##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938430_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3273759 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.797569094120856 31.0 31.0 34.0 30.0 34.0 2 32.03104382454542 31.0 31.0 34.0 30.0 34.0 3 32.09745769312891 33.0 31.0 34.0 30.0 34.0 4 35.7596701528732 37.0 35.0 37.0 35.0 37.0 5 35.62656353140228 37.0 35.0 37.0 33.0 37.0 6 35.65649334602822 37.0 35.0 37.0 33.0 37.0 7 35.59578759462746 37.0 35.0 37.0 33.0 37.0 8 35.58459190184739 37.0 35.0 37.0 33.0 37.0 9 37.16993278979913 39.0 37.0 39.0 33.0 39.0 10-11 37.078543655779185 39.0 37.0 39.0 33.0 39.0 12-13 36.96756694674227 39.0 37.0 39.0 33.0 39.0 14-15 38.03428047085934 40.0 37.0 41.0 33.0 41.0 16-17 37.953370269466994 40.0 37.0 41.0 33.0 41.0 18-19 38.05398732771716 40.0 37.0 41.0 33.0 41.0 20-21 38.10523743500973 40.0 37.0 41.0 33.0 41.0 22-23 38.01389671628242 40.0 37.0 41.0 33.0 41.0 24-25 37.91008928879615 40.0 37.0 41.0 32.5 41.0 26-27 37.717227199680856 40.0 37.0 41.0 32.0 41.0 28-29 37.599361162504636 39.5 37.0 41.0 32.0 41.0 30-31 37.37614619768895 39.0 36.0 41.0 31.5 41.0 32-33 37.175779432756045 39.0 36.0 41.0 31.0 41.0 34-35 37.02833333180604 39.0 36.0 41.0 31.0 41.0 36-37 37.03022611010768 39.0 36.0 41.0 31.0 41.0 38-39 36.91085492243015 39.0 36.0 41.0 30.0 41.0 40-41 36.743160843544075 39.0 35.0 41.0 30.0 41.0 42-43 36.53286848543219 39.0 35.0 40.5 30.0 41.0 44-45 36.28516576815825 39.0 35.0 40.0 30.0 41.0 46-47 35.93813777984268 38.0 35.0 40.0 29.0 41.0 48-49 35.59219264460212 38.0 34.5 40.0 28.0 41.0 50-51 35.23702844344987 38.0 34.0 40.0 27.0 41.0 52-53 34.744249194885754 38.0 33.5 40.0 26.0 41.0 54-55 34.42571887545785 38.0 33.0 40.0 25.0 41.0 56-57 34.07951226709113 37.0 33.0 40.0 24.0 41.0 58-59 33.62456704968203 37.0 32.0 39.5 23.0 41.0 60-61 33.25215386960372 36.0 32.0 39.0 22.5 40.5 62-63 32.783830453005244 36.0 31.0 39.0 21.5 40.0 64-65 32.27230272601007 35.0 31.0 38.5 20.0 40.0 66-67 31.771509753772346 35.0 30.5 38.0 18.5 40.0 68-69 31.22176113146997 34.5 29.5 37.5 17.5 39.5 70-71 30.63119597380259 34.0 29.0 37.0 13.0 39.0 72-73 30.17194042078235 34.0 29.0 36.0 9.5 39.0 74-75 29.646262446319355 34.0 28.0 36.0 7.5 38.5 76-77 28.380753286970727 31.5 26.5 34.5 7.0 36.5 78-79 29.06957445554178 33.0 28.0 35.0 6.5 37.0 80-81 28.786895736674566 33.0 28.0 35.0 2.0 37.0 82-83 28.302359611687972 33.0 27.0 35.0 2.0 36.0 84-85 27.77696724163263 33.0 26.0 35.0 2.0 36.0 86-87 27.189549688905018 32.5 25.5 35.0 2.0 35.0 88-89 26.53490895328581 32.0 25.0 34.0 2.0 35.0 90-91 25.97588536602725 32.0 23.5 34.0 2.0 35.0 92-93 25.216231707954066 31.0 20.5 34.0 2.0 35.0 94-95 24.433292126879223 31.0 17.5 34.0 2.0 35.0 96-97 23.417633216128614 31.0 4.5 34.0 2.0 35.0 98-99 22.141113930500076 30.0 2.0 34.0 2.0 35.0 100-101 18.432684415682402 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.357691887419886 1101 2 0.4383272376214862 1101 3 0.43107405366246354 1101 4 0.2718372911147853 1101 5 0.14230727715029445 1101 6 0.1795807249375514 1101 7 0.30898866036617534 1101 8 0.31298362253419043 1101 9 0.335084919192461 1101 10-11 0.3416328415030563 1101 12-13 0.4588605055370394 1101 14-15 0.5442135281615208 1101 16-17 0.4938257920137019 1101 18-19 0.536527592601793 1101 20-21 0.5408292374403132 1101 22-23 0.5224909867082843 1101 24-25 