##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938425_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3145561 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.787680480524777 31.0 31.0 34.0 30.0 34.0 2 32.02607992660133 31.0 31.0 34.0 30.0 34.0 3 32.093676771806365 33.0 31.0 34.0 30.0 34.0 4 35.76866193343572 37.0 35.0 37.0 35.0 37.0 5 35.62834101770718 37.0 35.0 37.0 33.0 37.0 6 35.6681383702303 37.0 35.0 37.0 33.0 37.0 7 35.60208973852359 37.0 35.0 37.0 33.0 37.0 8 35.5812845466993 37.0 35.0 37.0 33.0 37.0 9 37.15585391604232 39.0 37.0 39.0 33.0 39.0 10-11 37.085749251087485 39.0 37.0 39.0 33.0 39.0 12-13 36.982592612255814 39.0 37.0 39.0 33.0 39.0 14-15 38.041211090803834 40.0 37.0 41.0 33.0 41.0 16-17 37.956079694528256 40.0 37.0 41.0 33.0 41.0 18-19 37.944832575174985 40.0 37.0 41.0 33.0 41.0 20-21 37.928803637888436 40.0 37.0 41.0 32.5 41.0 22-23 37.96308957289335 40.0 37.0 41.0 33.0 41.0 24-25 37.877919868665714 40.0 37.0 41.0 32.0 41.0 26-27 37.69730566344128 40.0 37.0 41.0 32.0 41.0 28-29 37.569176213718315 39.5 37.0 41.0 32.0 41.0 30-31 37.32267153617431 39.0 36.0 41.0 31.5 41.0 32-33 37.10263161324801 39.0 36.0 41.0 31.0 41.0 34-35 36.936498608674256 39.0 36.0 40.5 30.5 41.0 36-37 36.939651464396974 39.0 36.0 41.0 30.5 41.0 38-39 36.78164721650606 39.0 35.0 41.0 30.0 41.0 40-41 36.60307191626549 39.0 35.0 40.5 30.0 41.0 42-43 36.36622608812864 39.0 35.0 40.0 30.0 41.0 44-45 36.093355048590695 38.0 35.0 40.0 29.5 41.0 46-47 35.74219479450565 38.0 35.0 40.0 28.5 41.0 48-49 35.33422209901509 38.0 34.0 40.0 27.0 41.0 50-51 34.953538494405294 38.0 34.0 40.0 26.0 41.0 52-53 34.421166526416116 38.0 33.0 40.0 24.5 41.0 54-55 34.08062695334791 37.0 33.0 40.0 24.0 41.0 56-57 33.697055310642526 37.0 32.5 40.0 23.0 41.0 58-59 33.214796343164224 36.0 32.0 39.0 22.5 40.5 60-61 32.78439696448424 36.0 31.0 39.0 21.5 40.0 62-63 32.29201484250345 35.0 31.0 39.0 19.5 40.0 64-65 31.770315533540757 35.0 30.5 38.0 18.0 40.0 66-67 31.2673680465901 35.0 30.0 38.0 16.0 40.0 68-69 30.68567228548421 34.0 29.0 37.0 12.0 39.0 70-71 30.10121564960908 34.0 29.0 36.5 8.0 39.0 72-73 29.51965833757476 34.0 28.0 36.0 7.0 39.0 74-75 28.910793654931503 33.0 27.0 35.5 4.0 38.0 76-77 27.65082937510988 31.5 26.0 34.0 2.0 36.0 78-79 28.271747551549627 33.0 27.0 35.0 2.0 37.0 80-81 27.984878849909443 33.0 26.0 35.0 2.0 36.0 82-83 27.49353978511305 33.0 26.0 35.0 2.0 36.0 84-85 26.913086091797297 32.0 25.0 35.0 2.0 35.5 86-87 26.274459786346537 32.0 24.5 34.5 2.0 35.0 88-89 25.50456055374542 31.0 21.0 34.0 2.0 35.0 90-91 24.95352800343087 31.0 19.5 34.0 2.0 35.0 92-93 24.17189159580755 31.0 15.0 34.0 2.0 35.0 94-95 23.366119111980343 30.5 4.5 34.0 2.0 35.0 96-97 22.367624884718495 30.0 2.0 34.0 2.0 35.0 98-99 21.164280234908812 29.0 2.0 34.0 2.0 35.0 100-101 18.009316939013424 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2760126487077592 1101 2 0.3118155289487987 1101 3 0.261479158417103 1101 4 0.2092157805988748 1101 5 0.2084161822332149 1101 6 0.25808813171220635 1101 7 0.3039942145900838 1101 8 0.2821625736900657 1101 9 0.38485357390434416 1101 10-11 0.43390864153326447 1101 12-13 0.39795467011767016 1101 14-15 0.582690277974244 1101 16-17 0.5516581649124248 1101 18-19 0.6768600731502161 1101 20-21 0.6368594242687706 1101 22-23 0.6192191473890745 