##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938430_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3273759 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.947490025991527 31.0 31.0 34.0 27.0 34.0 2 31.038192182136804 31.0 31.0 34.0 27.0 34.0 3 31.10188410325867 31.0 31.0 34.0 27.0 34.0 4 34.730768819574074 37.0 35.0 37.0 32.0 37.0 5 34.744261871445026 37.0 35.0 37.0 32.0 37.0 6 34.77223124854334 37.0 35.0 37.0 32.0 37.0 7 34.68792143832212 37.0 35.0 37.0 30.0 37.0 8 34.642139204504666 37.0 35.0 37.0 30.0 37.0 9 36.13213678832192 39.0 35.0 39.0 31.0 39.0 10-11 35.97546658138244 39.0 35.0 39.0 30.0 39.0 12-13 35.812236025926154 38.0 35.0 39.0 30.0 39.0 14-15 36.780295831183665 39.0 36.0 41.0 30.0 41.0 16-17 36.61800685389487 39.0 36.0 41.0 30.0 41.0 18-19 36.593725591896046 39.0 36.0 40.5 30.0 41.0 20-21 36.67793979336903 39.0 36.0 41.0 30.0 41.0 22-23 36.59501371359346 39.0 36.0 41.0 30.0 41.0 24-25 36.47109011384161 39.0 36.0 41.0 29.5 41.0 26-27 36.26941353960386 39.0 35.5 41.0 29.0 41.0 28-29 36.07673671152947 39.0 35.0 40.5 27.5 41.0 30-31 35.855610935319305 38.5 35.0 40.0 27.0 41.0 32-33 35.57618474664751 38.0 34.5 40.0 27.0 41.0 34-35 35.31933551003601 38.0 34.0 40.0 26.0 41.0 36-37 35.02283185781238 38.0 34.0 40.0 25.0 41.0 38-39 34.75738012480454 38.0 33.5 40.0 24.5 41.0 40-41 34.68547119687185 38.0 33.0 40.0 24.0 41.0 42-43 34.49306897667177 38.0 33.0 40.0 24.0 41.0 44-45 34.197564787145296 38.0 33.0 40.0 23.5 41.0 46-47 33.83529835274985 38.0 33.0 40.0 22.0 41.0 48-49 33.468101653176056 38.0 32.5 40.0 19.5 41.0 50-51 32.72835523323494 36.5 31.5 39.5 18.0 40.5 52-53 32.783894599449745 37.0 31.5 39.0 18.0 40.0 54-55 32.88805254143631 37.0 31.5 40.0 16.5 41.0 56-57 32.56504831296378 37.0 31.0 40.0 13.5 41.0 58-59 32.20593177445255 36.0 31.0 39.0 10.5 41.0 60-61 31.732021049808495 36.0 30.5 39.0 8.0 40.5 62-63 31.181361547994214 35.0 29.5 39.0 6.5 40.0 64-65 30.65464470658958 35.0 29.0 38.5 2.0 40.0 66-67 30.0889975407475 34.5 28.0 38.0 2.0 40.0 68-69 29.499316534906818 34.0 28.0 37.5 2.0 39.5 70-71 28.84351337407549 34.0 26.0 37.0 2.0 39.0 72-73 28.265998657812013 34.0 26.0 36.0 2.0 39.0 74-75 27.62153903204237 33.0 25.5 36.0 2.0 38.5 76-77 26.854193604355117 32.5 24.0 35.0 2.0 37.5 78-79 26.04116430073197 32.0 21.5 35.0 2.0 37.0 80-81 25.356742661875842 31.0 19.5 35.0 2.0 36.0 82-83 24.67011056708817 31.0 17.0 34.0 2.0 36.0 84-85 23.985165371061214 31.0 9.0 34.0 2.0 35.0 86-87 23.286504596092747 30.0 4.5 34.0 2.0 35.0 88-89 22.46146570349253 29.5 2.0 34.0 2.0 35.0 90-91 21.600367192575874 29.0 2.0 34.0 2.0 35.0 92-93 20.7163314404023 28.5 2.0 34.0 2.0 35.0 94-95 19.729412733191417 26.5 2.0 33.5 2.0 35.0 96-97 18.46589287727044 25.0 2.0 33.0 2.0 34.5 98-99 16.977190746172823 20.0 2.0 32.0 2.0 34.0 100-101 13.668158376960552 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6357006395253713 1101 2 0.6879057211506563 1101 3 0.7154089211182146 1101 4 0.6498792018754003 1101 5 0.5425988660055054 1101 6 0.6460067898861581 1101 7 0.5792427183388611 1101 8 0.6707867591622758 1101 9 0.7174431231167091 1101 10-11 0.7366097322309102 1101 12-13 0.8488898617238618 1101 14-15 1.0240541051618308 1101 16-17 1.0207634415311375 1101 18-19 0.9619516327883204 1101 20-21 0.9597989081335356 1101 22-23 1.0583672617659161 1101 24-25 1.0197698584374137 1101 26-27 1.1091699540768971 1101 28-29 1.143289115825155 1101 30-31 1.2238632801142302 1101 32-33 1.347671749630834 