##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938426_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3133554 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.111930415113317 31.0 31.0 34.0 28.0 34.0 2 31.20753814997284 31.0 31.0 34.0 28.0 34.0 3 31.275784939401078 31.0 31.0 34.0 27.0 34.0 4 34.91558307276658 37.0 35.0 37.0 32.0 37.0 5 34.930188214404474 37.0 35.0 37.0 32.0 37.0 6 34.963155573511735 37.0 35.0 37.0 32.0 37.0 7 34.88934768636506 37.0 35.0 37.0 32.0 37.0 8 34.83883603090931 37.0 35.0 37.0 32.0 37.0 9 36.360272074456034 39.0 35.0 39.0 32.0 39.0 10-11 36.21670601495937 39.0 35.0 39.0 32.0 39.0 12-13 36.075284963973814 39.0 35.0 39.0 30.0 39.0 14-15 37.08312127379965 39.0 36.0 41.0 31.0 41.0 16-17 36.93977078422775 39.0 36.0 41.0 31.0 41.0 18-19 36.92377600641316 39.0 36.0 41.0 31.0 41.0 20-21 36.9942072164705 39.0 36.0 41.0 31.0 41.0 22-23 36.923805844737316 39.0 36.0 41.0 30.0 41.0 24-25 36.80158551599877 39.0 36.0 41.0 30.0 41.0 26-27 36.61678991330611 39.0 36.0 41.0 30.0 41.0 28-29 36.44439524578163 39.0 36.0 41.0 30.0 41.0 30-31 36.22207084990397 39.0 35.0 40.0 29.0 41.0 32-33 35.94772501128112 38.5 35.0 40.0 28.5 41.0 34-35 35.706660711766894 38.0 35.0 40.0 27.0 41.0 36-37 35.416577630383905 38.0 34.0 40.0 26.5 41.0 38-39 35.1536510939336 38.0 34.0 40.0 25.5 41.0 40-41 35.099450974835605 38.0 34.0 40.0 25.0 41.0 42-43 34.915887519410866 38.0 34.0 40.0 25.0 41.0 44-45 34.640364423271464 38.0 33.0 40.0 24.0 41.0 46-47 34.29812139825897 38.0 33.0 40.0 23.5 41.0 48-49 33.92908068601977 38.0 33.0 40.0 22.5 41.0 50-51 33.164634948049404 37.0 32.0 39.5 21.0 40.5 52-53 33.21252641569285 37.0 32.0 39.5 21.0 40.0 54-55 33.338881506430084 37.0 32.0 40.0 20.5 41.0 56-57 33.03053242420587 37.0 32.0 40.0 18.5 41.0 58-59 32.6923258702419 36.5 31.5 39.5 17.5 41.0 60-61 32.23854766823868 36.0 31.0 39.0 13.5 41.0 62-63 31.716307585572167 35.0 30.5 39.0 9.5 40.0 64-65 31.215569286503438 35.0 30.0 38.5 7.5 40.0 66-67 30.681724329627 35.0 29.0 38.0 6.0 40.0 68-69 30.12445963911903 34.0 29.0 37.5 2.0 39.5 70-71 29.50068085630565 34.0 28.0 36.5 2.0 39.0 72-73 28.96745755777625 34.0 27.5 36.0 2.0 39.0 74-75 28.380607131710512 34.0 26.0 35.5 2.0 38.5 76-77 27.670455336017824 33.0 25.5 35.0 2.0 37.5 78-79 26.91412993042405 32.5 24.5 35.0 2.0 37.0 80-81 26.289438924620416 32.0 23.5 35.0 2.0 36.0 82-83 25.645471404035163 31.5 20.5 35.0 2.0 36.0 84-85 25.01804628227246 31.0 19.0 34.0 2.0 35.0 86-87 24.365485324331416 31.0 16.0 34.0 2.0 35.0 88-89 23.577020852361247 30.5 7.5 34.0 2.0 35.0 90-91 22.753462043417795 30.0 2.0 34.0 2.0 35.0 92-93 21.923864723569466 29.0 2.0 34.0 2.0 35.0 94-95 20.969207966417684 29.0 2.0 34.0 2.0 35.0 96-97 19.74183020302187 27.0 2.0 33.5 2.0 35.0 98-99 18.26100746947396 24.5 2.0 33.0 2.0 34.5 100-101 14.802123244086427 8.5 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6218174757639012 1101 2 0.5828375340922349 1101 3 0.6742370076686548 1101 4 0.5862976585520911 1101 5 0.5648414928766599 1101 6 0.6573234391941654 1101 7 0.5557337374497209 1101 8 0.6295599277842143 1101 9 0.6244682811037165 1101 10-11 0.6936550926965737 1101 12-13 0.7048322340396638 1101 14-15 0.7380261855729486 1101 16-17 0.8099843062421499 1101 18-19 0.7193328864055459 1101 20-21 0.8701305805757258 1101 22-23 0.9617589137301934 1101 24-25 0.9507901688388145 1101 26-27 0.8411895344553884 1101 28-29 0.8620048082705054 1101 30-31 0.9638839589913317 1101 32-33 0.9486997839792721 