##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938429_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3270778 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99191935374397 31.0 31.0 34.0 28.0 34.0 2 31.131340922557264 31.0 31.0 34.0 27.0 34.0 3 31.189154996150762 31.0 31.0 34.0 27.0 34.0 4 34.85354035033867 37.0 35.0 37.0 32.0 37.0 5 34.85293590699216 37.0 35.0 37.0 32.0 37.0 6 34.85280077094807 37.0 35.0 37.0 32.0 37.0 7 34.76182516820157 37.0 35.0 37.0 32.0 37.0 8 34.711381206550854 37.0 35.0 37.0 31.0 37.0 9 36.18343831345325 39.0 35.0 39.0 31.0 39.0 10-11 36.03071914388565 39.0 35.0 39.0 30.0 39.0 12-13 35.84578669050605 38.0 35.0 39.0 30.0 39.0 14-15 36.810230776897725 39.0 36.0 41.0 30.5 41.0 16-17 36.620827674638875 39.0 36.0 40.5 30.0 41.0 18-19 36.457581346089526 39.0 36.0 40.0 29.5 41.0 20-21 36.4234845654459 39.0 35.5 40.5 29.5 41.0 22-23 36.526017663075876 39.0 36.0 41.0 30.0 41.0 24-25 36.42872231010482 39.0 36.0 41.0 29.5 41.0 26-27 36.218062185816336 39.0 35.0 41.0 28.5 41.0 28-29 36.015912574928656 39.0 35.0 40.0 27.5 41.0 30-31 35.74344208014118 38.0 35.0 40.0 27.0 41.0 32-33 35.43835656226133 38.0 34.0 40.0 26.5 41.0 34-35 35.15935535826644 38.0 34.0 40.0 25.5 41.0 36-37 34.854036868292496 38.0 34.0 40.0 25.0 41.0 38-39 34.51052089135979 38.0 33.0 40.0 24.0 41.0 40-41 34.3865239401757 38.0 33.0 40.0 24.0 41.0 42-43 34.137010368786875 38.0 33.0 40.0 23.5 41.0 44-45 33.81740812124822 38.0 33.0 40.0 22.5 41.0 46-47 33.413279501085064 37.5 32.0 40.0 19.5 41.0 48-49 33.00988755580477 37.0 31.0 40.0 17.5 41.0 50-51 32.212733637073505 36.5 30.5 39.5 15.0 40.5 52-53 32.218130671051355 36.5 31.0 39.0 13.5 40.0 54-55 32.24578265476899 37.0 31.0 39.0 11.0 41.0 56-57 31.8674478977173 36.0 30.5 39.0 8.0 41.0 58-59 31.386243425876046 36.0 29.5 39.0 6.5 41.0 60-61 30.855104809925955 35.0 28.5 39.0 2.0 40.0 62-63 30.22237079373776 35.0 28.0 38.5 2.0 40.0 64-65 29.67042566019461 34.5 27.5 38.0 2.0 40.0 66-67 29.07551628389331 34.0 26.0 38.0 2.0 40.0 68-69 28.410823510491998 34.0 26.0 37.0 2.0 39.5 70-71 27.72203249502106 33.5 25.0 36.0 2.0 39.0 72-73 27.039717308848232 33.0 23.0 36.0 2.0 39.0 74-75 26.293555233647773 32.0 21.0 35.0 2.0 38.0 76-77 25.507606141413447 31.5 19.0 35.0 2.0 37.0 78-79 24.66966498490573 31.0 13.0 35.0 2.0 36.5 80-81 23.861539058902807 30.5 7.0 34.5 2.0 36.0 82-83 23.09133377441086 30.0 2.0 34.0 2.0 35.5 84-85 22.31916672424726 29.5 2.0 34.0 2.0 35.0 86-87 21.561285877549622 29.0 2.0 34.0 2.0 35.0 88-89 20.758541240035246 28.0 2.0 34.0 2.0 35.0 90-91 19.919579836968452 26.5 2.0 34.0 2.0 35.0 92-93 19.035589850488172 25.5 2.0 33.0 2.0 35.0 94-95 18.125497664470043 24.5 2.0 33.0 2.0 35.0 96-97 17.05505356829476 19.0 2.0 32.5 2.0 34.5 98-99 15.815862923133274 2.0 2.0 32.0 2.0 34.0 100-101 13.175625034777658 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6640782446233295 1101 2 0.6717668586994066 1101 3 0.64568322793912 1101 4 0.510423330454806 1101 5 0.582532820344781 1101 6 0.49226881004489087 1101 7 0.6151916972846081 1101 8 0.5642123341329892 1101 9 0.6764516024319036 1101 10-11 0.704052107433462 1101 12-13 0.7757195565840291 1101 14-15 1.0057748206544588 1101 16-17 1.1112653601360378 1101 18-19 1.2278880881379521 1101 20-21 1.1983507278653107 1101 22-23 1.219626234244103 1101 24-25 1.1742800049551931 1101 26-27 1.2794214381313722 1101 28-29 1.3996757643133932 1101 30-31 1.3476425740420268 1101 32-33 1.4452209353651284 1101 34-35 1.4569581025971061 1101 36-37 1.6300100915977254 1101 38-39 1.8754322486601254 1101 40-41 1.9207013526169092 1101 42-43 2.0137800896304654 1101 44-45 2.0491085077236235 1101 46-47 2.232812439532111 1101 48-49 2.339838926633611 1101 50-51 2.3657068703403183 1101 52-53 2.4371423770526306 1101 54-55 2.6621655326376654 1101 56-57 2.7062360073719596 1101 58-59 2.973375249488683 1101 60-61 3.042213320414877 1101 62-63 3.038012457181697 1101 64-65 3.215653575169153 1101 66-67 3.34889381991972 1101 68-69 3.6101285036989275 1101 70-71 3.586239118710303 1101 72-73 3.8538160341123593 1101 74-75 3.940954475870619 1101 76-77 4.195372380646038 1101 78-79 4.619426539231171 1101 80-81 5.015018995841359 1101 82-83 5.1135011081595785 1101 84-85 5.260436208905457 1101 86-87 5.527138157290505 1101 88-89 5.886598801381158 1101 90-91 6.276652194151765 1101 92-93 6.15416893480382 1101 94-95 6.31194347845009 1101 96-97 6.376305887761248 1101 98-99 6.574347348092472 1101 100-101 6.141275609073013 1102 1 