0.5965553632923104 1101 26-27 0.6519295252995789 1101 28-29 0.5661859210566504 1101 30-31 0.5994820986230636 1101 32-33 0.5371942061143855 1101 34-35 0.3763188669299353 1101 36-37 0.5858255744760967 1101 38-39 0.5813408697483666 1101 40-41 0.6211957634526826 1101 42-43 0.7202373739413375 1101 44-45 0.6778204549409637 1101 46-47 0.737634733390216 1101 48-49 0.8061379331008425 1101 50-51 0.8767570502042759 1101 52-53 0.9325193088853609 1101 54-55 0.777430163343368 1101 56-57 0.876507450878762 1101 58-59 0.8488099265795057 1101 60-61 0.8619337701397214 1101 62-63 0.8669302884355972 1101 64-65 0.954143922331312 1101 66-67 0.8201570119139596 1101 68-69 0.8356108088394905 1101 70-71 0.8051384424588974 1101 72-73 0.6853694874239693 1101 74-75 0.795504278668183 1101 76-77 0.7919436754263955 1101 78-79 0.851010792163585 1101 80-81 0.920981454204334 1101 82-83 0.8646680020163302 1101 84-85 0.7836909556185567 1101 86-87 0.8203194054663605 1101 88-89 0.8759610480168014 1101 90-91 0.8401495235228573 1101 92-93 0.8954308879223696 1101 94-95 1.134437888951659 1101 96-97 1.24475903391588 1101 98-99 1.278561193695868 1101 100-101 1.167575287790143 1102 1 0.294678848612687 1102 2 0.2843223336979577 1102 3 0.29656353421128756 1102 4 0.2549016090254028 1102 5 0.17976500707374754 1102 6 0.24011771843994723 1102 7 0.21847727668272654 1102 8 0.20909031557712154 1102 9 0.25532371291448896 1102 10-11 0.41305574246916876 1102 12-13 0.3377428609268023 1102 14-15 0.3750114662935857 1102 16-17 0.4652702987868196 1102 18-19 0.4281549955044923 1102 20-21 0.42109738730929536 1102 22-23 0.4025026915055747 1102 24-25 0.49460870981377525 1102 26-27 0.5083050223322445 1102 28-29 0.40404652990432055 1102 30-31 0.3056776628914264 1102 32-33 0.2652671366150443 1102 34-35 0.32183685128654105 1102 36-37 0.5394399385716042 1102 38-39 0.5036595197223974 1102 40-41 0.5412157240468574 1102 42-43 0.47733066940173785 1102 44-45 0.49769363480039175 1102 46-47 0.3519597303178301 1102 48-49 0.48873827126145386 1102 50-51 0.5580582153321032 1102 52-53 0.3869277359429617 1102 54-55 0.42709562021698133 1102 56-57 0.4217046187340827 1102 58-59 0.47038337443022726 1102 60-61 0.6062979746694097 1102 62-63 0.620997135613166 1102 64-65 0.47664602344648443 1102 66-67 0.44446582430815695 1102 68-69 0.6537774768276101 1102 70-71 0.4898223534667636 1102 72-73 0.3610498721262658 1102 74-75 0.4493897559979416 1102 76-77 0.5079532315360815 1102 78-79 0.5718781860275364 1102 80-81 0.4618052796611316 1102 82-83 0.4956948328606998 1102 84-85 0.23090707711851266 1102 86-87 0.2167917123718972 1102 88-89 0.14289935650015195 1102 90-91 0.16855749269592835 1102 92-93 0.12896567723602104 1102 94-95 0.2036023884303475 1102 96-97 -0.014016342996434616 1102 98-99 -0.14946679077966607 1102 100-101 -0.3591716606373865 1103 1 0.2979562779281473 1103 2 0.3216731517623188 1103 3 0.3016223833183247 1103 4 0.21967048896249963 1103 5 0.2051029261264148 1103 6 0.1803907353892953 1103 7 0.3080683259102486 1103 8 0.22610153373304342 1103 9 0.3394911050787286 1103 10-11 0.374510846380538 1103 12-13 0.38869879377302397 1103 14-15 0.6034773573722205 1103 16-17 0.5517996045790241 1103 18-19 0.5007134208060222 1103 20-21 0.4096327058721698 1103 22-23 0.47165242750107694 1103 24-25 0.491034808188374 1103 26-27 0.5061160309319064 1103 28-29 0.5469595439421013 