##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938429_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3270778 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.805524251416635 31.0 31.0 34.0 30.0 34.0 2 32.045400819010034 33.0 31.0 34.0 30.0 34.0 3 32.11505274891784 33.0 31.0 34.0 30.0 34.0 4 35.776487734722444 37.0 35.0 37.0 35.0 37.0 5 35.646054547266736 37.0 35.0 37.0 33.0 37.0 6 35.68423261988432 37.0 35.0 37.0 33.0 37.0 7 35.62120480203792 37.0 35.0 37.0 33.0 37.0 8 35.60340689585169 37.0 35.0 37.0 33.0 37.0 9 37.17927538952506 39.0 37.0 39.0 33.0 39.0 10-11 37.11172938059386 39.0 37.0 39.0 33.0 39.0 12-13 37.00016142948253 39.0 37.0 39.0 33.0 39.0 14-15 38.0598982871965 40.0 37.0 41.0 33.0 41.0 16-17 37.97640576645679 40.0 37.0 41.0 33.0 41.0 18-19 37.95983967728779 40.0 37.0 41.0 33.0 41.0 20-21 37.948544199575764 40.0 37.0 41.0 32.5 41.0 22-23 37.98844464528011 40.0 37.0 41.0 33.0 41.0 24-25 37.90872829033337 40.0 37.0 41.0 32.5 41.0 26-27 37.733273245692615 40.0 37.0 41.0 32.0 41.0 28-29 37.602189754241955 40.0 37.0 41.0 32.0 41.0 30-31 37.37080459144583 39.0 36.0 41.0 31.5 41.0 32-33 37.16142887105147 39.0 36.0 41.0 31.0 41.0 34-35 36.99551574579504 39.0 36.0 41.0 31.0 41.0 36-37 37.01007497298808 39.0 36.0 41.0 31.0 41.0 38-39 36.86178533058496 39.0 35.5 41.0 30.0 41.0 40-41 36.68746701855032 39.0 35.0 41.0 30.0 41.0 42-43 36.464337383949626 39.0 35.0 40.0 30.0 41.0 44-45 36.19318920452565 39.0 35.0 40.0 30.0 41.0 46-47 35.85044995410878 38.0 35.0 40.0 28.5 41.0 48-49 35.46436352451924 38.0 34.0 40.0 27.5 41.0 50-51 35.08999143323087 38.0 34.0 40.0 26.5 41.0 52-53 34.56445912868436 38.0 33.0 40.0 25.5 41.0 54-55 34.229591399966615 37.5 33.0 40.0 24.0 41.0 56-57 33.851136182278346 37.0 33.0 40.0 23.0 41.0 58-59 33.37153163559251 36.5 32.0 39.0 22.5 41.0 60-61 32.9464939228526 36.0 31.0 39.0 22.0 40.0 62-63 32.44993545878076 35.5 31.0 39.0 20.0 40.0 64-65 31.931515682201606 35.0 30.5 38.5 18.0 40.0 66-67 31.418115812201258 35.0 30.0 38.0 16.5 40.0 68-69 30.838873656359436 34.0 29.0 37.5 12.5 39.5 70-71 30.2538735432365 34.0 29.0 37.0 8.0 39.0 72-73 29.64962128276514 34.0 28.0 36.0 7.0 39.0 74-75 29.012481739818476 33.5 27.5 36.0 4.0 38.5 76-77 27.73464264465519 31.5 26.0 34.5 2.0 36.0 78-79 28.34094747488212 33.0 27.0 35.0 2.0 37.0 80-81 28.023051702072106 33.0 26.0 35.0 2.0 36.5 82-83 27.50512599754554 33.0 26.0 35.0 2.0 36.0 84-85 26.90257027532899 32.0 25.0 35.0 2.0 36.0 86-87 26.23802823059223 32.0 24.5 34.5 2.0 35.0 88-89 25.472886878901598 31.0 21.0 34.0 2.0 35.0 90-91 24.90496358970251 31.0 19.0 34.0 2.0 35.0 92-93 24.104910055038893 31.0 14.0 34.0 2.0 35.0 94-95 23.295408003844955 30.5 4.5 34.0 2.0 35.0 96-97 22.29714673389634 30.0 2.0 34.0 2.0 35.0 98-99 21.09499605292686 29.0 2.0 34.0 2.0 35.0 100-101 17.951389241336464 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.349730858668881 1101 2 0.3316143542224026 1101 3 0.34517997732289984 1101 4 0.2298138735312989 1101 5 0.20960939386212374 1101 6 0.21092903108889516 1101 7 0.2812774157220943 1101 8 0.2887452444197507 1101 9 0.45410381235128483 1101 10-11 0.3701284402217553 1101 12-13 0.4172461594805057 1101 14-15 0.5445010453702679 1101 16-17 0.5143234971555373 1101 18-19 0.6802496645243181 1101 20-21 