1101 34-35 1.303762128799498 1101 36-37 1.2335790508071298 1101 38-39 1.1886060568217687 1101 40-41 1.2120241844510673 1101 42-43 1.2018354682738277 1101 44-45 1.2000745311713104 1101 46-47 1.3952606569742443 1101 48-49 1.453758560784209 1101 50-51 1.2865995244038615 1101 52-53 1.4237413875018987 1101 54-55 1.5934054746818234 1101 56-57 1.4078485532711653 1101 58-59 1.2993804019438286 1101 60-61 1.4379745748471642 1101 62-63 1.4011269366827328 1101 64-65 1.281241754227466 1101 66-67 1.3549842642443295 1101 68-69 1.4470798958063043 1101 70-71 1.5002527517820248 1101 72-73 1.5051403866275024 1101 74-75 1.4606969209818992 1101 76-77 1.6926458244709508 1101 78-79 1.6453435203885256 1101 80-81 1.8590405240641168 1101 82-83 1.8409694697883587 1101 84-85 1.9251959246636048 1101 86-87 2.092342960916202 1101 88-89 2.2101361201274337 1101 90-91 2.2942354354208554 1101 92-93 2.2756520915096097 1101 94-95 2.1267318462246934 1101 96-97 2.100962639268559 1101 98-99 1.8171055629408066 1101 100-101 1.775634047327575 1102 1 0.6878566612025345 1102 2 0.735131852680464 1102 3 0.637985007256006 1102 4 0.6402909808063697 1102 5 0.5490639577608079 1102 6 0.6291262125155654 1102 7 0.5169509963362273 1102 8 0.5827182191994567 1102 9 0.6457996546116291 1102 10-11 0.6612651790746824 1102 12-13 0.773031328222963 1102 14-15 0.7570019202002669 1102 16-17 0.8725059795989836 1102 18-19 0.8455890992398523 1102 20-21 0.6123802569701908 1102 22-23 0.8002076007986858 1102 24-25 0.8404390834430586 1102 26-27 0.6923352147142978 1102 28-29 0.5660409225859837 1102 30-31 0.6265421028554314 1102 32-33 0.5972793762824935 1102 34-35 0.8295284411837827 1102 36-37 0.6716532399947042 1102 38-39 0.6614746142278065 1102 40-41 0.6713930272916215 1102 42-43 0.8980297243006632 1102 44-45 0.9264941714445385 1102 46-47 0.7741841426846818 1102 48-49 0.5455564810766163 1102 50-51 0.7277890132778637 1102 52-53 0.7136311582424852 1102 54-55 0.5445817027440043 1102 56-57 0.5283798182614348 1102 58-59 0.6392463369202588 1102 60-61 0.5287497376835937 1102 62-63 0.44389107262884764 1102 64-65 0.5544956977827873 1102 66-67 0.46710938367092325 1102 68-69 0.43033098501648226 1102 70-71 0.5509669327237212 1102 72-73 0.6377655020897279 1102 74-75 0.5758685583056327 1102 76-77 0.7230595657642063 1102 78-79 0.5698987678245722 1102 80-81 0.599171080621776 1102 82-83 0.7965801906294381 1102 84-85 0.6699773789524563 1102 86-87 0.7605412447411872 1102 88-89 1.0637062815168647 1102 90-91 1.116658992489917 1102 92-93 1.0222461045206686 1102 94-95 0.9570868157585224 1102 96-97 1.1164976604977497 1102 98-99 1.1933588484110018 1102 100-101 0.9932551191341776 1103 1 0.5225856315897843 1103 2 0.5861656986987533 1103 3 0.5989822575483608 1103 4 0.5312092835538778 1103 5 0.5972808619022274 1103 6 0.5718990594638313 1103 7 0.5839343917798132 1103 8 0.5007532748592496 1103 9 0.4356310997357653 1103 10-11 0.5731284292463599 1103 12-13 0.599230027403415 1103 14-15 0.7911819068108841 1103 16-17 0.8737847708386113 1103 18-19 0.7349938655913277 1103 20-21 0.6369061933888318 1103 22-23 0.7961309748460792 1103 24-25 0.9108471626606871 1103 26-27 0.8640595332972723 1103 28-29 0.855721856892778 1103 30-31 0.8643357378233603 1103 32-33 0.9464456047773524 1103 34-35 0.9875764360808788 1103 36-37 1.0248847450741536 1103 38-39 0.9233861830169445 1103 40-41 0.9572991234825423 1103 42-43 0.9069815024162082 1103 44-45 1.1651182736712116 1103 46-47 