FastQCFastQC Report
Thu 26 May 2016
SRR938428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264256
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71980.2205096659085562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56390.17274993137793115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39490.12097703121323818No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36480.1117559407105325No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34890.10688499921574779No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47450.051.466411
GTATCAA87250.044.8815571
ATCAACG112650.034.2605253
TATCAAC115250.033.693822
TCAACGC114450.033.6801644
CAACGCA116800.032.8397525
AACGCAG122850.031.369556
ACGCAGA141600.027.1092517
CGCAGAG142050.026.9564828
GTACATG119500.024.42721
GCAGAGT156550.023.8209679
TACATGG119750.023.6559222
TGGTATC23450.023.3090292
GTGGTAT26000.022.5643041
ACATGGG121150.022.5586743
GAGTACT84800.022.07072312-13
AGAGTAC143500.020.80492810-11
GTACTTT89900.020.7408314-15
AGTACTT88550.020.49231312-13
CAGAGTA148450.020.14319610-11
Produced by FastQC (version 0.11.5)