##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938428_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3264256 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.951557720963063 31.0 31.0 34.0 28.0 34.0 2 31.124087387753903 31.0 31.0 34.0 27.0 34.0 3 31.17869615618383 31.0 31.0 34.0 27.0 34.0 4 34.844249348090344 37.0 35.0 37.0 32.0 37.0 5 34.82467337120618 37.0 35.0 37.0 32.0 37.0 6 34.795861599090266 37.0 35.0 37.0 32.0 37.0 7 34.70329410438397 37.0 35.0 37.0 31.0 37.0 8 34.64736068494628 37.0 35.0 37.0 30.0 37.0 9 36.11090122833503 39.0 35.0 39.0 31.0 39.0 10-11 35.92991404473179 38.0 35.0 39.0 30.0 39.0 12-13 35.730277741696725 38.0 35.0 39.0 30.0 39.0 14-15 36.8729013594522 39.0 36.0 41.0 30.5 41.0 16-17 36.78531417266293 39.0 36.0 41.0 30.0 41.0 18-19 36.76208774679437 39.0 36.0 41.0 30.0 41.0 20-21 36.67135344164183 39.0 36.0 41.0 30.0 41.0 22-23 36.506146117216296 39.0 36.0 41.0 29.5 41.0 24-25 36.34671208385617 39.0 35.5 41.0 29.0 41.0 26-27 36.115188575896006 39.0 35.0 40.5 28.0 41.0 28-29 35.861616245784646 38.5 35.0 40.0 27.0 41.0 30-31 35.571754788840096 38.0 34.5 40.0 27.0 41.0 32-33 35.264826808926756 38.0 34.0 40.0 25.5 41.0 34-35 34.93666872328641 38.0 34.0 40.0 25.0 41.0 36-37 34.68474929662379 38.0 33.5 40.0 24.5 41.0 38-39 34.415006206621044 38.0 33.0 40.0 24.0 41.0 40-41 34.1079566063446 38.0 33.0 40.0 23.5 41.0 42-43 33.66532863844012 38.0 32.5 40.0 21.5 41.0 44-45 33.17066247255117 37.0 31.5 40.0 18.5 41.0 46-47 32.654871278478154 37.0 31.0 40.0 15.0 41.0 48-49 32.14180336958866 36.5 30.0 39.0 11.0 41.0 50-51 31.400614565769352 35.5 30.0 38.5 9.0 40.0 52-53 31.37204710659948 36.0 30.0 39.0 8.0 40.0 54-55 31.28385380926006 36.0 29.5 39.0 4.5 40.0 56-57 30.80079825234295 35.5 28.5 39.0 2.0 40.0 58-59 30.28370905958356 35.0 28.0 39.0 2.0 40.0 60-61 29.684896803436985 34.5 27.5 38.0 2.0 40.0 62-63 28.905721701974358 34.0 26.0 38.0 2.0 40.0 64-65 28.185105886303035 34.0 25.5 37.5 2.0 40.0 66-67 27.425878515655633 33.0 22.5 37.0 2.0 39.0 68-69 26.514347373490317 32.5 21.0 36.0 2.0 39.0 70-71 25.62091714007725 31.5 17.5 35.5 2.0 38.5 72-73 24.662536118490706 31.0 10.0 35.0 2.0 38.0 74-75 23.67221274924516 30.0 4.5 34.5 2.0 37.0 76-77 22.67247375818563 29.5 2.0 34.0 2.0 36.0 78-79 21.641873370225866 28.5 2.0 34.0 2.0 36.0 80-81 20.629165420849347 27.0 2.0 34.0 2.0 35.0 82-83 19.58540966149714 26.0 2.0 33.0 2.0 35.0 84-85 18.572140787977414 24.5 2.0 32.0 2.0 35.0 86-87 17.554935948651085 21.5 2.0 32.0 2.0 34.0 88-89 16.439464919418086 15.5 2.0 31.0 2.0 34.0 90-91 15.28635269415144 4.5 2.0 31.0 2.0 34.0 92-93 14.13319773326602 2.0 2.0 30.0 2.0 34.0 94-95 12.906904054093797 2.0 2.0 29.0 2.0 34.0 96-97 11.558265344384754 2.0 2.0 28.0 2.0 33.5 98-99 9.975541287202965 2.0 2.0 22.5 2.0 32.5 100-101 7.053512959767861 2.0 2.0 2.0 2.0 28.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45934054308316874 1101 2 0.4451924076953908 1101 3 0.5134093574482712 1101 4 0.40109639895060667 1101 5 0.36974728248718947 1101 6 0.4591020706395881 1101 7 0.35282862817540916 1101 8 0.41532034903914195 1101 9 0.5266692481173081 1101 10-11 0.5271024856373856 1101 12-13 0.5362082352847253 1101 14-15 0.5590589224428868 1101 16-17 0.6474673533149584 1101 18-19 0.553375511664747 1101 20-21 0.7973299256272952 1101 22-23 0.80764885714337 1101 24-25 0.7650201437196174 1101 26-27 0.869626841946733 1101 28-29 0.8498046407140407 1101 30-31 0.9085130104472015 1101 32-33 0.9796606119837676 1101 34-35 0.9803190502497117 1101 36-37 1.0928742882433582 1101 38-39 1.2634727226623639 1101 40-41 1.1953148794513382 1101 42-43 1.3981939128562928 1101 44-45 1.4112069112687564 1101 46-47 1.4571724448878598 1101 48-49 1.3634553117532207 1101 50-51 1.531073442328939 1101 52-53 1.4281817854698602 1101 54-55 1.6755768590467888 1101 56-57 1.8711036576497584 1101 58-59 1.8495111167260987 1101 60-61 1.8381317610683894 1101 62-63 2.054879979159029 1101 64-65 2.0148744828154292 1101 66-67 2.2109716885474597 1101 68-69 2.476042707918783 1101 70-71 2.8317933977762486 1101 72-73 2.896118469690567 1101 74-75 2.9835358896610273 1101 76-77 3.258633578287082 1101 78-79 3.340100051153893 1101 80-81 3.6352473843834154 1101 82-83 3.5276558710692676 1101 84-85 3.8163903290409174 1101 86-87 4.035358406801976 1101 88-89 4.420960342779903 1101 90-91 4.273137920924853 1101 92-93 4.2006227985899525 1101 94-95 4.0259725004699565 1101 96-97 3.726425696595294 1101 98-99 3.407051811003308 1101 100-101 2.612089505070359 1102 1 0.7202960142227859 1102 2 0.5483710661478227 1102 3 0.6023681880992484 1102 4 0.40963604169582624 