1.152348006488431 1103 48-49 1.0105483258134313 1103 50-51 1.2173518566920265 1103 52-53 1.1361450959368469 1103 54-55 1.2828608224927578 1103 56-57 1.1637673393200174 1103 58-59 1.1796474053890336 1103 60-61 1.2719654973106849 1103 62-63 1.2926619887479198 1103 64-65 1.1557573931389342 1103 66-67 1.3539807029080535 1103 68-69 1.3650419017676754 1103 70-71 1.3877454936238536 1103 72-73 1.2661210121839623 1103 74-75 1.3532994061752568 1103 76-77 1.3730342810961993 1103 78-79 1.427866359392901 1103 80-81 1.3453676057038813 1103 82-83 1.461677962801172 1103 84-85 1.4991889761868507 1103 86-87 1.74066826188783 1103 88-89 1.8379643824275895 1103 90-91 1.8530003872266825 1103 92-93 1.8681848983845377 1103 94-95 1.8323622414555985 1103 96-97 1.7268070050023105 1103 98-99 1.8259503544865012 1103 100-101 1.4493996765614998 1104 1 0.5031442949745824 1104 2 0.46045897832879845 1104 3 0.45261562071685546 1104 4 0.5651616964725008 1104 5 0.46271309879340095 1104 6 0.452548160679612 1104 7 0.4227322212718647 1104 8 0.4356949098260543 1104 9 0.5842307118786749 1104 10-11 0.5360829492732151 1104 12-13 0.5046921926902073 1104 14-15 0.5728095080639619 1104 16-17 0.6320593661992433 1104 18-19 0.6047503360918043 1104 20-21 0.7324621529621922 1104 22-23 0.6766695927322601 1104 24-25 0.5321000529418853 1104 26-27 0.5967355607456213 1104 28-29 0.5121876956337346 1104 30-31 0.547342749086134 1104 32-33 0.5613603880995015 1104 34-35 0.4199032989137308 1104 36-37 0.5148522061019705 1104 38-39 0.6168979679054374 1104 40-41 0.5939418287857379 1104 42-43 0.5588457337878765 1104 44-45 0.6512612400320705 1104 46-47 0.7715940160356851 1104 48-49 0.674793216542156 1104 50-51 0.4628113181673754 1104 52-53 0.5348617178507737 1104 54-55 0.7661806367688868 1104 56-57 0.8104747496280638 1104 58-59 0.7751059345156079 1104 60-61 0.8925284494998387 1104 62-63 0.9805708236848965 1104 64-65 0.8842210688307333 1104 66-67 0.9470053355626362 1104 68-69 0.9020640730214922 1104 70-71 0.9669438601488061 1104 72-73 1.1451315104311455 1104 74-75 0.9359940805990661 1104 76-77 0.8243377046808895 1104 78-79 1.0412469628090264 1104 80-81 1.0708053434528146 1104 82-83 1.0754650702823376 1104 84-85 0.9752094319189268 1104 86-87 1.0344933159640597 1104 88-89 1.0765435761323694 1104 90-91 1.0020773619413852 1104 92-93 1.1777997451064017 1104 94-95 1.0639945044525376 1104 96-97 1.096628739916909 1104 98-99 0.8652782242560271 1104 100-101 0.6180969988558243 1105 1 0.4891625124673844 1105 2 0.5518364613931155 1105 3 0.5199091701203464 1105 4 0.46255080894260203 1105 5 0.345079224568579 1105 6 0.4623262782053601 1105 7 0.46434941761118154 1105 8 0.4256142415648796 1105 9 0.5007900834324559 1105 10-11 0.5348357778540489 1105 12-13 0.5437268768200809 1105 14-15 0.7044655347313906 1105 16-17 0.6522384261078713 1105 18-19 0.7059265244538082 1105 20-21 0.6282920550002018 1105 22-23 0.7348763261868498 1105 24-25 0.7540500192853585 1105 26-27 0.767175279749388 1105 28-29 0.7985075687414493 1105 30-31 0.7536712511556516 1105 32-33 0.8066683453628656 1105 34-35 0.8757551493068618 1105 36-37 0.9155096721231928 1105 38-39 0.8858858014273423 1105 40-41 0.8746971851869247 1105 42-43 1.0610213242093707 1105 44-45 1.0863060139604386 1105 46-47 0.9405822971178495 1105 48-49 0.9685741877121146 1105 50-51 0.8491063069246607 1105 52-53 0.8967883931583387 1105 54-55 1.0474616079388426 1105 56-57 1.0466869397373415 1105 58-59 1.0955352701213528 1105 60-61 