##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938428_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3264256 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.816961966218336 31.0 31.0 34.0 30.0 34.0 2 32.07661990971297 33.0 31.0 34.0 30.0 34.0 3 32.137717446180694 33.0 31.0 34.0 30.0 34.0 4 35.78275049505921 37.0 35.0 37.0 35.0 37.0 5 35.67428718825975 37.0 35.0 37.0 33.0 37.0 6 35.705248301603795 37.0 35.0 37.0 33.0 37.0 7 35.6311003181123 37.0 35.0 37.0 33.0 37.0 8 35.59876737608815 37.0 35.0 37.0 33.0 37.0 9 37.14954004832954 39.0 37.0 39.0 33.0 39.0 10-11 37.07423253568348 39.0 37.0 39.0 33.0 39.0 12-13 36.954977949033406 39.0 37.0 39.0 33.0 39.0 14-15 37.976346830640736 40.0 37.0 41.0 33.0 41.0 16-17 38.068530470649364 40.0 37.0 41.0 33.0 41.0 18-19 38.19783543325033 40.0 37.5 41.0 33.0 41.0 20-21 38.143763234256134 40.0 37.0 41.0 33.0 41.0 22-23 38.017879571945336 40.0 37.0 41.0 33.0 41.0 24-25 37.88939133450317 40.0 37.0 41.0 32.5 41.0 26-27 37.66907237054937 40.0 37.0 41.0 32.0 41.0 28-29 37.52905960806996 39.5 36.5 41.0 32.0 41.0 30-31 37.27625789766489 39.0 36.0 41.0 31.0 41.0 32-33 37.17890462635283 39.0 36.0 41.0 31.0 41.0 34-35 37.04543868495608 39.0 36.0 41.0 31.0 41.0 36-37 36.914342808897345 39.0 36.0 41.0 30.5 41.0 38-39 36.697113369784724 39.0 35.0 40.5 30.0 41.0 40-41 36.473049295153324 39.0 35.0 40.0 30.0 41.0 42-43 36.20887623397185 38.5 35.0 40.0 30.0 41.0 44-45 35.918977096159125 38.0 35.0 40.0 29.0 41.0 46-47 35.508560603089954 38.0 34.5 40.0 27.5 41.0 48-49 35.09833435245275 38.0 34.0 40.0 26.5 41.0 50-51 34.69399121882597 38.0 33.0 40.0 25.5 41.0 52-53 34.07081169491609 37.5 33.0 40.0 24.0 41.0 54-55 33.6176373728041 37.0 32.0 40.0 23.0 41.0 56-57 33.19899128622265 36.5 32.0 39.0 22.0 40.5 58-59 32.640389417986825 36.0 31.0 39.0 20.0 40.0 60-61 32.11397191274214 35.5 31.0 39.0 18.0 40.0 62-63 31.56486547011019 35.0 30.0 38.5 14.5 40.0 64-65 30.87388596360089 34.5 29.0 38.0 9.5 40.0 66-67 30.18802554088895 34.0 28.0 37.5 7.5 39.5 68-69 29.423495736853972 34.0 27.5 37.0 4.0 39.0 70-71 28.608264946131676 33.0 26.0 36.0 2.0 39.0 72-73 27.997974883097402 32.5 26.0 35.5 2.0 38.5 74-75 27.25976041707513 32.0 24.5 35.0 2.0 37.5 76-77 26.111640140969335 30.5 23.0 34.0 2.0 36.0 78-79 26.580925178662458 32.0 24.0 35.0 2.0 36.0 80-81 26.1856994365638 32.0 23.5 35.0 2.0 36.0 82-83 25.469541604580034 31.0 20.5 34.0 2.0 36.0 84-85 24.666043962238255 31.0 18.5 34.0 2.0 35.0 86-87 23.72122698097208 30.0 10.0 34.0 2.0 35.0 88-89 22.713083930917183 29.0 4.5 34.0 2.0 35.0 90-91 21.816524960052156 29.0 2.0 34.0 2.0 35.0 92-93 20.70272061995138 28.0 2.0 33.0 2.0 35.0 94-95 19.496772771498314 25.5 2.0 33.0 2.0 34.0 96-97 18.100656474247117 24.5 2.0 32.0 2.0 34.0 98-99 16.316120273655006 10.5 2.0 32.0 2.0 34.0 100-101 12.162480209885498 2.0 2.0 27.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27234416413996954 1101 2 0.2333233205990055 1101 3 0.2900679879633401 1101 4 0.12118495559020204 1101 5 0.1372620245467644 1101 6 0.1836877078429069 1101 7 0.18592400117403685 1101 8 0.22186053082694457 1101 9 0.18029276437881236 1101 10-11 0.2839562570988434 1101 12-13 0.22632797707623098 1101 14-15 0.4185536931268956 1101 16-17 0.2822506013923558 1101 18-19 0.33780315040829123 1101 20-21 0.3726973258247952 1101 22-23 0.40550200409840187 1101 24-25 