##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938426_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3133554 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77639064142504 31.0 31.0 34.0 30.0 34.0 2 32.00882671879916 31.0 31.0 34.0 30.0 34.0 3 32.0750499273349 33.0 31.0 34.0 30.0 34.0 4 35.74850856248209 37.0 35.0 37.0 33.0 37.0 5 35.6081372779917 37.0 35.0 37.0 33.0 37.0 6 35.636664949766306 37.0 35.0 37.0 33.0 37.0 7 35.57537894671673 37.0 35.0 37.0 33.0 37.0 8 35.55880511393772 37.0 35.0 37.0 33.0 37.0 9 37.14450875906399 39.0 37.0 39.0 33.0 39.0 10-11 37.05237615180718 39.0 37.0 39.0 33.0 39.0 12-13 36.94945403844963 39.0 37.0 39.0 33.0 39.0 14-15 38.00849961417611 40.0 37.0 41.0 33.0 41.0 16-17 37.927713229132166 40.0 37.0 41.0 33.0 41.0 18-19 38.02821030050862 40.0 37.0 41.0 33.0 41.0 20-21 38.0706421526484 40.0 37.0 41.0 33.0 41.0 22-23 37.979297468625084 40.0 37.0 41.0 33.0 41.0 24-25 37.870538213159875 40.0 37.0 41.0 32.0 41.0 26-27 37.67778567083893 40.0 37.0 41.0 32.0 41.0 28-29 37.55512239457178 39.5 37.0 41.0 32.0 41.0 30-31 37.31688395987432 39.0 36.0 41.0 31.0 41.0 32-33 37.10424186083917 39.0 36.0 41.0 31.0 41.0 34-35 36.95004569890929 39.0 36.0 41.0 30.5 41.0 36-37 36.94764315534374 39.0 36.0 41.0 30.5 41.0 38-39 36.81508504401073 39.0 35.0 41.0 30.0 41.0 40-41 36.64012747187379 39.0 35.0 41.0 30.0 41.0 42-43 36.42374233857147 39.0 35.0 40.0 30.0 41.0 44-45 36.16257115722276 39.0 35.0 40.0 29.5 41.0 46-47 35.80609987892342 38.0 35.0 40.0 28.5 41.0 48-49 35.4410819152949 38.0 34.0 40.0 27.5 41.0 50-51 35.06592961219114 38.0 34.0 40.0 26.5 41.0 52-53 34.55995843696965 38.0 33.0 40.0 25.5 41.0 54-55 34.23397251172311 37.0 33.0 40.0 24.0 41.0 56-57 33.87821639582404 37.0 33.0 40.0 24.0 41.0 58-59 33.41581651377318 36.5 32.0 39.0 23.0 41.0 60-61 33.0285650095706 36.0 31.5 39.0 22.0 40.0 62-63 32.55985711431812 35.5 31.0 39.0 21.0 40.0 64-65 32.04184418076089 35.0 31.0 38.0 19.5 40.0 66-67 31.546766865993057 35.0 30.0 38.0 18.0 40.0 68-69 31.0026430053543 34.0 29.5 37.0 16.0 39.5 70-71 30.408903755926975 34.0 29.0 36.5 12.0 39.0 72-73 29.969157384873533 34.0 29.0 36.0 8.0 39.0 74-75 29.46342491624526 34.0 28.0 35.5 7.0 38.0 76-77 28.208361496243562 31.5 26.5 34.5 7.0 36.0 78-79 28.91045056188596 33.0 28.0 35.0 4.0 37.0 80-81 28.664631756784786 33.0 28.0 35.0 2.0 36.5 82-83 28.201747759891802 33.0 27.0 35.0 2.0 36.0 84-85 27.695037009095742 33.0 26.0 35.0 2.0 36.0 86-87 27.137491168175178 32.0 25.5 35.0 2.0 35.0 88-89 26.48491920037121 32.0 24.5 34.0 2.0 35.0 90-91 25.944451571602084 32.0 23.5 34.0 2.0 35.0 92-93 25.21120682777447 31.0 20.5 34.0 2.0 35.0 94-95 24.43412926664101 31.0 18.0 34.0 2.0 35.0 96-97 23.427069551059276 31.0 4.5 34.0 2.0 35.0 98-99 22.153492807208686 30.0 2.0 34.0 2.0 35.0 100-101 18.4465525406615 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33971545474883413 1101 2 0.3319713726071072 1101 3 0.26186768316425457 1101 4 0.21017242369050138 1101 5 0.13273356411018256 1101 6 0.12256856147251227 1101 7 0.3323283846552698 1101 8 0.26124081022870627 1101 9 0.32468549164040184 1101 10-11 0.36985504360941945 1101 12-13 0.42388491233725745 1101 14-15 0.4149049814532333 1101 16-17 0.5306112241116239 1101 18-19 0.5890184155534541 1101 20-21 0.4812005703902926 1101 22-23 0.5760472964078716 1101 24-25 0.5053012205247143 1101 26-27 0.6085201473042829 1101 28-29 0.5348650722920141 1101 30-31 