1101 34-35 0.870019995530285 1101 36-37 0.8748697026642489 1101 38-39 0.781021137588894 1101 40-41 0.8151484787311603 1101 42-43 0.8753878314588945 1101 44-45 0.9832499734370188 1101 46-47 1.0615632798280004 1101 48-49 1.0994026207200491 1101 50-51 0.9345203829461681 1101 52-53 0.9944275305798556 1101 54-55 1.0725285660782475 1101 56-57 0.9513875017736737 1101 58-59 0.948366962031443 1101 60-61 1.0745770869770297 1101 62-63 0.9717752481006592 1101 64-65 0.9009007252004864 1101 66-67 0.7501064137552156 1101 68-69 0.7398905708012293 1101 70-71 0.959882958995582 1101 72-73 1.0438013558376333 1101 74-75 1.0441814444048738 1101 76-77 1.108462783518224 1101 78-79 0.9878703763069581 1101 80-81 1.0594043136788294 1101 82-83 0.9947591604635271 1101 84-85 1.0211889029122396 1101 86-87 1.1618337535081906 1101 88-89 1.289539229815965 1101 90-91 1.3733506892391105 1101 92-93 1.3380773710435712 1101 94-95 1.1488708621188408 1101 96-97 1.3060680174492418 1101 98-99 1.6487533479153704 1101 100-101 1.4667998178490738 1102 1 0.6529738811960009 1102 2 0.685118648946279 1102 3 0.629285970708036 1102 4 0.5919352349554146 1102 5 0.5895832653531698 1102 6 0.6563655844797083 1102 7 0.5969397309356239 1102 8 0.5238673608419475 1102 9 0.6888489508294668 1102 10-11 0.772098372235746 1102 12-13 0.8610312772579363 1102 14-15 0.9368646244425207 1102 16-17 0.9429668267616691 1102 18-19 0.9750468862456714 1102 20-21 0.8911327818067605 1102 22-23 0.9491718744368995 1102 24-25 0.8850582876848989 1102 26-27 0.8275806904745551 1102 28-29 0.6531041737601626 1102 30-31 0.6801898608978689 1102 32-33 0.7060403771367305 1102 34-35 0.8268073033920018 1102 36-37 0.812158223805028 1102 38-39 0.9905792979971011 1102 40-41 0.9559107924540626 1102 42-43 0.9683647617207001 1102 44-45 1.0593798412397675 1102 46-47 1.1459627053512236 1102 48-49 0.9456069591248522 1102 50-51 0.9813779016550157 1102 52-53 0.9628706330763208 1102 54-55 0.9700787778052344 1102 56-57 0.9882621721657543 1102 58-59 1.0720623047888154 1102 60-61 0.9996640390901987 1102 62-63 0.8807637601746983 1102 64-65 0.916579520365822 1102 66-67 0.8774412718018354 1102 68-69 0.8143801110642244 1102 70-71 1.020754266649135 1102 72-73 1.0393182052070102 1102 74-75 0.9793199159468706 1102 76-77 0.9940412598457371 1102 78-79 0.9888289284624001 1102 80-81 0.9884346507520583 1102 82-83 1.182956742274694 1102 84-85 0.9560253825091429 1102 86-87 0.960874396613157 1102 88-89 1.4208125907357712 1102 90-91 1.50914534544631 1102 92-93 1.229475455870638 1102 94-95 1.1589965785382645 1102 96-97 1.3128800308165012 1102 98-99 1.451490642820776 1102 100-101 1.0938522122980867 1103 1 0.5774472408923721 1103 2 0.6137401584529876 1103 3 0.5682455724269033 1103 4 0.5080916914691471 1103 5 0.5369325967063858 1103 6 0.601308786987218 1103 7 0.577951508625226 1103 8 0.46947660918932854 1103 9 0.5283408792310667 1103 10-11 0.5529962183475376 1103 12-13 0.6467017549035816 1103 14-15 0.6965639042358305 1103 16-17 0.6407392961189728 1103 18-19 0.6930107953329667 1103 20-21 0.6412342186502116 1103 22-23 0.715948018776075 1103 24-25 0.776240983140795 1103 26-27 0.7704302344932472 1103 28-29 0.6441841573996996 1103 30-31 0.7831206155900219 1103 32-33 0.8383838895136577 1103 34-35 0.8289325812085906 1103 36-37 0.853135852620639 1103 38-39 0.7530035518574678 1103 40-41 0.7355731898314986 1103 42-43 0.8344614985040764 1103 44-45 0.8924652858432154 1103 46-47 0.7685229427205797 1103 48-49 0.7476697122707208 