0.27541295569424307 1101 26-27 0.40493201454997774 1101 28-29 0.33573597756707585 1101 30-31 0.48216041560031186 1101 32-33 0.3944514924051461 1101 34-35 0.17685791468311862 1101 36-37 0.3623151319196367 1101 38-39 0.49461950045684233 1101 40-41 0.6013233267141871 1101 42-43 0.5636209891496193 1101 44-45 0.6392184295958785 1101 46-47 0.7215955685795734 1101 48-49 0.6618805632552736 1101 50-51 0.9157498530518211 1101 52-53 1.0808405436526982 1101 54-55 1.1522463065094541 1101 56-57 1.0726429182858368 1101 58-59 1.1363330870581407 1101 60-61 1.0603416711064781 1101 62-63 1.3540305075579262 1101 64-65 1.2058281642462134 1101 66-67 1.2742435508859522 1101 68-69 1.5584383562921467 1101 70-71 1.6806604381802543 1101 72-73 1.88129809456807 1101 74-75 1.8960726041784177 1101 76-77 1.7654437826154066 1101 78-79 1.813068616399466 1101 80-81 1.993866821905332 1101 82-83 2.2266132909605254 1101 84-85 2.497611649970885 1101 86-87 2.6458008538142472 1101 88-89 2.4758058737893727 1101 90-91 2.6788841937814887 1101 92-93 2.9215001672021934 1101 94-95 2.8198303870463555 1101 96-97 2.7089403605570475 1101 98-99 2.607773987563924 1101 100-101 2.100692371338587 1102 1 0.20456089115647913 1102 2 0.14026369595676869 1102 3 0.15835451920888488 1102 4 0.1801876517710781 1102 5 0.14338288988831493 1102 6 0.16529876733543603 1102 7 0.2036364389548595 1102 8 0.27906226704632076 1102 9 0.2420237856364551 1102 10-11 0.27311102975578905 1102 12-13 0.37751454044630606 1102 14-15 0.44810134066288043 1102 16-17 0.3647987773907957 1102 18-19 0.3581335711689988 1102 20-21 0.3721973213029841 1102 22-23 0.5127343215439168 1102 24-25 0.3770933699867953 1102 26-27 0.4587448476159963 1102 28-29 0.3303640131297598 1102 30-31 0.25470308481544635 1102 32-33 0.3483089341513974 1102 34-35 0.45194081133372066 1102 36-37 0.4469698835403122 1102 38-39 0.46842055124782433 1102 40-41 0.499863940666458 1102 42-43 0.4756151308444103 1102 44-45 0.42011750978022633 1102 46-47 0.38998213021956474 1102 48-49 0.46467928118331514 1102 50-51 0.6395623558288008 1102 52-53 0.5308613243380194 1102 54-55 0.5429219618874512 1102 56-57 0.6815937217335843 1102 58-59 0.8435056575758324 1102 60-61 0.9307416098457253 1102 62-63 1.1185863258155955 1102 64-65 1.2093453493028363 1102 66-67 1.1232302909606666 1102 68-69 1.420728765288274 1102 70-71 1.7310040567404528 1102 72-73 1.8281404822118006 1102 74-75 1.7417522761070323 1102 76-77 1.6898872160957836 1102 78-79 1.7664869998154984 1102 80-81 2.0719056678282435 1102 82-83 2.256546619758936 1102 84-85 2.2514866481523264 1102 86-87 2.2493737456559906 1102 88-89 2.07312696427795 1102 90-91 2.3042739053565846 1102 92-93 2.7924489165666806 1102 94-95 2.3823992709879 1102 96-97 2.4405790915865637 1102 98-99 2.4499279714591857 1102 100-101 2.2949227231711227 1103 1 0.2597534989329411 1103 2 0.22461599447699143 1103 3 0.2479151897118541 1103 4 0.13084150005452955 1103 5 0.09621347347599851 1103 6 0.18743334932329248 1103 7 0.24857945853196384 1103 8 0.2539652130025871 1103 9 0.309949819257497 1103 10-11 0.3449403931158983 1103 12-13 0.40440430916247294 1103 14-15 0.5243899194430952 1103 16-17 0.31868961563748144 1103 18-19 0.31232080884053204 1103 20-21 0.32188529086369755 1103 22-23 0.43701409139602276 1103 24-25 0.41202431096625247 1103 26-27 0.5385057378094018 1103 28-29 0.4619204055106607 1103 30-31 