FastQCFastQC Report
Thu 26 May 2016
SRR938430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3273759
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76180.23269886390537603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57710.176280538671295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42240.12902599122293362No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36880.11265337491244774No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36370.1110955326888754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47300.051.831161
GTATCAA84850.043.1438981
ATCAACG108850.033.404633
TATCAAC110000.033.0553972
TCAACGC110800.032.816734
CAACGCA112950.032.1896065
AACGCAG117850.030.8854066
ACGCAGA136650.026.3888177
CGCAGAG139500.025.8496918
GTACATG114200.025.8446371
TACATGG113900.025.6223142
ACATGGG119200.023.7254453
GCAGAGT153000.022.9456839
TAAGGTG19000.022.5127935
GAGTACT82400.020.780212-13
AGAGTAC139800.019.94599310-11
TGGTATC23150.019.9156482
GTAAGGT18000.019.8044344
CAGAGTA146000.019.79850810-11
GTACTTT85750.019.7213814-15
Produced by FastQC (version 0.11.5)