1101 24-25 0.5419408929240603 1101 26-27 0.5361125664066577 1101 28-29 0.5322434980805681 1101 30-31 0.5387843878366425 1101 32-33 0.4842487195205649 1101 34-35 0.3663276224160228 1101 36-37 0.4688615143713619 1101 38-39 0.6511496956424381 1101 40-41 0.7516164541510406 1101 42-43 0.6334511992931837 1101 44-45 0.6949001504205725 1101 46-47 0.8058716463847801 1101 48-49 0.8517066773366935 1101 50-51 1.0733934933476803 1101 52-53 1.0426936105039815 1101 54-55 1.0155430515338608 1101 56-57 1.069496416187853 1101 58-59 1.1606325553525494 1101 60-61 1.0999034768746228 1101 62-63 1.2332358577006985 1101 64-65 1.2764466072849423 1101 66-67 1.3181364373719688 1101 68-69 1.431312367631552 1101 70-71 1.6397754890213285 1101 72-73 1.6503672614083875 1101 74-75 1.65422431310704 1101 76-77 1.4833683089839091 1101 78-79 1.672218606753514 1101 80-81 2.082467487132252 1101 82-83 2.374315800252493 1101 84-85 2.3990463298415925 1101 86-87 2.443102542450255 1101 88-89 2.72039281936965 1101 90-91 2.916234991678216 1101 92-93 3.176000764349542 1101 94-95 3.260200012553664 1101 96-97 3.550080304314587 1101 98-99 3.796750153895225 1101 100-101 4.351223439132536 1102 1 0.36517091052153106 1102 2 0.39691753567267796 1102 3 0.3470718832599644 1102 4 0.23143069425336904 1102 5 0.20150540940437622 1102 6 0.22596175613534797 1102 7 0.2364359026604106 1102 8 0.26095102197673015 1102 9 0.363702578895051 1102 10-11 0.43314698436773824 1102 12-13 0.3735889493884841 1102 14-15 0.5968093867466067 1102 16-17 0.45768721124873935 1102 18-19 0.49211931848838475 1102 20-21 0.547522624586172 1102 22-23 0.569199309848095 1102 24-25 0.5192806786786193 1102 26-27 0.45141461907010694 1102 28-29 0.42575140177452653 1102 30-31 0.49811429658394957 1102 32-33 0.373588129405924 1102 34-35 0.5580684188410885 1102 36-37 0.703827268511148 1102 38-39 0.7915572004974223 1102 40-41 0.7527456278652593 1102 42-43 0.8313244060838869 1102 44-45 0.7055894527655795 1102 46-47 0.5780907363636842 1102 48-49 0.7482079615564601 1102 50-51 1.0037919146970538 1102 52-53 1.0694398361507993 1102 54-55 0.886691436340385 1102 56-57 1.1378383109862398 1102 58-59 1.1761554311695406 1102 60-61 1.1894323874804016 1102 62-63 1.4136394994555914 1102 64-65 1.535078025773526 1102 66-67 1.433376889250269 1102 68-69 1.547586459803039 1102 70-71 1.7363905780814441 1102 72-73 1.8796359870029917 1102 74-75 1.9874255701806867 1102 76-77 1.8040818340105318 1102 78-79 2.2676121209216937 1102 80-81 2.2971519658225574 1102 82-83 2.393224109049921 1102 84-85 2.5584467140634395 1102 86-87 2.7350114359330995 1102 88-89 2.895770254688827 1102 90-91 3.0784951140445216 1102 92-93 3.282421341099944 1102 94-95 3.4373904947103178 1102 96-97 3.787982014519436 1102 98-99 4.122013784374037 1102 100-101 4.585150854614174 1103 1 0.26460807416742327 1103 2 0.2916790154937132 1103 3 0.3182986005250541 1103 4 0.17931584477452844 1103 5 0.15316686293129322 1103 6 0.22782650688432682 1103 7 0.2817925534998409 1103 8 0.28214388175189953 1103 9 0.34275621382907673 1103 10-11 0.39967918553097803 1103 12-13 0.38563668286557373 1103 14-15 0.6302129731024948 1103 16-17 0.5078761939098513 1103 18-19 0.5019758074006972 1103 20-21 0.5485630698197781 1103 22-23 0.5437395434089396 1103 24-25 0.47474945009334846 1103 26-27 0.4212362758840982 1103 28-29 