1103 50-51 0.9910477040750223 1103 52-53 0.9091295566116031 1103 54-55 0.8554352551265296 1103 56-57 1.025274686279701 1103 58-59 1.0180315544825689 1103 60-61 0.9275985422026594 1103 62-63 1.1029433416367986 1103 64-65 0.9588722582222147 1103 66-67 1.1275671881248535 1103 68-69 1.0334131104226607 1103 70-71 1.2412274243898018 1103 72-73 1.2494073207124146 1103 74-75 0.9874640858018182 1103 76-77 1.1458512113661676 1103 78-79 1.1632301246929693 1103 80-81 1.363252049134168 1103 82-83 1.3982733486436452 1103 84-85 1.3232871895231249 1103 86-87 1.2048459677775512 1103 88-89 1.0846447206646843 1103 90-91 1.2274376875511166 1103 92-93 1.2885262844500218 1103 94-95 1.1006430071351936 1103 96-97 1.160835484283563 1103 98-99 1.528349374804133 1103 100-101 1.1065702709121616 1104 1 0.5927614806363906 1104 2 0.5543192455759218 1104 3 0.6101099115658251 1104 4 0.5580631278997501 1104 5 0.5428307318893744 1104 6 0.61476516055329 1104 7 0.5691280018640654 1104 8 0.3920411971166473 1104 9 0.5393077891307314 1104 10-11 0.49244994230438977 1104 12-13 0.6034148157314476 1104 14-15 0.6443357681030193 1104 16-17 0.6387745016809063 1104 18-19 0.6851740794266377 1104 20-21 0.6824317124808559 1104 22-23 0.7460047332092898 1104 24-25 0.7241077791613648 1104 26-27 0.7743936395550648 1104 28-29 0.7967489645151389 1104 30-31 0.7057915887438213 1104 32-33 0.5807631035287386 1104 34-35 0.776614893892102 1104 36-37 0.7027339978546863 1104 38-39 0.7616398107466793 1104 40-41 0.6248185665911947 1104 42-43 0.7109327000479553 1104 44-45 0.6787260608647685 1104 46-47 0.8199900418921473 1104 48-49 0.75736222095383 1104 50-51 0.5075916681771346 1104 52-53 0.554909553345496 1104 54-55 0.6608288824391835 1104 56-57 0.8541364008876755 1104 58-59 0.7414993258310432 1104 60-61 0.8361538002196696 1104 62-63 0.8496438692482862 1104 64-65 0.8402797836416198 1104 66-67 0.930313378348945 1104 68-69 0.9076415830388562 1104 70-71 1.0088112485432639 1104 72-73 0.7681313180402931 1104 74-75 0.8079128270794662 1104 76-77 1.0296837275490631 1104 78-79 0.9886052177738698 1104 80-81 1.1033902620558784 1104 82-83 1.0271361196988842 1104 84-85 1.2443273832933208 1104 86-87 1.2057362467447952 1104 88-89 1.092142583580486 1104 90-91 1.0157642119008443 1104 92-93 1.1119775574492259 1104 94-95 1.146539519141271 1104 96-97 1.1587385352045203 1104 98-99 1.1039217865626476 1104 100-101 0.8151819254408732 1105 1 0.4654878907587445 1105 2 0.5689233725295324 1105 3 0.5339339651895152 1105 4 0.4702736034311954 1105 5 0.41496768085177393 1105 6 0.3871491486308045 1105 7 0.3282088777489349 1105 8 0.36428965208405373 1105 9 0.433885214209738 1105 10-11 0.5294777445382977 1105 12-13 0.6259585310779343 1105 14-15 0.6969639105156915 1105 16-17 0.6251547987292341 1105 18-19 0.6784638635922136 1105 20-21 0.6313234252678868 1105 22-23 0.618550582071066 1105 24-25 0.6906449043798162 1105 26-27 0.8625209467466206 1105 28-29 0.7788365046999317 1105 30-31 0.7214030976603425 1105 32-33 0.6772243891993739 1105 34-35 0.7758860621582997 1105 36-37 0.7586036038913022 1105 38-39 0.7665109554917038 1105 40-41 0.7816546120981656 1105 42-43 0.9465764092945861 1105 44-45 0.8094827218474734 1105 46-47 0.8303589739383952 1105 48-49 0.7675971281468676 1105 50-51 0.7283661600019542 1105 52-53 0.790513885632059 1105 54-55 0.9051108944042454 1105 56-57 0.988901397758049 1105 58-59 0.9993520701283387 1105 60-61 1.0325963854139886 1105 62-63 1.0270406063922302 1105 64-65 