FastQCFastQC Report
Thu 26 May 2016
SRR938428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264256
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76130.23322312955846602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62910.19272385499176534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45180.13840826209709042No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35690.10933578738922438No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35540.10887626460669751No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33780.10348453062504902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49100.050.4181631
GTATCAA86850.040.977251
ATCAACG112300.030.9192333
TATCAAC115500.030.1882842
TCAACGC115400.030.1709694
CAACGCA118000.029.465935
AACGCAG124150.028.0828026
ACGCAGA143800.024.1132137
GTACATG111750.023.980731
CGCAGAG146000.023.6847938
TACATGG113800.023.5012232
ACATGGG115750.022.15973
GCAGAGT162250.021.078469
TGGTATC25400.020.9463652
AGAGTAC145700.020.30951910-11
GAGTACT89100.019.56408312-13
GGACCGA12400.019.5361776
GTACTTT89750.019.39593114-15
GTGGTAT29100.019.2672541
TAAGGTG19250.018.999895
Produced by FastQC (version 0.11.5)