1102 5 0.5256186419843942 1102 6 0.5403234873672318 1102 7 0.6686554301085437 1102 8 0.6872719876463123 1102 9 0.7291311203764508 1102 10-11 0.7839388789282751 1102 12-13 0.8811303558819006 1102 14-15 0.9216873370515657 1102 16-17 0.9768777834495879 1102 18-19 0.9701725081577948 1102 20-21 0.874426907139366 1102 22-23 0.864208200843315 1102 24-25 0.9695248930007452 1102 26-27 1.0198883502588032 1102 28-29 1.0127000979422007 1102 30-31 0.9794909451734384 1102 32-33 1.0217195296501842 1102 34-35 1.0678158604046857 1102 36-37 1.2787401684813346 1102 38-39 1.4691695106407892 1102 40-41 1.6010071849356606 1102 42-43 1.6402114362625824 1102 44-45 1.6964370047680788 1102 46-47 1.777019532859164 1102 48-49 1.7623724158890326 1102 50-51 1.7608260853293416 1102 52-53 1.8244632125701514 1102 54-55 1.9026502779747894 1102 56-57 2.1279015172195095 1102 58-59 2.3832271397157854 1102 60-61 2.4137946393722096 1102 62-63 2.3443274999662584 1102 64-65 2.4548279848032557 1102 66-67 2.738275484079672 1102 68-69 2.7383641601525035 1102 70-71 2.627055283561514 1102 72-73 2.6881453906063975 1102 74-75 2.586355751985547 1102 76-77 2.673472432624507 1102 78-79 3.088642601622258 1102 80-81 3.366587626524602 1102 82-83 3.8321657024763773 1102 84-85 3.9481262594159894 1102 86-87 3.9589587676820734 1102 88-89 3.864043038504665 1102 90-91 3.7407814895091747 1102 92-93 3.8121776263737086 1102 94-95 3.813713233011727 1102 96-97 3.51132496040462 1102 98-99 3.122604433714532 1102 100-101 2.295563640489651 1103 1 0.5417271339647307 1103 2 0.5034567779545362 1103 3 0.4647318258121018 1103 4 0.4728936059937183 1103 5 0.39685352256933726 1103 6 0.34797267602910864 1103 7 0.48550950721558905 1103 8 0.4990639183895951 1103 9 0.5495308595487671 1103 10-11 0.6987157911493327 1103 12-13 0.7095059081761619 1103 14-15 0.835684173174414 1103 16-17 0.715091351626775 1103 18-19 0.8843223721974525 1103 20-21 0.8231592727768628 1103 22-23 0.9808396858353561 1103 24-25 0.9949749530036698 1103 26-27 0.9655440794013899 1103 28-29 0.971919275969654 1103 30-31 1.0802396355646167 1103 32-33 1.2026681676398923 1103 34-35 1.2000059605592455 1103 36-37 1.2454638983401125 1103 38-39 1.379604654108384 1103 40-41 1.4823316332312615 1103 42-43 1.4572871350868937 1103 44-45 1.7484299638665775 1103 46-47 1.9816106272337706 1103 48-49 1.8009289816095944 1103 50-51 1.914944367679695 1103 52-53 2.0570127499473045 1103 54-55 2.0153537927363523 1103 56-57 2.2060407966934896 1103 58-59 2.293177369728596 1103 60-61 2.5055235779835208 1103 62-63 2.7371777046155294 1103 64-65 2.834547851908237 1103 66-67 3.117104012326905 1103 68-69 3.2908253361565833 1103 70-71 3.1769312058436903 1103 72-73 3.4452925126065104 1103 74-75 3.5350848829639325 1103 76-77 3.5032787031824313 1103 78-79 3.593745419067421 1103 80-81 3.7301078927104108 1103 82-83 3.8941031928794274 1103 84-85 4.2303400867464624 1103 86-87 4.168846330931547 1103 88-89 4.432440761532984 1103 90-91 4.522132709365721 1103 92-93 4.416973160717509 1103 94-95 4.373994340109954 1103 96-97 4.142539477859126 1103 98-99 3.725844810556124 1103 100-101 2.850593088255331 1104 1 0.542749133681145 1104 2 0.47901985944183423 1104 3 0.43854703691994246 1104 4 0.4379227462559925 1104 5 0.46821008085532156 1104 6 0.5397394157736599 1104 7 0.5306834580389008 1104 8 0.44638294154546543 1104 9 0.6235242207316816 1104 10-11 0.6150635146458683 1104 12-13 0.6440691132778014 1104 14-15 0.7654845905545926 1104 16-17 0.7837048532210389 1104 18-19 0.7293616197497101 1104 20-21 0.7755737516515353 1104 22-23 0.8228775590870328 1104 24-25 0.9669510372650265 1104 26-27 0.88451106346605 1104 28-29 0.9397756397448376 1104 30-31 0.8595678643529396 1104 32-33 0.9961197889873503 1104 34-35 1.0491067571445853 1104 36-37 1.1511976039707434 1104 38-39 1.1040440267292553 1104 40-41 1.211208124329211 1104 42-43 1.2850944530556916 1104 44-45 1.216668259588431 1104 46-47 1.3426088493687374 1104 48-49 1.4555771533898962 1104 50-51 1.5387464902105847 1104 52-53 1.619067329313168 1104 54-55 1.9260633390353696 1104 56-57 2.2816505062361507 1104 58-59 2.33128258007293 1104 60-61 2.2120755923518693 1104 62-63 2.234950828677057 1104 64-65 2.295615698001118 1104 66-67 2.5837475921981294 1104 68-69 2.932609436444171 1104 70-71 3.003246789612401 1104 72-73 3.29066951815825 1104 74-75 3.1856739598193293 1104 76-77 3.455252260621286 1104 78-79 3.631276731669903 1104 80-81 3.5772554009725717 1104 82-83 3.7734156063039457 1104 84-85 3.776484319228672 1104 86-87 3.8682157160229345 1104 88-89 4.2124559588251 1104 90-91 4.08387598056467 1104 92-93 