##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938427_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3120825 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.785652832183796 31.0 31.0 34.0 30.0 34.0 2 32.01546353928849 31.0 31.0 34.0 30.0 34.0 3 32.07832095679828 33.0 31.0 34.0 30.0 34.0 4 35.7573074427435 37.0 35.0 37.0 35.0 37.0 5 35.64441261525398 37.0 35.0 37.0 33.0 37.0 6 35.645698172758806 37.0 35.0 37.0 33.0 37.0 7 35.58112005639534 37.0 35.0 37.0 33.0 37.0 8 35.55223506604824 37.0 35.0 37.0 33.0 37.0 9 37.14366169202054 39.0 37.0 39.0 33.0 39.0 10-11 37.04092363397499 39.0 37.0 39.0 33.0 39.0 12-13 36.93640864194564 39.0 37.0 39.0 33.0 39.0 14-15 37.98717438497833 40.0 37.0 41.0 33.0 41.0 16-17 37.88857049017487 40.0 37.0 41.0 33.0 41.0 18-19 38.1230697011207 40.0 37.0 41.0 33.0 41.0 20-21 38.08030648946993 40.0 37.0 41.0 33.0 41.0 22-23 37.97950589988224 40.0 37.0 41.0 33.0 41.0 24-25 37.846829924779506 40.0 37.0 41.0 32.0 41.0 26-27 37.64664776140924 40.0 37.0 41.0 32.0 41.0 28-29 37.534926021164274 39.5 36.5 41.0 32.0 41.0 30-31 37.27651085850697 39.0 36.0 41.0 31.0 41.0 32-33 37.07984667515801 39.0 36.0 41.0 31.0 41.0 34-35 36.99521777094198 39.0 36.0 41.0 31.0 41.0 36-37 36.94659953698141 39.0 36.0 41.0 30.5 41.0 38-39 36.7701718295643 39.0 35.0 41.0 30.0 41.0 40-41 36.59776613555711 39.0 35.0 40.5 30.0 41.0 42-43 36.38330793940705 39.0 35.0 40.0 30.0 41.0 44-45 36.08893962333678 38.5 35.0 40.0 29.5 41.0 46-47 35.720808920718085 38.0 35.0 40.0 28.5 41.0 48-49 35.35205162096561 38.0 34.0 40.0 27.0 41.0 50-51 34.96512316454783 38.0 34.0 40.0 26.0 41.0 52-53 34.45990451242861 38.0 33.0 40.0 24.5 41.0 54-55 34.10435125327437 37.0 33.0 40.0 24.0 41.0 56-57 33.74816739163522 37.0 33.0 40.0 23.0 41.0 58-59 33.24754928584589 36.0 32.0 39.0 22.5 40.5 60-61 32.83429814231813 36.0 31.0 39.0 22.0 40.0 62-63 32.33727860421523 35.0 31.0 39.0 20.0 40.0 64-65 31.78828995538039 35.0 30.5 38.0 18.0 40.0 66-67 31.27504297741783 34.5 30.0 38.0 17.5 40.0 68-69 30.75993847780635 34.0 29.0 37.0 13.0 39.0 70-71 30.160382911569855 34.0 29.0 36.5 8.0 39.0 72-73 29.661948042584893 34.0 28.0 36.0 7.5 39.0 74-75 29.07918595243245 33.5 27.5 35.5 7.0 38.0 76-77 27.873418567205785 31.5 26.0 34.5 2.0 36.0 78-79 28.563542012128202 33.0 27.0 35.0 2.0 37.0 80-81 28.203470556663703 33.0 26.5 35.0 2.0 36.0 82-83 27.76240753646872 33.0 26.0 35.0 2.0 36.0 84-85 27.21095527624907 32.0 25.5 35.0 2.0 35.5 86-87 26.60163802840595 32.0 25.0 34.5 2.0 35.0 88-89 25.826391098507607 31.5 23.5 34.0 2.0 35.0 90-91 25.20448807606963 31.0 20.5 34.0 2.0 35.0 92-93 24.370831911433676 31.0 18.0 34.0 2.0 35.0 94-95 23.53464116059055 30.5 6.0 34.0 2.0 35.0 96-97 22.44588770597518 30.0 2.0 34.0 2.0 35.0 98-99 21.040214206179456 29.0 2.0 34.0 2.0 35.0 100-101 17.072176107279326 22.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2585027132152611 1101 2 0.2708423005997034 1101 3 0.2799264715852132 1101 4 0.24003127098313115 1101 5 0.05447509558175767 1101 6 0.2543655617193963 1101 7 0.21117714349561112 1101 8 0.2752654913177963 1101 9 0.24863694696502847 1101 10-11 0.26853997981417876 1101 12-13 0.32434723973220514 1101 14-15 0.448227674938785 1101 16-17 0.41969370431858266 1101 18-19 0.4606589964293448 1101 20-21 0.3693232067540251 1101 22-23 0.3909719805227283 1101 24-25 0.4599143399600507 1101 26-27 0.42913241683880443 1101 28-29 0.3141073216464818 1101 30-31 0.4773469582305765 1101 32-33 