0.4475950411502154 1101 32-33 0.44366296358423085 1101 34-35 0.20855091536403592 1101 36-37 0.34518485888088435 1101 38-39 0.46590437118504013 1101 40-41 0.5256461975935878 1101 42-43 0.5628793689445573 1101 44-45 0.6442040511919203 1101 46-47 0.6787045526129276 1101 48-49 0.7452128537444693 1101 50-51 0.7767453971573772 1101 52-53 0.7592692786821331 1101 54-55 0.7161998573439377 1101 56-57 0.5728062769385289 1101 58-59 0.7491504419434847 1101 60-61 0.8303554035668697 1101 62-63 0.6598965513488722 1101 64-65 0.6739496261278006 1101 66-67 0.5718947971557391 1101 68-69 0.576251292457254 1101 70-71 0.5096319990298603 1101 72-73 0.27514915469675216 1101 74-75 0.516260514528522 1101 76-77 0.613881298872716 1101 78-79 0.7004128171144366 1101 80-81 0.7897399673033725 1101 82-83 0.702234747854849 1101 84-85 0.7170949494871728 1101 86-87 0.7408921705755489 1101 88-89 0.62126272924516 1101 90-91 0.6326892030126956 1101 92-93 0.6356088218606111 1101 94-95 0.7439329566455868 1101 96-97 0.9214041727599742 1101 98-99 0.7010771104809912 1101 100-101 0.5175799340130887 1102 1 0.33741497249521757 1102 2 0.38641790758618555 1102 3 0.35283179875276005 1102 4 0.24992183851069427 1102 5 0.26845278939273953 1102 6 0.2278397667631964 1102 7 0.318409072138941 1102 8 0.3575306924523858 1102 9 0.4190341273653644 1102 10-11 0.4061562029172805 1102 12-13 0.3926620354146735 1102 14-15 0.5660338969807981 1102 16-17 0.5123997524950639 1102 18-19 0.5269412860917768 1102 20-21 0.5151401408846823 1102 22-23 0.4328503771656358 1102 24-25 0.5540607680893856 1102 26-27 0.4793958702957468 1102 28-29 0.41726155461429926 1102 30-31 0.41371357285454735 1102 32-33 0.4102145524889096 1102 34-35 0.4335106113226814 1102 36-37 0.49023648829079036 1102 38-39 0.4703398275823645 1102 40-41 0.5350859003335131 1102 42-43 0.6102147420245174 1102 44-45 0.547018118254627 1102 46-47 0.5448948134397611 1102 48-49 0.5303462017777179 1102 50-51 0.5395236694029251 1102 52-53 0.4798744750466355 1102 54-55 0.5548868549517181 1102 56-57 0.5963901364374351 1102 58-59 0.7926326681965605 1102 60-61 0.7216697954761244 1102 62-63 0.6175775493547278 1102 64-65 0.6298711414846494 1102 66-67 0.6956993162514102 1102 68-69 0.5776056691944831 1102 70-71 0.5699922970410292 1102 72-73 0.5001968891670217 1102 74-75 0.5176491195231634 1102 76-77 0.5532768428686374 1102 78-79 0.7241994709911062 1102 80-81 0.7490585030850454 1102 82-83 0.43551073007975916 1102 84-85 0.42591623667351897 1102 86-87 0.27580740735407616 1102 88-89 0.4827688154907115 1102 90-91 0.43605580428085844 1102 92-93 0.37441518917487926 1102 94-95 0.3466156902065016 1102 96-97 0.32428975434796214 1102 98-99 0.38198165924472605 1102 100-101 0.368857994612128 1103 1 0.27319254786397096 1103 2 0.2536050011885749 1103 3 0.30830025949328643 1103 4 0.2933833185196306 1103 5 0.2151602483921593 1103 6 0.12214859375753662 1103 7 0.2190065754360191 1103 8 0.25638576679349967 1103 9 0.2965442175008519 1103 10-11 0.33799186809103077 1103 12-13 0.34413672006287754 1103 14-15 0.44930915954871864 1103 16-17 0.44845639393086856 1103 18-19 0.3831831453898431 1103 20-21 0.4431703689620363 1103 22-23 0.4163546248653276 1103 24-25 0.48278284745798317 1103 26-27 0.5119863704217096 1103 28-29 0.4577667804731931 1103 30-31 0.617973818281726 1103 32-33 0.5286782509506835 1103 34-35 0.49283989398212924 1103 36-37 0.513397135895417 1103 38-39 0.5899741041576476 1103 40-41 