1102 74-75 1.1219058273808251 1102 76-77 1.0529208812553321 1102 78-79 1.2360758085398338 1102 80-81 1.2619974269548386 1102 82-83 1.113780867803051 1102 84-85 1.1083488585867656 1102 86-87 1.073988601319801 1102 88-89 1.074345431271503 1102 90-91 1.1622175946802038 1102 92-93 1.5040655415612534 1102 94-95 1.4807570566122017 1102 96-97 1.5763719252811548 1102 98-99 1.867923425563589 1102 100-101 1.9797986919527801 1103 1 0.24176226912365095 1103 2 0.24307580884950397 1103 3 0.27381000115481413 1103 4 0.199456070462098 1103 5 0.15569103168216003 1103 6 0.13360698612241606 1103 7 0.17520791380044187 1103 8 0.17348732778354048 1103 9 0.3339632475714964 1103 10-11 0.32140602379862315 1103 12-13 0.3884278660207272 1103 14-15 0.5121667185907413 1103 16-17 0.4430304791612798 1103 18-19 0.46268976359900904 1103 20-21 0.3954740029079673 1103 22-23 0.3561774140135583 1103 24-25 0.530245569111834 1103 26-27 0.4514130257272413 1103 28-29 0.5085551878389154 1103 30-31 0.4244094450073632 1103 32-33 0.5388485845688038 1103 34-35 0.618572688523706 1103 36-37 0.6548703559994564 1103 38-39 0.5742426828612466 1103 40-41 0.5958335711675034 1103 42-43 0.6858275687033668 1103 44-45 0.6588460423761333 1103 46-47 0.624646842970769 1103 48-49 0.6129461092279982 1103 50-51 0.674591917338077 1103 52-53 0.6723850924287404 1103 54-55 0.6513380578802526 1103 56-57 0.5878282462896465 1103 58-59 0.6656281385023419 1103 60-61 0.9313305033065475 1103 62-63 0.98996343824615 1103 64-65 0.9044145423730257 1103 66-67 0.7253148438805113 1103 68-69 0.8894278040486334 1103 70-71 0.7631933546974814 1103 72-73 0.8779343282438035 1103 74-75 0.8576656592250309 1103 76-77 0.7423177188686232 1103 78-79 0.9616147518492291 1103 80-81 0.9393896223292764 1103 82-83 1.0315174768088937 1103 84-85 1.2131663505006927 1103 86-87 1.2002554603770115 1103 88-89 1.126933181755767 1103 90-91 1.1071448153129424 1103 92-93 1.551811413259074 1103 94-95 1.3231053161764201 1103 96-97 1.6699420169277168 1103 98-99 1.9064256208516355 1103 100-101 1.9084767619473304 1104 1 0.2376951628594135 1104 2 0.22035828228315069 1104 3 0.23324774237229917 1104 4 0.15855984677430968 1104 5 0.1310844543000016 1104 6 0.1410601404407288 1104 7 0.12122375474322666 1104 8 0.12923014387381926 1104 9 0.22887544420542838 1104 10-11 0.2851272201123578 1104 12-13 0.30559503793595155 1104 14-15 0.34473520275245306 1104 16-17 0.36923453099903725 1104 18-19 0.3532971040469235 1104 20-21 0.31285639964838907 1104 22-23 0.30471040078111855 1104 24-25 0.29296988827617554 1104 26-27 0.35393690660087174 1104 28-29 0.4385160301390485 1104 30-31 0.2587035044335195 1104 32-33 0.3182583353189443 1104 34-35 0.43975741149063907 1104 36-37 0.4472516849333985 1104 38-39 0.35413404805204607 1104 40-41 0.2802268893875919 1104 42-43 0.4334777425332632 1104 44-45 0.4364153893254823 1104 46-47 0.3746806755759451 1104 48-49 0.555023924849344 1104 50-51 0.7030906156033012 1104 52-53 0.6354125966956943 1104 54-55 0.7363647224691618 1104 56-57 0.8219867415016893 1104 58-59 0.7864104040846556 1104 60-61 0.7495674509572439 1104 62-63 0.6983712520178287 1104 64-65 0.728077340687804 1104 66-67 0.7540294863352841 1104 68-69 0.8662232678089019 1104 70-71 0.8309242070390432 1104 72-73 0.8442455060240022 1104 74-75 0.8687726449813482 1104 76-77 0.8470672411432432 1104 78-79 0.9197512978158464 1104 80-81 0.8760230636541841 1104 82-83 1.0696646046252845 1104 84-85 1.2806172352244438 1104 86-87 