0.4039758319316107 1101 34-35 0.45390363661077515 1101 36-37 0.5620503830642747 1101 38-39 0.6566492321091957 1101 40-41 0.7070225016653993 1101 42-43 0.7291496994834503 1101 44-45 0.6461924535994683 1101 46-47 0.6537203764873567 1101 48-49 0.6273694766145681 1101 50-51 0.5903905095118915 1101 52-53 0.6991648056215212 1101 54-55 0.6211194764310122 1101 56-57 0.5604352683813403 1101 58-59 0.7863247756662872 1101 60-61 0.7151820942340379 1101 62-63 0.7749663137257627 1101 64-65 0.7921770602755984 1101 66-67 0.7060243498755092 1101 68-69 0.7659766834835473 1101 70-71 0.6858589260603729 1101 72-73 0.7379134883234002 1101 74-75 0.7277816700442052 1101 76-77 0.6628407479475449 1101 78-79 0.8759216836996551 1101 80-81 1.009068363482001 1101 82-83 0.8062290023207694 1101 84-85 0.8805319825071258 1101 86-87 0.9862421005486546 1101 88-89 1.0518330159784348 1101 90-91 0.9732308729521577 1101 92-93 1.446429571306595 1101 94-95 1.371936066483702 1101 96-97 1.4686932295453374 1101 98-99 1.6473827836476467 1101 100-101 1.8551603033179092 1102 1 0.29412485473874384 1102 2 0.24695082950002245 1102 3 0.2602973935469777 1102 4 0.25108899911933946 1102 5 0.2017587741373177 1102 6 0.21505021086397846 1102 7 0.23575909803256678 1102 8 0.30131250393775133 1102 9 0.2968856332658092 1102 10-11 0.32295485862131557 1102 12-13 0.39101041234360423 1102 14-15 0.4896286684907736 1102 16-17 0.5405136350618704 1102 18-19 0.3687351330985038 1102 20-21 0.44989778677904013 1102 22-23 0.4274240310459092 1102 24-25 0.45720550805497595 1102 26-27 0.3899704181555492 1102 28-29 0.3652929811081336 1102 30-31 0.4250792756088728 1102 32-33 0.4099174977221125 1102 34-35 0.4434073315742211 1102 36-37 0.6015589523411293 1102 38-39 0.5999822823035714 1102 40-41 0.46469279460153246 1102 42-43 0.4476168517671013 1102 44-45 0.51863140588042 1102 46-47 0.5905201989071358 1102 48-49 0.6337061372027861 1102 50-51 0.761838982172101 1102 52-53 0.7487588981025084 1102 54-55 0.6515811711846098 1102 56-57 0.710459618686393 1102 58-59 0.8014521568374917 1102 60-61 0.7266763312222011 1102 62-63 0.8788057205670086 1102 64-65 0.8174000109457467 1102 66-67 0.7561695002313336 1102 68-69 0.8994006744395442 1102 70-71 0.8903092645673532 1102 72-73 1.0274298979626124 1102 74-75 1.2068751179145103 1102 76-77 1.156430786615683 1102 78-79 1.253404832767643 1102 80-81 1.1977295410799762 1102 82-83 1.3365156576625843 1102 84-85 1.326882695150882 1102 86-87 1.260265210003407 1102 88-89 1.122518108435706 1102 90-91 1.2833725916212515 1102 92-93 1.6148098842683467 1102 94-95 1.5283022865668698 1102 96-97 1.3593830570499215 1102 98-99 1.416373549880145 1102 100-101 1.6377868682941035 1103 1 0.20738275968255238 1103 2 0.22999483660461806 1103 3 0.2187346846228806 1103 4 0.1960276362767459 1103 5 0.12692423139191078 1103 6 0.1357594847326027 1103 7 0.16992586949064048 1103 8 0.24031042714999273 1103 9 0.3420563112577568 1103 10-11 0.2645291472984397 1103 12-13 0.2547593268065498 1103 14-15 0.5067540036116185 1103 16-17 0.39940625819937026 1103 18-19 0.403671673045487 1103 20-21 0.3834796096560851 1103 22-23 0.33629261867992 1103 24-25 0.40405019122225383 1103 26-27 0.36303132008873007 1103 28-29 0.44779892881968664 1103 30-31 0.4033517920861769 1103 32-33 0.3732914749173588 1103 34-35 0.4388802390470232 1103 36-37 0.3632335359857777 1103 38-39 0.2503434057656122 1103 40-41 0.3661767757185572 1103 42-43 0.4820239056923654 1103 44-45 0.40233512716933717 