0.5661791232915974 1103 42-43 0.3515197838793256 1103 44-45 0.5414295040685673 1103 46-47 0.569653665066582 1103 48-49 0.5233428266694631 1103 50-51 0.4338329043885878 1103 52-53 0.5101740528776162 1103 54-55 0.585926070058008 1103 56-57 0.5443401840194184 1103 58-59 0.6396053215579514 1103 60-61 0.5621303462848175 1103 62-63 0.47685593509624624 1103 64-65 0.35777596322813565 1103 66-67 0.547334341490771 1103 68-69 0.39450120199546035 1103 70-71 0.2567322219502266 1103 72-73 0.22958317841451148 1103 74-75 0.45487690006055104 1103 76-77 0.4062971006579552 1103 78-79 0.46804599281405146 1103 80-81 0.4114686302457429 1103 82-83 0.4307985832600032 1103 84-85 0.3102551836275076 1103 86-87 0.4601167239380075 1103 88-89 0.44535937681537874 1103 90-91 0.5201115032566967 1103 92-93 0.4747798314225875 1103 94-95 0.40189820890015326 1103 96-97 0.26293489675223114 1103 98-99 0.22517202901746103 1103 100-101 -0.07390954315002318 1104 1 0.2683606345471432 1104 2 0.2635224738981705 1104 3 0.2764760199329075 1104 4 0.20132825609346838 1104 5 0.16734277957265675 1104 6 0.18089357785454752 1104 7 0.1941781888758456 1104 8 0.2591277904685825 1104 9 0.20179797909486297 1104 10-11 0.31790663353110915 1104 12-13 0.3301322280886154 1104 14-15 0.44834468243975323 1104 16-17 0.42339697124079123 1104 18-19 0.40333576548766104 1104 20-21 0.4213470318248298 1104 22-23 0.4249766661465486 1104 24-25 0.5007674304367811 1104 26-27 0.517903807982222 1104 28-29 0.5989489607552372 1104 30-31 0.5742470712289673 1104 32-33 0.5003715364680943 1104 34-35 0.5424273562635094 1104 36-37 0.595726070919568 1104 38-39 0.5273377410059723 1104 40-41 0.586293780401391 1104 42-43 0.6946251227293558 1104 44-45 0.5570046467926133 1104 46-47 0.48262818020171494 1104 48-49 0.5027367734833703 1104 50-51 0.5980770815376175 1104 52-53 0.7768412856157099 1104 54-55 0.7193032772182306 1104 56-57 0.6079026169917938 1104 58-59 0.6118234244037808 1104 60-61 0.5174828610143578 1104 62-63 0.6926076427395671 1104 64-65 0.7888645501815503 1104 66-67 0.45105492165606265 1104 68-69 0.5018209400044356 1104 70-71 0.30364435496068864 1104 72-73 0.2360557444538216 1104 74-75 0.4434751683164002 1104 76-77 0.312318563716957 1104 78-79 0.18285311812627114 1104 80-81 0.16729730648967944 1104 82-83 0.18330512780267938 1104 84-85 0.28807897335856225 1104 86-87 0.2960255341750013 1104 88-89 0.3052566980403135 1104 90-91 0.2801440497309997 1104 92-93 0.5264501732750979 1104 94-95 0.5993752548682458 1104 96-97 0.5122406529285506 1104 98-99 0.2342687310655691 1104 100-101 -0.11445544598096546 1105 1 0.20053174457362388 1105 2 0.200978878830405 1105 3 0.14539589512178708 1105 4 0.15386522533142255 1105 5 0.009029167334624333 1105 6 0.17231283709230638 1105 7 0.1163814390930824 1105 8 0.13227842170750392 1105 9 0.15784183653251205 1105 10-11 0.2057953688471983 1105 12-13 0.26674692066815453 1105 14-15 0.34668119918154616 1105 16-17 0.2395283801274246 1105 18-19 0.2699484371938823 1105 20-21 0.28919489910662577 1105 22-23 0.2885329220763637 1105 24-25 0.37096316686182007 1105 26-27 0.27259608586920336 1105 28-29 0.29800574653187084 1105 30-31 0.4318727274042402 1105 32-33 0.38213221447347934 1105 34-35 0.3916784637584172 1105 36-37 0.3331812157890752 1105 38-39 0.2281342724733193 1105 40-41 0.2356511150021774 1105 42-43 0.37218915229157545 1105 44-45 0.3610062814376178 1105 46-47 0.2576442674797619 1105 48-49 0.0899624198836193 