0.571668626166506 1101 22-23 0.68341145022071 1101 24-25 0.5925506732312513 1101 26-27 0.5178005385518674 1101 28-29 0.6103318363783501 1101 30-31 0.6384635033463937 1101 32-33 0.6097360985277334 1101 34-35 0.43961643197440026 1101 36-37 0.6129130006698205 1101 38-39 0.7982568554404423 1101 40-41 0.9020027448923074 1101 42-43 0.8749467963028721 1101 44-45 0.9193955053623526 1101 46-47 1.0735583769181218 1101 48-49 1.107142750107485 1101 50-51 1.1937137245338292 1101 52-53 1.2832744768233582 1101 54-55 1.3234005561724302 1101 56-57 1.3794387622850621 1101 58-59 1.4488485272191767 1101 60-61 1.5130482189415488 1101 62-63 1.6889980961020967 1101 64-65 1.7457911528792742 1101 66-67 1.7972341120052313 1101 68-69 1.8965588185657545 1101 70-71 1.9302686250163035 1101 72-73 1.9716934050270858 1101 74-75 2.0828829638681405 1101 76-77 1.8228265525995866 1101 78-79 2.1563586887638735 1101 80-81 2.5361326149378804 1101 82-83 2.7016420285130955 1101 84-85 2.8729754272357546 1101 86-87 2.951945960472049 1101 88-89 3.1332014003921422 1101 90-91 3.322861200509397 1101 92-93 3.5976569634711595 1101 94-95 3.6527934388661585 1101 96-97 3.907175736960429 1101 98-99 4.107475350918577 1101 100-101 4.523268377078143 1102 1 0.2660334860826232 1102 2 0.25553088997694573 1102 3 0.27347178858460097 1102 4 0.1437068303110678 1102 5 0.11540502894124671 1102 6 0.06589496435181275 1102 7 0.18319834775725496 1102 8 0.20096666785612882 1102 9 0.18991989043112056 1102 10-11 0.2312281612383984 1102 12-13 0.2399411445846269 1102 14-15 0.3497728840273595 1102 16-17 0.28714051898605675 1102 18-19 0.43295592138425576 1102 20-21 0.4805795260431367 1102 22-23 0.3573436658455549 1102 24-25 0.3492860650652929 1102 26-27 0.3192161645780871 1102 28-29 0.3360169431641822 1102 30-31 0.30622790885743 1102 32-33 0.18100362374125467 1102 34-35 0.3404715765990147 1102 36-37 0.401208717106762 1102 38-39 0.41491597801856983 1102 40-41 0.4351260271329309 1102 42-43 0.4998962804943119 1102 44-45 0.3780158838685068 1102 46-47 0.4191955269685579 1102 48-49 0.5243659042465296 1102 50-51 0.6889963024228507 1102 52-53 0.7646780027232509 1102 54-55 0.5568534115359824 1102 56-57 0.7147933196541629 1102 58-59 0.7618724345836867 1102 60-61 0.8927469287544838 1102 62-63 0.9430508988049482 1102 64-65 0.9600030323040336 1102 66-67 1.0106466138912502 1102 68-69 1.1012860036148524 1102 70-71 1.3062534244175126 1102 72-73 1.395435539205355 1102 74-75 1.4971764969571062 1102 76-77 1.5702705858172408 1102 78-79 1.9069063008379672 1102 80-81 2.148062481540645 1102 82-83 2.091667412152187 1102 84-85 2.207995382089564 1102 86-87 2.4551561920311933 1102 88-89 2.483782443133105 1102 90-91 2.721628999381039 1102 92-93 2.9894034347607246 1102 94-95 3.0889563970955756 1102 96-97 3.546462687462334 1102 98-99 3.8697773925560313 1102 100-101 4.456890752548912 1103 1 0.3183634544725429 1103 2 0.36297807866250764 1103 3 0.2980698012572205 1103 4 0.22679443986709202 1103 5 0.18923642345346536 1103 6 0.19065822233366703 1103 7 0.3204767937270887 1103 8 0.3500123142521332 1103 9 0.34915271001736414 1103 10-11 0.39855838216585227 1103 12-13 0.4812976427563527 1103 14-15 0.6379696136187505 1103 16-17 0.6073494294629356 1103 18-19 0.6841669102926105 1103 20-21 0.6652958596938134 