1103 46-47 0.3980204889193004 1103 48-49 0.4713712691979737 1103 50-51 0.6033237739754966 1103 52-53 0.5635697754403637 1103 54-55 0.5467987124474547 1103 56-57 0.4864185067273823 1103 58-59 0.6059048569673919 1103 60-61 0.6663103633388587 1103 62-63 0.6678908858666901 1103 64-65 0.6408759542420626 1103 66-67 0.7288419050785713 1103 68-69 0.8218312573448223 1103 70-71 0.4655796289936909 1103 72-73 0.4544459905019451 1103 74-75 0.505307020310358 1103 76-77 0.6051676760700921 1103 78-79 0.6638436846740028 1103 80-81 0.6956445635236541 1103 82-83 0.753333429566748 1103 84-85 0.81351455966632 1103 86-87 0.7472247701262233 1103 88-89 0.6793893649073333 1103 90-91 0.8120890314598697 1103 92-93 1.0859843853411597 1103 94-95 0.9680134997590386 1103 96-97 1.1409085718487368 1103 98-99 1.2182512322335235 1103 100-101 1.5028424702873124 1104 1 0.1714011742539192 1104 2 0.2033459367249364 1104 3 0.24520715782543334 1104 4 0.21396986486385572 1104 5 0.15309586312491064 1104 6 0.11183874611655398 1104 7 0.1946722967765524 1104 8 0.1535727287980393 1104 9 0.2581556247993859 1104 10-11 0.2699192728815376 1104 12-13 0.2854396895421587 1104 14-15 0.3121595652976126 1104 16-17 0.4089840300535812 1104 18-19 0.3907277570273422 1104 20-21 0.32749635157252754 1104 22-23 0.39232394835390494 1104 24-25 0.28764074696727704 1104 26-27 0.25885427320927334 1104 28-29 0.3816264144607615 1104 30-31 0.38830563055341116 1104 32-33 0.3742077903560457 1104 34-35 0.4442370691433055 1104 36-37 0.4844993929613608 1104 38-39 0.40662877606363423 1104 40-41 0.4647399868062294 1104 42-43 0.5540433735043919 1104 44-45 0.5335828824329383 1104 46-47 0.4457852289199664 1104 48-49 0.5252063280846855 1104 50-51 0.6411269574574661 1104 52-53 0.6426599043530032 1104 54-55 0.5439142692612791 1104 56-57 0.5348296027537387 1104 58-59 0.6226253973078357 1104 60-61 0.7474092050302446 1104 62-63 0.4953729314231836 1104 64-65 0.5600278137753065 1104 66-67 0.5883325373244048 1104 68-69 0.6960829215773714 1104 70-71 0.797980885711155 1104 72-73 0.6626408471175935 1104 74-75 0.6773789334394813 1104 76-77 0.693589012921322 1104 78-79 0.8795675571243144 1104 80-81 0.9383935847052953 1104 82-83 1.041728494505751 1104 84-85 1.0601336886524457 1104 86-87 1.1610805786027036 1104 88-89 1.11198156828641 1104 90-91 1.015099347347352 1104 92-93 1.4021695578955118 1104 94-95 1.509261288515134 1104 96-97 1.5404965254233964 1104 98-99 1.7658194993098242 1104 100-101 1.6296134601857766 1105 1 0.2220458027668606 1105 2 0.2371180304917715 1105 3 0.18026298997681067 1105 4 0.1344419760969089 1105 5 0.07932812737794137 1105 6 0.11598227858181076 1105 7 0.259425872372546 1105 8 0.22008032205027916 1105 9 0.2281330780203632 1105 10-11 0.15935528480407157 1105 12-13 0.278532711646875 1105 14-15 0.4419681082682061 1105 16-17 0.30898966248134485 1105 18-19 0.2537079997224012 1105 20-21 0.29345529515664737 1105 22-23 0.3688271949649149 1105 24-25 0.27937544368948153 1105 26-27 0.15189219424036793 1105 28-29 0.23802397055604274 1105 30-31 0.28523518936363956 1105 32-33 0.36987963942518576 1105 34-35 0.38932658776416673 1105 36-37 0.43647455452386197 1105 38-39 0.26639298784519383 1105 40-41 0.2475561324164346 1105 42-43 0.42663287932428773 1105 44-45 0.42741958412875647 1105 46-47 0.34244497168301535 1105 48-49 0.6269225686496611 1105 50-51 0.7185779596029178 1105 52-53 0.6366208323722873 1105 54-55 0.5959165119916108 1105 56-57 0.4208299759731986 