1105 50-51 -0.06286672071501442 1105 52-53 -0.11612607522777552 1105 54-55 0.00851588025102501 1105 56-57 0.016638755943873207 1105 58-59 0.15708296820952228 1105 60-61 0.07883665140234797 1105 62-63 0.11243973174505584 1105 64-65 -0.01364185776242266 1105 66-67 0.12880566960351914 1105 68-69 -0.09912511890598097 1105 70-71 0.0735688342469274 1105 72-73 0.20154205372915257 1105 74-75 0.14441927985715353 1105 76-77 0.09500189401309456 1105 78-79 0.11109583942645784 1105 80-81 0.3029614822096285 1105 82-83 0.16496959238245879 1105 84-85 0.1506487051818972 1105 86-87 0.207573378995221 1105 88-89 0.06065232002874765 1105 90-91 0.24712992910538745 1105 92-93 0.20306629201653337 1105 94-95 0.10298223214100943 1105 96-97 -0.0616068502714846 1105 98-99 -0.3461682796876353 1105 100-101 -0.6767543311404722 1106 1 0.1399292967250929 1106 2 0.1322854052816922 1106 3 0.18502396303882307 1106 4 0.05896989603395042 1106 5 0.018612795016238692 1106 6 -0.0024257493452992662 1106 7 0.056036242833705785 1106 8 0.06324637549617762 1106 9 -0.0055442719003906404 1106 10-11 0.012866268938601877 1106 12-13 -0.07399647185425806 1106 14-15 0.06710585239957823 1106 16-17 -0.10423165925855926 1106 18-19 0.1341495905307042 1106 20-21 0.13267714607993497 1106 22-23 0.1920963291654445 1106 24-25 0.22937812462399165 1106 26-27 0.1318536852954395 1106 28-29 0.22309284851364453 1106 30-31 0.18786440139901117 1106 32-33 0.21989854449834922 1106 34-35 0.15658686712628622 1106 36-37 -0.14559978599858425 1106 38-39 0.0021921388273327125 1106 40-41 0.21140606513008464 1106 42-43 0.3644491454022116 1106 44-45 0.253024997075606 1106 46-47 -0.09127343699758228 1106 48-49 0.07783823390147404 1106 50-51 0.009213883160867908 1106 52-53 -0.03185067921333484 1106 54-55 -0.16007206306228738 1106 56-57 0.018240986967398953 1106 58-59 0.032134589310970796 1106 60-61 -0.33510749833248354 1106 62-63 -0.2772931973099375 1106 64-65 -0.60357983009024 1106 66-67 -0.3627959877742306 1106 68-69 -0.9814328911886321 1106 70-71 -0.756523369251898 1106 72-73 -0.5194735575774523 1106 74-75 -0.27570597331200375 1106 76-77 -0.254634598916887 1106 78-79 -0.313453972632022 1106 80-81 -1.0037780508838843 1106 82-83 -0.6048007162923064 1106 84-85 -0.44610414154697153 1106 86-87 -0.1793097564459174 1106 88-89 -0.17537367351777888 1106 90-91 -0.104830156752449 1106 92-93 -0.44026659182056704 1106 94-95 -0.35786008012124526 1106 96-97 -0.1732135197973328 1106 98-99 -0.3195712353390121 1106 100-101 -0.5233625264892225 1107 1 0.18668852260126556 1107 2 0.19810026069710318 1107 3 0.23796133816284737 1107 4 0.18207896906967846 1107 5 0.07042894471498329 1107 6 0.17418587529062535 1107 7 0.10326015111301245 1107 8 0.24217064166581537 1107 9 0.33678153152736456 1107 10-11 0.2142782061010564 1107 12-13 0.2141200811899111 1107 14-15 0.42633957824699564 1107 16-17 0.39006664840664484 1107 18-19 0.36982900257192597 1107 20-21 0.39255093976314726 1107 22-23 0.3384239142864729 1107 24-25 0.3564009899920535 1107 26-27 0.4032294865782262 1107 28-29 0.5503134617556782 1107 30-31 0.44400179623848146 1107 32-33 0.44161950321728227 1107 34-35 0.4339136335081406 1107 36-37 0.349645228774321 1107 38-39 0.32668886980351175 1107 40-41 0.4427613568595419 1107 42-43 0.43035358749580865 1107 44-45 0.47979060309319976 1107 46-47 0.4496324883191676 1107 48-49 0.498864542172754 1107 50-51 0.5241451026806274 1107 52-53 