1103 22-23 0.5389586004383276 1103 24-25 0.5503915123473675 1103 26-27 0.5828222670282699 1103 28-29 0.4864911895779116 1103 30-31 0.6327959524730389 1103 32-33 0.6523500966496982 1103 34-35 0.660631742568583 1103 36-37 0.7450108267957205 1103 38-39 0.8424949817627905 1103 40-41 0.8034526753691225 1103 42-43 0.8541043180490249 1103 44-45 0.9956726882300657 1103 46-47 1.009177458623327 1103 48-49 1.1817562958131091 1103 50-51 1.10772939312956 1103 52-53 1.233643570899325 1103 54-55 1.3225440444903143 1103 56-57 1.3422755967612119 1103 58-59 1.5762559714241462 1103 60-61 1.5626143417886595 1103 62-63 1.7594852309480231 1103 64-65 1.7900765350185672 1103 66-67 1.7814467957182707 1103 68-69 1.8827911558568609 1103 70-71 1.9966421797315235 1103 72-73 2.0545984583104406 1103 74-75 2.099237107869204 1103 76-77 2.109332518501219 1103 78-79 2.2675479462771904 1103 80-81 2.585538716806507 1103 82-83 2.6151429669961885 1103 84-85 2.8857067059675074 1103 86-87 3.1186668067075267 1103 88-89 3.4001845648295053 1103 90-91 3.581544432663957 1103 92-93 3.6369247074736144 1103 94-95 3.588422494647485 1103 96-97 4.102370779050549 1103 98-99 4.479531630659846 1103 100-101 4.65401868615346 1104 1 0.25046390387642603 1104 2 0.2551673955993152 1104 3 0.1656282878022708 1104 4 0.16268747297137764 1104 5 0.03649500644055337 1104 6 0.15650225839741694 1104 7 0.18960865818819173 1104 8 0.2713814840442694 1104 9 0.3307158139006674 1104 10-11 0.2558280786515823 1104 12-13 0.311695328000404 1104 14-15 0.43427395007153535 1104 16-17 0.3820560922200613 1104 18-19 0.4193712319696985 1104 20-21 0.502551834176252 1104 22-23 0.3831153891291308 1104 24-25 0.42956607371724687 1104 26-27 0.4391476793159015 1104 28-29 0.44639956734638986 1104 30-31 0.3999396527596275 1104 32-33 0.4330365517249817 1104 34-35 0.521128141829962 1104 36-37 0.6050546224577644 1104 38-39 0.5732554210912468 1104 40-41 0.5752685596269131 1104 42-43 0.6654473228018034 1104 44-45 0.6737245995872527 1104 46-47 0.7874188428575337 1104 48-49 0.8870103960707141 1104 50-51 1.011647142757802 1104 52-53 1.1980819843069455 1104 54-55 1.2729308746036594 1104 56-57 1.3816282499469565 1104 58-59 1.5372195660961765 1104 60-61 1.4325506867357731 1104 62-63 1.5864950782599365 1104 64-65 1.5750973189252484 1104 66-67 1.710953084034994 1104 68-69 1.6355262469931553 1104 70-71 1.6739438586725015 1104 72-73 1.9162183442920302 1104 74-75 1.9409056571277894 1104 76-77 1.8169484326046437 1104 78-79 2.214279421917553 1104 80-81 2.474003030265461 1104 82-83 2.628213625658784 1104 84-85 2.7231705700869426 1104 86-87 2.865957368472035 1104 88-89 2.9676742206880284 1104 90-91 3.088256619897404 1104 92-93 3.2507775221622985 1104 94-95 3.4105358560114425 1104 96-97 3.7341302839330126 1104 98-99 4.029846945266929 1104 100-101 4.251524856411667 1105 1 0.30867736744188434 1105 2 0.35648199115054524 1105 3 0.31517852045704586 1105 4 0.23014982940971862 1105 5 0.17102306112926158 1105 6 0.18236970353787996 1105 7 0.20785520051371975 1105 8 0.22719620614708447 1105 9 0.3295926132312985 1105 10-11 0.35354250742747695 1105 12-13 0.3478732292419906 1105 14-15 0.4829524734439019 1105 16-17 0.5387554267305248 1105 18-19 0.5322258443011876 1105 20-21 0.364500482394547 1105 22-23 