1105 58-59 0.5124642131553188 1105 60-61 0.6350522274478791 1105 62-63 0.5822774515318017 1105 64-65 0.6189179751756484 1105 66-67 0.49921686261929565 1105 68-69 0.7400231120217029 1105 70-71 0.6208782975821485 1105 72-73 0.6292756820334091 1105 74-75 0.5818354638981127 1105 76-77 0.6402617273361848 1105 78-79 1.0099077671399108 1105 80-81 0.923132550125036 1105 82-83 0.8428787614202413 1105 84-85 1.0544854730226305 1105 86-87 1.0355266520893842 1105 88-89 1.1199884341589623 1105 90-91 1.1685467344982499 1105 92-93 1.3733574141536806 1105 94-95 1.2380476596329544 1105 96-97 1.3710740939100212 1105 98-99 1.5755164064242635 1105 100-101 1.4865179347798296 1106 1 -0.026547366684653184 1106 2 0.021316913699237716 1106 3 0.03309169853389449 1106 4 0.03126603491750046 1106 5 -0.0871566860977353 1106 6 -0.10774906363736392 1106 7 0.07341530349297898 1106 8 0.036479058348888316 1106 9 -0.1971515043947889 1106 10-11 0.048787403643352434 1106 12-13 -0.052232284109877014 1106 14-15 0.05362668068499232 1106 16-17 -0.00801011644222882 1106 18-19 -0.03544057076889118 1106 20-21 0.05933179187972115 1106 22-23 0.07600590316543077 1106 24-25 0.11006484557076845 1106 26-27 0.01981585868913527 1106 28-29 0.07814192552776689 1106 30-31 0.0985498381730352 1106 32-33 0.008948265827406487 1106 34-35 -7.693308797058762E-4 1106 36-37 0.17174580103833392 1106 38-39 0.005110316671419923 1106 40-41 0.0768574595881546 1106 42-43 0.07874472973399094 1106 44-45 0.0693139951740136 1106 46-47 -0.49385378101803923 1106 48-49 -0.1600336368230515 1106 50-51 0.09037060417620069 1106 52-53 0.21539307373069505 1106 54-55 0.1961541334944954 1106 56-57 0.01661283613165665 1106 58-59 -0.025308905377301016 1106 60-61 0.11184291085930198 1106 62-63 -0.16058527426923774 1106 64-65 0.010307832759636426 1106 66-67 0.09077245620950691 1106 68-69 -0.1855490123801431 1106 70-71 -0.20273936856391828 1106 72-73 0.21765932808291666 1106 74-75 0.27268304239807506 1106 76-77 0.11864330525136069 1106 78-79 0.36581671217745537 1106 80-81 -0.11325848415607354 1106 82-83 0.40849324691834354 1106 84-85 0.5414172887732569 1106 86-87 0.6563189614822811 1106 88-89 0.7504714504972227 1106 90-91 0.8136118094146312 1106 92-93 0.9829919248972807 1106 94-95 0.9766956703016767 1106 96-97 1.1643194245619135 1106 98-99 1.0739929758886184 1106 100-101 0.9475084998083894 1107 1 0.13810471268162416 1107 2 0.18785988849111135 1107 3 0.1741256416191348 1107 4 0.1683416546944514 1107 5 0.013290831930156344 1107 6 0.06216003497929279 1107 7 0.1157488132671105 1107 8 0.16820260998760972 1107 9 0.2131744527965509 1107 10-11 0.20173165064214515 1107 12-13 0.23304783284515906 1107 14-15 0.2630254758463977 1107 16-17 0.23839619693038117 1107 18-19 0.20782092595320734 1107 20-21 0.2597318771212471 1107 22-23 0.2843241015444633 1107 24-25 0.260352673217902 1107 26-27 0.3169038064076588 1107 28-29 0.3117799285845493 1107 30-31 0.205640741847823 1107 32-33 0.33863229930992134 1107 34-35 0.33418354567818653 1107 36-37 0.3677200635959963 1107 38-39 0.2722693219119421 1107 40-41 0.38682595230253014 1107 42-43 0.3246900972464246 1107 44-45 0.2711204739885176 1107 46-47 0.3145255071235269 1107 48-49 0.3339209514397439 1107 50-51 0.29802900562255985 1107 52-53 0.3765875092003341 1107 54-55 0.3773769828071849 1107 56-57 0.3190320457227003 1107 58-59 0.35334207868115897 1107 60-61 0.2984813469980381 1107 62-63 0.5334330618252849 1107 64-65 0.3035392887170403 