FastQCFastQC Report
Thu 26 May 2016
SRR938427_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938427_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120825
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72320.2317335960843687No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA56250.18024080171108603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54870.1758188940424407No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA51940.16643035094886768No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA50200.16085490214927142No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48710.15608052357950222No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC41910.1342914133282065No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA41650.13345830028918634No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG41530.13307378657886937No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA41200.13201637387549767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39450.12640888226670832No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36080.1156104555686397No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34040.10907372249325098No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32730.10487611448895724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39950.056.9029961
GTATCAA76800.045.9480131
ATCAACG102550.034.2267463
TATCAAC103850.033.889892
TCAACGC104400.033.620244
CAACGCA106250.033.034855
AACGCAG110800.031.6518576
ACGCAGA128900.026.8391047
CGCAGAG129650.026.6838468
TAAGGTG20750.025.900335
TGGTATC18550.024.8698252
TACATGG101450.023.909092
GTACATG105300.023.7564431
GTAAGGT21500.023.227144
AGGTAAG18650.022.951372
GCAGAGT147250.022.6546559
GTGGTAT21700.022.3544731
ACATGGG104250.022.0351493
AGAGTAC128000.020.8637110-11
GAGTACT84350.020.70137212-13
Produced by FastQC (version 0.11.5)