1.3275923492286559 1104 88-89 1.2722205943451286 1104 90-91 1.292968409726683 1104 92-93 1.5693800894011432 1104 94-95 1.672189731696026 1104 96-97 1.6217270701368527 1104 98-99 1.5977301872237426 1104 100-101 1.8007482169669622 1105 1 0.2404517665417707 1105 2 0.25767214227319357 1105 3 0.18089945423965048 1105 4 0.1815849494576014 1105 5 0.09760419096728867 1105 6 0.17803187525581876 1105 7 0.19975537823477652 1105 8 0.25783941418537637 1105 9 0.3088914396681517 1105 10-11 0.20858435710748324 1105 12-13 0.2858894293743717 1105 14-15 0.40290079499622067 1105 16-17 0.2623639308947503 1105 18-19 0.36962057830324113 1105 20-21 0.41104580213160347 1105 22-23 0.29875980114729117 1105 24-25 0.3533130763304584 1105 26-27 0.36958584452740695 1105 28-29 0.34285192440172807 1105 30-31 0.38494525459501716 1105 32-33 0.5063162326212804 1105 34-35 0.6284790856475553 1105 36-37 0.5558470681166412 1105 38-39 0.43009380848601353 1105 40-41 0.4627879145608347 1105 42-43 0.4614836545817482 1105 44-45 0.5079761797069438 1105 46-47 0.4769253111934617 1105 48-49 0.4705488892276364 1105 50-51 0.5291494546864186 1105 52-53 0.5745704652044523 1105 54-55 0.5987896729516109 1105 56-57 0.5460062462086341 1105 58-59 0.6007262125415949 1105 60-61 0.6475947207817896 1105 62-63 0.530241340284725 1105 64-65 0.5789908724049582 1105 66-67 0.5932683776127519 1105 68-69 0.7535672064128107 1105 70-71 0.7199481048353569 1105 72-73 0.7778205572839738 1105 74-75 0.751452634230585 1105 76-77 0.6808465481894821 1105 78-79 0.9351508075845381 1105 80-81 0.8759676980955682 1105 82-83 0.9239875396010291 1105 84-85 1.1023772937002292 1105 86-87 1.2772899731270613 1105 88-89 1.1391577289531334 1105 90-91 1.4907702097389688 1105 92-93 1.7191052000560347 1105 94-95 1.79515188927137 1105 96-97 1.879737847907517 1105 98-99 1.9287094170805474 1105 100-101 1.8805390475418164 1106 1 -0.020756224251158528 1106 2 0.0770445885073272 1106 3 0.06642457415561154 1106 4 0.10467343881256141 1106 5 -0.03679995393255808 1106 6 0.014038847503293539 1106 7 0.08940033773068734 1106 8 0.14555739346945984 1106 9 -0.1744905123036702 1106 10-11 0.08387278621481187 1106 12-13 0.009964881381812063 1106 14-15 0.1415881432507149 1106 16-17 0.21483897575863864 1106 18-19 0.1479704368242878 1106 20-21 0.2029798283985471 1106 22-23 0.17915730679229114 1106 24-25 0.18410163374293376 1106 26-27 0.10077412181413337 1106 28-29 0.17986482614916355 1106 30-31 0.14364790506176206 1106 32-33 0.1301390221896881 1106 34-35 0.11499000960765926 1106 36-37 0.17150677540926296 1106 38-39 0.14686183192594626 1106 40-41 0.2162088987600015 1106 42-43 0.2968734747914752 1106 44-45 0.2071625362785312 1106 46-47 -0.41573005603817137 1106 48-49 -0.1213221430752256 1106 50-51 0.2652167174785305 1106 52-53 0.21036841592669475 1106 54-55 0.45599810487392745 1106 56-57 0.39991766502323145 1106 58-59 0.1979732304124866 1106 60-61 0.3235517527190481 1106 62-63 0.002513997728868844 1106 64-65 0.05341073146668407 1106 66-67 0.05368250714172973 1106 68-69 0.051234713285072786 1106 70-71 0.06709882948049994 1106 72-73 0.45937834480550066 1106 74-75 0.5370333244350256 1106 76-77 0.48507945936026786 1106 78-79 0.6986534007216285 1106 80-81 0.02241909533430686 1106 82-83 0.5862351453583656 1106 84-85 0.8492582342498913 1106 86-87 1.0560641826772539 1106 88-89 1.3038194882698484 1106 90-91 1.3096319671675793 1106 92-93 1.356265245061529 1106 94-95 1.4406085568906697 1106 96-97 