0.6290249062034334 1107 54-55 0.6620568504790327 1107 56-57 0.6789793856523048 1107 58-59 0.4657064097141017 1107 60-61 0.49628877599351284 1107 62-63 0.5594913757089088 1107 64-65 0.5835204640899647 1107 66-67 0.530279870190494 1107 68-69 0.5137684940048786 1107 70-71 0.28171480809047367 1107 72-73 0.34740675407283206 1107 74-75 0.5431965419606293 1107 76-77 0.5318050904916234 1107 78-79 0.525689883583869 1107 80-81 0.5876173380719898 1107 82-83 0.6348297283916828 1107 84-85 0.6018736548306798 1107 86-87 0.7274904337717487 1107 88-89 0.7493689530376635 1107 90-91 0.6130707949747993 1107 92-93 0.5589139844367246 1107 94-95 0.41480603402578353 1107 96-97 0.5243984452953505 1107 98-99 0.5799629413169711 1107 100-101 0.17479800298036707 1108 1 0.10870399237326112 1108 2 0.14474929978453588 1108 3 0.10003570619617363 1108 4 0.0609007344911916 1108 5 0.059837582827370284 1108 6 0.021492132781972373 1108 7 0.07752852427299928 1108 8 -0.016674198226979797 1108 9 0.11202765173907636 1108 10-11 0.1603660955949877 1108 12-13 0.15286746062130874 1108 14-15 0.2024148530980625 1108 16-17 0.10171656288744657 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-0.807344382984887 2316 74-75 -0.3831652786951736 2316 76-77 -0.455026597328569 2316 78-79 -0.3900076392563214 2316 80-81 -0.6635764688902945 2316 82-83 -0.5406475041982297 2316 84-85 -0.5176985240363088 2316 86-87 -0.40470168084698344 2316 88-89 -0.22186995851918923 2316 90-91 -0.3586314612683097 2316 92-93 -0.44301811546036873 2316 94-95 -0.46553592623684636 2316 96-97 -0.2908920675391009 2316 98-99 -0.32737820916341676 2316 100-101 -0.2343480222233012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 18.0 4 44.0 5 93.0 6 238.0 7 613.0 8 1390.0 9 2503.0 10 4277.0 11 6254.0 12 8857.0 13 11961.0 14 15692.0 15 20599.0 16 25312.0 17 31082.0 18 29966.0 19 27224.0 20 26867.0 21 28285.0 22 31060.0 23 35042.0 24 40763.0 25 47390.0 26 57238.0 27 69382.0 28 82580.0 29 98726.0 30 116701.0 31 139970.0 32 168833.0 33 209707.0 34 266183.0 35 350836.0 36 457482.0 37 492115.0 38 219716.0 39 8546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36138569467754 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23.118988854189205 26.23436519683401 28.130550805890053 22.51609514308673 20-21 23.23586892072069 25.540520444198506 28.500801326544877 22.72280930853593 22-23 23.178553808231804 25.404460877329704 28.453778042439993 22.963207271998503 24-25 23.16902437747882 25.668491435267626 28.304177883983268 22.858306303270286 26-27 22.44490058863452 26.090343039544805 28.15666770977336 23.308088662047314 28-29 22.810952037207592 26.462157665066567 27.952845235792967 22.77404506193287 30-31 22.826860491314335 25.95201806000471 27.764161715419615 23.456959733261336 32-33 22.44655748712165 25.91104222234562 27.88871677335064 23.753683517182086 34-35 23.45123316802366 26.039169727026 27.602677828380823 22.90691927656951 36-37 23.59417012560357 25.479444317059613 27.640300560115616 23.286084997221202 38-39 22.889819256047044 25.84465074078229 27.66420167418217 23.601328328988494 40-41 23.436257332337604 25.609214330850392 27.81110174954841 23.143426587263594 42-43 23.126581775552182 25.703744020148434 27.54716824578947 23.622505958509908 44-45 23.020542014042526 25.967372186241594 27.896587691896755 23.11549810781913 46-47 23.64069679348114 26.346250934242715 26.954825096360235 23.05822717591591 48-49 23.18290988443154 