FastQCFastQC Report
Thu 26 May 2016
SRR938429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270778
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77560.2371301262268488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64430.19698677195456252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47240.14443046883646643No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36640.1120222772685887No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35350.10807826150230923No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34480.10541834389249286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50650.051.6487961
GTATCAA88150.042.0871241
ATCAACG115800.031.656043
TATCAAC116650.031.4781742
TCAACGC117250.031.2240094
CAACGCA119700.030.54525
AACGCAG124700.029.3271646
ACGCAGA145100.025.1712477
CGCAGAG147900.024.6304878
GTACATG112100.023.930431
GTAAGGT18150.023.5763554
TAAGGTG19650.023.4703715
TACATGG111450.023.2591272
GTGGTAT26100.022.232911
ACATGGG114250.021.9729563
GCAGAGT162800.021.9094439
GAGTACT95450.020.62502112-13
TGGTATC24750.020.5629582
GTACTTT96450.020.01723714-15
AGTACTT98400.020.0066912-13
Produced by FastQC (version 0.11.5)