0.6336291198855228 1102 2 0.5048016828038051 1102 3 0.49607773939282396 1102 4 0.5134276527737711 1102 5 0.4770395405005132 1102 6 0.5084681869919265 1102 7 0.5781596896399321 1102 8 0.6225944081952335 1102 9 0.6198262655365809 1102 10-11 0.6965192610636493 1102 12-13 0.6863926667898497 1102 14-15 1.0281072457387026 1102 16-17 1.0993209970494107 1102 18-19 1.1408313773013887 1102 20-21 0.9719171291575677 1102 22-23 0.754478591993653 1102 24-25 0.8562653132125746 1102 26-27 0.8726533552469817 1102 28-29 0.8473356176326448 1102 30-31 0.8782612188870473 1102 32-33 0.9601761293961246 1102 34-35 0.9461962384084401 1102 36-37 1.0745085280483906 1102 38-39 1.3084008031240018 1102 40-41 1.4056608068390872 1102 42-43 1.5912247441260021 1102 44-45 1.7735980983606723 1102 46-47 1.696073024184173 1102 48-49 1.5359398162443583 1102 50-51 1.7746975008782044 1102 52-53 1.7739254297754528 1102 54-55 1.901708242533438 1102 56-57 2.0044311951310085 1102 58-59 2.0382678719617786 1102 60-61 2.150460192576592 1102 62-63 2.532607339627045 1102 64-65 2.568697836780391 1102 66-67 2.647480560666377 1102 68-69 2.7614321517834703 1102 70-71 2.84855757983458 1102 72-73 2.8980470252280846 1102 74-75 3.121960531850714 1102 76-77 3.1945453414493237 1102 78-79 3.7864960010566016 1102 80-81 4.138538653369324 1102 82-83 4.460204489390481 1102 84-85 4.533885011170462 1102 86-87 4.81631401739056 1102 88-89 5.150861714346039 1102 90-91 5.395431501456514 1102 92-93 5.182241523727914 1102 94-95 5.355932910809198 1102 96-97 5.483201184687697 1102 98-99 5.6168223416578105 1102 100-101 5.452144273887676 1103 1 0.5742109472851773 1103 2 0.5630665689379697 1103 3 0.522069854504192 1103 4 0.5007593222183502 1103 5 0.46272237814557116 1103 6 0.510113206904272 1103 7 0.4598824529913088 1103 8 0.521593978404951 1103 9 0.6897889757502469 1103 10-11 0.6711083634499886 1103 12-13 0.6394763882209489 1103 14-15 0.9424441822583915 1103 16-17 0.9846327951261244 1103 18-19 1.084477771399058 1103 20-21 1.0697049004329031 1103 22-23 0.9652453149809546 1103 24-25 1.123221111521815 1103 26-27 1.2811320406033317 1103 28-29 1.29633537843673 1103 30-31 1.308741449963648 1103 32-33 1.4025574057874906 1103 34-35 1.4539109739254172 1103 36-37 1.477177468505765 1103 38-39 1.5526207909450207 1103 40-41 1.5773612611207426 1103 42-43 1.8029985200575567 1103 44-45 1.7821579769139504 1103 46-47 2.0794394732873798 1103 48-49 1.968103996131127 1103 50-51 2.099028157313761 1103 52-53 2.297797706777672 1103 54-55 2.477135817153517 1103 56-57 2.6967213197701874 1103 58-59 2.9275681428916585 1103 60-61 3.0499297208269702 1103 62-63 3.149013320859222 1103 64-65 3.3829916036777767 1103 66-67 3.416207552353608 1103 68-69 3.4526618570578833 1103 70-71 3.8152810912688118 1103 72-73 3.962534674237137 1103 74-75 4.267586343663563 1103 76-77 4.5031415758765725 1103 78-79 4.620147674618021 1103 80-81 5.14671538961246 1103 82-83 5.186536958378394 1103 84-85 5.438000086326582 1103 86-87 5.354737268377598 1103 88-89 5.724168211993632 1103 90-91 6.127559849603795 1103 92-93 6.1874850604349625 1103 94-95 6.309848147779931 1103 96-97 6.30428787748485 1103 98-99 6.419004647068116 1103 100-101 6.1809904918761625 1104 1 0.5804746684839337 1104 2 0.4577839378734829 1104 3 0.5344351043675815 1104 4 0.43814583226808423 1104 5 0.35552258351589217 1104 6 0.45950656177317484 1104 7 0.45253193147886606 1104 8 0.4551715383874537 1104 9 0.6448391170572734 1104 10-11 0.7088682381387841 1104 12-13 0.6977496188305068 1104 14-15 0.7659363640541628 1104 16-17 0.8953471383065832 1104 18-19 1.0502538594567312 1104 20-21 1.0042931295396045 1104 22-23 1.0151505509586798 1104 24-25 1.017712155671191 1104 26-27 1.0042197800522459 1104 28-29 0.9268126520353945 1104 30-31 1.234468854516244 1104 32-33 1.3198623837466812 1104 34-35 1.3997691016148934 1104 36-37 1.4219890681774459 1104 38-39 1.4855902668433885 1104 40-41 1.627137313135627 1104 42-43 1.5917685811546676 1104 44-45 1.6712685319976757 1104 46-47 1.7608578911323178 1104 48-49 1.920225707100542 1104 50-51 1.9703525632537264 1104 52-53 2.0557286596505335 1104 54-55 2.3168835709846007 1104 56-57 2.5836483900443348 1104 58-59 2.671320719100315 1104 60-61 2.6810410532211684 1104 62-63 2.943427617781712 1104 64-65 3.0376135897952317 1104 66-67 3.255132482495423 1104 68-69 3.462642582442843 1104 70-71 3.696316499351756 1104 72-73 3.860640110969502 1104 74-75 3.822143121762199 1104 76-77 3.9490690767332772 1104 78-79 