1107 66-67 0.361087093692408 1107 68-69 0.5449903694354532 1107 70-71 0.5460874376055536 1107 72-73 0.501959809055954 1107 74-75 0.5187916520216582 1107 76-77 0.5698095331074668 1107 78-79 0.8308640647634569 1107 80-81 0.7078162007137827 1107 82-83 0.840686268570149 1107 84-85 1.0547537842923482 1107 86-87 1.132771584836167 1107 88-89 1.156949852539352 1107 90-91 1.407216769921682 1107 92-93 1.4459450397648226 1107 94-95 1.5205298434473846 1107 96-97 1.5819784905255645 1107 98-99 1.6627039269045234 1107 100-101 1.6742972085137957 1108 1 0.17155950295241595 1108 2 0.10663078358589928 1108 3 0.12716567029347914 1108 4 0.12242407606892414 1108 5 0.13838617976877998 1108 6 0.16359193197415323 1108 7 0.15747723176028927 1108 8 0.14600272247842128 1108 9 0.2500700352820999 1108 10-11 0.16428696624890904 1108 12-13 0.2455236037523605 1108 14-15 0.3342359311532874 1108 16-17 0.3262944990989638 1108 18-19 0.2944216196927627 1108 20-21 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-1.079631355289031 2316 74-75 -1.0528521559771669 2316 76-77 -1.0295352753716536 2316 78-79 -1.072863885093632 2316 80-81 -1.30079588511148 2316 82-83 -1.2098498120936405 2316 84-85 -1.1661206842808944 2316 86-87 -1.2602870627174951 2316 88-89 -1.4543652153112951 2316 90-91 -1.206888776986581 2316 92-93 -1.5232007579597777 2316 94-95 -1.628331504388676 2316 96-97 -1.777202644147149 2316 98-99 -1.6429194071887032 2316 100-101 -1.8037884480448092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 46.0 5 102.0 6 218.0 7 615.0 8 1438.0 9 2528.0 10 4256.0 11 6447.0 12 9120.0 13 12445.0 14 16124.0 15 21241.0 16 26271.0 17 32311.0 18 31446.0 19 28990.0 20 29007.0 21 30716.0 22 33846.0 23 38482.0 24 44201.0 25 51380.0 26 60696.0 27 72830.0 28 86481.0 29 102731.0 30 121495.0 31 145155.0 32 174215.0 33 214564.0 34 270745.0 35 351288.0 36 448810.0 37 457203.0 38 187325.0 39 6040.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.35319827429307 18.81006457302083 15.018290135118853 23.818447017567244 2 16.309073142206422 20.17925200949992 38.32338842466491 25.188286423628742 3 19.474209543950717 23.16563729142134 29.637035078865363 27.72311808576258 4 13.843092520555494 16.526553918521415 35.303830403547785 34.326523157375306 5 14.347185784151556 36.844082671036674 33.78642631645086 15.022305228360915 6 33.96653128579782 35.3733067378017 16.302452075973502 14.35770990042697 7 29.913596565010852 32.013522065479485 19.624009676928374 18.44887169258129 8 27.3645270080828 34.38709315645703 19.07165573205803 19.176724103402147 9 26.529843871412208 16.505443272211675 19.259138208646753 37.705574647729364 10-11 25.60422324225165 25.975487250967294 26.73982040005447 21.680469106726584 12-13 26.52282649619892 23.110443552586254 26.96929497809073 23.397434973124092 14-15 22.927495133498354 24.72403931652688 25.018224347728562 27.330241202246203 16-17 22.912258777727043 27.840234553363292 26.095343378754016 23.15216329015565 18-19 23.105893473680837 26.22470019946649 28.127562423397663 22.541843903455018 20-21 23.254091466198844 25.52482116107119 28.476204208822985 22.74488316390698 22-23 23.225541323207803 25.35174192721476 28.45752325106342 22.965193498514015 24-25 23.171130315296722 25.647708657521463 28.307039379404582 22.874121647777233 26-27 22.455320600436785 26.06506456837538 28.15239165184523 23.327223179342607 28-29 22.83102747642367 26.43985348727146 27.94674862800526 22.78237040829961 30-31 22.82946247512438 