0.38794544811827336 1103 32-33 0.4859779284407111 1103 34-35 0.5881804668463246 1103 36-37 0.5262122293379221 1103 38-39 0.45796803575809264 1103 40-41 0.5477176301852325 1103 42-43 0.5506550360726976 1103 44-45 0.7778533492513233 1103 46-47 0.8755199693569224 1103 48-49 0.8846548138412942 1103 50-51 0.9961671586886993 1103 52-53 1.0677590807594441 1103 54-55 1.0940574509117695 1103 56-57 1.2614380471045123 1103 58-59 1.2698377710621358 1103 60-61 1.2612926606627894 1103 62-63 1.3651059277546373 1103 64-65 1.6905654417874487 1103 66-67 1.6056409282703292 1103 68-69 1.6545000524371467 1103 70-71 1.698523988424455 1103 72-73 1.8988198766912276 1103 74-75 1.73990647497045 1103 76-77 1.8650484781227696 1103 78-79 1.7326653090667925 1103 80-81 1.9702205436258993 1103 82-83 2.2481503009532204 1103 84-85 2.2462154381595916 1103 86-87 2.2562153826446583 1103 88-89 2.190486057294759 1103 90-91 2.3809757118375856 1103 92-93 2.65641523272949 1103 94-95 2.314130601344207 1103 96-97 2.3603645880220654 1103 98-99 2.1703338374563934 1103 100-101 1.6763996761298543 1104 1 0.16156008612285788 1104 2 0.18622792544201872 1104 3 0.21858623378036413 1104 4 0.14934820249663971 1104 5 0.08604601240542564 1104 6 0.09873515372905217 1104 7 0.14232111910880718 1104 8 0.15655838730970828 1104 9 0.12655231553998192 1104 10-11 0.18003586701326668 1104 12-13 0.23693887573060834 1104 14-15 0.36691243361330095 1104 16-17 0.3822013899031589 1104 18-19 0.32556808478044985 1104 20-21 0.30582924266865774 1104 22-23 0.3671891692912297 1104 24-25 0.29674576059885993 1104 26-27 0.4703956919630272 1104 28-29 0.3979041756752437 1104 30-31 0.38280072845014956 1104 32-33 0.49528808772141275 1104 34-35 0.3865892049257198 1104 36-37 0.4232695718478041 1104 38-39 0.4037683295239347 1104 40-41 0.5974262275624227 1104 42-43 0.6185952939903814 1104 44-45 0.6773464395734123 1104 46-47 0.666991852548648 1104 48-49 0.8153304389117366 1104 50-51 0.8758023327849571 1104 52-53 0.9565869805154819 1104 54-55 0.9222257954612871 1104 56-57 0.9916674789512072 1104 58-59 1.1391101552554943 1104 60-61 1.0369707216628257 1104 62-63 1.0432558210463831 1104 64-65 1.3869606295539967 1104 66-67 1.3090974941913096 1104 68-69 1.3903187611036003 1104 70-71 1.5010961664727276 1104 72-73 1.673649088940678 1104 74-75 1.5825698731286018 1104 76-77 1.772544324317451 1104 78-79 1.8132607666034524 1104 80-81 2.0467947176821575 1104 82-83 2.083062625666269 1104 84-85 2.1992581687275994 1104 86-87 2.3694203024953033 1104 88-89 2.1928061893439583 1104 90-91 2.5868205794793546 1104 92-93 2.5748448579962613 1104 94-95 2.5446218956226794 1104 96-97 2.6369041318851565 1104 98-99 2.73692878962855 1104 100-101 2.0610153264321642 1105 1 0.13831453144413075 1105 2 0.11104913714247999 1105 3 0.09995042058140768 1105 4 0.09631411803861312 1105 5 0.045043866418566836 1105 6 0.03646875608700384 1105 7 0.07908173348322833 1105 8 0.04597090363309775 1105 9 0.17289895305449932 1105 10-11 0.13505112267746 1105 12-13 0.19120958292203483 1105 14-15 0.25988555692933346 1105 16-17 0.08310759041672355 1105 18-19 0.11673746307697996 1105 20-21 0.14141685816264982 1105 22-23 0.13356708267490802 1105 24-25 0.11170316601111097 1105 26-27 0.1567144544966581 1105 28-29 0.19665129546560678 1105 30-31 0.3437415705698328 1105 32-33 0.32295576381624613 1105 34-35 0.36415911646747645 1105 