1103 30-31 0.5675070086219094 1103 32-33 0.564041165429991 1103 34-35 0.5404258231487304 1103 36-37 0.546621358937827 1103 38-39 0.5118846509584429 1103 40-41 0.5978254619004062 1103 42-43 0.5296144304194783 1103 44-45 0.6104453920369295 1103 46-47 0.7149433115654205 1103 48-49 0.8264670663799834 1103 50-51 0.6717566631025065 1103 52-53 0.7678670230494049 1103 54-55 0.7656888828107071 1103 56-57 0.8421695884547304 1103 58-59 0.8310056645890285 1103 60-61 0.8238144073092357 1103 62-63 0.7463751844987669 1103 64-65 0.6953765511394252 1103 66-67 0.7717044512357525 1103 68-69 0.6964733683563864 1103 70-71 0.3325041181450388 1103 72-73 0.42226802805029706 1103 74-75 0.6006585387231524 1103 76-77 0.33706958027677913 1103 78-79 0.5505312698471663 1103 80-81 0.5443764334943921 1103 82-83 0.5603547205546349 1103 84-85 0.5139265070450243 1103 86-87 0.7448210119387007 1103 88-89 0.7783402722648525 1103 90-91 0.6364865730331708 1103 92-93 0.7252744988076749 1103 94-95 0.6896797307818758 1103 96-97 0.6425472311829417 1103 98-99 0.5325435031450212 1103 100-101 0.1229748735912537 1104 1 0.19491402388175771 1104 2 0.18917240588329776 1104 3 0.2171692991085834 1104 4 0.1675960129425107 1104 5 0.15165043861988892 1104 6 0.144596933643939 1104 7 0.13975046956655035 1104 8 0.1720952310553372 1104 9 0.20744907479603825 1104 10-11 0.23287821044408474 1104 12-13 0.2764701072659719 1104 14-15 0.3626451428574171 1104 16-17 0.3391387744156731 1104 18-19 0.3401764811476298 1104 20-21 0.3797108366993456 1104 22-23 0.30798419448846914 1104 24-25 0.28129409438215447 1104 26-27 0.3302909022677767 1104 28-29 0.3081220896271901 1104 30-31 0.2618933366595044 1104 32-33 0.34499561086894204 1104 34-35 0.2540782062321867 1104 36-37 0.3277636726545481 1104 38-39 0.2933430309092131 1104 40-41 0.3464022314983666 1104 42-43 0.3334706249447308 1104 44-45 0.2082445827581907 1104 46-47 0.19165603626147742 1104 48-49 0.1742009926871333 1104 50-51 0.2853708198862748 1104 52-53 0.35952996032098383 1104 54-55 0.19520378174410524 1104 56-57 0.1719227024996357 1104 58-59 0.2962146039490605 1104 60-61 0.10402623695046742 1104 62-63 0.1228869880342387 1104 64-65 0.14571732461596554 1104 66-67 -0.01552143822497598 1104 68-69 0.03847241736063722 1104 70-71 -0.11670664861859592 1104 72-73 -0.2661992838017184 1104 74-75 -0.23281464692613696 1104 76-77 -0.16319101120528146 1104 78-79 -0.1401149410874183 1104 80-81 -0.18124917253230777 1104 82-83 -0.18658624312105943 1104 84-85 -0.007330578805650134 1104 86-87 0.16222470031044978 1104 88-89 0.08891450587416116 1104 90-91 -0.08421949469264334 1104 92-93 -0.12902689225669306 1104 94-95 0.09825967036567462 1104 96-97 -0.0738327382360211 1104 98-99 -0.06653953183670325 1104 100-101 -0.3578299298696237 1105 1 0.21096013332993024 1105 2 0.28438798670909904 1105 3 0.23072303498358337 1105 4 0.17288886057151132 1105 5 0.0831978871683603 1105 6 0.15241366308838877 1105 7 0.1678678162861118 1105 8 0.23763613448257814 1105 9 0.25072689872225595 1105 10-11 0.2550309215851172 1105 12-13 0.22807129885040212 1105 14-15 0.39562550033274135 1105 16-17 0.23005142139446377 1105 18-19 0.24627073017732215 1105 20-21 0.22335709223118982 1105 22-23 0.2829523281979931 1105 24-25 0.29822395631876475 1105 26-27 0.2177991373765451 1105 28-29 0.29288991326696845 1105 30-31 0.34627545286762285 1105 