1.1277412375496247 1105 62-63 1.0512166889441907 1105 64-65 1.0363527088347482 1105 66-67 1.1289198730359615 1105 68-69 0.9416669796568442 1105 70-71 1.1223845816875517 1105 72-73 1.0630383069022216 1105 74-75 1.1013158585232468 1105 76-77 1.23173748698758 1105 78-79 1.228301461326705 1105 80-81 1.1181000207081624 1105 82-83 1.230942581274416 1105 84-85 1.230169742275116 1105 86-87 1.2789970969531304 1105 88-89 1.3227678682710646 1105 90-91 1.253007929232968 1105 92-93 1.1773155744176584 1105 94-95 1.189776213201803 1105 96-97 1.173227425163887 1105 98-99 1.0801011515149526 1105 100-101 0.8801249122940913 1106 1 0.41155688687562275 1106 2 0.4890504125684991 1106 3 0.33360815193403326 1106 4 0.45480167780054614 1106 5 0.46629140431329574 1106 6 0.22928990417360495 1106 7 0.2734382546479921 1106 8 0.4202219841637884 1106 9 0.5578230213663602 1106 10-11 0.26802439518288423 1106 12-13 0.3001204807051465 1106 14-15 0.5565422948535499 1106 16-17 0.4325589869251161 1106 18-19 0.08930877348240074 1106 20-21 0.42764610091411015 1106 22-23 0.3924627132830878 1106 24-25 0.4885834524050665 1106 26-27 0.3230046703266751 1106 28-29 0.3064511744527323 1106 30-31 0.3906354320129637 1106 32-33 0.5277588885079467 1106 34-35 0.3790350529106945 1106 36-37 0.387770574639724 1106 38-39 0.4257241144802961 1106 40-41 0.6873504820708334 1106 42-43 0.24721733490819275 1106 44-45 0.5079598046253722 1106 46-47 0.2291838748290047 1106 48-49 0.5865507144999071 1106 50-51 0.3821222062523262 1106 52-53 0.5357712463838595 1106 54-55 0.41648478544434653 1106 56-57 -0.1439532758124784 1106 58-59 -0.4738605434864951 1106 60-61 0.2600348679850768 1106 62-63 0.27607204903207716 1106 64-65 0.418996130098094 1106 66-67 0.0030817447118920427 1106 68-69 -0.12147061597372044 1106 70-71 -0.14484937710175316 1106 72-73 0.3861697004183071 1106 74-75 0.3846286937394545 1106 76-77 0.21006678625400355 1106 78-79 0.3301911219874576 1106 80-81 0.5227673576901815 1106 82-83 0.4723061544228422 1106 84-85 0.44493314530438965 1106 86-87 0.4957883912353829 1106 88-89 0.39286695108861025 1106 90-91 0.6671440266630562 1106 92-93 0.6854311781791047 1106 94-95 0.6738629255051691 1106 96-97 0.7242092627317049 1106 98-99 0.7490263278853888 1106 100-101 0.24091325512843476 1107 1 0.449164390963805 1107 2 0.3654661354199291 1107 3 0.33232491651522267 1107 4 0.3026985344415749 1107 5 0.35370358407224955 1107 6 0.26306662878143783 1107 7 0.20778126839640265 1107 8 0.27415269702188283 1107 9 0.2776272427945017 1107 10-11 0.369449202886841 1107 12-13 0.39263266954895215 1107 14-15 0.5022905838729912 1107 16-17 0.4148946268015621 1107 18-19 0.374696166735383 1107 20-21 0.4410273316088009 1107 22-23 0.49191156556337745 1107 24-25 0.4006099044673306 1107 26-27 0.2939369276096997 1107 28-29 0.40681040466289886 1107 30-31 0.43810378644334236 1107 32-33 0.48452445388860355 1107 34-35 0.6073582162563369 1107 36-37 0.4455180611638596 1107 38-39 0.5947913272475418 1107 40-41 0.5353987529205781 1107 42-43 0.5593009458342451 1107 44-45 0.6955866140251743 1107 46-47 0.5237848226819537 1107 48-49 0.5594835320155056 1107 50-51 0.47900458769500887 1107 52-53 0.49094506989545295 1107 54-55 0.5237276841179508 1107 56-57 0.5300292207049964 1107 58-59 0.47330157570677045 1107 60-61 0.5396287750773219 1107 62-63 0.5666908031959679 1107 64-65 0.5020846771273497 1107 66-67 0.6446275323686095 1107 68-69 0.5674337508120573 1107 70-71 0.6111560889511836 1107 