0.47952666424661317 1105 24-25 0.4112754287398701 1105 26-27 0.46441265389311326 1105 28-29 0.5173971321361108 1105 30-31 0.5687243979888805 1105 32-33 0.6447757743386973 1105 34-35 0.5636448693990275 1105 36-37 0.6742633287817554 1105 38-39 0.7903669754723879 1105 40-41 0.6937404594185992 1105 42-43 0.7503043673325465 1105 44-45 0.8424476188158323 1105 46-47 0.844950707909085 1105 48-49 0.8378687505742093 1105 50-51 0.8784596581339486 1105 52-53 1.1299632688241346 1105 54-55 1.0907360007487554 1105 56-57 1.0986041311099655 1105 58-59 1.3193789010069352 1105 60-61 1.350629081059175 1105 62-63 1.443355449441313 1105 64-65 1.5577472648419395 1105 66-67 1.585702925469132 1105 68-69 1.7250823493743646 1105 70-71 1.7945656741820741 1105 72-73 1.895774418334181 1105 74-75 2.0634127724441775 1105 76-77 1.850296635085055 1105 78-79 2.142476585213732 1105 80-81 2.3659579980926644 1105 82-83 2.512993942202467 1105 84-85 2.48909038756927 1105 86-87 2.7057828246997992 1105 88-89 2.8159449362260993 1105 90-91 3.036652487780028 1105 92-93 3.275366477476748 1105 94-95 3.3769839223049907 1105 96-97 3.7828768653132947 1105 98-99 4.346062737594096 1105 100-101 4.874173312756863 1106 1 0.14040315056544728 1106 2 0.18241048564192397 1106 3 0.17708579261829982 1106 4 0.14923767774102004 1106 5 0.021899586041143948 1106 6 0.03023492342105527 1106 7 0.13957350801990742 1106 8 0.022637518514528665 1106 9 0.13874308477429054 1106 10-11 0.13648032327669313 1106 12-13 0.05616130233317307 1106 14-15 0.10471194247151061 1106 16-17 0.07444790229350673 1106 18-19 0.24588881609424362 1106 20-21 0.4019258622208355 1106 22-23 0.16260751708469456 1106 24-25 0.13290791756242015 1106 26-27 0.17248988298709378 1106 28-29 0.1929346307804991 1106 30-31 0.2748963975550822 1106 32-33 0.3490065701620395 1106 34-35 0.293148867450455 1106 36-37 0.3447060888919964 1106 38-39 0.3915382257220088 1106 40-41 0.3894387856173438 1106 42-43 0.5198567042000093 1106 44-45 0.020588509986630754 1106 46-47 -0.050283691302219324 1106 48-49 0.36936979136967096 1106 50-51 0.36520386181048536 1106 52-53 0.5547173192575272 1106 54-55 0.6737249175317928 1106 56-57 0.541493309820396 1106 58-59 0.9626554254976512 1106 60-61 0.9089989998767791 1106 62-63 1.0742641265272894 1106 64-65 0.7907286533623967 1106 66-67 0.7672429412556703 1106 68-69 0.7065220234234104 1106 70-71 0.9427721577534456 1106 72-73 1.3719067550121231 1106 74-75 1.5449230787935733 1106 76-77 1.6952195151421066 1106 78-79 1.921860499580358 1106 80-81 1.85880764026329 1106 82-83 2.11558073394475 1106 84-85 2.111670244184822 1106 86-87 2.333934911748276 1106 88-89 2.833785690133336 1106 90-91 2.6638080890706064 1106 92-93 2.441032037307483 1106 94-95 3.0217866309352566 1106 96-97 3.4222034209875183 1106 98-99 3.5445026499503776 1106 100-101 3.8820144632578852 1107 1 0.2601482955375154 1107 2 0.21971932066794153 1107 3 0.24226999967422103 1107 4 0.17095931102663542 1107 5 0.12947421396814462 1107 6 0.14944627605412109 1107 7 0.1432120531190293 1107 8 0.20520225581164198 1107 9 0.27931311258872427 1107 10-11 0.26985609222469265 1107 12-13 0.24568387150078053 1107 14-15 0.47591383285315914 1107 16-17 0.48706162298901035 1107 18-19 0.44738667549473377 1107 20-21 0.4881084583323343 1107 22-23 