FastQCFastQC Report
Thu 26 May 2016
SRR938426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938426_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3133554
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73890.23580254241669366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58530.18678471792731194No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA56030.17880655638932663No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA54240.17309419272812915No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA53350.17025396722060637No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC49790.1588930651905153No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48150.15365939122159694No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG45140.14405368472986266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42390.13527770703807881No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38470.12276794974651786No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA38210.12193822094656737No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36620.11686411020840873No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34390.10974759011652584No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA32710.10438626556299971No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32250.10291828384001041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42950.055.1403771
GTATCAA77350.042.975451
ATCAACG103800.031.6459223
TATCAAC106150.031.3150982
TCAACGC107600.030.5724934
CAACGCA110000.029.8622425
AACGCAG114500.028.7049226
AGGTAAG20600.027.701972
TAAGGTG23600.026.3873865
GTAAGGT23550.026.0396924
ACGCAGA132150.024.835147
AAGGTAA21900.024.7550771
CGCAGAG133500.024.4772688
TGGTATC20250.024.1884942
GGTAAGG24600.023.7687873
GTACATG100150.023.3624111
TACATGG97850.023.3279742
GTGTAAG13700.022.5629731
TAAGGTA10950.022.140794
ACATGGG100350.021.838393
Produced by FastQC (version 0.11.5)