3.986666833481271 1104 94-95 3.9673360719468267 1104 96-97 3.919116287809352 1104 98-99 3.5693691462199517 1104 100-101 2.2864734223048986 1105 1 0.5165023610098736 1105 2 0.47300169325000496 1105 3 0.40402775731068274 1105 4 0.39089680986930375 1105 5 0.4299592271181183 1105 6 0.42386886746611196 1105 7 0.42845020807273215 1105 8 0.4614696158634004 1105 9 0.26458410272876876 1105 10-11 0.5496266894409843 1105 12-13 0.6633563240045675 1105 14-15 0.6885719204442609 1105 16-17 0.7323965657747991 1105 18-19 0.6845591788434362 1105 20-21 0.6209877372992594 1105 22-23 0.6660589538554618 1105 24-25 0.7055689584522966 1105 26-27 0.591777276563775 1105 28-29 0.6968420616412843 1105 30-31 0.8142403509031766 1105 32-33 0.884659278836537 1105 34-35 0.8013549903523653 1105 36-37 0.9719924978929058 1105 38-39 1.125118417425412 1105 40-41 1.0054653685727502 1105 42-43 1.189003409431173 1105 44-45 1.2802570051967095 1105 46-47 1.4045279521669514 1105 48-49 1.3967535180073085 1105 50-51 1.2214960776222057 1105 52-53 1.2981508118930591 1105 54-55 1.6109051318219763 1105 56-57 1.7801919469824874 1105 58-59 1.8836794593405202 1105 60-61 1.9535229734921415 1105 62-63 1.8922071994107874 1105 64-65 2.048214257852358 1105 66-67 2.3861193382862282 1105 68-69 2.428917978737335 1105 70-71 2.4692132570297822 1105 72-73 2.4915475976318255 1105 74-75 2.7795931803744054 1105 76-77 2.8999036827550064 1105 78-79 3.1609827437724967 1105 80-81 3.335449915963725 1105 82-83 3.4290159114172276 1105 84-85 3.374495063223513 1105 86-87 3.5898752440464285 1105 88-89 3.631119541551051 1105 90-91 3.629843467369753 1105 92-93 3.583926657830224 1105 94-95 3.5906010477187937 1105 96-97 3.4548639735356392 1105 98-99 2.97998242216293 1105 100-101 2.1189916262843775 1106 1 0.3380783334376609 1106 2 0.34153277686939276 1106 3 0.20982929509861847 1106 4 0.3224911980049825 1106 5 0.27720981188970484 1106 6 -0.017178662962479052 1106 7 0.31091094632473926 1106 8 -0.00761650636721356 1106 9 0.3638386273793799 1106 10-11 0.43069059382085584 1106 12-13 0.6825549076465052 1106 14-15 0.6640003250430553 1106 16-17 0.16880716632532966 1106 18-19 0.6287642329630003 1106 20-21 0.5907920738723362 1106 22-23 0.5731383849530332 1106 24-25 0.6558921267173261 1106 26-27 0.6423767151414168 1106 28-29 0.732702905780144 1106 30-31 0.5980476423855308 1106 32-33 0.7627241688800481 1106 34-35 0.7241778914716761 1106 36-37 0.8260512654421248 1106 38-39 0.8729266515505358 1106 40-41 0.9884170044413239 1106 42-43 0.6564653637166415 1106 44-45 1.0240584437590954 1106 46-47 1.2906420383100041 1106 48-49 1.1901020751775278 1106 50-51 1.067773011635822 1106 52-53 1.2625169548847168 1106 54-55 1.2258025131693735 1106 56-57 1.666772107006242 1106 58-59 1.6890712580361509 1106 60-61 2.07870386063799 1106 62-63 2.1603172943273172 1106 64-65 2.0589799269899487 1106 66-67 2.2120332195250327 1106 68-69 2.397415816591675 1106 70-71 2.2931717269717247 1106 72-73 2.379042948602322 1106 74-75 2.474300607233719 1106 76-77 2.8524233439864695 1106 78-79 3.270947036450412 1106 80-81 3.00616106482218 1106 82-83 3.1670777501014555 1106 84-85 3.3122919592677427 1106 86-87 3.305149118721083 1106 88-89 3.1378997520896377 1106 90-91 3.1855810810020913 1106 92-93 3.342408187652362 1106 94-95 3.2945316807292624 1106 96-97 3.1918764412613125 1106 98-99 2.7400768442989705 1106 100-101 1.9735285673339904 1107 1 0.26455774028429246 1107 2 0.18409249752291856 1107 3 0.17392448861626164 1107 4 0.27132595418733274 1107 5 0.2191490923588546 1107 6 0.24893751885014836 1107 7 0.3351899400533469 1107 8 0.33783690592660776 1107 9 0.44217214726025844 1107 10-11 0.3088609269947753 1107 12-13 0.2983322512772659 1107 14-15 0.37285514356621263 1107 16-17 0.5905719654814519 1107 18-19 0.54388408128289 1107 20-21 0.5343831056837871 1107 22-23 0.6083965498000126 1107 24-25 0.5342858081822968 1107 26-27 0.6192574959955408 1107 28-29 0.5819828322464957 1107 30-31 0.6316316277873355 1107 32-33 0.7661141617912435 1107 34-35 0.843598763089652 1107 36-37 0.8487435450870748 1107 38-39 0.9628892487672474 1107 40-41 0.9542389922919909 1107 42-43 0.9633341716099793 1107 44-45 0.8142925948490429 1107 46-47 0.8500199955126035 1107 48-49 0.7250057996841051 1107 50-51 1.114553412950471 1107 52-53 1.282233249982582 1107 54-55 1.324798293803024 1107 56-57 1.4086230317733133 1107 58-59 1.3284767140431555 1107 60-61 1.5152004044206997 1107 62-63 1.766236320820461 1107 64-65 1.8162434525350903 1107 66-67 1.9649213995332424 1107 68-69 2.1398038711042595 1107 70-71 2.2009145101254646 1107 72-73 2.2785955312683264 