0.39213387011855616 1107 24-25 0.41709424410059626 1107 26-27 0.40327241066146513 1107 28-29 0.46253501485584536 1107 30-31 0.45850281857786257 1107 32-33 0.5584779149170771 1107 34-35 0.5614207179851576 1107 36-37 0.580746251313812 1107 38-39 0.6418244721870252 1107 40-41 0.728766558937032 1107 42-43 0.8296614860071116 1107 44-45 0.8572448220185507 1107 46-47 0.959314141464688 1107 48-49 1.021337875880036 1107 50-51 0.9679578958514838 1107 52-53 1.0531440842070268 1107 54-55 1.0300607817746013 1107 56-57 1.0642092638589133 1107 58-59 1.1235558046600076 1107 60-61 1.1611060272662854 1107 62-63 1.3253013282245902 1107 64-65 1.3565176325718262 1107 66-67 1.563651198875764 1107 68-69 1.6884153614916038 1107 70-71 1.6243789907780624 1107 72-73 1.9692066776971338 1107 74-75 1.8906389492250284 1107 76-77 1.8332174189319232 1107 78-79 2.0537811176865475 1107 80-81 2.23917045183968 1107 82-83 2.3511498093281595 1107 84-85 2.5523520641296784 1107 86-87 2.853922258637443 1107 88-89 3.0670346262980495 1107 90-91 3.1269740904720997 1107 92-93 3.3817654005784696 1107 94-95 3.5030082250031214 1107 96-97 3.896815014443412 1107 98-99 4.019080628193578 1107 100-101 4.196635400019051 1108 1 0.06203386519360521 1108 2 0.12320592100115846 1108 3 0.07855001565613406 1108 4 0.07546835887238501 1108 5 -0.035662992249989145 1108 6 -0.012218196734039566 1108 7 0.028580412796046062 1108 8 0.0213918036230325 1108 9 0.036179225374787904 1108 10-11 0.07326545761018366 1108 12-13 0.11885026158376633 1108 14-15 0.18392631059718667 1108 16-17 0.18526834847823892 1108 18-19 0.1756082101782468 1108 20-21 0.23322971555433725 1108 22-23 0.27868774715245337 1108 24-25 0.12548693011152778 1108 26-27 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0.7956256808708844 2316 80-81 1.0520738441751014 2316 82-83 1.2103862778725158 2316 84-85 1.392918400516649 2316 86-87 1.62615649327142 2316 88-89 1.7704278576690449 2316 90-91 1.85384471309996 2316 92-93 1.8084640428251468 2316 94-95 2.3918268123662507 2316 96-97 2.557245618162387 2316 98-99 2.638341321610284 2316 100-101 3.269717249198532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 15.0 4 49.0 5 104.0 6 218.0 7 578.0 8 1336.0 9 2515.0 10 4040.0 11 6017.0 12 8820.0 13 12640.0 14 16387.0 15 21613.0 16 26751.0 17 33157.0 18 32920.0 19 30880.0 20 31793.0 21 34411.0 22 37397.0 23 42158.0 24 48513.0 25 56597.0 26 66308.0 27 78939.0 28 93332.0 29 110819.0 30 129834.0 31 152558.0 32 181975.0 33 220497.0 34 272297.0 35 342173.0 36 430382.0 37 476981.0 38 252802.0 39 12963.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57834068836222 18.922653876233728 15.009211875584342 23.489793559819713 2 16.289609506954587 20.190410704989425 38.89019053917073 24.629789248885253 3 19.73498595132033 23.36987926390422 29.1937984022111 27.70133638256435 4 13.693622740522285 16.724063816009522 35.56459655776087 34.017716885707316 5 14.339065033608522 36.93543282794554 33.75028999248927 14.975212145956663 6 33.65067882931829 35.51980599111282 16.67667447928291 14.152840700285987 7 30.089446608727343 31.607648088619893 19.65248023558921 18.650425067063555 8 26.997552264323655 35.12353941478143 18.963500427115505 18.915407893779403 9 26.38332531281548 16.72632627466615 19.31103242103255 37.57931599148582 