4.154353013492379 1104 80-81 4.526712866617487 1104 82-83 4.736070457159148 1104 84-85 4.876220729408946 1104 86-87 5.030192536108835 1104 88-89 5.375741134777574 1104 90-91 5.431336122104902 1104 92-93 5.500689003846684 1104 94-95 5.586365592717911 1104 96-97 5.673243573637919 1104 98-99 5.702210373310473 1104 100-101 5.48545162327207 1105 1 0.6223939733836339 1105 2 0.6815697378468784 1105 3 0.5668364725062318 1105 4 0.5196802113479109 1105 5 0.45269090987692806 1105 6 0.5183933435353651 1105 7 0.5972298097162252 1105 8 0.5469356958257734 1105 9 0.6794943255361758 1105 10-11 0.6790604690227227 1105 12-13 0.7552581927225575 1105 14-15 0.8831880461983772 1105 16-17 0.9164334751810586 1105 18-19 0.9281681104405024 1105 20-21 0.9004248310393947 1105 22-23 1.0258839674317173 1105 24-25 0.9281860749746755 1105 26-27 1.0675076610863172 1105 28-29 1.0983147139672056 1105 30-31 1.0644304161326872 1105 32-33 1.1404122800523169 1105 34-35 1.349547029707729 1105 36-37 1.4467968983995902 1105 38-39 1.4876795192751047 1105 40-41 1.5366104210565865 1105 42-43 1.6717311169260824 1105 44-45 1.7586751171789317 1105 46-47 1.8254333393454374 1105 48-49 1.9916044764381766 1105 50-51 1.8664526047173027 1105 52-53 1.9742019852508434 1105 54-55 2.215266987395644 1105 56-57 2.4641394580175096 1105 58-59 2.5145248546892276 1105 60-61 2.7773082862438123 1105 62-63 3.038333956324646 1105 64-65 3.198977311095515 1105 66-67 3.160255899958546 1105 68-69 3.1172714834242434 1105 70-71 3.311954228125405 1105 72-73 3.7314123005811446 1105 74-75 3.7536078221944926 1105 76-77 3.6860053487556037 1105 78-79 3.9053830052439444 1105 80-81 4.341902386693846 1105 82-83 4.709418330198066 1105 84-85 4.857170376401758 1105 86-87 5.206201614524588 1105 88-89 5.420939843791995 1105 90-91 5.581461548984038 1105 92-93 5.728963886996798 1105 94-95 5.862822932966683 1105 96-97 6.026764408775652 1105 98-99 6.05084573276214 1105 100-101 5.643651643499069 1106 1 0.22254794302420322 1106 2 0.3007670530824349 1106 3 0.14872256024190378 1106 4 0.18653265705053457 1106 5 0.24232651038133923 1106 6 0.14642803180387176 1106 7 0.29099851411452704 1106 8 0.2938009378199311 1106 9 0.23190770401334504 1106 10-11 0.16323120019074366 1106 12-13 0.12774618347665267 1106 14-15 0.4372071030562381 1106 16-17 0.3071397736168251 1106 18-19 0.5289808469270909 1106 20-21 0.5401069172490551 1106 22-23 0.3910508544477551 1106 24-25 0.36819442099256605 1106 26-27 0.5596688796189611 1106 28-29 0.32895286495572407 1106 30-31 0.6296871158476733 1106 32-33 0.850339110253671 1106 34-35 0.7701985280074695 1106 36-37 0.8894587376190728 1106 38-39 0.8330399686263874 1106 40-41 0.9946526410973533 1106 42-43 0.6768724464558602 1106 44-45 0.8262821615268905 1106 46-47 1.3039549780990427 1106 48-49 1.3117280487444205 1106 50-51 0.6480914881759361 1106 52-53 0.9308953477098427 1106 54-55 1.0035130180358394 1106 56-57 1.054255393261112 1106 58-59 0.388504810207543 1106 60-61 1.6928691698828437 1106 62-63 2.05388771531425 1106 64-65 2.0807439293403043 1106 66-67 2.1971562317285525 1106 68-69 1.8384843023181112 1106 70-71 2.1942306273205645 1106 72-73 2.6779195201674035 1106 74-75 2.9812291404090665 1106 76-77 3.1028698151105836 1106 78-79 3.3542582056027754 1106 80-81 3.8633975944019454 1106 82-83 3.9877297742451745 1106 84-85 4.196394589821157 1106 86-87 4.421386295615804 1106 88-89 4.239426150601862 1106 90-91 4.584582531497759 1106 92-93 5.0809927274372875 1106 94-95 5.115505995218218 1106 96-97 5.321669880822281 1106 98-99 5.47376869564763 1106 100-101 4.791836718025358 1107 1 0.5966877598988667 1107 2 0.48024203517586983 1107 3 0.5987597524580011 1107 4 0.5166353385963873 1107 5 0.5233262440454283 1107 6 0.4249267936435359 1107 7 0.5093925861148634 1107 8 0.47799066897580644 1107 9 0.5441731193896331 1107 10-11 0.5290036734688073 1107 12-13 0.5540667226301892 1107 14-15 0.8998775817454785 1107 16-17 0.939246654478481 1107 18-19 1.0378860770232237 1107 20-21 1.0482605025700664 1107 22-23 0.9998102031400791 1107 24-25 0.9066920278480097 1107 26-27 1.0120098344116784 1107 28-29 1.100558180986134 1107 30-31 1.0405917615562572 1107 32-33 1.1652676262109765 1107 34-35 1.23548599410136 1107 36-37 1.2858066602420237 1107 38-39 1.5446465258338762 1107 40-41 1.5958727440288598 1107 42-43 1.4586812724316331 1107 44-45 1.5148900090413875 1107 46-47 1.5486330865796418 1107 48-49 1.75669554972918 1107 50-51 1.8711410947486584 1107 52-53 1.9679376744341965 