26.364122016087805 26.883963065579852 23.5690050339008 50-51 23.433057161293537 26.488326034911157 27.010512663895376 23.068104139899933 52-53 23.878797046420775 26.01589122127782 27.005885330203338 23.099426402098064 54-55 23.116850706897026 26.41033152771581 27.186143911992584 23.28667385339458 56-57 23.12600963634263 26.535030192554526 27.005247077280302 23.333713093822542 58-59 23.115239118266352 26.043719048722313 26.944293923130097 23.896747909881242 60-61 22.59391413072824 26.406677529731414 27.365588721304945 23.633819618235396 62-63 22.849119562005313 26.396274007085886 27.47919774160586 23.275408689302946 64-65 22.555842343868974 26.440425153037094 27.151167651810056 23.852564851283876 66-67 22.421025724162227 26.49504460114273 27.194442600601487 23.88948707409355 68-69 23.238093019389158 25.97285265704376 27.277930525307003 23.511123798260076 70-71 22.877881098696893 26.189238813441058 26.83796022305064 24.094919864811413 72-73 22.839769156682795 26.296738463737977 26.872442600318998 23.991049779260226 74-75 22.84584851577474 26.640852527194365 26.719293811435836 23.794005145595065 76-77 22.979929498582123 26.167986892837973 27.031367578155667 23.82071603042424 78-79 23.025580538902474 26.421373303284383 27.00196007472665 23.55108608308649 80-81 23.27722978763669 26.39407452605639 26.64015549278982 23.688540193517102 82-83 23.08045680625912 26.522444195276364 26.751785561275216 23.6453134371893 84-85 22.938630567958185 26.633616395691785 26.692497961883998 23.735255074466032 86-87 23.260546026866844 26.625212363018175 26.282399771117866 23.831841838997118 88-89 22.978355865278722 26.75225327432041 26.117717567066883 24.15167329333398 90-91 22.84309675273935 26.660095648367864 26.395228400330435 24.101579198562355 92-93 22.95344202780033 26.219557632167113 26.647749729657367 24.17925061037519 94-95 22.710377871501734 25.944774492333302 26.85798735747803 24.486860278686933 96-97 23.057285752854426 25.532015723999013 26.833333652459796 24.577364870686765 98-99 22.63937580090833 25.96617607848475 26.398161957765815 24.99628616284111 100-101 22.70970916729056 25.522378104861126 26.88918078322569 24.878731944622622 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 268.0 1 189.0 2 167.5 3 275.5 4 274.5 5 255.5 6 446.0 7 1021.0 8 1621.5 9 1824.0 10 1634.0 11 1400.5 12 1449.5 13 1583.0 14 1740.0 15 1976.5 16 2265.5 17 2550.0 18 2806.0 19 3154.5 20 3720.0 21 4431.0 22 5454.5 23 6730.5 24 8310.0 25 10712.5 26 14013.0 27 18124.5 28 21880.5 29 25298.0 30 29587.5 31 36864.5 32 49249.0 33 53345.5 34 50705.5 35 53291.5 36 59164.0 37 65275.5 38 69645.0 39 74156.5 40 79977.0 41 86676.5 42 90710.0 43 94583.0 44 99184.0 45 106263.5 46 110551.0 47 113538.5 48 115735.5 49 113998.5 50 114029.0 51 114125.0 52 115331.5 53 120719.0 54 127209.5 55 121931.0 56 111516.0 57 103295.5 58 96972.5 59 86975.5 60 70002.0 61 58104.0 62 49633.0 63 37522.0 64 26415.0 65 19608.0 66 14711.5 67 11754.0 68 9258.0 69 7143.0 70 5432.5 71 3717.5 72 2751.5 73 2270.5 74 1669.0 75 1170.5 76 838.0 77 569.0 78 380.0 79 237.0 80 134.0 81 76.5 82 47.5 83 27.5 84 14.0 85 8.5 86 4.0 87 1.5 88 2.