10-11 25.553828477505963 26.24795996548833 26.85385556586231 21.34435599114339 12-13 26.39141207382464 23.231980281144118 27.2511463633423 23.125461281688946 14-15 22.817507027380028 25.003622991227164 25.297085280627424 26.881784700765383 16-17 22.935017295579215 28.166631914486402 26.025230082873247 22.873120707061133 18-19 22.962426676466578 26.645617648155884 28.108037292656363 22.283918382721176 20-21 23.220438684618767 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22.97201586514171 46-47 23.23904884927884 26.592871720005196 27.158441042260627 23.009638388455336 48-49 23.14287304121527 26.490043041747253 27.197290675184927 23.16979324185255 50-51 23.32668252018327 26.641276173436413 27.114603926038395 22.917437380341923 52-53 23.58429706938227 26.42056415935291 27.049940411730788 22.94519835953403 54-55 23.042973262019007 26.525371027932803 27.28757500509053 23.14408070495766 56-57 23.09251804922254 26.695009566531265 27.17495653939216 23.03751584485404 58-59 23.079126739876568 26.262283774686026 27.17192973659478 23.48665974884263 60-61 22.80287748052604 26.53307561687158 27.443608217983613 23.220438684618767 62-63 22.8698340272559 26.64492973842921 27.438976292490658 23.04625994182424 64-65 22.578878786637308 26.52544746234688 27.31292065679786 23.58275309421795 66-67 22.558487010241322 26.63760418421478 27.276414201064032 23.527494604479866 68-69 23.00552729089369 26.24082001473353 27.410880708383544 23.342771985989234 70-71 22.838557065016335 26.407188136889754 27.08639962724465 23.667855170849258 72-73 22.87565353364866 26.503905112440425 27.027236498733558 23.593204855177355 74-75 22.780283467725415 26.86307355619978 26.810929998917686 23.545712977157116 76-77 22.826388706295567 26.549585450311824 27.070440121585754 23.55358572180686 78-79 22.975527535039063 26.634473510583724 27.08461106195529 23.305387892421926 80-81 22.96671753634038 26.827727299618303 26.889944941208917 23.315610222832404 82-83 22.90163915630649 26.834055010528974 26.881246202118025 23.383059631046514 84-85 22.85403598330351 26.763101765165587 26.894498548902533 23.488363702628366 86-87 23.020049071689 26.74668460356649 26.742358345626734 23.490907979117782 88-89 22.79582404717904 26.912157961363587 26.520033710670056 23.77198428078732 90-91 22.714546948229774 26.914696088745636 26.722170908671767 23.648586054352823 92-93 22.782647511136524 26.556911675232435 26.891511784686788 23.768929028944257 94-95 22.55232547118759 26.50627771129682 26.92885301295288 24.012543804562707 96-97 22.722850648989322 26.301509915989406 26.884735069148686 24.090904365872586 98-99 22.507962937258352 26.533656518418553 26.55686200653178 24.401518537791315 100-101 22.556596719927125 26.22820053073446 26.975444932286948 24.23975781705147 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 430.0 1 310.5 2 280.5 3 377.5 4 339.5 5 306.0 6 538.5 7 1336.5 8 2130.0 9 2345.5 10 2063.0 11 1772.5 12 1825.0 13 1949.5 14 2094.5 15 2291.5 16 2513.0 17 2724.0 18 2959.0 19 3303.0 20 3757.5 21 4341.5 22 5183.5 23 6216.0 24 7486.5 25 9540.5 26 12277.0 27 15618.0 28 19227.5 29 23420.0 30 28880.5 31 35699.0 32 45480.0 33 50537.5 34 52053.0 35 57361.5 36 64652.5 37 71900.0 38 77464.5 39 82665.5 40 89053.0 41 98089.5 42 105963.5 43 112140.5 44 117637.0 45 123830.5 46 127424.5 47 129916.0 48 131949.0 49 130249.0 50 128860.0 51 125769.0 52 122037.0 53 121350.5 54 123300.0 55 116746.0 56 104344.0 57 94764.0 58 87339.5 59 77356.5 60 63364.5 61 52920.5 62 44555.5 63 34359.5 64 25032.5 65 18699.5 66 13877.5 67 10777.0 68 8283.0 69 6141.0 70 4747.5 71 3570.0 72 2801.5 73 2343.5 74 1862.0 75 1402.0 76 948.0 77 576.5 78 365.5 79 246.5 80 153.5 81 87.5 82 48.0 83 28.5 84 18.0 85 8.0 86 3.