1.3886879256200935 1106 98-99 1.3797221265848236 1106 100-101 1.3518240986621919 1107 1 0.06812208316085488 1107 2 0.12784639085259641 1107 3 0.08748253724439081 1107 4 0.056573209481307174 1107 5 0.01913518326238517 1107 6 -0.026887733858870888 1107 7 0.01134876011818875 1107 8 0.011077535689786089 1107 9 0.08851456779415656 1107 10-11 0.06870707402373455 1107 12-13 0.13390133582228714 1107 14-15 0.18214041722988128 1107 16-17 0.189898088635303 1107 18-19 0.09747239868284652 1107 20-21 0.08753715181891408 1107 22-23 0.1418814173631162 1107 24-25 0.17000198062418548 1107 26-27 0.2622122697853726 1107 28-29 0.10975413598963968 1107 30-31 0.07308676044264217 1107 32-33 0.1942619479647405 1107 34-35 0.32564397454638794 1107 36-37 0.32496620962000833 1107 38-39 0.2805467603757137 1107 40-41 0.20752318741280362 1107 42-43 0.2513423027824828 1107 44-45 0.2500188361964817 1107 46-47 0.2721875785369505 1107 48-49 0.3178340352381568 1107 50-51 0.3759184144375567 1107 52-53 0.3859035798884918 1107 54-55 0.3184702213637962 1107 56-57 0.16605812572293388 1107 58-59 0.2094050987222147 1107 60-61 0.40224983879028997 1107 62-63 0.6221842380338813 1107 64-65 0.3626249727500692 1107 66-67 0.31122296316053166 1107 68-69 0.49462867419558165 1107 70-71 0.636231886772439 1107 72-73 0.732607982961845 1107 74-75 0.6996005753244781 1107 76-77 0.6032029415693749 1107 78-79 0.8058423605163014 1107 80-81 0.9408061585063834 1107 82-83 1.0243623492146412 1107 84-85 1.07863135921054 1107 86-87 1.2230468102656396 1107 88-89 1.2060703760755338 1107 90-91 1.2278465456737315 1107 92-93 1.3250156880166557 1107 94-95 1.4519421921751814 1107 96-97 1.4464564437815035 1107 98-99 1.37536072860361 1107 100-101 1.6565995587339302 1108 1 0.11928655079685768 1108 2 0.1535813339803127 1108 3 0.18155512476521096 1108 4 0.15115203313729353 1108 5 0.07849473228883852 1108 6 0.152914091878678 1108 7 0.1280498870373492 1108 8 0.12204195620547864 1108 9 0.22170489647572822 1108 10-11 0.20262068613062922 1108 12-13 0.21729448272324703 1108 14-15 0.35032317727444706 1108 16-17 0.2585291142192432 1108 18-19 0.29668241960140307 1108 20-21 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-1.478670957387795 2316 88-89 -1.5587285361590801 2316 90-91 -1.4612122945152102 2316 92-93 -1.8198164390164635 2316 94-95 -1.68359439106435 2316 96-97 -1.8212176966964755 2316 98-99 -1.8709846080068893 2316 100-101 -2.267329473533458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 17.0 4 42.0 5 91.0 6 247.0 7 630.0 8 1413.0 9 2517.0 10 4052.0 11 6310.0 12 8834.0 13 12617.0 14 16645.0 15 21712.0 16 26667.0 17 32755.0 18 33008.0 19 30158.0 20 30683.0 21 32849.0 22 36058.0 23 40728.0 24 46933.0 25 54604.0 26 64167.0 27 77533.0 28 92235.0 29 108381.0 30 128208.0 31 151493.0 32 182225.0 33 222726.0 34 281641.0 35 362230.0 36 466785.0 37 503816.0 38 220463.0 39 7012.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46938462152271 18.933899315332656 15.082690840594498 23.514025222550135 2 16.303830449695145 20.187866402082 38.91224131476241 24.596061833460446 3 19.730227547777055 23.414111323225203 29.26529669710551 27.590364431892233 4 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22.895612260812477 24-25 23.086923574274014 25.94989204415444 28.290159559434958 22.673024822136586 26-27 22.56200504137851 26.38893695647213 28.093662821881317 22.955395180268045 28-29 22.717343025985755 26.65808872367734 28.038478633199386 