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.701655691907655E-4 2 0.05067728208928264 3 0.001212680553773766 4 0.0 5 0.0888128942408524 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.9147587691164728E-4 26-27 0.009111060476379218 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010961993953191806 36-37 1.4360690768373547E-4 38-39 0.004579464722803564 40-41 0.005552800430437771 42-43 3.3508278459538275E-4 44-45 1.5956323075970607E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.382529230388243E-5 68-69 7.818598307225597E-4 70-71 1.7551955383567669E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0010371609999380894 82-83 4.946460153550888E-4 84-85 0.004643290015107447 86-87 0.002026453030648267 88-89 9.573793845582364E-5 90-91 0.0031114829998142683 92-93 9.73335707634207E-4 94-95 6.382529230388243E-5 96-97 0.0 98-99 7.978161537985303E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3133554.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.1590272486779 #Duplication Level Percentage of deduplicated Percentage of total 1 70.53529719753692 31.14770110939627 2 14.842936145107924 13.10899243364414 3 5.782620520565695 7.660646914092735 4 2.80323407868009 4.951523602594262 5 1.5502375469745204 3.422849103938572 6 0.9748460402186324 2.5828951711968204 7 0.6514818564191602 2.013816353474307 8 0.4630846962188567 1.6359495775019377 9 0.340950067655809 1.3550420995246297 >10 1.827126205962586 14.88244593124784 >50 0.1429475031118679 4.360269416949704 >100 0.07462128319213296 6.034606726962998 >500 0.005777882098758027 1.667727667839325 >1k 0.004477858625989218 4.231367402682422 >5k 3.6111763112816274E-4 0.9441664889541237 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7389 0.23580254241669366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5853 0.18678471792731194 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5603 0.17880655638932663 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5424 0.17309419272812915 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5335 0.17025396722060637 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4979 0.1588930651905153 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4815 0.15365939122159694 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4514 0.14405368472986266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4239 0.13527770703807881 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3847 0.12276794974651786 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3821 0.12193822094656737 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3662 0.11686411020840873 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3439 0.10974759011652584 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3271 0.10438626556299971 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3225 0.10291828384001041 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.382529230388243E-5 2 0.0 0.0 0.0 0.0 6.382529230388243E-5 3 0.0 0.0 0.0 0.0 6.382529230388243E-5 4 0.0 0.0 0.0 6.382529230388243E-5 6.382529230388243E-5 5 0.0 6.382529230388243E-5 0.0 6.382529230388243E-5 6.382529230388243E-5 6 0.0 6.382529230388243E-5 0.0 6.382529230388243E-5 1.2765058460776485E-4 7 0.0 6.382529230388243E-5 0.0 6.382529230388243E-5 1.2765058460776485E-4 8 0.0 6.382529230388243E-5 0.0 9.573793845582364E-5 1.2765058460776485E-4 9 0.0 6.382529230388243E-5 0.0 9.573793845582364E-5 1.2765058460776485E-4 10-11 0.0 6.382529230388243E-5 0.0 9.573793845582364E-5 1.5956323075970607E-4 12-13 0.0 6.382529230388243E-5 0.0 9.573793845582364E-5 4.148643999752358E-4 14-15 0.0 6.382529230388243E-5 0.0 1.1169426153179425E-4 4.627333692031476E-4 16-17 0.0 6.382529230388243E-5 0.0 