36-37 0.36238317030396416 1105 38-39 0.36830255722350813 1105 40-41 0.38874517464039116 1105 42-43 0.4047279303905995 1105 44-45 0.5259147710642296 1105 46-47 0.514513334386848 1105 48-49 0.6222098119531481 1105 50-51 0.5641372420682345 1105 52-53 0.5345214110380567 1105 54-55 0.7023370581135637 1105 56-57 0.7966895891188912 1105 58-59 0.9348068452333322 1105 60-61 1.0654995606681155 1105 62-63 0.9209298243752109 1105 64-65 1.2047432328241143 1105 66-67 1.2026316322550734 1105 68-69 1.4247473503589028 1105 70-71 1.4834762697824004 1105 72-73 1.5306395400900392 1105 74-75 1.5299616027307401 1105 76-77 1.6182840306986392 1105 78-79 1.6805879847822958 1105 80-81 1.7934273989002882 1105 82-83 2.0143325191051034 1105 84-85 2.1612170803730457 1105 86-87 2.172827605880901 1105 88-89 2.1544870542065446 1105 90-91 2.157503384693694 1105 92-93 2.2261419409656362 1105 94-95 2.0961683770654282 1105 96-97 2.2640096068758844 1105 98-99 2.189921175856977 1105 100-101 1.9318050355375824 1106 1 0.1040916895826065 1106 2 0.0668694792139064 1106 3 0.12080626180941323 1106 4 0.14191094934336945 1106 5 0.10260969355376659 1106 6 0.019374725649043967 1106 7 0.11618367767960791 1106 8 0.07489688761406654 1106 9 -9.717144142982193E-4 1106 10-11 0.10921279684155394 1106 12-13 0.13669238290066943 1106 14-15 0.15975370143227252 1106 16-17 0.0805269019995336 1106 18-19 0.20505857189133536 1106 20-21 0.13902703148817608 1106 22-23 0.28128526787420327 1106 24-25 0.1734223719152368 1106 26-27 0.007471717217477192 1106 28-29 0.300095714583243 1106 30-31 0.3000285069357318 1106 32-33 0.2122000085605933 1106 34-35 0.14467179860361767 1106 36-37 0.18290508804944494 1106 38-39 0.2947804542107306 1106 40-41 0.37680081172743485 1106 42-43 0.38705164370229994 1106 44-45 0.2471180665322592 1106 46-47 -0.20846574289875974 1106 48-49 0.1903201757501165 1106 50-51 0.530987883668999 1106 52-53 0.36967003426613587 1106 54-55 0.17343614072819946 1106 56-57 0.40605059215741335 1106 58-59 0.6525548969777262 1106 60-61 0.843828283135629 1106 62-63 0.7597370429996424 1106 64-65 0.9265505985521614 1106 66-67 0.9755212070492796 1106 68-69 0.826460838650636 1106 70-71 0.7605177488399093 1106 72-73 1.066859122773721 1106 74-75 1.2041410923921987 1106 76-77 1.1930642773192446 1106 78-79 1.3653420649995667 1106 80-81 1.3713100487222043 1106 82-83 1.7604373089238692 1106 84-85 1.8269126116148762 1106 86-87 1.7676207101905526 1106 88-89 1.9436559083456721 1106 90-91 2.016279746252188 1106 92-93 2.0554345651068644 1106 94-95 1.9318115234592064 1106 96-97 2.0951895114805765 1106 98-99 2.371063888225681 1106 100-101 2.137202845012027 1107 1 0.03379438366836851 1107 2 0.027190476666682173 1107 3 0.04574368358663605 1107 4 0.09750712138769302 1107 5 0.006885108961242281 1107 6 0.020063086099604277 1107 7 -0.011057775898876798 1107 8 0.10695186148045366 1107 9 0.10522395361247305 1107 10-11 0.08493045373652564 1107 12-13 0.15651335661102905 1107 14-15 0.13343684533504785 1107 16-17 0.1566298643745867 1107 18-19 0.099083750722599 1107 20-21 0.14301126313274892 1107 22-23 0.11616317250431507 1107 24-25 0.0803650356785468 1107 26-27 0.16704342948654727 1107 28-29 0.2185044385641035 1107 30-31 0.15509645264021543 1107 32-33 0.2683827878477061 1107 34-35 0.204594729028166 1107 36-37 0.28690564260443097 1107 38-39 0.4386700468926321 1107 40-41 