1.0399151004886846 1105 66-67 1.0790217329179939 1105 68-69 0.9277987204970941 1105 70-71 0.9493899361669413 1105 72-73 0.939360955020998 1105 74-75 0.8675622871759963 1105 76-77 0.8172804433292171 1105 78-79 0.9432989466421624 1105 80-81 0.9850088966270931 1105 82-83 1.0359955538791752 1105 84-85 0.8512493851244223 1105 86-87 1.093946727621958 1105 88-89 1.1237594187439512 1105 90-91 0.9503607517495141 1105 92-93 1.130884178114691 1105 94-95 1.0085180715373063 1105 96-97 0.8929301176227895 1105 98-99 1.129626159238036 1105 100-101 0.833749474682838 1106 1 0.3263748594263589 1106 2 0.3615669877444141 1106 3 -0.044325589779273145 1106 4 0.2213048791043022 1106 5 0.34496455434192086 1106 6 0.08193487696021151 1106 7 0.2533061799574199 1106 8 0.28842373471918137 1106 9 0.30216987390163297 1106 10-11 0.14221144553522436 1106 12-13 0.17596626547001648 1106 14-15 0.4848195486722844 1106 16-17 0.22573497639472606 1106 18-19 -0.07072753449109825 1106 20-21 0.2238972671888959 1106 22-23 0.30551788341955444 1106 24-25 0.34706219921043413 1106 26-27 0.1632867966121907 1106 28-29 0.2231463550214272 1106 30-31 0.33723471980426467 1106 32-33 0.4480173875766056 1106 34-35 0.20853331015117504 1106 36-37 0.1306182712558268 1106 38-39 0.16850764519060846 1106 40-41 0.31581606035069143 1106 42-43 -0.14423218513569935 1106 44-45 0.1831401404021733 1106 46-47 0.0021902618143414543 1106 48-49 0.3503892801860218 1106 50-51 -0.08733946594711739 1106 52-53 0.16880562278400646 1106 54-55 -0.07177918187005616 1106 56-57 -0.31566291359516896 1106 58-59 -0.5767597756382514 1106 60-61 0.19424433778397798 1106 62-63 0.20954727373766602 1106 64-65 0.35927357734966137 1106 66-67 -0.2561539266834103 1106 68-69 -0.48237251510689916 1106 70-71 -0.4291596608445616 1106 72-73 0.06444394883806837 1106 74-75 0.1501224168105857 1106 76-77 0.14658666789123487 1106 78-79 0.33001097094079057 1106 80-81 0.21896808093461573 1106 82-83 0.12711071659103723 1106 84-85 0.07935958163600176 1106 86-87 0.09491822903076041 1106 88-89 0.10386565457300279 1106 90-91 0.12474976986727171 1106 92-93 0.11349508429066546 1106 94-95 -0.16924422705851683 1106 96-97 -0.010457358950613838 1106 98-99 0.07018256008149137 1106 100-101 -0.22706348151411504 1107 1 0.4231064745331494 1107 2 0.45667143860044135 1107 3 0.4357021031378565 1107 4 0.41972116188239283 1107 5 0.44415622054460613 1107 6 0.4000891632012866 1107 7 0.4503834545143448 1107 8 0.3218657160497358 1107 9 0.5233852588961625 1107 10-11 0.4482481637511171 1107 12-13 0.4184475106837695 1107 14-15 0.4990002572787233 1107 16-17 0.6106942721877502 1107 18-19 0.5584965253051521 1107 20-21 0.6204103236599892 1107 22-23 0.623278179954653 1107 24-25 0.6918685450323565 1107 26-27 0.5888681972677361 1107 28-29 0.6444649267094817 1107 30-31 0.7341941123490798 1107 32-33 0.7373060976470995 1107 34-35 0.7045869781848353 1107 36-37 0.6909038533227942 1107 38-39 0.7508789675958596 1107 40-41 0.7628828838891195 1107 42-43 0.735309968978946 1107 44-45 0.6605699109270162 1107 46-47 0.6426714376588833 1107 48-49 0.6963800595581731 1107 50-51 0.6270456536280449 1107 52-53 0.5635266262278265 1107 54-55 0.5702747581639116 1107 56-57 0.6126897411917156 1107 58-59 0.5374454403783702 1107 60-61 0.6701503589397575 1107 62-63 0.7949327794072296 1107 64-65 0.8237021439536818 1107 66-67 0.8070958654371623 1107 68-69 0.6930701927748117 1107 70-71 0.711359713700773 1107 72-73 0.7304257467235864 1107 74-75 0.7797775240727205 