0.3809571812023691 1103 30-31 0.4657562315471111 1103 32-33 0.5515214722822961 1103 34-35 0.6100787517206214 1103 36-37 0.7189819216063924 1103 38-39 0.7463781111268517 1103 40-41 0.8185349946845122 1103 42-43 0.8390204622700637 1103 44-45 0.8521828410627705 1103 46-47 0.8572560959383466 1103 48-49 1.0451551530091407 1103 50-51 1.1441583674574787 1103 52-53 1.225290481473543 1103 54-55 1.2665890212404562 1103 56-57 1.3096533973152233 1103 58-59 1.5435529526185405 1103 60-61 1.5410724706875882 1103 62-63 1.3776510181086863 1103 64-65 1.5026707734687328 1103 66-67 1.5567542931574323 1103 68-69 1.6504466816685799 1103 70-71 1.6880788841003636 1103 72-73 1.7145924494181308 1103 74-75 1.849235590576395 1103 76-77 1.7548967487678269 1103 78-79 1.9820652082476222 1103 80-81 2.176884984032508 1103 82-83 2.3282171192802643 1103 84-85 2.5031378393402157 1103 86-87 2.674303281404878 1103 88-89 3.0256564691574965 1103 90-91 3.2405259441570777 1103 92-93 3.298634053445287 1103 94-95 3.4197816558242558 1103 96-97 3.610219278874858 1103 98-99 4.06870321220784 1103 100-101 4.511313658887428 1104 1 0.3474891951274266 1104 2 0.28178447416961205 1104 3 0.19426542209384223 1104 4 0.20287302250115857 1104 5 0.1433943355571543 1104 6 0.19958598315480458 1104 7 0.2166725087438408 1104 8 0.23525541303575181 1104 9 0.2092676857737672 1104 10-11 0.30993236594014917 1104 12-13 0.3926735521456166 1104 14-15 0.4634724250872253 1104 16-17 0.524408498931848 1104 18-19 0.5250130359928065 1104 20-21 0.5070269756355472 1104 22-23 0.5072955565899591 1104 24-25 0.4571279438033429 1104 26-27 0.5198286679485591 1104 28-29 0.44992125058120536 1104 30-31 0.49392828701693503 1104 32-33 0.5057045025704312 1104 34-35 0.46578145551463024 1104 36-37 0.5813962704469091 1104 38-39 0.6593060889548354 1104 40-41 0.7448015550790785 1104 42-43 0.7176963153874993 1104 44-45 0.7169670987468635 1104 46-47 0.8234374572394998 1104 48-49 0.8561428164332199 1104 50-51 0.860765753485623 1104 52-53 1.0664360724979929 1104 54-55 1.1219733768868352 1104 56-57 1.175697441717766 1104 58-59 1.3329387338092005 1104 60-61 1.2871421292416514 1104 62-63 1.361681674333063 1104 64-65 1.3976306610169296 1104 66-67 1.608455290511749 1104 68-69 1.6793028345365464 1104 70-71 1.6869877809362528 1104 72-73 1.8797411103954964 1104 74-75 2.0047379808208987 1104 76-77 1.9384020720287225 1104 78-79 2.2413985439217505 1104 80-81 2.3777957289262055 1104 82-83 2.524044973689822 1104 84-85 2.648400859867234 1104 86-87 2.8442409167686726 1104 88-89 2.8529299226087375 1104 90-91 3.110355347002667 1104 92-93 3.378316331460365 1104 94-95 3.716312568073107 1104 96-97 4.019111286873354 1104 98-99 4.371962301369315 1104 100-101 4.591121608344974 1105 1 0.2750200938660079 1105 2 0.23384334760117298 1105 3 0.2611276368851634 1105 4 0.2094516569302911 1105 5 0.16125547129437479 1105 6 0.19126554341310253 1105 7 0.27848328213086404 1105 8 0.2803082627161402 1105 9 0.2783941912902037 1105 10-11 0.37142843081973353 1105 12-13 0.37115389489604667 1105 14-15 0.48295626193544905 1105 16-17 0.47235397018185665 1105 18-19 0.4873498903909166 1105 20-21 0.4407159923179975 1105 22-23 0.343630652890198 1105 24-25 0.3032022431017154 1105 26-27 0.43600449796065277 1105 28-29 0.5262040271773003 1105 30-31 0.479838035246388 1105 32-33 0.5009437990166745 