25.882452652408784 27.768569777425583 23.51951509504125 32-33 22.481616963790195 25.875941902299786 27.84546137351433 23.796979760395693 34-35 23.470915365346738 25.9990094388524 27.591502324902457 22.938572870898412 36-37 23.63693267826403 25.44150352699629 27.59553690501869 23.326026889720993 38-39 22.923518788164664 25.83268497203181 27.621489235706996 23.622307004096534 40-41 23.450078457773632 25.615141308907013 27.792204338200587 23.142575895118764 42-43 23.131468136641526 25.693600860554366 27.534343188030935 23.64058781477317 44-45 23.037502113003846 25.94725461716376 27.8761407216499 23.139102548182496 46-47 23.65464260251696 26.334286606906826 26.915046502126845 23.096024288449367 48-49 23.221984571387374 26.361314716461195 26.846298655003086 23.57040205714835 50-51 23.46681581602719 26.4934028324066 26.95602326191702 23.08375808964919 52-53 23.879711294289173 26.022654266099508 26.995153525109544 23.102480914501776 54-55 23.12462439296055 26.40188042642524 27.162603341502763 23.310891839111452 56-57 23.17762130205955 26.533977393798114 26.952953145402258 23.33544815874008 58-59 23.114922467793804 26.037834479480644 26.921767173099624 23.925475879625928 60-61 22.62398564482148 26.38698100662485 27.3242331755225 23.66480017303117 62-63 22.89975268445918 26.3999045027131 27.44646503813883 23.253877774688892 64-65 22.550456104727186 26.444732233964547 27.08825267535291 23.91655898595536 66-67 22.450039038823 26.47686198682534 27.141058731597955 23.932040242753704 68-69 23.249533977685733 25.940357155848147 27.25379255553289 23.55631631093323 70-71 22.873183510554355 26.19801427220822 26.80322370351002 24.1255785137274 72-73 22.84983068574197 26.286022537637493 26.834066900280185 24.030079876340352 74-75 22.852426004837316 26.682792117417296 26.65532522071671 23.80945665702868 76-77 22.996847160288294 26.155040720506186 27.02138638067498 23.82672573853054 78-79 23.042244438001834 26.444133853037055 26.93247393428123 23.581147774679877 80-81 23.2730661427342 26.378167272703962 26.663023498435095 23.685743086126738 82-83 23.132328407539717 26.47323039177425 26.71958685327422 23.674854347411813 84-85 22.96646316890229 26.584283695507438 26.686403797271808 23.762849338318464 86-87 23.264068034945527 26.609686737505424 26.27794651778521 23.848298709763842 88-89 22.981762951562697 26.718920346464316 26.082540631402463 24.21677607057053 90-91 22.815495550703773 26.6288231832628 26.391839345855377 24.163841920178047 92-93 22.977740741334273 26.194516113523818 26.581288761398053 24.246454383743853 94-95 22.688529539782856 25.922190718989523 26.856112350086313 24.5331673911413 96-97 23.020749638615122 25.48207756996778 26.848392091733658 24.648780699683435 98-99 22.606147003514923 25.942879255253747 26.358972912153856 25.09200082907747 100-101 22.74925289428354 25.5031526659456 26.85641950086127 24.891174938909586 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 279.0 1 203.5 2 187.5 3 266.5 4 248.5 5 242.0 6 438.0 7 1007.0 8 1598.5 9 1731.5 10 1529.5 11 1338.5 12 1385.0 13 1533.0 14 1703.5 15 1959.5 16 2199.5 17 2484.5 18 2847.0 19 3207.0 20 3753.0 21 4487.0 22 5444.0 23 6736.5 24 8417.5 25 10662.5 26 13812.0 27 17942.0 28 21630.5 29 25154.5 30 29418.5 31 36520.5 32 48605.5 33 52584.0 34 49935.5 35 52665.5 36 58633.5 37 64653.0 38 69277.0 39 73769.5 40 79376.5 41 86331.0 42 90719.0 43 94325.5 44 98554.5 45 105451.5 46 109940.0 47 112829.0 48 114735.5 49 113504.5 