1107 76-77 0.7686554310291669 1107 78-79 0.8453376405708575 1107 80-81 0.7758149584532426 1107 82-83 0.7593397864869011 1107 84-85 0.642102841641826 1107 86-87 0.5767637146015083 1107 88-89 0.9471891423471348 1107 90-91 0.9187714678246195 1107 92-93 0.9435420362899158 1107 94-95 1.0301602930754363 1107 96-97 1.1401915169487893 1107 98-99 1.062607899191942 1107 100-101 0.8604379218431717 1108 1 0.3531212439904472 1108 2 0.29148246586333215 1108 3 0.29927765856372446 1108 4 0.31360148887548434 1108 5 0.30749115502477764 1108 6 0.35405559298800426 1108 7 0.29893636194261575 1108 8 0.25455290433113476 1108 9 0.34843991486656734 1108 10-11 0.266089353926958 1108 12-13 0.39538740482850443 1108 14-15 0.46612901193064715 1108 16-17 0.41267580363233236 1108 18-19 0.4038225981109491 1108 20-21 0.4212693314577223 1108 22-23 0.49626152054551653 1108 24-25 0.3625794542293761 1108 26-27 0.39038936158580384 1108 28-29 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-1.1590885067456895 2316 52-53 -1.0979477302071814 2316 54-55 -1.153000769553394 2316 56-57 -1.0899056155040014 2316 58-59 -1.0934677434108764 2316 60-61 -1.096882429410428 2316 62-63 -1.2428227418981486 2316 64-65 -1.1722820178479978 2316 66-67 -1.1869783065627963 2316 68-69 -1.0326759538501307 2316 70-71 -1.2297631290678908 2316 72-73 -1.161405217912236 2316 74-75 -1.1668145732264392 2316 76-77 -1.0716350127112335 2316 78-79 -1.073662209264743 2316 80-81 -0.9716239094361718 2316 82-83 -0.8460612952495126 2316 84-85 -1.003637878797207 2316 86-87 -0.9799308537771871 2316 88-89 -1.0605477788405082 2316 90-91 -1.1693315965424702 2316 92-93 -1.2480535940182342 2316 94-95 -1.312420434107171 2316 96-97 -1.4286076837712613 2316 98-99 -1.5252565143331722 2316 100-101 -1.1863391171912792 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9560.0 3 3278.0 4 3432.0 5 3926.0 6 4683.0 7 5763.0 8 7574.0 9 9683.0 10 12198.0 11 15107.0 12 18652.0 13 21557.0 14 24149.0 15 28511.0 16 32699.0 17 37097.0 18 36156.0 19 35190.0 20 36702.0 21 40345.0 22 44495.0 23 49547.0 24 55650.0 25 62476.0 26 71278.0 27 81051.0 28 93780.0 29 108297.0 30 125265.0 31 147685.0 32 175661.0 33 211306.0 34 259965.0 35 323171.0 36 384893.0 37 372046.0 38 171057.0 39 9669.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.663960680344616 17.970666907928155 14.409800757863723 23.9555716538635 2 15.891087496671435 19.41435167890492 39.401695156613286 25.29286566781035 3 19.458552186477405 22.93260605603939 29.128809369718912 28.480032387764297 4 13.355296915466287 15.843505965002805 35.67091793319669 35.13027918633422 5 14.44688512749083 36.72127388966218 33.61893476159593 15.212906221251057 6 33.8447939622333 34.974639859823874 16.6276809530546 14.552885224888223 7 29.963088227500645 31.904132828746874 19.751565446085888 18.381213497666597 8 27.424641860886812 34.02517907753759 19.30934395592959 19.24083510564601 9 26.77308621368919 16.54239807623666 19.366041822441392 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23.51029582794868 26.026505660570137 27.501502647293357 22.961695864187824 36-37 23.629231190681594 25.43262565779853 27.521569023108917 23.416574128410957 38-39 22.918433316750164 25.87719613259448 27.49524411014224 23.709126440513113 40-41 23.475954657061468 25.60900206464101 27.704430742594045 23.21061253570348 42-43 23.129861756153304 25.679440896047396 27.49488488191339 23.69581246588591 44-45 23.029603047541965 25.96108936253325 27.81909679564806 