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.04353704225722443 3 2.4459012504058665E-4 4 0.0 5 0.10554063895501316 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00128409815646308 26-27 0.011449875228462464 28-29 0.0 30-31 0.0 32-33 5.350408985262834E-4 34-35 0.015256309049406594 36-37 0.0032102453911576996 38-39 0.011618030939427867 40-41 0.00706253986054694 42-43 0.0055032778134132005 44-45 0.00261405696137127 46-47 9.172129689022E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.7516389067066E-4 68-69 2.2930324222555004E-4 70-71 0.0 72-73 4.586064844511E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.586064844511E-5 82-83 0.00123823750801797 84-85 0.004586064844511 86-87 0.0016204095783938868 88-89 1.0700817970525667E-4 90-91 0.0011312293283127133 92-93 2.4459012504058665E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.407785548270166E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3270778.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.55011462136336 #Duplication Level Percentage of deduplicated Percentage of total 1 74.59907232567122 37.70991656710055 2 13.898677831726069 14.051595149583097 3 4.635512747104642 7.0297710208479165 4 2.1509401554073264 4.3492108559813385 5 1.2090532555682967 3.0558890326154957 6 0.7482855048686227 2.269555082436818 7 0.5057530750768404 1.7896113140668892 8 0.36235247264886117 1.4653567220587491 9 0.2569212957392881 1.1688660853461188 >10 1.4651574265783487 13.477071098578769 >50 0.10429964885722275 3.639645368091377 >100 0.05694518296312034 5.333817823736294 >500 0.004120493878182603 1.4584076377347204 >1k 0.0027873929155995594 2.766355341269586 >5k 1.2119099633041564E-4 0.43493090055238337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7756 0.2371301262268488 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6443 0.19698677195456252 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4724 0.14443046883646643 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3664 0.1120222772685887 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3535 0.10807826150230923 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3448 0.10541834389249286 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.114753126014666E-5 0.0 0.0 0.0 0.0 3 6.114753126014666E-5 0.0 0.0 0.0 0.0 4 6.114753126014666E-5 0.0 0.0 0.0 0.0 5 6.114753126014666E-5 0.0 0.0 0.0 0.0 6 6.114753126014666E-5 0.0 0.0 0.0 0.0 7 6.114753126014666E-5 0.0 0.0 0.0 0.0 8 6.114753126014666E-5 0.0 0.0 0.0 0.0 9 6.114753126014666E-5 0.0 0.0 0.0 0.0 10-11 6.114753126014666E-5 0.0 0.0 0.0 0.0 12-13 6.114753126014666E-5 0.0 0.0 6.114753126014666E-5 0.0 14-15 6.114753126014666E-5 0.0 0.0 6.114753126014666E-5 0.0 16-17 6.114753126014666E-5 0.0 0.0 6.114753126014666E-5 0.0 18-19 6.114753126014666E-5 0.0 0.0 6.114753126014666E-5 