1107 54-55 2.115313293680586 1107 56-57 2.3338438482456034 1107 58-59 2.509959762805792 1107 60-61 2.457078665059928 1107 62-63 2.627395695479553 1107 64-65 2.8544097717159893 1107 66-67 2.991731296653324 1107 68-69 3.1948660211587487 1107 70-71 3.0932395387996436 1107 72-73 3.471099984729644 1107 74-75 3.5111211235256086 1107 76-77 3.649370169794178 1107 78-79 3.797586749486573 1107 80-81 4.1906237618625894 1107 82-83 4.342005887878383 1107 84-85 4.5124926996980825 1107 86-87 4.866866514786992 1107 88-89 5.02466553832231 1107 90-91 5.276072452536489 1107 92-93 5.503032196868563 1107 94-95 5.4854062011329425 1107 96-97 5.528634909796008 1107 98-99 5.5958594498264755 1107 100-101 5.342401970755731 1108 1 0.22567806417658076 1108 2 0.3525997831743233 1108 3 0.2520342550085637 1108 4 0.34867957113736026 1108 5 0.20753760198558524 1108 6 0.2058504610732257 1108 7 0.19196756717645513 1108 8 0.24990430573782874 1108 9 0.3013508054437679 1108 10-11 0.26035233885138354 1108 12-13 0.3697520418590514 1108 14-15 0.3782540183792875 1108 16-17 0.5907614593522297 1108 18-19 0.6938136432335753 1108 20-21 0.5553118022939216 1108 22-23 0.4888626739383213 1108 24-25 0.5162234869959832 1108 26-27 0.42000334289044616 1108 28-29 0.5762481005704316 1108 30-31 0.6377786318620338 1108 32-33 0.5972456103418153 1108 34-35 0.6936860453718694 1108 36-37 0.7546635295232065 1108 38-39 0.8198272790659544 1108 40-41 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2311 50-51 1.032468665689784 2311 52-53 1.3274743882996702 2311 54-55 1.209285400445232 2311 56-57 1.3401524997709764 2311 58-59 1.4513709731507873 2311 60-61 1.3784167063482933 2311 62-63 1.6868673912028704 2311 64-65 1.8384934456646036 2311 66-67 1.787648354724265 2311 68-69 1.6066459843027978 2311 70-71 1.9023561844721542 2311 72-73 1.9452403655196413 2311 74-75 2.153381682188133 2311 76-77 2.313394286960076 2311 78-79 2.4317922964676306 2311 80-81 2.5263486195608706 2311 82-83 2.7363575755741607 2311 84-85 2.8284030215440517 2311 86-87 3.1537540292885886 2311 88-89 3.2921380433796195 2311 90-91 3.403372505299469 2311 92-93 3.7244439336096526 2311 94-95 3.6821393787681806 2311 96-97 3.59134974006 2311 98-99 3.886875431294042 2311 100-101 3.686970570653166 2312 1 -0.2971543147978757 2312 2 -0.15774833971351754 2312 3 -0.19975155719082593 2312 4 -0.24313886711004074 2312 5 -0.21938564669407157 2312 6 -0.08275722422568066 2312 7 -0.21211857844625115 2312 8 -0.16952452927031914 2312 9 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1.695610067092609 2312 74-75 1.7346441984315568 2312 76-77 1.9295163343614554 2312 78-79 1.943669072994357 2312 80-81 2.2111632086632866 2312 82-83 2.3624180438447944 2312 84-85 2.53579234774735 2312 86-87 2.6688349399194706 2312 88-89 2.786152683263289 2312 90-91 2.9212325031897706 2312 92-93 3.103606259213535 2312 94-95 3.124469444168014 2312 96-97 3.1189757672767193 2312 98-99 3.193925632018006 2312 100-101 3.062090250011355 2313 1 -0.4219289641031452 2313 2 -0.5101575859027783 2313 3 -0.3959037171963331 2313 4 -0.33930817065456864 2313 5 -0.27228624987003514 2313 6 -0.2433861540925193 2313 7 -0.3313060583234986 2313 8 -0.2128033613739575 2313 9 -0.3210292151067975 2313 10-11 -0.254732577930433 2313 12-13 -0.313599747910807 2313 14-15 -0.16409903559475225 2313 16-17 -0.09582635180026955 2313 18-19 -0.08439185982212649 2313 20-21 -0.0549454146343038 2313 22-23 -0.26919433342697374 2313 24-25 -0.08387529706885744 2313 26-27 -0.0062503396758089025 2313 28-29 -0.1261382377809639 2313 30-31 -0.02551087991236045 2313 32-33 0.036128172664078306 2313 34-35 0.12441028849964653 2313 36-37 0.16775082014945752 2313 38-39 0.16774421608534595 2313 40-41 0.356828766534683 2313 42-43 0.43003210574720896 2313 44-45 0.5469885154815799 2313 46-47 0.43225948848211004 2313 48-49 0.5267156512447002 2313 50-51 0.6714923591036666 2313 52-53 0.48716653998103254 2313 54-55 0.6477465112506096 2313 56-57 0.6889406026662108 2313 58-59 0.9385307114039279 2313 60-61 1.0384950884815929 2313 62-63 1.13588119010139 2313 64-65 1.465334285731327 2313 66-67 1.4736071540202431 2313 68-69 1.736837788895695 2313 70-71 1.8648640329838884 2313 72-73 1.9456186710263594 2313 74-75 2.0563586295282015 2313 76-77 2.1747694958648474 2313 78-79 2.230863034287271 2313 80-81 2.429663764943786 2313 82-83 2.543926348811951 2313 84-85 2.6272891523910964 2313 86-87 2.815248723839474 2313 88-89 2.705789248277025 2313 90-91 2.899551112806943 