23.190210794276716 46-47 23.629735676175905 26.36513369711046 26.908852461527882 23.096278165185755 48-49 23.219567356503358 26.3808037707654 26.790390650707895 23.609238222023347 50-51 23.47374604304825 26.48765986388262 26.934113013270718 23.104481079798415 52-53 23.821692093463547 26.02184062966071 26.990948356064308 23.16551892081144 54-55 23.059334789563653 26.43953282326973 27.16315311738399 23.337979269782625 56-57 23.14034143415366 26.56902894359862 26.983354682077128 23.30727494017059 58-59 23.127095088409018 25.951456983085457 26.99555824501368 23.925889683491842 60-61 22.63019454038364 26.348191971449047 27.396702833567733 23.624910654599578 62-63 22.896306469801363 26.31033300286286 27.576269506893684 23.217091020442098 64-65 22.628464048449445 26.399183232652312 27.221743143392587 23.75060957550566 66-67 22.514985634694387 26.44598110529478 27.237114109434827 23.80191915057601 68-69 23.28890063377715 25.912925696347404 27.388121960451645 23.410051709423804 70-71 22.94976918544263 26.151886257037592 26.914155242979326 23.984189314540455 72-73 22.96934002802809 26.278608018025306 26.963063472402897 23.788988481543704 74-75 22.998202622045984 26.674161327359307 26.85718681463195 23.470449235962754 76-77 23.207144961353873 26.188946476942064 27.020818831261472 23.583089730442587 78-79 23.293854747890183 26.533129570833346 26.863475029333276 23.30954065194319 80-81 23.52464516499658 26.46441596739563 26.577363166415203 23.433575701192584 82-83 23.323120110973647 26.571346094834723 26.712185479054174 23.39334831513746 84-85 23.221774514623952 26.74617532340185 26.682106913978703 23.34994324799549 86-87 23.606259099848277 26.707867183864646 26.23741978888671 23.448453927400365 88-89 23.35499858982436 26.82709522811566 26.11209547631296 23.70581070574702 90-91 23.28689301380759 26.816806371785724 26.226119730689675 23.670180883717016 92-93 23.554991988049107 26.25000798003077 26.52600532427653 23.66899470764359 94-95 23.2506535104248 26.09143064553279 26.786593032318702 23.871322811723715 96-97 23.741926915771757 25.692300742609618 26.63220320396552 23.933569137653098 98-99 23.391218150702258 26.05809484913064 26.210623234141206 24.340063766025892 100-101 23.998656711720397 25.408630115954473 26.49934888542061 24.09336428690452 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 238.0 1 180.5 2 193.5 3 282.0 4 242.0 5 247.5 6 449.0 7 992.0 8 1727.0 9 1994.5 10 1704.0 11 1427.5 12 1467.5 13 1624.0 14 1834.0 15 2085.0 16 2288.0 17 2584.0 18 2884.0 19 3225.5 20 3795.0 21 4577.0 22 5456.5 23 6704.0 24 8573.5 25 11057.0 26 14280.0 27 18301.5 28 21861.0 29 25194.0 30 29407.5 31 36612.0 32 47868.5 33 52082.0 34 50443.0 35 52985.5 36 58876.5 37 65384.0 38 70144.5 39 74546.5 40 80188.0 41 86491.0 42 90702.5 43 94797.0 44 98794.0 45 105501.5 46 110131.5 47 112599.0 48 115401.5 49 114551.0 50 114380.0 51 113818.5 52 114417.5 53 119208.0 54 123929.5 55 119532.5 56 110420.0 57 102258.0 58 96786.0 59 87049.5 60 70143.5 61 58443.0 62 50429.0 63 39181.0 64 28338.5 65 21483.0 66 16334.0 67 12577.5 68 9727.0 69 7676.0 70 6176.0 71 4480.0 72 3217.0 73 2531.5 74 1891.5 75 1318.5 76 962.5 77 675.5 78 459.0 79 314.5 80 201.5 81 113.5 82 66.0 83 43.5 84 29.5 85 22.0 86 20.5 87 11.0 88 6.5 89 6.0 90 3.0 91 2.5 92 2.0 93 2.0 94 2.5 95 3.0 96 2.