1107 74-75 2.3666692862396594 1107 76-77 2.480391866631649 1107 78-79 2.799448279535433 1107 80-81 2.7554667687678283 1107 82-83 2.9518728954559137 1107 84-85 2.8996230489428463 1107 86-87 2.903985782776086 1107 88-89 3.1628000339460236 1107 90-91 3.035207392346557 1107 92-93 2.824345636563775 1107 94-95 2.8091538710688138 1107 96-97 2.788905332388774 1107 98-99 2.481230717195647 1107 100-101 1.7644942815906335 1108 1 0.2916087082801617 1108 2 0.22924191034431374 1108 3 0.21169682640747922 1108 4 0.2333116430271147 1108 5 0.17433716439876434 1108 6 0.21834893951806578 1108 7 0.08401593097484295 1108 8 0.24739464921776033 1108 9 0.2879774438885647 1108 10-11 0.31466107943636246 1108 12-13 0.2465653910619423 1108 14-15 0.3863393839651934 1108 16-17 0.48834993075082167 1108 18-19 0.43031460957366363 1108 20-21 0.43941646303151316 1108 22-23 0.46565368254665174 1108 24-25 0.6452196214212975 1108 26-27 0.49105694971239444 1108 28-29 0.5454761855558701 1108 30-31 0.5859357679975545 1108 32-33 0.5503287988864827 1108 34-35 0.6835002453608396 1108 36-37 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2.3557023374489034 2201 82-83 2.538202879424272 2201 84-85 2.5972716995604657 2201 86-87 2.865777797612612 2201 88-89 2.7083135538786145 2201 90-91 2.7906887858317404 2201 92-93 2.7477071098407944 2201 94-95 2.6497439887226104 2201 96-97 2.4183215608558637 2201 98-99 2.283621987763164 2201 100-101 1.4152933580131721 2202 1 0.4170233258216456 2202 2 0.4627657786820727 2202 3 0.5387704967059079 2202 4 0.4639243872887988 2202 5 0.4684152099557153 2202 6 0.5694971015682526 2202 7 0.5412061444445087 2202 8 0.5405913177958865 2202 9 0.5485848865720087 2202 10-11 0.5410202018545505 2202 12-13 0.5116163802598734 2202 14-15 0.720831476956711 2202 16-17 0.7807016759022076 2202 18-19 0.7356699942543443 2202 20-21 0.6351807766685553 2202 22-23 0.7215817541953626 2202 24-25 0.6710518734934467 2202 26-27 0.7162004543635518 2202 28-29 0.8551939285316905 2202 30-31 0.811498696566062 2202 32-33 0.9352798071434663 2202 34-35 0.8907623672072518 2202 36-37 1.026763672998868 2202 38-39 1.1100796231748475 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0.4167207071336385 2203 4 0.30762068215715743 2203 5 0.33197342601987856 2203 6 0.4149080369864748 2203 7 0.3127237659543738 2203 8 0.3961537918869169 2203 9 0.343798359405767 2203 10-11 0.4148959445782907 2203 12-13 0.38482262673070267 2203 14-15 0.5247671664209932 2203 16-17 0.6648456436121677 2203 18-19 0.7680546257797829 2203 20-21 0.643369289890444 2203 22-23 0.7578730742007522 2203 24-25 0.7608920610103951 2203 26-27 0.8287577952803602 2203 28-29 0.9335634590740014 2203 30-31 0.7933224149358011 2203 32-33 0.9165926817039676 2203 34-35 1.0439560579578213 2203 36-37 1.1598282070127581 2203 38-39 1.2742532571858405 2203 40-41 1.2969097462462926 2203 42-43 1.19763032338642 2203 44-45 1.3815523883467122 2203 46-47 1.49119558461679 2203 48-49 1.6268384050235554 2203 50-51 1.5162360400751709 2203 52-53 1.4787793855984859 2203 54-55 1.4877471685676475 2203 56-57 1.3863809225246975 2203 58-59 1.6125012428059797 2203 60-61 1.713912556224006 2203 62-63 1.9342654819918472 2203 64-65 2.0714449639864085 2203 66-67 2.2438441924440937 2203 68-69 2.315186791973016 2203 70-71 2.436635979634932 2203 72-73 2.543896709182871 2203 74-75 2.589941346637268 2203 76-77 2.6479626560971568 2203 78-79 2.5643865798494936 2203 80-81 2.5283665490684264 2203 82-83 2.621219413551941 2203 84-85 2.68965455570131 2203 86-87 2.6187905172688914 2203 88-89 2.618413324374867 2203 90-91 2.3686269292232325 2203 92-93 2.079395448807672 2203 94-95 2.1824534673491183 2203 96-97 2.182698047636684 2203 98-99 2.142036830161606 2203 100-101 1.3024222735783608 2204 1 0.22482323180735975 2204 2 0.3977762606860722 2204 3 0.32497251305161257 2204 4 0.3844393467440881 2204 5 0.334804178497933 2204 6 0.37589217515250795 2204 7 0.47821346292672473 2204 8 0.5016151653010112 2204 9 0.4404830921437437 2204 10-11 0.5139898759411636 2204 12-13 0.6392146600843844 2204 14-15 0.7095337434745943 2204 16-17 0.7400048433762905 2204 18-19 0.7241072884929878 2204 20-21 0.7100877925055045 2204 22-23 0.6884450828223763 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2.002675949130566 2204 92-93 1.8504195558991423 2204 94-95 1.6691230661729701 2204 96-97 1.918828415328182 2204 98-99 1.9431184316122483 2204 100-101 1.0983412643537127 2205 1 0.22972660648287402 2205 2 0.3729709535040726 2205 3 0.4377501305883875 2205 4 0.386458383375178 2205 5 0.29067380641832585 2205 6 0.4021396513567197 2205 7 0.42312260627832643 