72-73 0.6819384303559239 1107 74-75 0.8808925481395526 1107 76-77 0.8566849499024833 1107 78-79 0.9202428299397347 1107 80-81 0.9692102593920815 1107 82-83 0.9689182615029708 1107 84-85 0.8867465575749947 1107 86-87 0.6495495201797254 1107 88-89 0.7241896299087891 1107 90-91 0.8678833065370775 1107 92-93 0.8125623331666887 1107 94-95 0.5658572777551854 1107 96-97 0.6646996933537146 1107 98-99 0.5578564260017949 1107 100-101 0.19057938679823216 1108 1 0.4606247749643515 1108 2 0.48072320005053726 1108 3 0.4802971799020952 1108 4 0.4493659407870112 1108 5 0.4079039650626797 1108 6 0.42870754982229897 1108 7 0.4002137847198952 1108 8 0.15246302489287444 1108 9 0.3668369440726593 1108 10-11 0.4352608087149292 1108 12-13 0.443223387407194 1108 14-15 0.5591947449683303 1108 16-17 0.41654415515578336 1108 18-19 0.5237675151217402 1108 20-21 0.4817514665114615 1108 22-23 0.5711139829051106 1108 24-25 0.49072322616021324 1108 26-27 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2316 68-69 -0.774320130557264 2316 70-71 -1.174586003411786 2316 72-73 -0.940333480017383 2316 74-75 -0.8469600258193815 2316 76-77 -0.707465663734137 2316 78-79 -0.677472898175882 2316 80-81 -0.6246214410988351 2316 82-83 -0.4327978939658159 2316 84-85 -0.5536638786864287 2316 86-87 -0.6876180785511785 2316 88-89 -0.7420143211839125 2316 90-91 -0.5930050597687249 2316 92-93 -0.9331908447239634 2316 94-95 -0.7579492473808429 2316 96-97 -1.0031981172545699 2316 98-99 -1.117805515700617 2316 100-101 -0.9509528262126814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12098.0 3 4612.0 4 4878.0 5 5506.0 6 6519.0 7 8016.0 8 10230.0 9 12579.0 10 15837.0 11 19048.0 12 22842.0 13 26077.0 14 29304.0 15 33808.0 16 37392.0 17 42158.0 18 41615.0 19 40641.0 20 43430.0 21 47454.0 22 52317.0 23 57838.0 24 64261.0 25 71459.0 26 80126.0 27 90616.0 28 102714.0 29 117206.0 30 134679.0 31 156133.0 32 182680.0 33 217424.0 34 264664.0 35 321395.0 36 374183.0 37 358293.0 38 156742.0 39 6985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59163616478331 18.086186219544402 14.444137411022787 23.878040204649505 2 15.74519895616128 19.350990005662936 39.98040433928365 24.923406698892137 3 19.651433179950242 23.088571324665768 28.7326045879239 28.52739090746009 4 13.13989502387238 16.003022021514788 35.87562523265715 34.981457721955685 5 14.415336568643571 36.78754354617958 33.572813124462456 15.224306760714393 6 33.618330363446866 34.95783576273103 17.030133904692622 14.39369996912948 7 30.111645140975025 31.468669721728986 19.71572458726255 18.703960550033443 8 27.021232906849974 34.611398203783104 19.207487740844133 19.15988114852279 9 26.617588234377372 16.660855524410007 19.34639775898723 37.37515848222539 10-11 25.550668398927456 26.0400020229742 26.90354003678329 21.505789541315046 12-13 26.418894452092363 23.198614512780004 27.155702300538277 23.226788734589356 14-15 22.94729743397474 24.86597248895314 25.285082512667874 26.901647564404247 16-17 23.06571629145892 27.993800306623573 25.944693173997035 22.99579022792047 18-19 23.064299118311517 26.54689682505972 27.96179095246375 22.427013104165017 20-21 23.40402188867775 25.83731282436188 28.2319446829864 22.52672060397397 22-23 23.12487250852044 25.705888330069804 28.103331700933065 23.065907460476687 24-25 22.984054916875674 25.869186833313186 28.194843221379646 22.9519150284315 26-27 22.544508810077915 26.27446246376568 27.995632780732894 23.18539594542351 