0.35844436345706754 1107 42-43 0.3692615368275156 1107 44-45 0.34052331394518376 1107 46-47 0.29902176689498106 1107 48-49 0.32854919508661595 1107 50-51 0.3469934512653552 1107 52-53 0.31114608325691506 1107 54-55 0.44376872108779963 1107 56-57 0.42043039787662195 1107 58-59 0.47842191910226717 1107 60-61 0.6348536502103244 1107 62-63 0.8632310785657715 1107 64-65 0.8431625728696517 1107 66-67 0.827343140631573 1107 68-69 1.1524988485613967 1107 70-71 0.9719092748327682 1107 72-73 1.258638650706672 1107 74-75 1.2875559554832599 1107 76-77 1.2460752395314962 1107 78-79 1.3744597459761252 1107 80-81 1.4867430849361725 1107 82-83 1.8425983605093528 1107 84-85 1.9774959542755859 1107 86-87 2.0654877085726753 1107 88-89 2.217300386029496 1107 90-91 2.358579572254204 1107 92-93 2.538730716122192 1107 94-95 2.2616018524951613 1107 96-97 2.1064324353929216 1107 98-99 2.354122021529168 1107 100-101 2.262418689004008 1108 1 0.014815037823794341 1108 2 0.052309180003973665 1108 3 0.03135937433910385 1108 4 0.021027481677357684 1108 5 -0.00866245817838518 1108 6 0.03629791003203309 1108 7 0.07020628511707372 1108 8 0.03537115856993012 1108 9 0.029932309762209286 1108 10-11 0.06849874998270167 1108 12-13 0.031870080976410975 1108 14-15 0.14438499348926825 1108 16-17 0.02523635907414956 1108 18-19 0.010457768729715156 1108 20-21 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62-63 -0.2022145628457963 2316 64-65 -0.18044943500051858 2316 66-67 -0.2192378811844904 2316 68-69 0.226812774850206 2316 70-71 -0.06850983146634348 2316 72-73 0.13194538812232537 2316 74-75 0.4758462712489724 2316 76-77 0.35364654919943916 2316 78-79 0.08322078742520134 2316 80-81 -0.20820692893589765 2316 82-83 0.31525383166350096 2316 84-85 0.36591769414748754 2316 86-87 0.5243946825347514 2316 88-89 0.43884651555138987 2316 90-91 0.5732270391797023 2316 92-93 0.7758572625189721 2316 94-95 1.1048705880662943 2316 96-97 0.9279313913815415 2316 98-99 0.6739372387268041 2316 100-101 0.27116114951102155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 11.0 4 51.0 5 91.0 6 237.0 7 608.0 8 1513.0 9 2731.0 10 4359.0 11 6964.0 12 10002.0 13 13935.0 14 19424.0 15 25563.0 16 32084.0 17 39877.0 18 41129.0 19 40346.0 20 42774.0 21 46793.0 22 51278.0 23 57493.0 24 64845.0 25 72638.0 26 82728.0 27 96842.0 28 112729.0 29 131559.0 30 152758.0 31 176561.0 32 206412.0 33 244739.0 34 293672.0 35 353056.0 36 397803.0 37 337259.0 38 101693.0 39 1689.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.56752615922409 18.94247115652145 15.057932094490658 23.432070589763796 2 16.344591857806666 20.19257696000843 38.90174696170582 24.561084220479078 3 19.75799941609095 23.377714709882063 29.203325646933543 27.66096022709344 4 13.71081189710611 16.741946710061956 35.50052446866913 34.04671692416281 5 14.297141117078457 36.942915165427124 33.79738114180697 14.962562575687441 6 33.62784659046349 35.50594683750294 16.676388126421458 14.18981844561211 7 30.06041805544663 31.594397008077795 19.67998220727786 18.66520272919771 8 26.997004825915656 35.128548631830085 18.927894944353238 18.946551597901017 9 26.353178687841584 16.765158199152022 19.260746758816754 37.620916354189646 10-11 25.54033752254725 26.274900007842522 26.813368804407496 21.371393665202728 12-13 26.3866099962748 23.219533026821427 27.211009185554076 23.182847791349698 