FastQCFastQC Report
Thu 26 May 2016
SRR938427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938427_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120825
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73350.23503400543125616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58820.18847580367370806No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA54600.1749537381942275No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA54590.1749216953850344No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC51320.16444369677889661No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA51070.16364262654906955No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48210.15447838311984813No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG45040.1443208126056411No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43570.1396105196542581No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39830.12762650901604544No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38850.1244863137151234No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36270.11621926894330825No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33150.1062219124750667No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA32440.10394687302235786No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31270.10019786434676728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.056.8991281
GTATCAA80550.045.9061931
ATCAACG107650.033.8907783
TATCAAC108400.033.717412
TCAACGC108650.033.5788544
CAACGCA110700.032.957025
AACGCAG117000.031.2090076
ACGCAGA134250.027.1635447
TGGTATC18650.027.013712
TAAGGTG24800.027.0085815
CGCAGAG134800.026.9822628
AGGTAAG21500.026.0855942
GTAAGGT24100.026.0190374
AAGGTAA21350.024.710541
GCAGAGT155200.023.0990839
GTACATG102450.022.8713671
GGTAAGG26600.022.859293
GTGGTAT24550.022.8448161
TACATGG100350.022.7816332
GTGTAAG15650.021.2589021
Produced by FastQC (version 0.11.5)