50 113801.0 51 113657.0 52 115105.5 53 120768.5 54 126928.0 55 122047.5 56 111845.5 57 102994.0 58 96435.5 59 86615.0 60 69756.5 61 57721.5 62 49776.5 63 38045.5 64 26663.5 65 19966.5 66 14968.0 67 11661.5 68 9214.5 69 7146.5 70 5476.0 71 3821.5 72 2746.0 73 2204.5 74 1689.5 75 1199.0 76 860.5 77 607.0 78 405.0 79 238.0 80 133.0 81 87.0 82 53.5 83 30.5 84 18.0 85 7.0 86 5.0 87 2.5 88 1.5 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.447277562823933E-4 2 0.05258224988584749 3 0.0 4 1.6021404596540978E-4 5 0.044122948258873855 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006696947121354129 26-27 0.0020347183837607042 28-29 1.2817123677232782E-4 30-31 0.001554076245864475 32-33 4.1655651951006545E-4 34-35 0.011903903615229947 36-37 0.023968021276425302 38-39 0.004389864859452228 40-41 0.0012176267493371144 42-43 0.00147396922288177 44-45 0.008571451459149424 46-47 0.0 48-49 0.0 50-51 5.126849470893113E-4 52-53 0.0 54-55 0.001393862199899065 56-57 0.0 58-59 0.023535443352318698 60-61 0.0 62-63 0.010173591918803522 64-65 0.015668933695417078 66-67 0.006841139762722998 68-69 0.017126881513702306 70-71 1.441926413688688E-4 72-73 3.2042809193081955E-5 74-75 0.022606201885719324 76-77 0.05041936026531446 78-79 0.031097546321886042 80-81 0.02659553163025802 82-83 0.037121594450185445 84-85 0.015364527008082798 86-87 0.009965313659048488 88-89 0.08457699486513982 90-91 0.025025433979797007 92-93 0.02483317712463852 94-95 0.027652944333629727 96-97 0.03936459109370118 98-99 0.05432858298687046 100-101 0.014419264136886881 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3120825.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.26489855996965 #Duplication Level Percentage of deduplicated Percentage of total 1 70.67379221646108 31.28368243310023 2 14.885540104047706 13.178138454320637 3 5.631908398998569 7.478875619421382 4 2.7872161676236 4.935033636982641 5 1.5477862716622435 3.4256301153821416 6 0.9630091656438942 2.557650181772879 7 0.655386841016014 2.030744242457897 8 0.4592681775631603 1.6263567433324335 9 0.34254864555792935 1.3646592942729068 >10 1.8255329675955274 14.842211916211182 >50 0.1411079167550842 4.278656279364658 >100 0.07610598174232604 6.081589735205922 >500 0.005869589073769432 1.6765558851351499 >1k 0.0044927718832080505 4.137598019342343 >5k 4.347843757943275E-4 1.1026174436976663 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7335 0.23503400543125616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5882 0.18847580367370806 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5460 0.1749537381942275 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5459 0.1749216953850344 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5132 0.16444369677889661 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5107 0.16364262654906955 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4821 0.15447838311984813 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4504 0.1443208126056411 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4357 0.1396105196542581 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3983 0.12762650901604544 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3885 0.1244863137151234 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3627 0.11621926894330825 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3315 0.1062219124750667 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3244 