14-15 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26.185103482084042 27.812843727499732 23.231827443050385 40-41 23.233285256127928 25.97610642041333 27.883824228920005 22.906784094538732 42-43 23.02758770808502 26.003157703984325 27.616092801632803 23.353161786297857 44-45 22.933604024711464 26.23836097534674 27.834386260298743 22.993648739643053 46-47 23.28216492956737 26.58321013009404 27.138513118158087 22.996111822180502 48-49 23.146652713512665 26.431551324405927 27.208803476197946 23.21299248588346 50-51 23.34285362422555 26.64411124617677 27.10063181319112 22.912403316406557 52-53 23.625046564975296 26.39359474256921 27.012265582111205 22.96909311034429 54-55 23.058516243824016 26.524589370441532 27.261005264292997 23.155889121441454 56-57 23.10036345188613 26.711247524703946 27.155177167477063 23.033211855932866 58-59 23.123906335777587 26.248155781899456 27.13086841228139 23.497069470041566 60-61 22.80418570112148 26.489420560152144 27.449639366520273 23.2567543722061 62-63 22.880297991334015 26.600364677280215 27.396656389694922 23.12268094169085 64-65 22.608231866795887 26.51964683502675 27.254301787529677 23.617819510647685 66-67 22.62037039589956 26.564615253120405 27.2242487294887 23.590765621491332 68-69 23.021718388384592 26.282134252471995 27.30185859358857 23.394288765554847 70-71 22.854280685792013 26.405412567339255 26.99487325591904 23.745433490949697 72-73 22.889406687237 26.466448014979253 26.986583127262385 23.657562170521356 74-75 22.79259040957572 26.812848011920632 26.75474901478315 23.63981256372049 76-77 22.841713395027842 26.515720580738765 27.07848281507333 23.564083209160067 78-79 22.957099565720334 26.577097507058273 27.061036266763388 23.404766660458005 80-81 22.959064728784643 26.807236943482593 26.850983579387737 23.382714748345027 82-83 22.96196334805865 26.83648773673343 26.758153555826507 23.443395359381412 84-85 22.861336597991205 26.7092119968824 26.81688504580815 23.612566359318247 86-87 23.009364408019387 26.72348044163279 26.63871013103247 23.628445019315343 88-89 22.82461914751784 26.843375642106505 26.41217478040938 23.919830429966275 90-91 22.69910495899771 26.82511347316102 26.67043583006965 23.805345737771614 92-93 22.733193637722717 26.476699017229127 26.824145896135086 23.96596144891307 94-95 22.550884880181712 26.426087051090207 26.785158208357156 24.237869860370918 96-97 22.686861167402668 26.225708469467 26.769048373978137 24.318381989152197 98-99 22.439110167829973 26.433956160301154 26.450529615324285 24.676404056544584 100-101 22.60996766490363 26.07472455430106 26.822153906480956 24.493153874314352 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 389.0 1 291.5 2 269.5 3 348.5 4 311.0 5 300.0 6 553.0 7 1277.5 8 1971.0 9 2202.5 10 2018.0 11 1651.5 12 1610.0 13 1824.0 14 2068.5 15 2323.5 16 2492.0 17 2699.0 18 2975.5 19 3307.5 20 3770.5 21 4338.5 22 5178.5 23 6225.0 24 7551.5 25 9586.0 26 12282.0 27 15629.0 28 19095.5 29 23072.5 30 28442.0 31 35459.0 32 44916.5 33 50124.0 34 52064.0 35 57214.5 36 64464.5 37 71408.0 38 77063.5 39 82325.0 40 88414.5 41 97352.0 42 105046.5 43 110885.0 44 116927.0 45 123324.0 46 126821.0 47 129816.0 48 131906.5 49 130177.5 50 128325.0 51 124930.5 52 121777.0 53 121350.0 54 122531.5 55 116060.0 56 104384.5 57 95040.5 58 87491.5 59 77743.5 60 63994.0 61 53303.0 62 45213.5 63 34971.0 64 25627.5 65 19441.0 66 14508.0 67 11240.0 68 8571.5 69 6328.5 70 4891.5 71 3673.0 72 2820.0 73 2387.5 74 1901.0 75 1460.5 76 1053.5 77 636.0 78 398.0 79 271.5 80 156.0 81 86.0 82 52.0 83 30.5 84 17.0 85 10.0 86 6.5 87 3.