FastQCFastQC Report
Thu 26 May 2016
SRR938425_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938425_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145561
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77750.24717371559476992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59150.18804276884155163No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA54590.17354614963753684No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54510.1732918229848348No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA51280.16302338438199102No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48620.15456702317964902No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC43230.13743176495385084No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA42840.13619192252192852No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG42120.13390298264761039No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA41080.1305967361624842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40390.12840316878292934No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36910.11733995939039173No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34010.10812061822994372No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT33200.10554556087133582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42350.053.6726341
GTATCAA81800.046.371131
TATCAAC107600.034.979282
ATCAACG108950.034.502233
TCAACGC110650.033.9291954
CAACGCA112600.033.2994085
AACGCAG118400.031.8648196
TGGTATC19200.029.9489822
ACGCAGA134000.027.9016387
CGCAGAG136850.027.285858
TAAGGTG22000.025.9212845
AGGTAAG19950.025.488182
GTGGTAT24550.025.039531
GTAAGGT23250.024.5276664
GCAGAGT154000.023.5677769
GTACATG106600.023.2899461
TACATGG103100.023.1850092
GGTAAGG24050.023.118983
AAGGTAA22400.022.124491
ACATGGG105400.022.0027583
Produced by FastQC (version 0.11.5)