2313 92-93 3.0016094389701315 2313 94-95 3.0994346907085095 2313 96-97 3.01618356562496 2313 98-99 2.730322322253551 2313 100-101 2.5010777455204227 2314 1 -0.4649240361335849 2314 2 -0.5606325946051207 2314 3 -0.5775722905464278 2314 4 -0.34907732611048203 2314 5 -0.2737717208783792 2314 6 -0.31269873158650086 2314 7 -0.3396772456358903 2314 8 -0.28940081207588264 2314 9 -0.3671674379883356 2314 10-11 -0.43451600394488565 2314 12-13 -0.49529756879380926 2314 14-15 -0.4584637472156814 2314 16-17 -0.38658790321727565 2314 18-19 -0.5232842541530331 2314 20-21 -0.35789999081837465 2314 22-23 -0.3725333388638248 2314 24-25 -0.4394218519492199 2314 26-27 -0.27461003894084257 2314 28-29 -0.24870330075585656 2314 30-31 -0.27118180553124915 2314 32-33 -0.17755985652846817 2314 34-35 -0.1479685092445564 2314 36-37 -0.18057718789537347 2314 38-39 -0.04146902048500323 2314 40-41 0.010092871117471702 2314 42-43 0.22715791233183324 2314 44-45 0.27209638773474154 2314 46-47 0.24157883925720114 2314 48-49 0.24144164820998526 2314 50-51 0.17881922569559805 2314 52-53 0.294913636084587 2314 54-55 0.23488238346158852 2314 56-57 0.29971675227804795 2314 58-59 0.5938959235891836 2314 60-61 0.7368053881840986 2314 62-63 0.8816502068312992 2314 64-65 0.9889318905022186 2314 66-67 1.393726547883503 2314 68-69 1.4990252997836073 2314 70-71 1.59396619126003 2314 72-73 1.5899258385258612 2314 74-75 1.724226682559891 2314 76-77 1.800944032035737 2314 78-79 2.1060281310166964 2314 80-81 2.2835560761669456 2314 82-83 2.5083888782472776 2314 84-85 2.770574574110153 2314 86-87 2.7331214117261062 2314 88-89 3.080624231458895 2314 90-91 2.986104831255169 2314 92-93 2.994234106179558 2314 94-95 3.0535280107957483 2314 96-97 3.214763486026918 2314 98-99 3.2845567976471077 2314 100-101 3.0379603229089156 2315 1 -0.5996585540560879 2315 2 -0.5323740501283041 2315 3 -0.6143369161933521 2315 4 -0.4530783072149376 2315 5 -0.26923427563594515 2315 6 -0.3886698320016606 2315 7 -0.33622391755987024 2315 8 -0.3278326149742128 2315 9 -0.6898098434922915 2315 10-11 -0.5060782010651153 2315 12-13 -0.37417901036588574 2315 14-15 -0.5091350887864436 2315 16-17 -0.4605092542749887 2315 18-19 -0.4847165803396436 2315 20-21 -0.3954796383732102 2315 22-23 -0.4008214027932624 2315 24-25 -0.43768122215637817 2315 26-27 -0.5077533728621262 2315 28-29 -0.4756800413815725 2315 30-31 -0.46795965752161095 2315 32-33 -0.44177715781043503 2315 34-35 -0.5168971314524029 2315 36-37 -0.2473510855005756 2315 38-39 -0.2700933537321859 2315 40-41 -0.35929596953595677 2315 42-43 -0.13136443100292894 2315 44-45 -0.11972906915716663 2315 46-47 0.008713395355485432 2315 48-49 -0.259679398809574 2315 50-51 0.009416614726475814 2315 52-53 0.2600783204283985 2315 54-55 0.09557271683025448 2315 56-57 0.30870996549123575 2315 58-59 0.5961320730212947 2315 60-61 0.7342018538953603 2315 62-63 0.8173417331930288 2315 64-65 1.014342398189573 2315 66-67 1.151795822587701 2315 68-69 1.3618862290413176 2315 70-71 1.5560776277230097 2315 72-73 1.7473550337775379 2315 74-75 1.9553663989027896 2315 76-77 1.9606268848086685 2315 78-79 2.162986627399196 2315 80-81 2.37122673321214 2315 82-83 2.4628402406942023 2315 84-85 2.629175622413097 2315 86-87 2.93018305762655 2315 88-89 2.961201229673396 2315 90-91 3.094311947552363 2315 92-93 3.0514608020578606 2315 94-95 3.2459679283269445 2315 96-97 3.359688485444675 2315 98-99 3.2410215496291475 2315 100-101 3.0185186485427593 2316 1 -1.170282962051818 2316 2 -1.1130570869604952 2316 3 -1.0376745750138063 2316 4 -0.9329322999094671 2316 5 -0.9533855388505685 2316 6 -0.8658286742318424 2316 7 -0.93882438136459 2316 8 -0.8756767667886081 2316 9 -0.9482408008244363 2316 10-11 -0.9878298396240623 2316 12-13 -0.9787677068949776 2316 14-15 -1.4370196976281306 2316 16-17 -1.2232908475694089 2316 18-19 -1.1746468599289699 2316 20-21 -1.000927166381139 2316 22-23 -1.1548152200912227 2316 24-25 -1.4175694932589522 2316 26-27 -1.0959459327974557 2316 28-29 -1.1520622692773657 2316 30-31 -1.0114485802371505 2316 32-33 -0.9522660104381231 2316 34-35 -0.972238745133545 2316 36-37 -1.0860506766774023 2316 38-39 -1.0807294759273063 2316 40-41 -1.2660689916973311 2316 42-43 -0.979617405994496 2316 44-45 -0.9141903758943073 2316 46-47 -0.6425980472024122 2316 48-49 -0.36851615125107884 2316 50-51 -0.3070071147098794 2316 52-53 -0.3006018618544779 2316 54-55 -0.09976462013040788 2316 56-57 0.0700194585178302 2316 58-59 