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023895184691396752 2 0.012805368206415185 3 0.0015011077562544115 4 0.0 5 2.757136695161164E-4 6 0.0 7 0.0 8 1.5317426084228688E-4 9 4.90157634695318E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001822773704023214 26-27 0.009236407928789898 28-29 2.1444396517920162E-4 30-31 0.0 32-33 3.523007999372598E-4 34-35 0.010155453493843619 36-37 0.001179441808485609 38-39 0.012437749980393696 40-41 0.012514337110814839 42-43 0.00537641655556427 44-45 6.739667477060622E-4 46-47 6.126970433691475E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.658713042114344E-5 66-67 5.820621912006901E-4 68-69 0.0012407115128225238 70-71 3.063485216845737E-5 72-73 2.45078817347659E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5317426084228688E-4 82-83 0.0011028546780644656 84-85 0.006372049251039133 86-87 0.0043348315818367185 88-89 0.0 90-91 0.0014704729040859541 92-93 4.90157634695318E-4 94-95 1.5317426084228686E-5 96-97 3.063485216845737E-5 98-99 0.0 100-101 3.063485216845737E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3264256.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.418294516291816 #Duplication Level Percentage of deduplicated Percentage of total 1 74.49717935040016 37.56020729121488 2 13.761543733415236 13.876671299003185 3 4.850519402936086 7.336647474426584 4 2.1818531720419596 4.40021263277268 5 1.2017155377002426 3.0294223952287407 6 0.7656453127526817 2.3161518524029834 7 0.484630449063602 1.710396849871601 8 0.3596389923546174 1.4505907708862014 9 0.2492064667347421 1.1308108531677035 >10 1.4767125669812764 13.542422422823538 >50 0.10733319594057897 3.7103071016312197 >100 0.05738627677780115 5.433723509335609 >500 0.003591715883128359 1.269796521174404 >1k 0.00292207393717977 2.8058847580222483 >5k 1.2175308071582374E-4 0.4267542680384754 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7613 0.23322312955846602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6291 0.19272385499176534 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4518 0.13840826209709042 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3569 0.10933578738922438 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3554 0.10887626460669751 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3378 0.10348453062504902 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.063485216845737E-5 0.0 0.0 0.0 0.0 3 3.063485216845737E-5 0.0 0.0 0.0 0.0 4 3.063485216845737E-5 0.0 0.0 0.0 0.0 5 6.126970433691475E-5 0.0 0.0 0.0 0.0 6 6.126970433691475E-5 0.0 0.0 0.0 0.0 7 6.126970433691475E-5 0.0 0.0 3.063485216845737E-5 0.0 8 6.126970433691475E-5 0.0 0.0 3.063485216845737E-5 0.0 9 6.126970433691475E-5 0.0 0.0 9.190455650537213E-5 0.0 10-11 6.126970433691475E-5 0.0 0.0 1.5317426084228688E-4 0.0 12-13 9.190455650537213E-5 0.0 0.0 1.5317426084228688E-4 3.063485216845737E-5 14-15 9.190455650537213E-5 0.0 0.0 1.5317426084228688E-4 3.063485216845737E-5 16-17 9.190455650537213E-5 0.0 0.0 1.5317426084228688E-4 3.063485216845737E-5 18-19 9.190455650537213E-5 0.0 0.0 1.5317426084228688E-4 3.063485216845737E-5 20-21 