1105 34-35 0.5599100116458686 1105 36-37 0.5466900364655842 1105 38-39 0.5751872484304883 1105 40-41 0.41293642448206924 1105 42-43 0.5748998348791972 1105 44-45 0.5236142555460503 1105 46-47 0.640448365012027 1105 48-49 0.6403307785940413 1105 50-51 0.6035122888829818 1105 52-53 0.7296500282144507 1105 54-55 0.6198156432225517 1105 56-57 0.6264057184485168 1105 58-59 0.9576685489242749 1105 60-61 0.9648958801812739 1105 62-63 1.082737387008784 1105 64-65 1.0937243990198944 1105 66-67 1.225938101865303 1105 68-69 1.2140216156586128 1105 70-71 1.2272214049512549 1105 72-73 1.4114071909046935 1105 74-75 1.5427083440999532 1105 76-77 1.5206369687357864 1105 78-79 1.8309992676857725 1105 80-81 2.1387596826763264 1105 82-83 2.4119515338219024 1105 84-85 2.527941379828235 1105 86-87 2.6255618572723805 1105 88-89 2.766239760952871 1105 90-91 2.785487026435579 1105 92-93 3.0501270449207496 1105 94-95 3.0558644141695055 1105 96-97 3.438862022004713 1105 98-99 3.888070582819285 1105 100-101 4.3306535475598835 1106 1 0.13546614898336884 1106 2 0.17781739463173807 1106 3 0.10625706340053398 1106 4 0.0927114525778876 1106 5 0.029469021039417953 1106 6 0.1415501850537737 1106 7 0.15711226749552054 1106 8 0.0715645253882613 1106 9 0.1696549210728051 1106 10-11 0.19927796195219827 1106 12-13 0.05298201933353397 1106 14-15 0.0348776095024661 1106 16-17 0.0454515889857845 1106 18-19 0.2569972129613447 1106 20-21 0.38169820624433726 1106 22-23 0.2156576919140889 1106 24-25 0.17041088147555428 1106 26-27 0.11775557887887089 1106 28-29 0.2418481307893856 1106 30-31 0.14854590752020158 1106 32-33 0.213214810127333 1106 34-35 0.23728096822762268 1106 36-37 0.3670868440460211 1106 38-39 0.37344839269309205 1106 40-41 0.3327523097907985 1106 42-43 0.5050507195461336 1106 44-45 0.08555719791674221 1106 46-47 -0.15604812924868128 1106 48-49 0.3690030119966039 1106 50-51 0.2494252220725386 1106 52-53 0.3756680421672911 1106 54-55 0.4865939216811128 1106 56-57 0.3054339824407961 1106 58-59 0.7769277512189703 1106 60-61 0.6190588313240895 1106 62-63 0.8247797103359815 1106 64-65 0.4048564760208144 1106 66-67 0.37298600154116457 1106 68-69 0.36329308083126577 1106 70-71 0.6361443733065819 1106 72-73 0.9695259474345441 1106 74-75 1.3111898827763646 1106 76-77 1.2369982132162214 1106 78-79 1.4568227851564153 1106 80-81 1.3950843194691416 1106 82-83 1.7398279241629169 1106 84-85 1.7526515501193884 1106 86-87 1.898448965851248 1106 88-89 2.241732476641271 1106 90-91 2.334178106520689 1106 92-93 2.170367522483133 1106 94-95 2.7714705850396832 1106 96-97 3.2832323695892605 1106 98-99 3.359221154034543 1106 100-101 3.7826353613311348 1107 1 0.19719952008077613 1107 2 0.22137065901770114 1107 3 0.20949237214140481 1107 4 0.12261210959460556 1107 5 0.16800145093984753 1107 6 0.0769750113585772 1107 7 0.14911589195300934 1107 8 0.19789848830907886 1107 9 0.17153111411873567 1107 10-11 0.2082905039828873 1107 12-13 0.2511351828290813 1107 14-15 0.4101583960231423 1107 16-17 0.3597297057650479 1107 18-19 0.3678763952908852 1107 20-21 0.3730299262248238 1107 22-23 0.2661922826347336 1107 24-25 0.2299714215912232 1107 26-27 0.3042388886655232 1107 28-29 0.27878346427801404 1107 30-31 0.3072002773992111 1107 32-33 0.32116057286949484 1107 34-35 0.43584179939482226 1107 36-37 0.5100276432701847 