32-33 0.44422786599977115 1105 34-35 0.5269805808079084 1105 36-37 0.5596561006510541 1105 38-39 0.4627934627153536 1105 40-41 0.4152395952610277 1105 42-43 0.3861667279480372 1105 44-45 0.4275699662278285 1105 46-47 0.396505620209453 1105 48-49 0.32917258156186335 1105 50-51 0.17613138588888688 1105 52-53 0.2121712819485282 1105 54-55 0.3064113172266829 1105 56-57 0.29241236922835867 1105 58-59 0.3055330770882918 1105 60-61 0.28305965000919997 1105 62-63 0.31526494730267274 1105 64-65 0.246052189202139 1105 66-67 0.2914717803419755 1105 68-69 0.13813325619597805 1105 70-71 0.1800396778067963 1105 72-73 0.3278905039473017 1105 74-75 0.18874665808797175 1105 76-77 0.11715634926333607 1105 78-79 0.3212458390280162 1105 80-81 0.31659985286046677 1105 82-83 0.3030316120269596 1105 84-85 0.2818842035726483 1105 86-87 0.39336585365533594 1105 88-89 0.22678284796774406 1105 90-91 0.27543071011092835 1105 92-93 0.4734610952259395 1105 94-95 0.5674054926591907 1105 96-97 0.4479266569489475 1105 98-99 0.18959105908605522 1105 100-101 -0.13837000978598724 1106 1 0.17258498898853603 1106 2 0.19647635033422972 1106 3 0.20812798740360705 1106 4 0.13188589507578286 1106 5 0.058691808674737445 1106 6 0.029018978223234626 1106 7 0.10674924421936538 1106 8 0.11897205448864412 1106 9 0.11050660776371757 1106 10-11 0.10545960850686953 1106 12-13 0.1250281362075043 1106 14-15 0.26060070943461255 1106 16-17 0.10212745454170857 1106 18-19 0.32856850472098387 1106 20-21 0.17964956934025622 1106 22-23 0.297233961089006 1106 24-25 0.24051847879106703 1106 26-27 0.09737574297264473 1106 28-29 0.2786393611874658 1106 30-31 0.3629151886842408 1106 32-33 0.2845035756256209 1106 34-35 0.17713953194114396 1106 36-37 -0.18672605776907147 1106 38-39 0.11387561930926182 1106 40-41 0.1972140240062501 1106 42-43 0.34845341173431876 1106 44-45 0.248388561052046 1106 46-47 0.1333258635458634 1106 48-49 0.2528121200065456 1106 50-51 0.26650630827464994 1106 52-53 0.22119920410672478 1106 54-55 -0.1466060852181954 1106 56-57 0.12906180857969218 1106 58-59 0.08783921008747342 1106 60-61 -0.2393430301752204 1106 62-63 0.005494351787298513 1106 64-65 -0.41740118396146286 1106 66-67 -0.10731671772083473 1106 68-69 -0.7166244434165847 1106 70-71 -0.5701106339144246 1106 72-73 -0.2959372929042985 1106 74-75 -0.06042434467383373 1106 76-77 0.1415178229643992 1106 78-79 -0.03462567746272427 1106 80-81 -0.8084299987664103 1106 82-83 -0.4237514607660593 1106 84-85 -0.15005435054097305 1106 86-87 -0.0704235083486715 1106 88-89 0.09650947028074697 1106 90-91 0.07743224413335525 1106 92-93 -0.14856023920499695 1106 94-95 -0.08485606659312595 1106 96-97 -0.2113327382360204 1106 98-99 -0.2030257029185094 1106 100-101 -0.7332434845434506 1107 1 0.14601179536004594 1107 2 0.1107380546525576 1107 3 0.06067704560722831 1107 4 0.0867682462356143 1107 5 0.007324538370092171 1107 6 0.10066818178449921 1107 7 0.042216508467440406 1107 8 0.04667176982993482 1107 9 0.05640252563664916 1107 10-11 0.06615815795875335 1107 12-13 0.11370803142887098 1107 14-15 0.18735219329385444 1107 16-17 0.23546848476399163 1107 18-19 0.21300918063401042 1107 20-21 0.1635277414679308 1107 22-23 0.17460548613291849 1107 24-25 0.25732924131302326 1107 26-27 0.20650374854468367 1107 28-29 0.3294482915974939 1107 30-31 0.15657358308796887 1107 