FastQCFastQC Report
Thu 26 May 2016
SRR938425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938425_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145561
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75480.23995719682435024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59720.18985484624205348No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA55790.17736104942806705No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA55050.17500852789057342No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA52660.16741051914110075No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC50810.16152921529736666No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA46780.14871751016750273No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46230.14696901443017638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42460.13498387092159395No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA41190.13094643530994948No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38180.1213773950020362No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36300.11540071866353886No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33740.10726226577707441No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA33060.1051004892291073No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32350.10284334018637693No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG31930.10150812525969136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42450.053.4435771
GTATCAA79000.044.069571
ATCAACG105200.032.988423
TATCAAC107200.032.3879052
TCAACGC107250.032.3578724
CAACGCA109050.031.9109545
AACGCAG114850.030.3123666
ACGCAGA131050.026.4202797
TAAGGTG21250.026.398395
CGCAGAG134350.025.7006238
TGGTATC21900.025.192352
AGGTAAG20050.024.6702632
GTAAGGT22100.024.5226234
TACATGG99700.024.090792
GTACATG103300.023.7116451
GTGGTAT25700.023.5031131
GTGTAAG13600.023.0812761
GGTAAGG22800.022.7272053
AAGGTAA20600.022.6263051
ACATGGG102150.022.4782833
Produced by FastQC (version 0.11.5)