1107 38-39 0.5145729130306691 1107 40-41 0.5250722921824647 1107 42-43 0.6365448418134818 1107 44-45 0.762949057747214 1107 46-47 0.6703287372380018 1107 48-49 0.7442393866229438 1107 50-51 0.7590989147567555 1107 52-53 0.8905909973068091 1107 54-55 0.9231430113490049 1107 56-57 1.01287803043693 1107 58-59 0.9381506089874989 1107 60-61 0.8857046327800262 1107 62-63 1.1652868215461538 1107 64-65 1.2711646632837095 1107 66-67 1.3669923510454538 1107 68-69 1.458815942901328 1107 70-71 1.449090691895723 1107 72-73 1.4042752381011745 1107 74-75 1.4447321196051668 1107 76-77 1.4845738544932487 1107 78-79 1.7610288988028238 1107 80-81 2.0392844333200095 1107 82-83 2.19875055305657 1107 84-85 2.418714745731158 1107 86-87 2.5613928543556064 1107 88-89 2.774716827453897 1107 90-91 2.909404453555794 1107 92-93 3.0903677347663994 1107 94-95 3.225098841283465 1107 96-97 3.5285533390981207 1107 98-99 3.696377262122482 1107 100-101 3.8884564758071214 1108 1 0.20592717862228582 1108 2 0.15553096384070386 1108 3 0.18292209456591735 1108 4 0.14302674088042266 1108 5 0.17385107413868894 1108 6 0.12772111310989231 1108 7 0.14406460171929325 1108 8 0.20554115589217758 1108 9 0.22975050703657018 1108 10-11 0.21443905882838976 1108 12-13 0.2730677922896163 1108 14-15 0.44752185162933955 1108 16-17 0.42306339367059564 1108 18-19 0.33482890170727586 1108 20-21 0.3229328279892343 1108 22-23 0.3579572067153691 1108 24-25 0.3621897617023393 1108 26-27 0.37030060059468894 1108 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0.7575056942324068 2316 78-79 0.8933151935363739 2316 80-81 1.061365779592638 2316 82-83 1.049263196114925 2316 84-85 1.2268236420960328 2316 86-87 1.2733350248069186 2316 88-89 1.517370641837669 2316 90-91 1.692342006183921 2316 92-93 1.6032157244076402 2316 94-95 2.3029373829054123 2316 96-97 2.6933381297619547 2316 98-99 2.7809288776164287 2316 100-101 3.1683472497204015 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 12.0 4 48.0 5 101.0 6 210.0 7 589.0 8 1439.0 9 2607.0 10 4128.0 11 6261.0 12 9242.0 13 12429.0 14 16355.0 15 21293.0 16 26440.0 17 32782.0 18 32275.0 19 29669.0 20 30172.0 21 32053.0 22 35338.0 23 39706.0 24 45780.0 25 53558.0 26 62784.0 27 75527.0 28 90089.0 29 106613.0 30 126205.0 31 148614.0 32 178227.0 33 215652.0 34 268670.0 35 339140.0 36 423963.0 37 446715.0 38 219482.0 39 11387.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.455565103414116 18.74494723827926 14.965266882781014 23.834220775525605 2 16.322113626171614 20.134661929857483 38.36637331950881 25.1768511244621 3 19.507101603944864 23.176435842682235 29.632176431709865 27.684286121663032 4 13.804087728707215 16.516990133079602 35.28493009672996 34.393992041483216 5 14.361191254055147 36.870167157707954 33.73800481303198 15.030636775204927 6 33.96065121611058 35.35251740468552 16.335082994734485 14.351748384469415 7 29.86065124790141 32.01839036025688 19.70720008291049 18.41375830893122 8 27.35330836057543 34.35810019262065 19.10762499916549 19.180966447638433 9 26.580505035508768 16.455156965641425 19.32958858531117 37.63474941353863 10-11 25.563293797195474 25.98018286722146 26.78552410841818 21.670999227164884 12-13 26.530037090363212 23.062420344097603 27.009236190301188 23.398306375237993 14-15 22.86867112098605 24.740928565683515 25.02841305573155 