32-33 0.33076440174153987 1107 34-35 0.2266065539264872 1107 36-37 0.13376861245077976 1107 38-39 0.15891566836746307 1107 40-41 0.16886549878064017 1107 42-43 0.347783403860781 1107 44-45 0.31674450557387956 1107 46-47 0.1918493478896437 1107 48-49 0.2353237789006144 1107 50-51 0.21332206746549076 1107 52-53 0.4404450856402491 1107 54-55 0.30132256840666116 1107 56-57 0.26051205387070553 1107 58-59 0.15578633624578941 1107 60-61 0.09017024885088176 1107 62-63 0.1627588269856588 1107 64-65 0.17795002129563642 1107 66-67 0.011526402017523196 1107 68-69 0.15536052115249177 1107 70-71 0.10187319504808201 1107 72-73 0.12187696728587838 1107 74-75 0.06785755829994145 1107 76-77 0.16517440039762832 1107 78-79 0.1077282835329747 1107 80-81 0.347549348335523 1107 82-83 0.42436305379998984 1107 84-85 0.48144699704548444 1107 86-87 0.45628028003483223 1107 88-89 0.39015840129642854 1107 90-91 0.36227265355737615 1107 92-93 0.19781868999372065 1107 94-95 0.04584467836478012 1107 96-97 0.07006110526225129 1107 98-99 -0.19934444496926318 1107 100-101 -0.5874448502996117 1108 1 0.15960758281122978 1108 2 0.18659599543379102 1108 3 0.1722021403268883 1108 4 0.1533116815250608 1108 5 0.05110240411973166 1108 6 0.10291336384435823 1108 7 0.08113972947575121 1108 8 0.17317611387844067 1108 9 0.2299382046902494 1108 10-11 0.15189294725253433 1108 12-13 0.19867709397318123 1108 14-15 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0.02986164708968886 2316 70-71 -0.1052392899768364 2316 72-73 -0.25558520807677354 2316 74-75 -0.016768706839279446 2316 76-77 -0.05900870862030061 2316 78-79 -0.01791755647423443 2316 80-81 -0.18395229339602182 2316 82-83 0.03318182061267194 2316 84-85 0.04243837031754438 2316 86-87 0.335058313271837 2316 88-89 0.34768625604766257 2316 90-91 0.44238829208410735 2316 92-93 0.30812606190338343 2316 94-95 0.23445139564095285 2316 96-97 0.5539301687748335 2316 98-99 0.5979017516381724 2316 100-101 0.48687848186688143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 19.0 4 40.0 5 91.0 6 224.0 7 589.0 8 1359.0 9 2391.0 10 3788.0 11 6015.0 12 8528.0 13 12138.0 14 15744.0 15 20380.0 16 25140.0 17 30569.0 18 30243.0 19 27557.0 20 27707.0 21 30081.0 22 32727.0 23 36747.0 24 42734.0 25 50008.0 26 59637.0 27 71883.0 28 85681.0 29 102437.0 30 121883.0 31 145157.0 32 175096.0 33 214712.0 34 273903.0 35 355502.0 36 471029.0 37 527996.0 38 254244.0 39 9772.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49542877285843 18.986522440097527 15.03865000803363 23.479398779010417 2 16.327857169488073 20.199759064274588 38.907483004007645 24.564900762229687 3 19.75424302979911 23.39651931349088 29.23928788088909 27.609949775820912 4 13.68420827556335 16.73223349672349 35.57344935897847 34.01010886873468 5 14.302472579967136 36.939289945351014 33.796186035846674 14.962051438835175 6 33.64236646619376 35.51678666633677 16.658617815178207 14.182229052291264 7 30.03623663195733 31.627557190373512 19.68969004743477 18.64651613023439 8 26.96667653300075 35.146417314163934 18.952403032721712 18.934503120113607 9 26.379400560639926 16.73476880857754 19.309698728586923 37.576131902195606 10-11 25.535065348426688 26.298194216495475 26.830899892142334 21.335840542935504 12-13 26.37423829915397 23.220218714939005 27.26213200177533 23.143410984131695 14-15 22.8018922590209 