22.58608961713751 30-31 22.777358732371443 26.140705657687274 27.99434054452461 23.087595065416675 32-33 22.472782744657575 26.219483259789794 27.995620937889438 23.312113057663193 34-35 23.239997877926672 26.199821618878953 27.78081159026875 22.77936891292562 36-37 23.342578349852225 25.819777200543697 27.79648145192108 23.041162997683003 38-39 22.731865114851388 26.168603261967743 27.84489685992116 23.25463476325971 40-41 23.198968578738437 25.98500594463652 27.91231649797964 22.903708978645405 42-43 23.039744193096855 26.00503017591712 27.640744122670764 23.314481508315264 44-45 22.932732933251334 26.25708515271798 27.809028883497973 23.001153030532713 46-47 23.28470191253296 26.542379428452602 27.17012765936868 23.00279099964576 48-49 23.12440229566824 26.43415791847162 27.26167994361177 23.17975984224837 50-51 23.31019680656689 26.65706909856106 27.11116021057423 22.92157388429782 52-53 23.645954108397422 26.3639597738184 27.04702021343863 22.943065904345545 54-55 23.044701349213497 26.544093510971617 27.27062796249357 23.140577177321315 56-57 23.12103218021987 26.686085382706075 27.176112258998963 23.016770178075085 58-59 23.08714271246034 26.280052664436543 27.12803474280739 23.504769880295733 60-61 22.776978151979815 26.528143335392677 27.46379619476184 23.231082317865663 62-63 22.908336697438088 26.619738979729373 27.386331673876253 23.085592648956286 64-65 22.579705106598446 26.54877984265298 27.264444259705712 23.607070791042858 66-67 22.572821696794048 26.613509231064064 27.259689760800608 23.55397931134128 68-69 22.97601438477893 26.28526324985845 27.369668926985096 23.36905343837753 70-71 22.816778719345706 26.403129404862675 27.078195979825832 23.701895895965784 72-73 22.860020734534487 26.462510087683505 27.048230461630535 23.629238716151477 74-75 22.74603594975161 26.826177968684245 26.85811832169997 23.56966775986417 76-77 22.862760746205492 26.491377497196293 27.04900396412405 23.596857792474168 78-79 22.951099963219416 26.626271012590813 27.092067714371627 23.330561309818147 80-81 22.95475276055053 26.80845801904291 26.84487433875431 23.39191488165225 82-83 22.92536190615423 26.808789973408388 26.80649198857478 23.459356131862602 84-85 22.89198311343413 26.72942951158646 26.85074313284189 23.527844242137512 86-87 23.001438857619597 26.738236734397763 26.642670592289413 23.61765381569322 88-89 22.835426365336208 26.86851999802762 26.480457253351915 23.81559638328426 90-91 22.683804689424985 26.88067528203516 26.71471178221429 23.72080824632557 92-93 22.751230213155317 26.531124661146244 26.845486013746584 23.872159111951856 94-95 22.559610955702674 26.477203585387254 26.88786481406038 24.07532064484969 96-97 22.7360565794984 26.28028803778417 26.84768558344584 24.13596979927159 98-99 22.48341434316263 26.512827375349573 26.499203510451135 24.50455477103666 100-101 22.498248583941386 26.277725086248267 26.959337673771838 24.264688656038512 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 430.0 1 314.5 2 292.0 3 374.5 4 310.0 5 303.0 6 557.0 7 1307.5 8 2034.5 9 2278.0 10 2012.0 11 1653.5 12 1672.0 13 1842.5 14 2063.0 15 2278.0 16 2472.0 17 2688.0 18 3001.5 19 3344.5 20 3762.5 21 4379.5 22 5210.5 23 6202.0 24 7616.5 25 9618.5 26 12271.0 27 15804.0 28 19480.0 29 23639.0 30 29125.0 31 36156.5 32 46068.0 33 50896.5 34 52378.0 35 57929.5 36 65176.0 37 72208.5 38 77912.5 39 83456.0 40 89661.5 41 98862.0 42 107436.5 43 113412.5 44 118714.5 45 125098.5 46 128964.0 47 131868.0 48 134168.5 49 132748.0 50 131275.0 51 127442.5 52 123639.0 53 123712.5 54 125425.5 55 118584.5 56 105810.5 57 95802.0 58 88301.5 59 78218.5 60 64075.5 61 53170.0 62 44828.0 63 34658.5 64 25118.5 65 18977.0 66 14087.5 67 10790.0 68 8291.5 69 6020.5 70 4668.5 71 3562.0 72 2747.5 73 2289.0 74 1804.0 75 1376.0 76 993.5 77 590.5 78 368.0 79 251.5 80 158.5 81 88.5 82 52.5 83 36.5 84 19.0 85 9.5 86 6.5 87 2.