27.361987257598884 16-17 22.91009457454489 27.856795655846444 26.145129596914508 23.087980172694156 18-19 23.137605660802635 26.231521181754225 28.08632545991001 22.544547697533126 20-21 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25.980053823520528 27.890359612966602 23.102100961225126 46-47 23.631614661874938 26.34323083063601 26.955411271031434 23.069743236457615 48-49 23.194797366829 26.380715554395547 26.85786414569611 23.566622933079344 50-51 23.465607565709266 26.506432397909307 26.96576222810494 23.062197808276487 52-53 23.872021556727084 26.020970504148544 26.994485244444473 23.1125226946799 54-55 23.106148632946557 26.414191300057443 27.161720914011838 23.317939152984156 56-57 23.153787194080802 26.527795836736278 26.99728283762419 23.321134131558725 58-59 23.10427297388288 26.00750708697113 26.96606424100502 23.92215569814097 60-61 22.62904772789337 26.36650505267582 27.315381898491236 23.689065320939573 62-63 22.84652880678518 26.383926428385905 27.442942610237093 23.326602154591818 64-65 22.56602240427065 26.470461071967765 27.124096464827733 23.839420058933843 66-67 22.49071586288391 26.48150794446828 27.15277295394912 23.87500323869869 68-69 23.268526997571016 25.948263762332296 27.233825292639462 23.549383947457226 70-71 22.884487059700955 26.220521554024863 26.795204416636654 24.09978696963753 72-73 22.87329169898074 26.33709519721372 26.798443965215093 23.991169138590447 74-75 22.883898929316583 26.654196183129176 26.62528242180012 23.83662246575412 76-77 23.014320815905336 26.207534999321265 26.985265903284024 23.792878281489376 78-79 23.042630551434225 26.40905708075603 26.946989742052374 23.601322625757376 80-81 23.318097037760218 26.40187324415152 26.6192272979143 23.660802420173965 82-83 23.151247199991353 26.49350219963211 26.720586636384503 23.634663963992033 84-85 22.9620853758605 26.617047258401055 26.70461933567304 23.716248030065408 86-87 23.271399089528625 26.597008723694877 26.319343407682084 23.812248779094414 88-89 22.994891687790357 26.71481814284549 26.139801202298035 24.150488967066114 90-91 22.83888340050532 26.649573641346898 26.431232231744545 24.08031072640324 92-93 23.01837516491552 26.17864920284211 26.658151992497338 24.144823639745034 94-95 22.726915802936265 25.945912350769863 26.841507762844213 24.485664083449663 96-97 23.082531714141247 25.514324076742444 26.819639933805117 24.58350427531119 98-99 22.66924723443608 25.97000980111338 26.41524039749984 24.945502566950697 100-101 22.722354499688368 25.48425394793794 26.901087875526404 24.892303676847288 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 268.0 1 202.0 2 188.5 3 258.5 4 244.5 5 249.5 6 463.5 7 1006.5 8 1618.0 9 1853.5 10 1641.0 11 1444.5 12 1465.5 13 1586.5 14 1821.5 15 2102.0 16 2335.5 17 2584.0 18 2895.0 19 3248.0 20 3779.5 21 4526.5 22 5542.5 23 6826.5 24 8476.0 25 10928.5 26 14195.0 27 18177.0 28 21771.5 29 25345.5 30 29939.5 31 37437.5 32 49457.5 33 53232.0 34 50695.0 35 53166.5 36 59006.0 37 64988.0 38 69262.0 39 73981.0 40 80121.0 41 86590.0 42 90872.5 43 95076.0 44 99367.0 45 106050.0 46 110360.5 47 113626.5 48 115949.0 49 114738.0 50 115187.0 51 114729.5 52 115283.5 53 120673.5 54 127339.5 55 122197.0 56 111915.5 57 103905.0 58 97610.5 59 87519.0 60 70306.0 61 58391.0 62 50405.5 63 38397.5 64 26899.5 65 20224.0 66 15273.0 67 11802.5 68 9146.0 69 7124.5 70 5653.5 71 4037.0 72 2896.0 73 2290.0 74 1694.5 75 1233.0 76 913.0 77 624.0 78 425.5 79 270.5 80 143.0 81 87.0 82 57.5 83 34.0 84 19.5 85 9.0 86 7.0 87 5.0 88 3.0 89 3.