24.993547173142556 25.29474527599619 26.90981529184036 16-17 22.92493124875716 28.17018601552527 26.03537706960103 22.869505666116535 18-19 22.94391554173658 26.645027932721987 28.131117776232156 22.27993874930928 20-21 23.265457231274507 25.916339596164534 28.383656219043612 22.434546953517348 22-23 23.084289344450827 25.741479443050025 28.29855526934023 22.875675943158917 24-25 23.051057655593738 25.988560519282167 28.279725085910652 22.68065673921344 26-27 22.55538241259191 26.39588240846784 28.12837276196766 22.920362416972587 28-29 22.714973826723348 26.638582742346028 28.096921612128444 22.54952181880218 30-31 22.741869514524435 26.165670716750988 28.000549215748627 23.091910552975953 32-33 22.495638805422146 26.21084814123459 27.982007838695516 23.31150521464775 34-35 23.289849017956087 26.24345768661202 27.709806539220278 22.756886756211617 36-37 23.33678451650268 25.838451943508932 27.80170119555318 23.023062344435207 38-39 22.762676313085638 26.194753171226125 27.815392491879393 23.227178023808843 40-41 23.182640260669185 26.00264634270581 27.92662882729567 22.888084569329337 42-43 23.019595151544404 26.024961943414322 27.654304620994285 23.301138284046992 44-45 22.950605099520153 26.257934686090213 27.818770410405897 22.97268980398374 46-47 23.264342304977244 26.553054149679312 27.176664500960516 23.005939044382924 48-49 23.13571341079169 26.455398824409492 27.22842457248686 23.180463192311958 50-51 23.29245982981643 26.62434834085221 27.164415584653607 22.918776244677755 52-53 23.60835052305316 26.387556322869216 27.040444944175796 22.96364820990183 54-55 23.01137621920245 26.551679583011456 27.307034512925355 23.129909684860735 56-57 23.09131490741988 26.688158780166777 27.18170457874266 23.038821733670684 58-59 23.087954855565116 26.276399698328433 27.172846260216467 23.46279918588998 60-61 22.77681405381398 26.530908353363824 27.481329566409745 23.21094802641245 62-63 22.858952048699983 26.638872928642577 27.41206667931268 23.090108343344763 64-65 22.581961592163626 26.53547496929371 27.328691574425605 23.55387186411706 66-67 22.550292355146592 26.62499793814937 27.28753927443629 23.537170432267747 68-69 22.990844991045375 26.284705474830368 27.37984871676838 23.344600817355875 70-71 22.79712574879565 26.45598008648779 27.074092548713907 23.672801616002655 72-73 22.83291164682556 26.49045027443987 27.081804127915344 23.59483395081923 74-75 22.745962668602058 26.839910940298296 26.871327425140336 23.542798965959314 76-77 22.828390849784604 26.502057726301782 27.09446541422261 23.575086009691002 78-79 22.960700528047422 26.63722344864115 27.099490219041783 23.302585804269647 80-81 22.89827616147605 26.87463449360153 26.86516511916215 23.361924225760273 82-83 22.904367096679106 26.83477701743494 26.8602829941573 23.400572891728654 84-85 22.83543978216018 26.78885739788453 26.89386418246834 23.48183863748695 86-87 22.936707422365593 26.764112156498467 26.71838399043771 23.580796430698232 88-89 22.79909113554754 26.939626370998077 26.515480462728508 23.745802030725873 90-91 22.70512829324432 26.88883441354878 26.730937385687696 23.6750999075192 92-93 22.760090379893068 26.536203121997886 26.90208468181775 23.80162181629129 94-95 22.549642505537214 26.495781452319157 26.904043275050654 24.050532767092974 96-97 22.70069707340966 26.29732767398695 