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1771470987894257 2 0.050166679750851595 3 0.04809235775097541 4 0.05434723639675589 5 0.06117654267327131 6 0.0658996143037586 7 0.038933428305368285 8 0.03548686252095863 9 0.04340119876664005 10-11 0.043879888458919165 12-13 0.041183269859080135 14-15 0.035614513105566396 16-17 0.03379549227490575 18-19 0.04201299865903061 20-21 0.05182613735075254 22-23 0.048842304935546026 24-25 0.06961743758045975 26-27 0.05441106168905977 28-29 0.0460339920741752 30-31 0.05525674681208622 32-33 0.05141127295077729 34-35 0.05046984988929503 36-37 0.048379571566342885 38-39 0.027428919367593476 40-41 0.02572159279846462 42-43 0.02182824996792779 44-45 0.004579464722803564 46-47 0.013036315953067985 48-49 0.01266932052232066 50-51 0.009653575460962217 52-53 0.009924832953253717 54-55 0.011169426153179425 56-57 0.0086323707841001 58-59 0.010626911168596424 60-61 0.011121557183951514 62-63 0.004547552076651622 64-65 0.007212258030338714 66-67 0.004356076199739976 68-69 0.008807890337935775 70-71 0.023487707567828735 72-73 0.01996136016803923 74-75 0.020966608521825376 76-77 0.012158718183889604 78-79 0.005345368230450153 80-81 0.007387777584174392 82-83 0.00630274761500839 84-85 0.005887883215033154 86-87 0.008297287999504716 88-89 0.030811659859699244 90-91 0.03773670407467049 92-93 0.02342388227552485 94-95 0.03346040949031036 96-97 0.03684314998241613 98-99 0.022163332752523173 100-101 0.12511352923868554 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3133554.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.4704153162671 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69439375017694 33.78138668734974 2 13.94771429531289 12.963121520316514 3 5.212915462357897 7.2673903963308595 4 2.589374098199488 4.813171590100591 5 1.4347679345349797 3.333713090015162 6 0.904736783095639 2.522609643741468 7 0.607420377305157 1.9758954043454013 8 0.41686182337337346 1.5497393649325635 9 0.30829490079474253 1.2893932871837144 >10 1.6811493411224638 14.320906691404781 >50 0.1274236224288771 4.090752939067401 >100 0.06605299773711647 5.752588842389585 >500 0.004274930548835442 1.3014649098383952 >1k 0.004274930548081446 4.097285042531481 >5k 3.447524635549553E-4 0.9405805904524112 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7558 0.2411957796163717 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5753 0.1835934533121178 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5627 0.1795724598969732 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5332 0.17015822928215057 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5085 0.1622758056826211 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4704 0.15011708749873148 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4309 0.13751159226871468 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4241 0.13534153233038268 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4182 0.13345868620741816 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3944 0.12586347642325615 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3915 0.12493800968484986 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3569 0.1138962341162782 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3323 0.10604572316290065 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3271 0.10438626556299971 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.382529230388243E-5 2 0.0 0.0 0.0 0.0 6.382529230388243E-5 3 0.0 0.0 0.0 0.0 6.382529230388243E-5 4 0.0 0.0 0.0 0.0 6.382529230388243E-5 5 0.0 0.0 0.0 0.0 6.382529230388243E-5 6 0.0 0.0 0.0 0.0 6.382529230388243E-5 7 