0.22053777218513915 2316 60-61 0.26972432147802294 2316 62-63 0.46551113011079437 2316 64-65 0.509626097522176 2316 66-67 0.6533181921834519 2316 68-69 0.8921966774403209 2316 70-71 0.6098722730103852 2316 72-73 0.9682992771824779 2316 74-75 1.0664247834524296 2316 76-77 1.156193878597385 2316 78-79 1.3264116170561344 2316 80-81 1.6517192909699823 2316 82-83 1.935416918856184 2316 84-85 1.9449768967873418 2316 86-87 2.238190119488955 2316 88-89 2.1076533038658205 2316 90-91 2.2953386496667036 2316 92-93 2.354841979996653 2316 94-95 2.2594671566800066 2316 96-97 2.2107560324511724 2316 98-99 2.142592249228491 2316 100-101 2.1520882505525805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11425.0 3 4448.0 4 5038.0 5 5782.0 6 6596.0 7 8346.0 8 10753.0 9 13425.0 10 16897.0 11 20803.0 12 25052.0 13 29113.0 14 32531.0 15 37794.0 16 42760.0 17 47671.0 18 48277.0 19 48614.0 20 52299.0 21 56610.0 22 63161.0 23 70086.0 24 76952.0 25 85435.0 26 95229.0 27 104862.0 28 116248.0 29 127840.0 30 142080.0 31 159594.0 32 180480.0 33 206052.0 34 238401.0 35 277947.0 36 317920.0 37 313586.0 38 160572.0 39 10099.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59449570766757 18.123283588313864 14.39280575892534 23.889414945093222 2 15.789011698837886 19.36668276425455 39.938635464872135 24.90567007203543 3 19.65617439338837 23.089077779035232 28.772492728001247 28.48225509957515 4 13.11296648279482 16.005048166185727 35.91756170881526 34.96442364220418 5 14.454250574443291 36.82656447271292 33.52491130583327 15.194273647010522 6 33.65946359515632 34.93728809359414 17.052976333784766 14.350271977464768 7 30.130235929335885 31.45167875443256 19.71631596089711 18.701769355334445 8 27.037729703444196 34.6598546352041 19.15882319658807 19.14359246476364 9 26.620963324260565 16.713886045259034 19.262447885618894 37.40270274486151 10-11 25.58102657036781 26.034914274066395 26.874091196226992 21.509967959338805 12-13 26.44615878194771 23.154902973655826 27.144499505914798 23.254438738481667 14-15 22.965181755113072 24.87447171234545 25.24347602305344 26.91687050948804 16-17 23.035796333289756 27.9837527780135 25.974775981450094 23.005674907246647 18-19 23.084695515540513 26.523905506436364 27.964239066379566 22.427159911643557 20-21 23.41854404170909 25.82348142821707 28.21722445276851 22.540750077305326 22-23 23.14765456384359 25.663165769192776 28.098991569036368 23.09018809792726 24-25 23.02465969148543 25.844267041580366 28.183653872920285 22.947419394013924 26-27 22.559533131535094 26.26457816908474 27.997762192928576 23.178126506451587 28-29 22.70594773378812 26.650978381914715 27.927874059001123 22.715199825296047 30-31 22.726718021401137 26.085043611559527 27.899806272797505 23.28843209424183 32-33 22.473656145742236 26.11860358036946 27.916281372808832 23.491458901079476 34-35 23.29746637520462 26.2192892516108 27.602388276069302 22.880856097115277 36-37 23.352348446793236 25.774416682531708 27.65299061783445 23.220244252840608 38-39 22.752990790827432 26.15211747057907 27.65230240017051 23.442589338422987 40-41 23.25670217481347 25.95154682979284 27.74418049601728 23.047570499376413 42-43 23.036399053251543 25.979682827034928 27.5403499544362 23.443568165277327 44-45 22.930332727869523 26.265423694972213 27.650641928723037 23.153601648435227 46-47 23.25093842314333 26.55468020529951 27.045942866997947 23.148438504559213 48-49 23.149583618473798 26.451785422702013 27.100422650029753 23.298208308794436 50-51 23.29897083735977 26.657282985557725 26.972273805969383 23.07147237111312 52-53 23.535457568372728 26.404194555742556 26.970860728109542 23.08948714777517 54-55 22.9512745484732 26.522976117312886 27.255707720672547 23.27004161354137 56-57 23.048223363058224 26.688790004658163 27.0920030508131 23.170983581470512 58-59 23.073896065894665 26.175558078404475 27.153022745694688 23.597523110006165 60-61 22.790151578850814 26.468281571283335 27.414399608631513 23.32716724123434 62-63 22.896073698226797 26.50786468071185 27.446474461381353 23.149587159680003 64-65 22.64598047468249 26.47212693504984 27.30486836509023 23.57702422517744 66-67 22.610018468582847 26.545687079131174 27.257555325872417 23.58673912641356 68-69 23.069174409405182 26.188600497624627 27.379903150476032 23.362321942494162 70-71 22.914527489207536 26.321514779206307 27.087842296423183 23.67611543516297 72-73 22.9915036149235 26.38582260149734 27.04557213151448 23.577101652064684 74-75 22.932212254597765 