26.892927502135542 24.109047750467848 98-99 22.446254767651403 26.530661804157337 26.573395600251757 24.449687827939503 100-101 22.526704012115736 26.269664321655934 26.959238600031338 24.24439306619699 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 446.0 1 320.0 2 279.0 3 378.5 4 317.0 5 286.0 6 543.0 7 1350.5 8 2123.0 9 2318.5 10 2040.0 11 1664.5 12 1667.0 13 1827.5 14 2054.5 15 2306.5 16 2517.0 17 2748.5 18 3039.0 19 3299.5 20 3716.5 21 4306.5 22 5132.0 23 6256.0 24 7514.0 25 9458.5 26 12179.5 27 15568.5 28 19089.5 29 23310.0 30 28847.0 31 35827.0 32 45762.0 33 50906.5 34 52163.5 35 57024.5 36 64613.5 37 72066.0 38 77769.0 39 82613.5 40 88724.0 41 98057.5 42 105991.5 43 112041.5 44 117376.0 45 123845.0 46 127889.0 47 130746.5 48 132883.0 49 131338.0 50 130158.0 51 126639.0 52 122426.0 53 121651.5 54 123737.0 55 117327.0 56 104377.0 57 94810.5 58 87706.5 59 77294.5 60 62767.0 61 52448.5 62 44305.0 63 33854.5 64 24481.0 65 18339.5 66 13733.5 67 10731.0 68 8192.0 69 5997.5 70 4596.0 71 3417.5 72 2650.0 73 2265.0 74 1818.0 75 1347.5 76 934.0 77 561.0 78 354.5 79 212.0 80 119.0 81 80.5 82 50.5 83 26.5 84 16.0 85 6.5 86 4.0 87 2.5 88 1.5 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.414644450003803E-4 2 0.046338169669789375 3 0.0011301992602387654 4 0.0 5 0.0880944504467189 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.749133335715916E-4 26-27 0.008919410378100526 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010889622602030264 36-37 7.636481488099766E-5 38-39 0.004383340374169265 40-41 0.005635723338217627 42-43 4.7346185226218547E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.054592595239906E-5 68-69 9.774696304767699E-4 70-71 6.109185190479812E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014509314827389553 82-83 5.040077782145845E-4 84-85 0.004917894078336249 86-87 0.0020007581498821384 88-89 1.0691074083339671E-4 90-91 0.0032531411139304998 92-93 6.720103709527794E-4 94-95 6.109185190479812E-5 96-97 1.527296297619953E-5 98-99 1.0691074083339671E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3273759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.4345390044297 #Duplication Level Percentage of deduplicated Percentage of total 1 74.61115388600994 37.629791508294744 2 13.815562547496157 13.93563056339666 3 4.677243014955726 7.076837858129428 4 2.2032924616735734 4.44488158385767 5 1.1906904093034547 3.002596094510772 6 0.7536529254062887 2.280608271732361 7 0.4869880941231022 1.7192714019421134 8 0.3532211709789775 1.4251637539943656 9 0.267550785893471 1.2144420492129073 >10 1.4673389264914956 13.492732622367273 >50 0.10888881081179227 3.775527975932557 >100 0.05750150772924385 5.393170383208769 >500 0.003882363020007161 1.3591327689734791 >1k 0.002911772262463949 2.8133534310713992 >5k 1.2132384426933121E-4 0.43685973337540246 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7909 0.24158772835752418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6370 0.19457754831678203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4742 0.14484878086627634 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3603 0.11005697120649383 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3573 0.10914059342792184 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3349 0.10229830601458445 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 