28-29 22.700443319330184 26.606127261709368 27.983146103468474 22.710283315491974 30-31 22.762485257843714 26.097291660745352 27.877108101607572 23.26311497980336 32-33 22.483896885055373 26.134888742809487 27.892349259349924 23.488865112785216 34-35 23.24207427183294 26.228667789450782 27.600991741699755 22.928266197016523 36-37 23.353640182196287 25.766671403403425 27.65711832215616 23.22257009224413 38-39 22.771165341726665 26.165677054601623 27.619823976512688 23.443333627159028 40-41 23.21256192519313 26.014943775613915 27.731212466599846 23.04128183259311 42-43 23.02643096300655 25.961957084629127 27.54018905054027 23.471422901824056 44-45 22.897661040227373 26.267949701734366 27.65928069227931 23.175108565758958 46-47 23.21092334094275 26.57933024425227 27.06024319066891 23.149503224136076 48-49 23.144690113415052 26.410982548592816 27.140153511284225 23.30417382670791 50-51 23.288543755756532 26.65472719191191 27.021281795534076 23.035447256797482 52-53 23.524079197225678 26.389849479814558 27.004980680921157 23.08109064203861 54-55 22.95210248896715 26.545347567640555 27.245810632717117 23.256739310675183 56-57 23.04313638277086 26.711238016568313 27.124391854779535 23.12123374588129 58-59 23.09708784429237 26.193225611035807 27.155217315728486 23.554469228943333 60-61 22.739488915775535 26.475313919246684 27.456735911915803 23.32846125306198 62-63 22.87325960643485 26.53357314991112 27.45492518510893 23.1382420585451 64-65 22.61241813683195 26.48243415778061 27.32136122924314 23.583786476144304 66-67 22.573517821817262 26.536796483901043 27.29787868383335 23.591807010448345 68-69 22.9967496364725 26.16107323093466 27.477623202219043 23.36455393037379 70-71 22.854525392754415 26.310241808186476 27.126096772463892 23.709136026595218 72-73 22.947142949452658 26.368384237824266 27.090030840420948 23.59444197230213 74-75 22.85226987173661 26.734083936027936 27.027455988463707 23.38619020377175 76-77 23.0244602096764 26.439052629890686 26.99257876612154 23.54390839431138 78-79 23.082621382875463 26.567096700780723 27.042814779854762 23.307467136489056 80-81 23.171362600573968 26.703428278428426 26.758521819073543 23.366687301924056 82-83 23.124750462112857 26.718462611396614 26.78033731565508 23.37644961083545 84-85 23.144338045551013 26.647243501576632 26.804471859194017 23.40394659367833 86-87 23.288050156536798 26.608429510634068 26.64319340255869 23.460326930270444 88-89 23.158233571287475 26.6944856125258 26.5389168607775 23.608363955409224 90-91 23.144840963669136 26.713009802246944 26.604870047426875 23.537279186657038 92-93 23.269714292523137 26.313144267098842 26.778596908297015 23.638544532081003 94-95 23.07556336684382 26.284799984355693 26.85372407021421 23.785912578586277 96-97 23.36669133083697 26.012637853639664 26.79685457729162 23.823816238231746 98-99 23.214913398940173 26.162806417862544 26.443703620760058 24.17857656243722 100-101 23.80175791105851 25.58928860874857 26.704130846323466 23.904822633869454 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 442.0 1 311.5 2 295.5 3 407.0 4 349.0 5 319.0 6 553.5 7 1256.5 8 2040.0 9 2208.0 10 1899.0 11 1674.5 12 1735.5 13 1895.0 14 2028.5 15 2233.5 16 2460.5 17 2683.5 18 2949.0 19 3265.0 20 3715.0 21 4311.0 22 5139.5 23 6256.0 24 7622.5 25 9456.5 26 12165.0 27 15539.5 28 19012.0 29 23187.0 30 28352.0 31 35204.5 32 44418.5 33 49463.5 34 52012.0 35 57055.0 36 64109.5 37 71458.0 38 77258.5 39 82393.5 40 