26.724185246458294 26.92481687757985 23.418785621364098 76-77 23.1061404069347 26.40502793039859 26.99827944219314 23.490552220473568 78-79 23.210993115161855 26.56543118716257 26.95737851780679 23.266197179868787 80-81 23.273928014347415 26.723190211323494 26.68140830553351 23.32147346879558 82-83 23.231580728898717 26.705147184617662 26.723264237289662 23.34000784919396 84-85 23.25735194636536 26.615616632486383 26.765586936531683 23.361444484616577 86-87 23.392275142628275 26.60326103572892 26.574903538648748 23.429560282994064 88-89 23.306047553614288 26.658487609324432 26.50158303595824 23.53388180110304 90-91 23.251434245804916 26.746424974700574 26.539121063221195 23.46301971627332 92-93 23.41983438752878 26.27369187750958 26.740874932021708 23.56559880293993 94-95 23.226186735534064 26.227040697358834 26.83279549793161 23.713977069175495 96-97 23.51593614533285 26.03369054142652 26.728897771863043 23.72147554137759 98-99 23.333306832504775 26.19270546347197 26.38947411551956 24.084513588503693 100-101 23.85970475241367 25.610809053802246 26.764846854699893 23.76463933908419 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 457.0 1 331.5 2 293.0 3 395.0 4 325.5 5 302.5 6 558.0 7 1265.0 8 2076.5 9 2337.0 10 2023.5 11 1731.5 12 1770.0 13 1895.0 14 2061.5 15 2335.5 16 2508.5 17 2719.5 18 3049.5 19 3373.5 20 3866.5 21 4456.0 22 5200.0 23 6335.0 24 7854.0 25 9751.0 26 12467.0 27 15928.0 28 19384.5 29 23342.5 30 28450.5 31 35508.0 32 44419.5 33 49276.0 34 51739.0 35 57216.5 36 64198.0 37 71032.5 38 76940.0 39 81847.5 40 88054.5 41 96554.5 42 103779.0 43 109486.5 44 114927.5 45 121298.5 46 124811.0 47 127580.5 48 129586.5 49 128353.0 50 126721.0 51 123735.0 52 120861.5 53 119892.5 54 120690.5 55 115052.0 56 104607.0 57 95958.0 58 89388.0 59 80142.0 60 66505.0 61 55839.0 62 47252.5 63 37288.5 64 28407.5 65 22094.0 66 16890.5 67 12920.0 68 9817.0 69 7428.5 70 5857.5 71 4495.5 72 3545.0 73 2876.0 74 2139.5 75 1599.5 76 1210.5 77 804.5 78 541.0 79 368.5 80 222.0 81 136.5 82 88.5 83 48.5 84 26.0 85 23.5 86 19.5 87 14.0 88 10.0 89 8.0 90 7.5 91 7.0 92 6.0 93 3.5 94 1.0 95 0.5 96 1.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29393618276752503 2 0.03476237152139338 3 0.04243638669454179 4 0.04500458300746795 5 0.05995515440057381 6 0.05934367908797234 7 0.028831060989159156 8 0.03280565052106869 9 0.03934843636590438 10-11 0.03138397041927028 12-13 0.03360056842745059 14-15 0.027439954652990817 16-17 0.020805447511264903 18-19 0.025712536894891676 20-21 0.0389815511783435 22-23 0.031949585083426636 24-25 0.053779253743299 26-27 0.04604409103889044 28-29 0.038125485740701445 30-31 0.03766687925625035 32-33 0.03324897012270475 34-35 0.032270609622542405 36-37 0.030894790169189102 38-39 0.018787578979680062 40-41 0.01802323483892823 42-43 0.019506062471986788 44-45 0.008637088790495717 46-47 0.01624995643238398 48-49 0.014063932189833733 50-51 0.006313482602610144 52-53 0.004815368086736551 54-55 0.006359343251055253 56-57 0.005335122102447797 58-59 0.006879097266766499 60-61 0.0062064744229048865 62-63 0.004372048485100487 64-65 0.007796310235668701 66-67 0.00270577825826149 68-69 0.0073529906340326365 70-71 0.013452456877232266 72-73 0.013635899471012706 74-75 0.010899547447121143 76-77 0.00999762136103398 78-79 0.005671433524378603 80-81 0.00683323661832139 82-83 0.011572170290982757 84-85 0.00398987641472457 86-87 0.0011618030939427866 88-89 0.01949077558917175 90-91 0.028769913457899008 92-93 0.013223153635006715 94-95 0.022181266964618203 96-97 0.018405406909304145 98-99 0.013452456877232266 100-101 0.10865916304928062 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3270778.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.32432312004857 #Duplication Level Percentage of deduplicated Percentage of total 1 76.73995425254681 40.92106116780552 2 12.758460379949318 13.606725276295101 3 4.317689307080716 6.907135792282522 4 1.9835572921423856 4.230873998933165 5 1.0638923852865771 2.836567065899032 6 0.6734148049441104 2.154563319159853 7 0.46422524503279783 1.7328147876628845 8 0.3158764655496667 1.3475118971991455 9 0.24486926657435287 1.175173910368209 >10 1.288792048028418 12.490948598477198 >50 0.09364022801742773 3.414968575862063 >100 0.04962305803175213 4.9228240758014605 >500 0.0032913481599568866 1.209164035695685 >1k 0.002598432755497375 2.6283845261896412 >5k 1.1548590024432776E-4 0.4212829723685793 