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.25406015792089E-4 2 0.05059706960149821 3 0.0 4 3.0225250658003708E-5 5 0.044310217464633435 6 6.0450501316007416E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0058939238783107225 26-27 0.002161105422047265 28-29 1.964641292770241E-4 30-31 0.0014054741555971724 32-33 6.347302638180779E-4 34-35 0.012286564392478508 36-37 0.02309209150271483 38-39 0.004579125474687562 40-41 0.0014356994062551761 42-43 0.0017832897888222187 44-45 0.00766210104180394 46-47 0.0 48-49 0.0 50-51 4.533787598700556E-4 52-53 0.0 54-55 0.0013903615302681706 56-57 0.0 58-59 0.022880514748108805 60-61 0.0 62-63 0.008508408060228043 64-65 0.015626454590187914 66-67 0.006226401635548764 68-69 0.016654113112560043 70-71 1.0578837730301298E-4 72-73 6.0450501316007416E-5 74-75 0.02309209150271483 76-77 0.051639840749199334 78-79 0.031449373309652855 80-81 0.02747475284812537 82-83 0.03767577494520162 84-85 0.016230959603347993 86-87 0.009430278205297156 88-89 0.08586993711938853 90-91 0.025298534800749103 92-93 0.025192746423446093 94-95 0.027187612966874334 96-97 0.03889989759685077 98-99 0.054239212305787654 100-101 0.013737376424062685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3308492.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.29957408490833 #Duplication Level Percentage of deduplicated Percentage of total 1 74.37629211208275 37.4109581525249 2 13.926372292039868 14.00981189674948 3 4.775297542372157 7.205862975300871 4 2.1719908020893754 4.370008490457361 5 1.2418763020517105 3.1232924529671005 6 0.73289910323114 2.2118707643842566 7 0.5244708713710916 1.8466463014934658 8 0.35344742540642 1.4222603967480267 9 0.2707702913738277 1.2257667297855057 >10 1.4544657508630927 13.415467077756634 >50 0.10617796815246522 3.6553953197044033 >100 0.059258545693865496 5.546048508115631 >500 0.0037317259726830257 1.3528933535725298 >1k 0.002828889042447346 2.7702419157160993 >5k 1.20378257125419E-4 0.4334756647238135 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7814 0.236180108641641 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6504 0.19658503027965613 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4726 0.14284453460972552 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3614 0.10923405587802539 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3504 0.10590927830564499 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3429 0.10364238450629472 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 3.0225250658003708E-5 0.0 0.0 0.0 16-17 0.0 3.0225250658003708E-5 0.0 1.5112625329001854E-5 0.0 18-19 0.0 3.0225250658003708E-5 0.0 9.067575197401113E-5 3.0225250658003708E-5 20-21 0.0 3.0225250658003708E-5 0.0 1.8135150394802226E-4 3.0225250658003708E-5 22-23 0.0 3.0225250658003708E-5 0.0 3.6270300789604447E-4 3.0225250658003708E-5 24-25 0.0 3.0225250658003708E-5 0.0 7.405186411210908E-4 3.0225250658003708E-5 26-27 0.0 3.0225250658003708E-5 0.0 0.0011938974009911463 3.0225250658003708E-5 28-29 