2205 8 0.4721949172479398 2205 9 0.5174949179297315 2205 10-11 0.5157204787678182 2205 12-13 0.4824508873702271 2205 14-15 0.5471455115738166 2205 16-17 0.6259292737195281 2205 18-19 0.6703143839645733 2205 20-21 0.6616309133592679 2205 22-23 0.6221053078007444 2205 24-25 0.6034807468104546 2205 26-27 0.6295218495086132 2205 28-29 0.7418855675673015 2205 30-31 0.6519871503842865 2205 32-33 0.7402693755943588 2205 34-35 0.7743242051444597 2205 36-37 0.7159248267677256 2205 38-39 0.662288958843682 2205 40-41 0.9586450359112959 2205 42-43 1.188025553646547 2205 44-45 1.1955271411906736 2205 46-47 1.190308114812467 2205 48-49 1.192943795934056 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9 0.2106036754128766 2206 10-11 -0.05548650250956655 2206 12-13 0.12328460972515387 2206 14-15 0.16084145816115836 2206 16-17 0.24712898394030702 2206 18-19 -0.04655955833753467 2206 20-21 -0.07466418307504341 2206 22-23 0.05331031580222856 2206 24-25 0.0786084595353671 2206 26-27 0.21112552817326247 2206 28-29 0.2729095590642743 2206 30-31 0.3545556314044944 2206 32-33 0.44854935254954853 2206 34-35 0.4376241220692165 2206 36-37 0.4739028700869383 2206 38-39 0.6320165728549725 2206 40-41 0.6823848322436419 2206 42-43 0.20438514032498034 2206 44-45 0.502216612924947 2206 46-47 0.899742408337751 2206 48-49 1.116039686429204 2206 50-51 1.0015211711970196 2206 52-53 0.8804516438973593 2206 54-55 0.7232795889426988 2206 56-57 0.4688540576705691 2206 58-59 0.4191465296859427 2206 60-61 0.7669607974671209 2206 62-63 0.8329014865320943 2206 64-65 0.8801795101982037 2206 66-67 1.1787255315109775 2206 68-69 0.9223244923674514 2206 70-71 1.0607494491364307 2206 72-73 1.3134812514437648 2206 74-75 1.4728325105554632 2206 76-77 1.5957555184304582 2206 78-79 1.4289084190581534 2206 80-81 1.5044203483596874 2206 82-83 1.1480511609935853 2206 84-85 1.002134243106802 2206 86-87 0.8851514960608959 2206 88-89 0.8614451672783368 2206 90-91 1.3205924554754844 2206 92-93 1.283919161534639 2206 94-95 1.2055476131604 2206 96-97 0.980611509286927 2206 98-99 0.792654981689811 2206 100-101 0.541046251883289 2207 1 0.13930199683743538 2207 2 0.2751174803245391 2207 3 0.22638126944498893 2207 4 0.15024774265640417 2207 5 0.30607649795040714 2207 6 0.29810316917210145 2207 7 0.2338049378515521 2207 8 0.2364230875617821 2207 9 0.25287146683353967 2207 10-11 0.2611931083580288 2207 12-13 0.38084104325094614 2207 14-15 0.5005113545654609 2207 16-17 0.5248924215073814 2207 18-19 0.4200636668577573 2207 20-21 0.4673171389928399 2207 22-23 0.4021665765259925 2207 24-25 0.4897165387937932 2207 26-27 0.5193309569181892 2207 28-29 0.6932174830737381 2207 30-31 0.755305526228625 2207 32-33 0.6540945749176146 2207 34-35 0.7194709086153352 2207 36-37 0.913573418113323 2207 38-39 0.9735455470267098 2207 40-41 1.1089649444936853 2207 42-43 1.3330772004322284 2207 44-45 1.3128308848329908 2207 46-47 1.1387682985044094 2207 48-49 1.3071832057829837 2207 50-51 1.248123634084088 2207 52-53 1.223166203700174 2207 54-55 1.3468804036331967 2207 56-57 1.3972576935588172 2207 58-59 1.4088677209651195 2207 60-61 1.4979870085576472 2207 62-63 1.5883153866897466 2207 64-65 1.6945438671225617 2207 66-67 1.7706006188181576 2207 68-69 1.8403530016475678 2207 70-71 1.8650036860034938 2207 72-73 1.7942979049490226 2207 74-75 1.8440798495956479 2207 76-77 1.990601717294929 2207 78-79 2.122414964398221 2207 80-81 1.9807192653230814 2207 82-83 1.7388989253800808 2207 84-85 2.006552118566347 2207 86-87 2.034456642938551 2207 88-89 2.1423444138810446 2207 90-91 1.7856289809288626 2207 92-93 1.588184801787941 2207 94-95 1.523963552487869 2207 96-97 1.5404192970666504 2207 98-99 1.4643324727493532 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-0.42470809731332704 2316 1 -1.0878181173873855 2316 2 -0.7418604969358888 2316 3 -0.8694686289688605 2316 4 -0.7619727908778984 2316 5 -0.7165144381728581 2316 6 -0.5785045811526501 2316 7 -0.8425345648154519 2316 8 -0.639997048326947 2316 9 -0.8943013935630901 2316 10-11 -0.8551568665904767 2316 12-13 -0.9585267889449582 2316 14-15 -1.1134651681184877 2316 16-17 -1.1708014800615345 2316 18-19 -1.0818159069237296 2316 20-21 -0.9956727559246801 2316 22-23 -1.2723050207562352 2316 24-25 -1.0633375281234194 2316 26-27 -1.3639639557444951 2316 28-29 -1.196559997673404 2316 30-31 -1.1021636696222785 2316 32-33 -1.3983956449584696 2316 34-35 -1.2431926475941353 2316 36-37 -1.4879441007924115 2316 38-39 -1.6137282052703696 2316 40-41 -1.4806219942627052 2316 42-43 -1.1968271856302692 2316 44-45 -1.3009131063609232 