88409.5 41 96912.5 42 104476.0 43 110263.0 44 115581.5 45 122294.0 46 126467.5 47 128871.0 48 130430.0 49 129347.5 50 127967.0 51 124392.5 52 121285.5 53 121063.0 54 122077.0 55 116082.5 56 104894.5 57 95985.0 58 89585.0 59 80336.5 60 66041.0 61 54956.0 62 46514.0 63 36248.5 64 27030.5 65 20917.0 66 15900.0 67 12198.5 68 9356.0 69 6926.5 70 5520.5 71 4233.0 72 3178.0 73 2590.5 74 1987.5 75 1459.5 76 1050.5 77 703.0 78 462.0 79 316.0 80 189.5 81 106.0 82 66.5 83 43.5 84 31.0 85 23.0 86 17.0 87 11.0 88 11.0 89 11.5 90 6.5 91 5.0 92 4.5 93 3.5 94 2.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18107624904582165 2 0.04905675707955289 3 0.047651644485742536 4 0.05428011041741313 5 0.05941182597741618 6 0.0619776837574177 7 0.03537218225287811 8 0.033692156325496166 9 0.04001516299764277 10-11 0.04122172707276254 12-13 0.03861005040383242 14-15 0.03390597780716296 16-17 0.031049933730613648 18-19 0.03934315262668999 20-21 0.04780437411550453 22-23 0.04525378929847921 24-25 0.06486427375991942 26-27 0.05052296152526804 28-29 0.04276429633335869 30-31 0.051317155600030424 32-33 0.04783492004145693 34-35 0.046918542262884964 36-37 0.04471923559431223 38-39 0.025765488540848605 40-41 0.02313853890894229 42-43 0.020343586684297775 44-45 0.005101169634050644 46-47 0.012264189269888223 48-49 0.011729635565721241 50-51 0.009285961489529315 52-53 0.009499782971196108 54-55 0.01078271186119687 56-57 0.008583405192624136 58-59 0.010446706675720478 60-61 0.010553617416553876 62-63 0.004459705189050263 64-65 0.007101927783932782 66-67 0.004001516299764277 68-69 0.008522313340719338 70-71 0.02106141594417915 72-73 0.01892320112751122 74-75 0.018678833719892023 76-77 0.011653270750840243 78-79 0.00554408556036043 80-81 0.00719356556178998 82-83 0.006353552598099005 84-85 0.005391355930598435 86-87 0.007560116673218768 88-89 0.028392438172754927 90-91 0.03488344743763973 92-93 0.021641788537274737 94-95 0.030622290767280062 96-97 0.034532169289187134 98-99 0.020633772980845566 100-101 0.12123678010507188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3273759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.3397239820791 #Duplication Level Percentage of deduplicated Percentage of total 1 76.83722776954312 40.98476520775574 2 12.654161252992813 13.499389369187137 3 4.30247868973368 6.884790772475155 4 1.963051187746712 4.188344340684086 5 1.0922949296548035 2.9131355027405865 6 0.6779139359225497 2.169584533542819 7 0.45583136622254605 1.7019743479679235 8 0.3195942642778374 1.363765587426839 9 0.24944510236081566 1.1974799617746639 >10 1.300503760987773 12.542393276755186 >50 0.0901708229735396 3.2972260994466907 >100 0.05139809443595725 5.039068878083304 >500 0.0032234378292041793 1.1926306895779268 >1k 0.0025902625392134446 2.614367808526088 >5k 1.1512277952059754E-4 0.4110836240558519 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7618 0.23269886390537603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5771 0.176280538671295 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4224 0.12902599122293362 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3688 0.11265337491244774 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3637 0.1110955326888754 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 3.054592595239906E-5 0.0 0.0 0.0 0.0 12-13 6.109185190479812E-5 0.0 0.0 6.109185190479812E-5 0.0 14-15 6.109185190479812E-5 0.0 0.0 6.109185190479812E-5 0.0 