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.2716332635100703E-4 2 0.04422104673856269 3 1.2716332635100703E-4 4 0.0 5 0.10360632014448296 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012557378477161944 26-27 0.012779914298276206 28-29 0.0 30-31 0.0 32-33 5.086533054040281E-4 34-35 0.016404069099279908 36-37 0.003735422711560831 38-39 0.013320358435267986 40-41 0.007629799581060422 42-43 0.0060243625858789574 44-45 0.0030519198324241684 46-47 4.768624738162763E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.3843123690813818E-4 68-69 3.020129000836417E-4 70-71 0.0 72-73 4.768624738162763E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.358166317550351E-5 82-83 0.0011126791055713116 84-85 0.004832206401338267 86-87 0.0015100645004182084 88-89 4.768624738162763E-5 90-91 9.06038700250925E-4 92-93 3.496991474652693E-4 94-95 0.0 96-97 1.5895415793875878E-5 98-99 0.0 100-101 8.424570370754215E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3145561.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.055579469654 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46533788908665 31.043912932286784 2 14.965998106385376 13.186714378371045 3 5.678716683130238 7.505374623578826 4 2.775225663586593 4.890566990734497 5 1.582546524678964 3.486000209120944 6 0.9530673663051161 2.5192761057713327 7 0.6736490474807367 2.077459940216102 8 0.4729938430639518 1.6670414273408787 9 0.3588478767954295 1.4228326038309889 >10 1.8445849313782736 14.893716387403078 >50 0.14321081730912483 4.34366700861548 >100 0.07532159221286565 6.117135841148275 >500 0.005503269328348006 1.5675650399619738 >1k 0.004561920627024268 4.163754914737971 >5k 4.344686311451685E-4 1.1149815968817562 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7548 0.23995719682435024 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5972 0.18985484624205348 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5579 0.17736104942806705 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5505 0.17500852789057342 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5266 0.16741051914110075 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5081 0.16152921529736666 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4678 0.14871751016750273 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4623 0.14696901443017638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4246 0.13498387092159395 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4119 0.13094643530994948 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3818 0.1213773950020362 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3630 0.11540071866353886 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3374 0.10726226577707441 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3306 0.1051004892291073 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3235 0.10284334018637693 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3193 0.10150812525969136 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.1790831587751756E-5 0.0 5 0.0 0.0 0.0 3.1790831587751756E-5 0.0 6 0.0 