2316 46-47 -0.9556878747466051 2316 48-49 -0.8856843508045529 2316 50-51 -0.8918722840681745 2316 52-53 -0.76976789202417 2316 54-55 -0.8702827623750515 2316 56-57 -0.810618976088378 2316 58-59 -0.723863721056329 2316 60-61 -0.8021296754094749 2316 62-63 -0.6860380628479952 2316 64-65 -0.7387747169112195 2316 66-67 -0.7296429537354356 2316 68-69 -0.6399000980055582 2316 70-71 -0.7575963673616464 2316 72-73 -0.4704364780102672 2316 74-75 -0.32943995782143887 2316 76-77 -0.2890549558151463 2316 78-79 -0.4530933218363309 2316 80-81 -0.42974352252873516 2316 82-83 -0.4189704672025023 2316 84-85 -0.07409314795405209 2316 86-87 0.015005341975051323 2316 88-89 -0.2085867576343503 2316 90-91 -0.2085670163510187 2316 92-93 -0.3283578480840408 2316 94-95 -0.3497862770866238 2316 96-97 -0.16840686222411172 2316 98-99 -0.21809964771199652 2316 100-101 -0.05719913795600995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12314.0 3 5024.0 4 5561.0 5 6417.0 6 7700.0 7 9667.0 8 12621.0 9 16056.0 10 20178.0 11 25300.0 12 30459.0 13 35881.0 14 40768.0 15 45547.0 16 51609.0 17 58409.0 18 59444.0 19 61748.0 20 67226.0 21 74056.0 22 81463.0 23 90211.0 24 98300.0 25 108212.0 26 118690.0 27 129756.0 28 141655.0 29 154584.0 30 167892.0 31 184229.0 32 202306.0 33 222780.0 34 242750.0 35 255143.0 36 236455.0 37 151424.0 38 32080.0 39 341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58768225810916 18.059091454282086 14.435549596041298 23.917676691567458 2 15.721909565755665 19.369892944314802 39.96417687247855 24.94402061745098 3 19.664370665117204 23.10101793896779 28.74375127181079 28.490860124104216 4 13.113573860569721 15.952958686065033 35.89874673166707 35.03472072169818 5 14.412099564272957 36.796219897772446 33.56204864668343 15.229631891271175 6 33.691637182639944 34.930502703294195 17.019243825519133 14.358616288546733 7 30.16862623952024 31.431714065405725 19.716018236225068 18.68364145884897 8 27.061355749460063 34.630506891285606 19.1459776903229 19.162159668931427 9 26.618994527092088 16.736623125717518 19.3128861881555 37.33149615903489 10-11 25.55158917566483 26.006142849130903 26.887943877086627 21.554324098117643 12-13 26.42585076519492 23.14964191412037 27.162967878529816 23.26153944215489 14-15 22.977035926267487 24.86583899637687 25.23015937829274 26.926965699062904 16-17 23.030294023377486 27.951356863619903 25.97454191565941 23.043807197343206 18-19 23.026566481013404 26.51211955598273 28.022534570776607 22.43877939222726 20-21 23.419835505004784 25.838747955151547 28.192675749706257 22.548740790137412 22-23 23.128167324041467 25.67646065994495 28.107779537008533 23.087592479005046 24-25 22.99300256938701 25.818638698397915 28.210956067267155 22.977402664947917 26-27 22.59762982027437 26.26315066051916 27.936226814483533 23.202992704722934 28-29 22.73457252921206 26.570772022066308 27.944637789483185 22.75001765923845 30-31 22.757537826080092 26.08144289124976 27.87030086334619 23.290718419323948 32-33 22.49693364805098 26.122913022859933 27.866715391209816 23.51343793787927 34-35 23.31050084181533 26.193572784389247 27.55063609613427 22.945290277661158 36-37 23.332628997952423 25.79634206305707 27.604578864391737 23.266450074598772 38-39 22.74546175421438 26.161042079157866 27.616197659960797 23.477298506666962 40-41 23.23544672786437 25.926395159497684 27.761790076486147 23.0763680361518 42-43 23.03529225404491 25.95575687745566 27.51221251920522 23.496738349294212 44-45 22.943819832273864 26.210533314215052 27.656169383055566 23.189477470455515 46-47 23.257261928891857 26.516383779339154 27.020738125343087 23.2056161664259 48-49 23.16328387788256 26.42979791511295 27.045912617605044 23.361005589399443 50-51 23.34015854131937 26.609548571164204 26.95462855416604 23.09566433335039 52-53 23.51505085682545 26.336372273371637 26.976023348275273 23.17255352152764 54-55 22.975948514674336 26.46548803032355 27.27210617163368 23.286457283368435 56-57 23.08402274226076 26.648963263267788 27.071470533943735 23.195543460527716 58-59 23.14104010488638 26.129701463204213 27.1301752533276 23.599083178581807 60-61 22.79617225261515 26.4167970150813 27.41310263131974 23.373928100983807 62-63 22.91100847623671 26.475528659901016 27.426816992425756 23.186645871436518 64-65 22.66309950720504 26.382054726741593 27.287008258183505 23.66783750786986 66-67 22.651007431044707 26.49144768122975 27.187740491841044 23.669804395884494 68-69 23.0669825741785 26.131011749106282 27.33856601743455 23.463439659280667 70-71 22.954709619006838 26.21746766969818 27.030729010677064 23.797093700617918 