FastQCFastQC Report
Thu 26 May 2016
SRR938426_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938426_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3133554
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75580.2411957796163717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57530.1835934533121178No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA56270.1795724598969732No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA53320.17015822928215057No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA50850.1622758056826211No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA47040.15011708749873148No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC43090.13751159226871468No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG42410.13534153233038268No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA41820.13345868620741816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39440.12586347642325615No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39150.12493800968484986No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA35690.1138962341162782No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33230.10604572316290065No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32710.10438626556299971No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41850.056.7511831
GTATCAA77750.044.2596241
ATCAACG104900.032.7098853
TCAACGC105400.032.5998084
TATCAAC105200.032.5262572
CAACGCA107750.032.0185785
AACGCAG112450.030.7166866
TGGTATC19500.028.270972
TAAGGTG20000.026.611585
ACGCAGA128800.026.5919087
CGCAGAG130550.026.1630928
GTAAGGT20150.025.7080294
AGGTAAG19000.024.5126252
AAGGTAA19200.023.550071
GTACATG103850.023.4198571
TACATGG101300.023.3634642
GTGGTAT25100.023.3239021
ACATGGG101950.022.3754273
GCAGAGT146900.022.3442389
GGTAAGG23200.021.5089233
Produced by FastQC (version 0.11.5)