0.0 3.0225250658003708E-5 0.0 0.0027353851845493353 3.0225250658003708E-5 30-31 0.0 3.0225250658003708E-5 0.0 0.00912802569871712 3.0225250658003708E-5 32-33 0.0 3.0225250658003708E-5 0.0 0.022940965249424815 3.0225250658003708E-5 34-35 0.0 3.0225250658003708E-5 0.0 0.04396262708206639 3.0225250658003708E-5 36-37 0.0 3.0225250658003708E-5 0.0 0.07976443648647177 3.0225250658003708E-5 38-39 0.0 3.0225250658003708E-5 0.0 0.13480461793469653 3.0225250658003708E-5 40-41 0.0 3.0225250658003708E-5 0.0 0.19215703105825857 3.0225250658003708E-5 42-43 0.0 3.0225250658003708E-5 0.0 0.2535445151446641 3.0225250658003708E-5 44-45 3.0225250658003708E-5 3.0225250658003708E-5 0.0 0.33365654201370293 3.0225250658003708E-5 46-47 3.0225250658003708E-5 3.0225250658003708E-5 0.0 0.4295612623515487 3.0225250658003708E-5 48-49 3.0225250658003708E-5 3.0225250658003708E-5 0.0 0.5313145686917182 3.0225250658003708E-5 50-51 3.0225250658003708E-5 3.0225250658003708E-5 0.0 0.6431177708756739 3.0225250658003708E-5 52-53 3.0225250658003708E-5 3.0225250658003708E-5 0.0 0.7550720993129196 3.0225250658003708E-5 54-55 3.0225250658003708E-5 3.0225250658003708E-5 0.0 0.8747036414172983 3.0225250658003708E-5 56-57 3.0225250658003708E-5 3.0225250658003708E-5 0.0 1.017064572016496 3.0225250658003708E-5 58-59 3.0225250658003708E-5 3.0225250658003708E-5 0.0 1.1736918209262708 3.0225250658003708E-5 60-61 3.0225250658003708E-5 4.5337875987005565E-5 0.0 1.345507258291693 3.0225250658003708E-5 62-63 3.0225250658003708E-5 6.0450501316007416E-5 0.0 1.515494067992306 3.0225250658003708E-5 64-65 3.0225250658003708E-5 6.0450501316007416E-5 0.0 1.6977221042094102 3.0225250658003708E-5 66-67 3.0225250658003708E-5 6.0450501316007416E-5 0.0 1.9103265173378081 3.0225250658003708E-5 68-69 6.0450501316007416E-5 6.0450501316007416E-5 0.0 2.1492420111639987 3.0225250658003708E-5 70-71 6.0450501316007416E-5 6.0450501316007416E-5 0.0 2.40283186418465 3.0225250658003708E-5 72-73 7.556312664500927E-5 6.0450501316007416E-5 0.0 2.6610461805559753 3.0225250658003708E-5 74-75 9.067575197401112E-5 6.0450501316007416E-5 0.0 2.9227364007529717 3.0225250658003708E-5 76-77 9.067575197401112E-5 6.0450501316007416E-5 0.0 3.213261510077703 3.0225250658003708E-5 78-79 9.067575197401112E-5 6.0450501316007416E-5 0.0 3.5429736568805366 3.0225250658003708E-5 80-81 9.067575197401112E-5 6.0450501316007416E-5 0.0 3.8935110013867344 3.0225250658003708E-5 82-83 9.067575197401112E-5 9.067575197401112E-5 0.0 4.248355444111699 3.0225250658003708E-5 84-85 1.0578837730301297E-4 9.067575197401112E-5 0.0 4.618403187917638 3.0225250658003708E-5 86-87 1.2090100263201483E-4 9.067575197401112E-5 0.0 5.000087653226908 3.0225250658003708E-5 88-89 1.2090100263201483E-4 1.0578837730301297E-4 0.0 5.4132668297218185 3.0225250658003708E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5190 0.0 45.965576 1 GTATCAA 8750 0.0 41.330723 1 ATCAACG 11425 0.0 31.432438 3 TATCAAC 11615 0.0 31.095015 2 TCAACGC 11640 0.0 30.851856 4 CAACGCA 11825 0.0 30.409355 5 AACGCAG 12290 0.0 29.284609 6 GTACATG 11645 0.0 25.791344 1 ACGCAGA 14125 0.0 25.379354 7 CGCAGAG 14340 0.0 24.93262 8 TACATGG 11980 0.0 24.633785 2 GTAAGGT 1835 0.0 23.55688 4 GTGGTAT 2805 0.0 23.549316 1 ACATGGG 12170 0.0 23.224098 3 GGACCGA 1220 0.0 22.962242 6 TAAGGTG 1930 0.0 22.889597 5 TGGTATC 2810 0.0 21.647116 2 GCAGAGT 16370 0.0 21.579746 9 GTATAGG 835 0.0 21.057734 1 AGGACCG 1475 0.0 20.933113 5 >>END_MODULE