0.0 0.0 3.1790831587751756E-5 3.1790831587751756E-5 7 0.0 0.0 0.0 3.1790831587751756E-5 3.1790831587751756E-5 8 0.0 0.0 0.0 6.358166317550351E-5 3.1790831587751756E-5 9 0.0 0.0 0.0 6.358166317550351E-5 3.1790831587751756E-5 10-11 0.0 0.0 0.0 6.358166317550351E-5 3.1790831587751756E-5 12-13 0.0 0.0 0.0 6.358166317550351E-5 1.9074498952651052E-4 14-15 0.0 0.0 0.0 6.358166317550351E-5 1.9074498952651052E-4 16-17 0.0 0.0 0.0 1.2716332635100703E-4 2.225358211142623E-4 18-19 0.0 0.0 0.0 1.5895415793875877E-4 2.225358211142623E-4 20-21 0.0 0.0 0.0 2.0664040532038643E-4 2.3843123690813818E-4 22-23 0.0 0.0 0.0 3.973853948468969E-4 2.5432665270201405E-4 24-25 0.0 0.0 0.0 7.152937107244145E-4 2.5432665270201405E-4 26-27 0.0 0.0 0.0 0.001303424095097822 2.861174842897658E-4 28-29 0.0 0.0 0.0 0.003767213543148583 2.861174842897658E-4 30-31 0.0 0.0 0.0 0.013320358435267986 2.861174842897658E-4 32-33 0.0 0.0 0.0 0.034620215599061664 3.1790831587751755E-4 34-35 0.0 0.0 0.0 0.06684022341324806 4.132808106407728E-4 36-37 0.0 0.0 0.0 0.1183413705854059 4.132808106407728E-4 38-39 0.0 0.0 0.0 0.1976753908126404 4.7686247381627635E-4 40-41 0.0 0.0 0.0 0.28180982660962545 4.7686247381627635E-4 42-43 0.0 0.0 0.0 0.3718732524977262 4.7686247381627635E-4 44-45 0.0 0.0 0.0 0.48547778917655704 5.563395527856557E-4 46-47 0.0 0.0 0.0 0.6163447474075372 6.358166317550351E-4 48-49 0.0 0.0 0.0 0.7556203805934776 6.358166317550351E-4 50-51 0.0 0.0 0.0 0.9102350900205083 6.676074633427868E-4 52-53 0.0 0.0 0.0 1.059095658930156 6.993982949305386E-4 54-55 0.0 0.0 0.0 1.2248689502444874 6.993982949305386E-4 56-57 0.0 0.0 0.0 1.4163292334817223 6.993982949305386E-4 58-59 0.0 0.0 0.0 1.6137820884732483 6.993982949305386E-4 60-61 0.0 0.0 0.0 1.8329162906076215 6.993982949305386E-4 62-63 0.0 0.0 0.0 2.0501112520151414 6.993982949305386E-4 64-65 0.0 0.0 0.0 2.2782740503204355 6.993982949305386E-4 66-67 0.0 0.0 0.0 2.549354471269195 7.152937107244145E-4 68-69 0.0 0.0 0.0 2.845454276677515 7.629799581060421E-4 70-71 0.0 0.0 0.0 3.1479758300665606 7.947707896937938E-4 72-73 0.0 0.0 0.0 3.4671080929602063 8.424570370754215E-4 74-75 0.0 0.0 0.0 3.798098336036084 8.583524528692975E-4 76-77 0.0 0.0 0.0 4.156492275940604 8.583524528692975E-4 78-79 0.0 0.0 0.0 4.557708465993825 8.583524528692975E-4 80-81 0.0 0.0 0.0 4.975694319709584 8.583524528692975E-4 82-83 0.0 0.0 0.0 5.408955668003259 9.06038700250925E-4 84-85 0.0 1.5895415793875878E-5 0.0 5.858144222922398 9.21934116044801E-4 86-87 3.1790831587751756E-5 3.1790831587751756E-5 0.0 6.320319332545132 0.001049097442395808 88-89 3.1790831587751756E-5 3.1790831587751756E-5 0.0 6.806591892511383 0.001176260768746815 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4245 0.0 53.443577 1 GTATCAA 7900 0.0 44.06957 1 ATCAACG 10520 0.0 32.98842 3 TATCAAC 10720 0.0 32.387905 2 TCAACGC 10725 0.0 32.357872 4 CAACGCA 10905 0.0 31.910954 5 AACGCAG 11485 0.0 30.312366 6 ACGCAGA 13105 0.0 26.420279 7 TAAGGTG 2125 0.0 26.39839 5 CGCAGAG 13435 0.0 25.700623 8 TGGTATC 2190 0.0 25.19235 2 AGGTAAG 2005 0.0 24.670263 2 GTAAGGT 2210 0.0 24.522623 4 TACATGG 9970 0.0 24.09079 2 GTACATG 10330 0.0 23.711645 1 GTGGTAT 2570 0.0 23.503113 1 GTGTAAG 1360 0.0 23.081276 1 GGTAAGG 2280 0.0 22.727205 3 AAGGTAA 2060 0.0 22.626305 1 ACATGGG 10215 0.0 22.478283 3 >>END_MODULE