0.10394687302235786 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3127 0.10019786434676728 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.408561838616391E-5 2 0.0 0.0 0.0 0.0 6.408561838616391E-5 3 0.0 0.0 0.0 0.0 6.408561838616391E-5 4 0.0 0.0 0.0 0.0 6.408561838616391E-5 5 0.0 0.0 0.0 0.0 6.408561838616391E-5 6 0.0 0.0 0.0 0.0 9.612842757924587E-5 7 0.0 0.0 0.0 0.0 9.612842757924587E-5 8 0.0 0.0 0.0 0.0 9.612842757924587E-5 9 0.0 0.0 0.0 0.0 9.612842757924587E-5 10-11 0.0 0.0 0.0 0.0 1.1214983217578684E-4 12-13 0.0 0.0 0.0 0.0 3.044066873342786E-4 14-15 0.0 0.0 0.0 0.0 3.5247090112390154E-4 16-17 0.0 0.0 0.0 3.2042809193081955E-5 4.005351149135245E-4 18-19 0.0 0.0 0.0 3.2042809193081955E-5 4.4859932870314743E-4 20-21 0.0 0.0 0.0 1.2817123677232782E-4 4.4859932870314743E-4 22-23 0.0 0.0 0.0 2.883852827377376E-4 4.4859932870314743E-4 24-25 0.0 0.0 0.0 6.408561838616392E-4 4.4859932870314743E-4 26-27 0.0 0.0 0.0 0.0012656909631267373 4.4859932870314743E-4 28-29 0.0 0.0 0.0 0.0035247090112390154 4.4859932870314743E-4 30-31 0.0 0.0 0.0 0.011759710973861078 4.646207332996884E-4 32-33 0.0 0.0 0.0 0.0320588305976785 5.287063516858523E-4 34-35 0.0 0.0 0.0 0.06290003444601988 5.767705654754753E-4 36-37 0.0 0.0 0.0 0.11514583483534001 6.088133746685572E-4 38-39 0.0 0.0 0.0 0.19300986117452917 6.728989930547211E-4 40-41 0.0 0.0 0.0 0.2768338500236316 6.728989930547211E-4 42-43 0.0 0.0 0.0 0.36874864819398717 6.889203976512622E-4 44-45 0.0 0.0 0.0 0.48189180745475957 7.690274206339669E-4 46-47 0.0 0.0 0.0 0.6176571900058478 8.491344436166719E-4 48-49 0.0 0.0 0.0 0.7535026795799188 8.651558482132128E-4 50-51 0.0 0.0 0.0 0.9059143015068132 8.971986574062949E-4 52-53 0.0 0.0 0.0 1.0524300465421803 9.292414665993768E-4 54-55 0.0 0.0 0.0 1.2114424871628495 9.292414665993768E-4 56-57 0.0 0.0 0.0 1.3987807711102032 9.292414665993768E-4 58-59 0.0 0.0 0.0 1.5987279004750348 9.292414665993768E-4 60-61 0.0 0.0 0.0 1.821265210320989 9.612842757924587E-4 62-63 0.0 0.0 0.0 2.0334526927975776 0.0011375197263544094 64-65 0.0 0.0 0.0 2.263183613307379 0.0011535411309509505 66-67 0.0 0.0 0.0 2.5333846018280424 0.0011855839401440325 68-69 0.0 0.0 0.0 2.8263199634711977 0.0012176267493371144 70-71 0.0 0.0 0.0 3.1302299872629833 0.0012817123677232784 72-73 0.0 0.0 0.0 3.4536060176395664 0.0013938621998990652 74-75 0.0 0.0 0.0 3.7864667195373016 0.0014419264136886882 76-77 0.0 0.0 0.0 4.1464516594169805 0.001457947818285229 78-79 0.0 0.0 0.0 4.5424366954250885 0.001506012032074852 80-81 0.0 0.0 0.0 4.960867719272949 0.0015380548412679339 82-83 0.0 0.0 0.0 5.395848854069037 0.0016181618642506388 84-85 0.0 0.0 0.0 5.843935497825094 0.0016982688872333438 86-87 0.0 0.0 0.0 6.304983457899754 0.0017463331010229668 88-89 0.0 0.0 0.0 6.789294497448591 0.0017943973148125897 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4210 0.0 56.899128 1 GTATCAA 8055 0.0 45.906193 1 ATCAACG 10765 0.0 33.890778 3 TATCAAC 10840 0.0 33.71741 2 TCAACGC 10865 0.0 33.578854 4 CAACGCA 11070 0.0 32.95702 5 AACGCAG 11700 0.0 31.209007 6 ACGCAGA 13425 0.0 27.163544 7 TGGTATC 1865 0.0 27.01371 2 TAAGGTG 2480 0.0 27.008581 5 CGCAGAG 13480 0.0 26.982262 8 AGGTAAG 2150 0.0 26.085594 2 GTAAGGT 2410 0.0 26.019037 4 AAGGTAA 2135 0.0 24.71054 1 GCAGAGT 15520 0.0 23.099083 9 GTACATG 10245 0.0 22.871367 1 GGTAAGG 2660 0.0 22.85929 3 GTGGTAT 2455 0.0 22.844816 1 TACATGG 10035 0.0 22.781633 2 GTGTAAG 1565 0.0 21.258902 1 >>END_MODULE