FastQCFastQC Report
Thu 26 May 2016
SRR938429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270778
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76890.23508168392963386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59930.1832285774210295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42980.1314060446780552No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36480.11153309701850751No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35540.10865916304928062No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA33220.10156604942310361No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32780.10022080373538039No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.049.2610661
GTATCAA85950.043.626951
ATCAACG109600.033.6474953
TATCAAC111400.033.2744562
TCAACGC111350.033.1186874
CAACGCA113650.032.4066285
AACGCAG117650.031.3413896
ACGCAGA137050.026.7249837
CGCAGAG138600.026.2890138
GTACATG115150.026.2745821
TACATGG115050.025.4032962
TGGTATC23950.025.1999552
GTGGTAT26250.024.1406151
GCAGAGT152250.023.4640359
ACATGGG121100.023.3885733
GAGTACT85750.021.18798412-13
TAAGGTG18600.020.9508915
AGAGTAC139100.020.55740510-11
GTGTAAG13700.020.5190581
GGACCGA10550.020.2678876
Produced by FastQC (version 0.11.5)