0.0 0.0 0.0 0.0 6.382529230388243E-5 8 0.0 0.0 0.0 0.0 6.382529230388243E-5 9 0.0 0.0 0.0 0.0 6.382529230388243E-5 10-11 0.0 0.0 0.0 1.5956323075970607E-5 6.382529230388243E-5 12-13 0.0 0.0 0.0 3.191264615194121E-5 2.8721381536747095E-4 14-15 0.0 0.0 0.0 4.786896922791182E-5 2.8721381536747095E-4 16-17 0.0 0.0 0.0 9.573793845582364E-5 2.8721381536747095E-4 18-19 0.0 0.0 0.0 1.2765058460776485E-4 4.627333692031476E-4 20-21 0.0 0.0 0.0 1.9147587691164728E-4 4.946460153550888E-4 22-23 0.0 0.0 0.0 3.6699543074732394E-4 5.106023384310594E-4 24-25 0.0 0.0 0.0 5.425149845830006E-4 5.106023384310594E-4 26-27 0.0 0.0 0.0 0.0010690736460900307 5.106023384310594E-4 28-29 0.0 0.0 0.0 0.0032391335844220333 5.106023384310594E-4 30-31 0.0 0.0 0.0 0.012047023922357809 5.106023384310594E-4 32-33 0.0 0.0 0.0 0.032949807151879305 5.106023384310594E-4 34-35 0.0 0.0 0.0 0.06376146701157855 5.106023384310594E-4 36-37 0.0 0.0 0.0 0.11467809394700075 5.106023384310594E-4 38-39 0.0 0.0 0.0 0.19155565852702713 5.744276307349419E-4 40-41 0.0 0.0 0.0 0.27607630186044346 5.744276307349419E-4 42-43 0.0 0.0 0.0 0.36464985125515625 5.744276307349419E-4 44-45 0.0 0.0 0.0 0.47734936114073667 5.903839538109125E-4 46-47 0.0 0.0 0.0 0.6068668355483902 6.382529230388243E-4 48-49 0.0 0.0 0.0 0.7427827955095077 6.861218922667361E-4 50-51 0.0 0.0 0.0 0.896745356869548 7.33990861494648E-4 52-53 0.0 0.0 0.0 1.043352053291566 7.33990861494648E-4 54-55 0.0 0.0 0.0 1.2058991164664787 7.659035076465891E-4 56-57 0.0 0.0 0.0 1.3991301889164827 7.818598307225597E-4 58-59 0.0 0.0 0.0 1.598839528535331 7.978161537985304E-4 60-61 0.0 0.0 0.0 1.8208398514913098 8.297287999504716E-4 62-63 1.5956323075970607E-5 0.0 0.0 2.038452185601397 8.93554092254354E-4 64-65 3.191264615194121E-5 0.0 0.0 2.2672977711569677 8.93554092254354E-4 66-67 3.191264615194121E-5 0.0 0.0 2.5399753762022295 9.254667384062952E-4 68-69 3.191264615194121E-5 0.0 0.0 2.835965169261484 9.254667384062952E-4 70-71 3.191264615194121E-5 0.0 0.0 3.14422537476616 9.414230614822658E-4 72-73 3.191264615194121E-5 0.0 0.0 3.4698301034544166 9.73335707634207E-4 74-75 3.191264615194121E-5 0.0 0.0 3.797301721942561 0.0010052483537861482 76-77 3.191264615194121E-5 0.0 0.0 4.1567178992287985 0.00105311732301406 78-79 3.191264615194121E-5 1.5956323075970607E-5 0.0 4.549610442328423 0.0010690736460900307 80-81 3.191264615194121E-5 3.191264615194121E-5 0.0 4.9583954832117145 0.001100986292241972 82-83 3.191264615194121E-5 3.191264615194121E-5 0.0 5.384923955355484 0.0011488552614698838 84-85 3.191264615194121E-5 6.382529230388243E-5 0.0 5.825669511359944 0.0011648115845458544 86-87 3.191264615194121E-5 6.382529230388243E-5 0.0 6.278366353348307 0.0012126805537737663 88-89 4.786896922791182E-5 6.382529230388243E-5 0.0 6.757040089304349 0.0012445931999257073 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4185 0.0 56.751183 1 GTATCAA 7775 0.0 44.259624 1 ATCAACG 10490 0.0 32.709885 3 TCAACGC 10540 0.0 32.599808 4 TATCAAC 10520 0.0 32.526257 2 CAACGCA 10775 0.0 32.018578 5 AACGCAG 11245 0.0 30.716686 6 TGGTATC 1950 0.0 28.27097 2 TAAGGTG 2000 0.0 26.61158 5 ACGCAGA 12880 0.0 26.591908 7 CGCAGAG 13055 0.0 26.163092 8 GTAAGGT 2015 0.0 25.708029 4 AGGTAAG 1900 0.0 24.512625 2 AAGGTAA 1920 0.0 23.55007 1 GTACATG 10385 0.0 23.419857 1 TACATGG 10130 0.0 23.363464 2 GTGGTAT 2510 0.0 23.323902 1 ACATGGG 10195 0.0 22.375427 3 GCAGAGT 14690 0.0 22.344238 9 GGTAAGG 2320 0.0 21.508923 3 >>END_MODULE