1.5956323075970607E-4 4.786896922791182E-4 18-19 0.0 6.382529230388243E-5 0.0 1.9147587691164728E-4 5.744276307349419E-4 20-21 0.0 6.382529230388243E-5 0.0 2.553011692155297E-4 5.903839538109125E-4 22-23 0.0 6.382529230388243E-5 0.0 4.46777046127177E-4 6.06340276886883E-4 24-25 0.0 6.382529230388243E-5 0.0 6.382529230388243E-4 6.06340276886883E-4 26-27 0.0 6.382529230388243E-5 0.0 0.0011648115845458544 6.382529230388243E-4 28-29 0.0 6.382529230388243E-5 0.0 0.003382740492105769 7.33990861494648E-4 30-31 0.0 6.382529230388243E-5 0.0 0.012318281414649308 7.33990861494648E-4 32-33 0.0 6.382529230388243E-5 0.0 0.033237020967246775 7.978161537985304E-4 34-35 0.0 6.382529230388243E-5 0.0 0.06419228773462975 8.297287999504716E-4 36-37 0.0 6.382529230388243E-5 0.0 0.11515678363927988 8.297287999504716E-4 38-39 0.0 6.382529230388243E-5 0.0 0.19225773674236984 8.616414461024128E-4 40-41 0.0 6.382529230388243E-5 0.0 0.27741663299882496 8.93554092254354E-4 42-43 0.0 6.382529230388243E-5 0.0 0.3662773962089053 8.93554092254354E-4 44-45 0.0 6.382529230388243E-5 0.0 0.4795832463713726 9.73335707634207E-4 46-47 0.0 6.382529230388243E-5 0.0 0.6094517598866973 0.00105311732301406 48-49 0.0 6.382529230388243E-5 0.0 0.7457347152785623 0.00105311732301406 50-51 0.0 6.382529230388243E-5 0.0 0.8993941065001592 0.0010850299691660013 52-53 0.0 6.382529230388243E-5 0.0 1.0460327155683293 0.001180767907621825 54-55 0.0 6.382529230388243E-5 0.0 1.2085319097740137 0.001180767907621825 56-57 0.0 6.382529230388243E-5 0.0 1.4013640741471187 0.0011967242306977956 58-59 0.0 6.382529230388243E-5 0.0 1.6010734137659668 0.0012445931999257073 60-61 0.0 6.382529230388243E-5 0.0 1.8233290378911613 0.0012445931999257073 62-63 0.0 6.382529230388243E-5 0.0 2.0401595121705256 0.0012445931999257073 64-65 0.0 6.382529230388243E-5 0.0 2.267648810264639 0.0012765058460776485 66-67 0.0 6.382529230388243E-5 0.0 2.540741279709876 0.0012765058460776485 68-69 0.0 6.382529230388243E-5 0.0 2.8380075786152084 0.0012765058460776485 70-71 0.0 6.382529230388243E-5 0.0 3.1466666921967836 0.0013084184922295898 72-73 0.0 6.382529230388243E-5 0.0 3.4741223543618522 0.0014201127537613839 74-75 0.0 6.382529230388243E-5 0.0 3.805535184649762 0.0015477633383691488 76-77 0.0 6.382529230388243E-5 0.0 4.1710785899971725 0.0015637196614451195 78-79 0.0 6.382529230388243E-5 0.0 4.570593007173324 0.0015956323075970607 80-81 0.0 6.382529230388243E-5 0.0 4.9862392669792825 0.001627544953749002 82-83 0.0 6.382529230388243E-5 0.0 5.42077781330719 0.0016754139229769138 84-85 0.0 6.382529230388243E-5 0.0 5.871990717249488 0.0016913702460528844 86-87 0.0 6.382529230388243E-5 0.0 6.337005840652498 0.001755195538356767 88-89 0.0 6.382529230388243E-5 0.0 6.829816878853851 0.0018190208306606492 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4295 0.0 55.140377 1 GTATCAA 7735 0.0 42.97545 1 ATCAACG 10380 0.0 31.645922 3 TATCAAC 10615 0.0 31.315098 2 TCAACGC 10760 0.0 30.572493 4 CAACGCA 11000 0.0 29.862242 5 AACGCAG 11450 0.0 28.704922 6 AGGTAAG 2060 0.0 27.70197 2 TAAGGTG 2360 0.0 26.387386 5 GTAAGGT 2355 0.0 26.039692 4 ACGCAGA 13215 0.0 24.83514 7 AAGGTAA 2190 0.0 24.755077 1 CGCAGAG 13350 0.0 24.477268 8 TGGTATC 2025 0.0 24.188494 2 GGTAAGG 2460 0.0 23.768787 3 GTACATG 10015 0.0 23.362411 1 TACATGG 9785 0.0 23.327974 2 GTGTAAG 1370 0.0 22.562973 1 TAAGGTA 1095 0.0 22.14079 4 ACATGGG 10035 0.0 21.83839 3 >>END_MODULE