0.0 20-21 6.114753126014666E-5 0.0 0.0 1.3758194533533E-4 0.0 22-23 6.114753126014666E-5 0.0 0.0 2.2930324222554998E-4 0.0 24-25 9.172129689022001E-5 0.0 0.0 4.2803271882102664E-4 0.0 26-27 1.2229506252029333E-4 0.0 0.0 9.019260860871634E-4 0.0 28-29 2.1401635941051335E-4 0.0 0.0 0.0028280733207817835 0.0 30-31 2.1401635941051335E-4 0.0 0.0 0.009691883704733246 1.5286882815036666E-5 32-33 2.1401635941051335E-4 0.0 0.0 0.02436729120716845 3.057376563007333E-5 34-35 2.1401635941051335E-4 0.0 0.0 0.04683900894527235 3.057376563007333E-5 36-37 2.1401635941051335E-4 0.0 0.0 0.08183068370889127 4.586064844511E-5 38-39 2.4459012504058665E-4 0.0 0.0 0.13946223192157953 6.114753126014666E-5 40-41 2.4459012504058665E-4 0.0 0.0 0.20096136148647203 6.114753126014666E-5 42-43 2.7516389067066E-4 0.0 0.0 0.2655178676143719 6.114753126014666E-5 44-45 3.363114219308067E-4 0.0 0.0 0.3488313789563217 7.643441407518333E-5 46-47 3.363114219308067E-4 0.0 0.0 0.4443285359018558 9.172129689022001E-5 48-49 3.363114219308067E-4 0.0 0.0 0.5446257740513113 9.172129689022001E-5 50-51 3.363114219308067E-4 0.0 0.0 0.6611576817503358 9.172129689022001E-5 52-53 3.5159830474584336E-4 0.0 0.0 0.7735009835580403 9.172129689022001E-5 54-55 3.6688518756088003E-4 0.0 0.0 0.8977069064302132 9.172129689022001E-5 56-57 3.6688518756088003E-4 0.0 0.0 1.0452253255953172 9.172129689022001E-5 58-59 3.6688518756088003E-4 0.0 0.0 1.2019617350978882 1.0700817970525666E-4 60-61 3.6688518756088003E-4 0.0 0.0 1.3734958471654144 1.5286882815036668E-4 62-63 3.6688518756088003E-4 0.0 0.0 1.542553484216905 1.5286882815036668E-4 64-65 3.6688518756088003E-4 0.0 0.0 1.7229234145515226 1.5286882815036668E-4 66-67 3.6688518756088003E-4 0.0 0.0 1.935747397102463 1.5286882815036668E-4 68-69 3.6688518756088003E-4 0.0 0.0 2.1676952700550145 1.5286882815036668E-4 70-71 3.6688518756088003E-4 0.0 0.0 2.4193785087217785 1.5286882815036668E-4 72-73 3.6688518756088003E-4 0.0 0.0 2.678277156077239 1.5286882815036668E-4 74-75 3.821720703759167E-4 0.0 0.0 2.943382277855605 1.5286882815036668E-4 76-77 3.9745895319095336E-4 0.0 0.0 3.236875141021494 1.5286882815036668E-4 78-79 3.9745895319095336E-4 0.0 0.0 3.56228701550518 1.6815571096540335E-4 80-81 4.280327188210267E-4 0.0 0.0 3.9121273287272933 1.8344259378044002E-4 82-83 4.280327188210267E-4 0.0 0.0 4.272408582912078 1.8344259378044002E-4 84-85 4.5860648445109997E-4 0.0 0.0 4.640317991621565 1.8344259378044002E-4 86-87 4.891802500811733E-4 0.0 0.0 5.0196772755595145 1.8344259378044002E-4 88-89 4.891802500811733E-4 0.0 0.0 5.424397498087611 1.8344259378044002E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5065 0.0 51.648796 1 GTATCAA 8815 0.0 42.087124 1 ATCAACG 11580 0.0 31.65604 3 TATCAAC 11665 0.0 31.478174 2 TCAACGC 11725 0.0 31.224009 4 CAACGCA 11970 0.0 30.5452 5 AACGCAG 12470 0.0 29.327164 6 ACGCAGA 14510 0.0 25.171247 7 CGCAGAG 14790 0.0 24.630487 8 GTACATG 11210 0.0 23.93043 1 GTAAGGT 1815 0.0 23.576355 4 TAAGGTG 1965 0.0 23.470371 5 TACATGG 11145 0.0 23.259127 2 GTGGTAT 2610 0.0 22.23291 1 ACATGGG 11425 0.0 21.972956 3 GCAGAGT 16280 0.0 21.909443 9 GAGTACT 9545 0.0 20.625021 12-13 TGGTATC 2475 0.0 20.562958 2 GTACTTT 9645 0.0 20.017237 14-15 AGTACTT 9840 0.0 20.00669 12-13 >>END_MODULE