3.054592595239906E-5 0.0 0.0 0.0 0.0 16-17 4.581888892859859E-5 0.0 0.0 0.0 0.0 18-19 6.109185190479812E-5 0.0 0.0 3.054592595239906E-5 0.0 20-21 6.109185190479812E-5 0.0 0.0 6.109185190479812E-5 0.0 22-23 6.109185190479812E-5 0.0 0.0 1.8327555571439436E-4 0.0 24-25 6.109185190479812E-5 0.0 0.0 5.650996301193827E-4 0.0 26-27 6.109185190479812E-5 0.0 0.0 0.0011454722232149648 0.0 28-29 6.109185190479812E-5 0.0 0.0 0.0028102251876207136 0.0 30-31 6.109185190479812E-5 0.0 0.0 0.009285961489529315 0.0 32-33 6.109185190479812E-5 0.0 0.0 0.0228178066864421 0.0 34-35 6.109185190479812E-5 0.0 0.0 0.045727251150741395 0.0 36-37 1.2218370380959623E-4 0.0 0.0 0.08371111007254962 0.0 38-39 1.2218370380959623E-4 0.0 0.0 0.13902978197234434 0.0 40-41 1.374566667857958E-4 0.0 0.0 0.20070200647023803 0.0 42-43 1.527296297619953E-4 0.0 0.0 0.2620993176345601 0.0 44-45 1.527296297619953E-4 0.0 0.0 0.34316820511222723 1.527296297619953E-5 46-47 1.527296297619953E-4 0.0 0.0 0.4411595355675234 3.054592595239906E-5 48-49 1.8327555571439436E-4 0.0 0.0 0.547367720104015 3.054592595239906E-5 50-51 1.8327555571439436E-4 0.0 0.0 0.6594712683493196 3.054592595239906E-5 52-53 1.8327555571439436E-4 0.0 0.0 0.7712235384461714 3.054592595239906E-5 54-55 1.8327555571439436E-4 0.0 0.0 0.8947207170717209 3.054592595239906E-5 56-57 1.8327555571439436E-4 0.0 0.0 1.0423186312737132 3.054592595239906E-5 58-59 1.8327555571439436E-4 0.0 0.0 1.2004090710403545 3.054592595239906E-5 60-61 1.8327555571439436E-4 0.0 0.0 1.3739862952648623 3.054592595239906E-5 62-63 2.1382148166679344E-4 0.0 0.0 1.5425998065221052 3.054592595239906E-5 64-65 2.1382148166679344E-4 0.0 0.0 1.7231109559378073 3.054592595239906E-5 66-67 2.4436740761919247E-4 0.0 0.0 1.940017576125793 3.054592595239906E-5 68-69 2.4436740761919247E-4 0.0 0.0 2.1763514052195045 6.109185190479812E-5 70-71 2.5964037059539205E-4 0.0 0.0 2.4300964121060833 6.109185190479812E-5 72-73 2.749133335715916E-4 0.0 0.0 2.6946394038168355 6.109185190479812E-5 74-75 2.749133335715916E-4 0.0 0.0 2.966101047755806 9.163777785719718E-5 76-77 2.749133335715916E-4 0.0 0.0 3.2614343328265765 9.163777785719718E-5 78-79 2.749133335715916E-4 0.0 0.0 3.590841598297248 9.163777785719718E-5 80-81 3.2073222250019015E-4 0.0 0.0 3.944089958973767 9.163777785719718E-5 82-83 3.360051854763897E-4 0.0 0.0 4.30966360077208 9.163777785719718E-5 84-85 3.512781484525892E-4 3.054592595239906E-5 0.0 4.675649612570748 9.163777785719718E-5 86-87 3.6655111142878873E-4 3.054592595239906E-5 0.0 5.059520264014548 9.163777785719718E-5 88-89 3.6655111142878873E-4 3.054592595239906E-5 0.0 5.476945615117057 9.163777785719718E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5240 0.0 50.738033 1 GTATCAA 8700 0.0 39.7437 1 ATCAACG 11395 0.0 29.955095 3 TATCAAC 11595 0.0 29.533045 2 TCAACGC 11530 0.0 29.521448 4 CAACGCA 11840 0.0 28.788654 5 AACGCAG 12390 0.0 27.485498 6 TAAGGTG 1945 0.0 23.708609 5 ACGCAGA 14345 0.0 23.607214 7 CGCAGAG 14555 0.0 23.233976 8 GTAAGGT 1780 0.0 22.434336 4 GTACATG 11230 0.0 22.40418 1 TACATGG 11305 0.0 21.834501 2 ACATGGG 11415 0.0 20.990133 3 GCAGAGT 16200 0.0 20.522903 9 GAGTACT 8930 0.0 20.503532 12-13 GTACTTT 8955 0.0 20.287176 14-15 TGGTATC 2595 0.0 19.79397 2 AGTACTT 9250 0.0 19.7172 12-13 AGAGTAC 14685 0.0 19.696955 10-11 >>END_MODULE