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7689 0.23508168392963386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5993 0.1832285774210295 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4298 0.1314060446780552 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3648 0.11153309701850751 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3554 0.10865916304928062 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3322 0.10156604942310361 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3278 0.10022080373538039 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.114753126014666E-5 0.0 0.0 0.0 0.0 6 6.114753126014666E-5 0.0 0.0 0.0 0.0 7 6.114753126014666E-5 0.0 0.0 3.057376563007333E-5 0.0 8 6.114753126014666E-5 0.0 0.0 3.057376563007333E-5 0.0 9 6.114753126014666E-5 0.0 0.0 3.057376563007333E-5 0.0 10-11 6.114753126014666E-5 0.0 0.0 3.057376563007333E-5 0.0 12-13 9.172129689022001E-5 0.0 0.0 6.114753126014666E-5 0.0 14-15 9.172129689022001E-5 0.0 0.0 6.114753126014666E-5 0.0 16-17 9.172129689022001E-5 0.0 0.0 7.643441407518333E-5 1.5286882815036666E-5 18-19 9.172129689022001E-5 0.0 0.0 9.172129689022001E-5 3.057376563007333E-5 20-21 9.172129689022001E-5 0.0 0.0 1.8344259378044002E-4 6.114753126014666E-5 22-23 9.172129689022001E-5 0.0 0.0 2.598770078556233E-4 6.114753126014666E-5 24-25 1.5286882815036668E-4 0.0 0.0 4.5860648445109997E-4 6.114753126014666E-5 26-27 1.8344259378044002E-4 0.0 0.0 9.172129689021999E-4 6.114753126014666E-5 28-29 1.8344259378044002E-4 0.0 0.0 0.0027822126723366734 6.114753126014666E-5 30-31 1.8344259378044002E-4 0.0 0.0 0.009569588642212953 6.114753126014666E-5 32-33 1.8344259378044002E-4 0.0 0.0 0.024199135496203044 6.114753126014666E-5 34-35 1.9872947659547668E-4 0.0 0.0 0.046594418820231756 6.114753126014666E-5 36-37 2.4459012504058665E-4 0.0 0.0 0.08152494605259054 6.114753126014666E-5 38-39 2.7516389067066E-4 0.0 0.0 0.13852973206986227 6.114753126014666E-5 40-41 2.7516389067066E-4 0.0 0.0 0.1991575093142977 6.114753126014666E-5 42-43 2.7516389067066E-4 0.0 0.0 0.26272036805922017 6.114753126014666E-5 44-45 2.7516389067066E-4 0.0 0.0 0.3460797400496151 6.114753126014666E-5 46-47 2.7516389067066E-4 0.0 0.0 0.4405985364949868 6.114753126014666E-5 48-49 2.7516389067066E-4 0.0 0.0 0.540345446863101 6.114753126014666E-5 50-51 2.7516389067066E-4 0.0 0.0 0.6563728874292294 6.114753126014666E-5 52-53 2.7516389067066E-4 0.0 0.0 0.7677072549711415 7.643441407518333E-5 54-55 2.7516389067066E-4 0.0 0.0 0.8913169894135279 9.172129689022001E-5 56-57 2.7516389067066E-4 0.0 0.0 1.038116925086325 9.172129689022001E-5 58-59 2.7516389067066E-4 0.0 0.0 1.1954953836671276 9.172129689022001E-5 60-61 2.7516389067066E-4 0.0 0.0 1.366555602367388 1.2229506252029333E-4 62-63 2.9045077348569665E-4 0.0 0.0 1.533794100363889 1.5286882815036668E-4 64-65 3.0573765630073337E-4 0.0 0.0 1.7132468177296043 1.5286882815036668E-4 66-67 3.0573765630073337E-4 0.0 0.0 1.9239306366864397 1.5286882815036668E-4 68-69 3.6688518756088003E-4 0.0 0.0 2.154013509935557 1.5286882815036668E-4 70-71 3.6688518756088003E-4 0.0 0.0 2.4037094538363655 1.8344259378044002E-4 72-73 3.821720703759167E-4 0.0 0.0 2.659428429566299 1.8344259378044002E-4 74-75 3.9745895319095336E-4 0.0 0.0 2.919045560414067 1.8344259378044002E-4 76-77 3.9745895319095336E-4 0.0 0.0 3.204803260875547 1.8344259378044002E-4 78-79 3.9745895319095336E-4 0.0 0.0 3.5224188251235637 1.8344259378044002E-4 80-81 4.586064844511E-4 0.0 0.0 3.8616041810235973 1.8344259378044002E-4 82-83 4.586064844511E-4 0.0 0.0 4.209243183120346 1.9872947659547668E-4 84-85 4.7389336726613664E-4 0.0 0.0 4.563791856249491 2.1401635941051335E-4 86-87 4.891802500811733E-4 0.0 0.0 4.929270650591389 2.1401635941051335E-4 88-89 4.891802500811733E-4 0.0 0.0 5.315203294139804 2.1401635941051335E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4720 0.0 49.261066 1 GTATCAA 8595 0.0 43.62695 1 ATCAACG 10960 0.0 33.647495 3 TATCAAC 11140 0.0 33.274456 2 TCAACGC 11135 0.0 33.118687 4 CAACGCA 11365 0.0 32.406628 5 AACGCAG 11765 0.0 31.341389 6 ACGCAGA 13705 0.0 26.724983 7 CGCAGAG 13860 0.0 26.289013 8 GTACATG 11515 0.0 26.274582 1 TACATGG 11505 0.0 25.403296 2 TGGTATC 2395 0.0 25.199955 2 GTGGTAT 2625 0.0 24.140615 1 GCAGAGT 15225 0.0 23.464035 9 ACATGGG 12110 0.0 23.388573 3 GAGTACT 8575 0.0 21.187984 12-13 TAAGGTG 1860 0.0 20.950891 5 AGAGTAC 13910 0.0 20.557405 10-11 GTGTAAG 1370 0.0 20.519058 1 GGACCGA 1055 0.0 20.267887 6 >>END_MODULE