72-73 22.999566309526926 26.261221989929982 26.970920984843506 23.76829071569958 74-75 22.93867616550704 26.627861624217143 26.86889694552761 23.564565264748207 76-77 23.15277614169008 26.242967524888915 26.888974093102334 23.715282240318672 78-79 23.240589298322124 26.316125678944147 26.881968000144607 23.561317022589115 80-81 23.29859670687835 26.50355679443021 26.58415071077676 23.613695787914683 82-83 23.24074750680679 26.50791484193525 26.555711353800966 23.695626297456997 84-85 23.32220589258037 26.31647397382907 26.59372555723754 23.767594576353023 86-87 23.425265569733604 26.368900558382297 26.37328146906947 23.832552402814628 88-89 23.305318249443072 26.37840325511998 26.274547413585093 24.041731081851854 90-91 23.313919762894177 26.39314533242343 26.28630736546123 24.006627539221164 92-93 23.441051690166002 25.944260323158137 26.492018171172642 24.12266981550322 94-95 23.25109154048084 25.900465358278684 26.472196934157026 24.376246167083448 96-97 23.46747330616277 25.68938843558655 26.346412116016115 24.496726142234557 98-99 23.30319026136064 25.781908856284662 25.94801771603152 24.96688316632318 100-101 24.285321881408407 24.72581159935297 26.291397421844554 24.69746909739407 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 337.0 1 260.5 2 264.0 3 367.5 4 318.5 5 283.0 6 477.5 7 1051.5 8 1711.5 9 1951.0 10 1734.5 11 1487.0 12 1490.5 13 1580.0 14 1785.0 15 2085.0 16 2343.0 17 2547.0 18 2813.0 19 3158.5 20 3594.5 21 4244.5 22 5085.0 23 6144.0 24 7514.0 25 9414.5 26 12067.5 27 15512.0 28 19080.5 29 22870.0 30 28141.0 31 35146.5 32 43266.5 33 48293.5 34 51251.5 35 56320.0 36 63173.5 37 70406.5 38 75915.0 39 80919.5 40 87171.5 41 95468.5 42 102934.0 43 108294.0 44 113766.5 45 119940.0 46 123954.5 47 126952.5 48 128895.0 49 127990.5 50 126432.0 51 123733.0 52 121634.0 53 121445.0 54 121103.5 55 115249.5 56 106007.0 57 97374.5 58 90190.0 59 80676.0 60 67861.0 61 57523.0 62 48346.5 63 38543.5 64 29726.0 65 23154.5 66 17803.5 67 13530.5 68 10243.0 69 7708.5 70 5990.5 71 4568.0 72 3510.0 73 2825.5 74 2233.5 75 1716.5 76 1201.0 77 791.0 78 569.0 79 372.0 80 210.0 81 131.0 82 86.0 83 52.0 84 28.5 85 19.0 86 15.5 87 13.0 88 10.5 89 7.0 90 4.5 91 3.0 92 2.0 93 1.5 94 2.0 95 2.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38863373460905026 2 0.030665487020625836 3 0.036516743784801194 4 0.04616672221786527 5 0.06500715630146654 6 0.05376416555564269 7 0.03982530781899459 8 0.04187784291428123 9 0.0390288016626147 10-11 0.03905943651478316 12-13 0.030711439298878523 14-15 0.028168746568896556 16-17 0.02371137557838601 18-19 0.02801557230805427 20-21 0.03608785585444279 22-23 0.03524539741981021 24-25 0.043363633244451416 26-27 0.03843142204532978 28-29 0.03343794114187122 30-31 0.036654600619559254 32-33 0.03104842267273155 34-35 0.03277929182024939 36-37 0.03469397008077798 38-39 0.016849168692651555 40-41 0.014168619127911538 42-43 0.015148934397302173 44-45 0.011151086189318484 46-47 0.020755112344129873 48-49 0.017706944553368364 50-51 0.011488069563171516 52-53 0.008853472276684182 54-55 0.007903791859462004 56-57 0.008838154850599952 58-59 0.00467181495568975 60-61 0.0075821259116932 62-63 0.011227673319739628 64-65 0.0067703023292290795 66-67 0.003431103442867226 68-69 0.006754984903144851 70-71 0.01481195102344914 72-73 0.01248370225864638 74-75 0.01283600305858364 76-77 0.006203557564112618 78-79 0.008347997215904634 80-81 0.011089816484981571 82-83 0.012851320484667871 84-85 0.007750617598619716 86-87 0.002894993529919222 88-89 0.018304324170653283 90-91 0.027663271508117013 92-93 0.012407115128225236 94-95 0.022945504274174574 96-97 0.02067852521370873 98-99 0.013249573562857815 100-101 0.12665979629048701 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3264256.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.907283259413866 #Duplication Level Percentage of deduplicated Percentage of total 1 77.25304064577607 41.645015447428634 2 12.491451446626009 13.46760422908967 3 4.192066966039703 6.779488241422019 4 1.9189350249725023 4.137782957904124 5 1.060192998391855 2.857606213697852 6 0.6757099129205125 2.1855411406199967 7 0.43248494824176653 1.63198620272113 8 0.32144332338600745 1.3862509028493504 9 0.23554125574851834 1.142765027362208 >10 1.27384916272559 12.437384388452525 >50 0.09056958897453982 3.340303066676585 >100 0.04888011351117911 4.847324482984094 >500 0.0030889125997369 1.1340588849406914 >1k 0.0026312959157977025 2.6110459257613896 >5k 1.1440417025207402E-4 0.3958428880897427 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7198 