9.190455650537213E-5 0.0 0.0 2.1444396517920162E-4 3.063485216845737E-5 22-23 1.225394086738295E-4 0.0 0.0 4.748402086110893E-4 3.063485216845737E-5 24-25 1.9912653909497294E-4 0.0 0.0 7.352364520429771E-4 3.063485216845737E-5 26-27 2.1444396517920162E-4 0.0 0.0 0.001087537251980237 3.063485216845737E-5 28-29 2.1444396517920162E-4 0.0 0.0 0.0024814230256450477 3.063485216845737E-5 30-31 2.1444396517920162E-4 0.0 0.0 0.008884107128852639 3.063485216845737E-5 32-33 2.1444396517920162E-4 0.0 0.0 0.023405027056701437 3.063485216845737E-5 34-35 2.1444396517920162E-4 0.0 0.0 0.04524767665281154 3.063485216845737E-5 36-37 2.1444396517920162E-4 0.0 0.0 0.08191759469845503 3.063485216845737E-5 38-39 2.1444396517920162E-4 0.0 0.0 0.13897500686220687 3.063485216845737E-5 40-41 2.1444396517920162E-4 0.0 0.0 0.1995860618774998 3.063485216845737E-5 42-43 2.297613912634303E-4 0.0 0.0 0.264103060544271 3.063485216845737E-5 44-45 2.45078817347659E-4 0.0 0.0 0.3434473276605756 3.063485216845737E-5 46-47 2.757136695161164E-4 0.0 0.0 0.4396713983217002 3.063485216845737E-5 48-49 2.757136695161164E-4 0.0 0.0 0.5460662399027527 3.063485216845737E-5 50-51 3.0634852168457376E-4 0.0 0.0 0.6621416947690377 3.063485216845737E-5 52-53 3.216659477688024E-4 0.0 0.0 0.7713089904713356 3.063485216845737E-5 54-55 3.369833738530311E-4 0.0 0.0 0.8937564945886597 3.063485216845737E-5 56-57 3.369833738530311E-4 0.0 0.0 1.041125450945024 3.063485216845737E-5 58-59 3.369833738530311E-4 0.0 0.0 1.1995535889342013 3.063485216845737E-5 60-61 3.6761822602148853E-4 0.0 0.0 1.3710168565210572 3.063485216845737E-5 62-63 3.8293565210571724E-4 0.0 0.0 1.5377776743000549 3.063485216845737E-5 64-65 3.982530781899459E-4 0.0 0.0 1.717083464042036 3.063485216845737E-5 66-67 4.5952278252686066E-4 0.0 0.0 1.9297352903693827 3.063485216845737E-5 68-69 4.90157634695318E-4 0.0 0.0 2.165838096031684 6.126970433691475E-5 70-71 4.90157634695318E-4 0.0 0.0 2.4177484854129085 9.190455650537213E-5 72-73 4.90157634695318E-4 0.0 0.0 2.676995921888479 9.190455650537213E-5 74-75 4.90157634695318E-4 0.0 0.0 2.944070563093091 9.190455650537213E-5 76-77 4.90157634695318E-4 0.0 0.0 3.233677138067603 9.190455650537213E-5 78-79 4.90157634695318E-4 0.0 0.0 3.55514395929731 9.190455650537213E-5 80-81 5.207924868637754E-4 0.0 0.0 3.8980551770449376 9.190455650537213E-5 82-83 5.207924868637754E-4 0.0 0.0 4.250922721747314 9.190455650537213E-5 84-85 5.207924868637754E-4 3.063485216845737E-5 0.0 4.618449043212298 9.190455650537213E-5 86-87 5.207924868637754E-4 3.063485216845737E-5 0.0 4.995165820327818 9.190455650537213E-5 88-89 5.361099129480041E-4 3.063485216845737E-5 0.0 5.3991322984471815 9.190455650537213E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4910 0.0 50.418163 1 GTATCAA 8685 0.0 40.97725 1 ATCAACG 11230 0.0 30.919233 3 TATCAAC 11550 0.0 30.188284 2 TCAACGC 11540 0.0 30.170969 4 CAACGCA 11800 0.0 29.46593 5 AACGCAG 12415 0.0 28.082802 6 ACGCAGA 14380 0.0 24.113213 7 GTACATG 11175 0.0 23.98073 1 CGCAGAG 14600 0.0 23.684793 8 TACATGG 11380 0.0 23.501223 2 ACATGGG 11575 0.0 22.1597 3 GCAGAGT 16225 0.0 21.07846 9 TGGTATC 2540 0.0 20.946365 2 AGAGTAC 14570 0.0 20.309519 10-11 GAGTACT 8910 0.0 19.564083 12-13 GGACCGA 1240 0.0 19.536177 6 GTACTTT 8975 0.0 19.395931 14-15 GTGGTAT 2910 0.0 19.267254 1 TAAGGTG 1925 0.0 18.99989 5 >>END_MODULE