16-17 6.109185190479812E-5 0.0 0.0 6.109185190479812E-5 0.0 18-19 6.109185190479812E-5 0.0 0.0 9.163777785719718E-5 0.0 20-21 9.163777785719718E-5 0.0 0.0 1.2218370380959623E-4 3.054592595239906E-5 22-23 9.163777785719718E-5 0.0 0.0 2.443674076191925E-4 6.109185190479812E-5 24-25 9.163777785719718E-5 0.0 0.0 6.261914820241808E-4 6.109185190479812E-5 26-27 1.2218370380959623E-4 0.0 0.0 0.0012523829640483616 6.109185190479812E-5 28-29 1.527296297619953E-4 0.0 0.0 0.00293240889143031 6.109185190479812E-5 30-31 1.527296297619953E-4 0.0 0.0 0.00946923704524371 6.109185190479812E-5 32-33 1.527296297619953E-4 0.0 0.0 0.023031628168108893 6.109185190479812E-5 34-35 1.6800259273819484E-4 0.0 0.0 0.04595634559538439 6.109185190479812E-5 36-37 1.8327555571439436E-4 0.0 0.0 0.08365001822064483 6.109185190479812E-5 38-39 1.8327555571439436E-4 0.0 0.0 0.13887705234258232 6.109185190479812E-5 40-41 1.8327555571439436E-4 0.0 0.0 0.19996890424738045 6.109185190479812E-5 42-43 1.985485186905939E-4 0.0 0.0 0.2609232994853928 6.109185190479812E-5 44-45 2.4436740761919247E-4 0.0 0.0 0.3415645439997263 6.109185190479812E-5 46-47 2.749133335715916E-4 0.0 0.0 0.4391587774176413 6.109185190479812E-5 48-49 2.749133335715916E-4 0.0 0.0 0.5449240460278231 6.109185190479812E-5 50-51 2.749133335715916E-4 0.0 0.0 0.6569665024212228 6.109185190479812E-5 52-53 2.749133335715916E-4 0.0 0.0 0.7677871217765266 6.109185190479812E-5 54-55 2.749133335715916E-4 0.0 0.0 0.8908719304017186 9.163777785719718E-5 56-57 3.054592595239906E-4 0.0 0.0 1.0379352908995438 9.163777785719718E-5 58-59 3.054592595239906E-4 0.0 0.0 1.195781363258566 9.163777785719718E-5 60-61 3.054592595239906E-4 0.0 0.0 1.3684422097045017 9.163777785719718E-5 62-63 3.054592595239906E-4 0.0 0.0 1.5365211672575776 1.2218370380959623E-4 64-65 3.054592595239906E-4 0.0 0.0 1.7170934085251846 1.2218370380959623E-4 66-67 3.054592595239906E-4 0.0 0.0 1.9334654750090035 1.2218370380959623E-4 68-69 3.054592595239906E-4 0.0 0.0 2.1692189315096195 1.527296297619953E-4 70-71 3.054592595239906E-4 0.0 0.0 2.4216046446913166 1.527296297619953E-4 72-73 3.054592595239906E-4 0.0 0.0 2.684024694548377 1.8327555571439436E-4 74-75 3.054592595239906E-4 0.0 0.0 2.9512404547799638 2.2909444464299294E-4 76-77 3.054592595239906E-4 0.0 0.0 3.2416252998464454 2.4436740761919247E-4 78-79 3.054592595239906E-4 0.0 0.0 3.565977214571995 2.4436740761919247E-4 80-81 3.054592595239906E-4 0.0 0.0 3.9111309048711282 2.4436740761919247E-4 82-83 3.054592595239906E-4 0.0 0.0 4.267739317402411 2.4436740761919247E-4 84-85 3.054592595239906E-4 0.0 0.0 4.624026997711193 3.054592595239906E-4 86-87 3.054592595239906E-4 0.0 0.0 4.997084391367844 3.2073222250019015E-4 88-89 3.054592595239906E-4 0.0 0.0 5.399679695420463 3.360051854763897E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4730 0.0 51.83116 1 GTATCAA 8485 0.0 43.143898 1 ATCAACG 10885 0.0 33.40463 3 TATCAAC 11000 0.0 33.055397 2 TCAACGC 11080 0.0 32.81673 4 CAACGCA 11295 0.0 32.189606 5 AACGCAG 11785 0.0 30.885406 6 ACGCAGA 13665 0.0 26.388817 7 CGCAGAG 13950 0.0 25.849691 8 GTACATG 11420 0.0 25.844637 1 TACATGG 11390 0.0 25.622314 2 ACATGGG 11920 0.0 23.725445 3 GCAGAGT 15300 0.0 22.945683 9 TAAGGTG 1900 0.0 22.512793 5 GAGTACT 8240 0.0 20.7802 12-13 AGAGTAC 13980 0.0 19.945993 10-11 TGGTATC 2315 0.0 19.915648 2 GTAAGGT 1800 0.0 19.804434 4 CAGAGTA 14600 0.0 19.798508 10-11 GTACTTT 8575 0.0 19.72138 14-15 >>END_MODULE