0.2205096659085562 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5639 0.17274993137793115 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3949 0.12097703121323818 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3648 0.1117559407105325 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3489 0.10688499921574779 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.063485216845737E-5 0.0 3 0.0 0.0 0.0 3.063485216845737E-5 0.0 4 0.0 0.0 0.0 3.063485216845737E-5 0.0 5 0.0 0.0 0.0 3.063485216845737E-5 0.0 6 0.0 0.0 0.0 3.063485216845737E-5 0.0 7 0.0 0.0 0.0 3.063485216845737E-5 0.0 8 0.0 0.0 0.0 3.063485216845737E-5 0.0 9 0.0 0.0 0.0 6.126970433691475E-5 0.0 10-11 0.0 0.0 0.0 9.190455650537213E-5 0.0 12-13 0.0 0.0 0.0 1.5317426084228688E-4 0.0 14-15 0.0 0.0 0.0 1.5317426084228688E-4 0.0 16-17 0.0 0.0 0.0 1.5317426084228688E-4 3.063485216845737E-5 18-19 0.0 0.0 0.0 1.5317426084228688E-4 3.063485216845737E-5 20-21 0.0 0.0 0.0 2.757136695161164E-4 4.595227825268606E-5 22-23 0.0 0.0 0.0 5.36109912948004E-4 6.126970433691475E-5 24-25 0.0 0.0 0.0 7.965061563798918E-4 6.126970433691475E-5 26-27 0.0 0.0 0.0 0.001087537251980237 6.126970433691475E-5 28-29 4.595227825268606E-5 0.0 0.0 0.0024967404517292763 6.126970433691475E-5 30-31 6.126970433691475E-5 0.0 0.0 0.008899424554936867 6.126970433691475E-5 32-33 6.126970433691475E-5 0.0 0.0 0.02332843992628029 6.126970433691475E-5 34-35 6.126970433691475E-5 0.0 0.0 0.0451710895223904 6.126970433691475E-5 36-37 9.190455650537213E-5 0.0 0.0 0.08161124617677046 6.126970433691475E-5 38-39 1.5317426084228688E-4 0.0 0.0 0.13843889694925887 6.126970433691475E-5 40-41 1.5317426084228688E-4 0.0 0.0 0.19875892086895144 6.126970433691475E-5 42-43 1.5317426084228688E-4 0.0 0.0 0.2626632224923535 6.126970433691475E-5 44-45 1.5317426084228688E-4 0.0 0.0 0.3415326494000471 7.658713042114344E-5 46-47 1.5317426084228688E-4 1.5317426084228686E-5 0.0 0.43731251470472904 9.190455650537213E-5 48-49 1.8380911301074426E-4 3.063485216845737E-5 0.0 0.543538864598855 9.190455650537213E-5 50-51 1.8380911301074426E-4 3.063485216845737E-5 0.0 0.6597062240216454 9.190455650537213E-5 52-53 1.8380911301074426E-4 3.063485216845737E-5 0.0 0.768796932593522 9.190455650537213E-5 54-55 1.8380911301074426E-4 3.063485216845737E-5 0.0 0.8911525321543409 9.190455650537213E-5 56-57 1.8380911301074426E-4 3.063485216845737E-5 0.0 1.0384295839541997 9.190455650537213E-5 58-59 1.8380911301074426E-4 3.063485216845737E-5 0.0 1.196382881734766 9.190455650537213E-5 60-61 1.8380911301074426E-4 3.063485216845737E-5 0.0 1.3668351992000627 9.190455650537213E-5 62-63 1.8380911301074426E-4 3.063485216845737E-5 0.0 1.5330599070661126 1.225394086738295E-4 64-65 1.8380911301074426E-4 3.063485216845737E-5 0.0 1.712197205121167 1.225394086738295E-4 66-67 1.8380911301074426E-4 3.063485216845737E-5 0.0 1.924527365500745 1.225394086738295E-4 68-69 1.8380911301074426E-4 3.063485216845737E-5 0.0 2.1582559701199906 1.5317426084228688E-4 70-71 1.8380911301074426E-4 3.063485216845737E-5 0.0 2.4067505734844326 2.297613912634303E-4 72-73 1.8380911301074426E-4 3.063485216845737E-5 0.0 2.6599935789349853 2.603962434318877E-4 74-75 1.8380911301074426E-4 3.063485216845737E-5 0.0 2.918812127480198 3.369833738530311E-4 76-77 1.8380911301074426E-4 3.063485216845737E-5 0.0 3.196563014665516 3.369833738530311E-4 78-79 1.8380911301074426E-4 3.063485216845737E-5 0.0 3.505132563132303 3.369833738530311E-4 80-81 1.8380911301074426E-4 3.063485216845737E-5 0.0 3.830490010587405 3.523007999372598E-4 82-83 1.8380911301074426E-4 3.063485216845737E-5 0.0 4.162755617206494 3.8293565210571724E-4 84-85 1.8380911301074426E-4 3.063485216845737E-5 0.0 4.507321116971218 3.982530781899459E-4 86-87 1.8380911301074426E-4 3.063485216845737E-5 0.0 4.857661286369696 3.982530781899459E-4 88-89 1.8380911301074426E-4 3.063485216845737E-5 0.0 5.2249731638695005 3.982530781899459E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4745 0.0 51.46641 1 GTATCAA 8725 0.0 44.881557 1 ATCAACG 11265 0.0 34.260525 3 TATCAAC 11525 0.0 33.69382 2 TCAACGC 11445 0.0 33.680164 4 CAACGCA 11680 0.0 32.839752 5 AACGCAG 12285 0.0 31.36955 6 ACGCAGA 14160 0.0 27.109251 7 CGCAGAG 14205 0.0 26.956482 8 GTACATG 11950 0.0 24.4272 1 GCAGAGT 15655 0.0 23.820967 9 TACATGG 11975 0.0 23.655922 2 TGGTATC 2345 0.0 23.309029 2 GTGGTAT 2600 0.0 22.564304 1 ACATGGG 12115 0.0 22.558674 3 GAGTACT 8480 0.0 22.070723 12-13 AGAGTAC 14350 0.0 20.804928 10-11 GTACTTT 8990 0.0 20.74083 14-15 AGTACTT 8855 0.0 20.492313 12-13 CAGAGTA 14845 0.0 20.143196 10-11 >>END_MODULE