FastQCFastQC Report
Thu 26 May 2016
SRR938430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3273759
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79090.24158772835752418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63700.19457754831678203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47420.14484878086627634No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36030.11005697120649383No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35730.10914059342792184No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33490.10229830601458445No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.050.7380331
GTATCAA87000.039.74371
ATCAACG113950.029.9550953
TATCAAC115950.029.5330452
TCAACGC115300.029.5214484
CAACGCA118400.028.7886545
AACGCAG123900.027.4854986
TAAGGTG19450.023.7086095
ACGCAGA143450.023.6072147
CGCAGAG145550.023.2339768
GTAAGGT17800.022.4343364
GTACATG112300.022.404181
TACATGG113050.021.8345012
ACATGGG114150.020.9901333
GCAGAGT162000.020.5229039
GAGTACT89300.020.50353212-13
GTACTTT89550.020.28717614-15
TGGTATC25950.019.793972
AGTACTT92500.019.717212-13
AGAGTAC146850.019.69695510-11
Produced by FastQC (version 0.11.5)