FastQCFastQC Report
Thu 26 May 2016
SRR938424_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938424_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3167713
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73540.23215487009081948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55960.17665741814362604No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA55240.17438448495807543No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54180.1710382222126815No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA51400.16226217463513898No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49020.15474886771623567No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC42550.13432403756274636No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA41300.13037797300449883No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG40920.12917836937879157No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA40800.12879954718119982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38570.1217597680092862No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36120.11402548147512101No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33650.10622805790802387No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT33410.10547041351284034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41900.056.5679971
GTATCAA80200.044.8358421
ATCAACG106300.033.578193
TATCAAC107750.033.2145422
TCAACGC109150.032.7449764
CAACGCA111400.032.0399365
AACGCAG117250.030.3936186
TGGTATC19250.027.6527212
ACGCAGA135300.026.2277327
CGCAGAG137650.025.676438
GTGGTAT24550.024.670651
TAAGGTG21750.024.036455
GTACATG102900.023.775541
TACCGTA1801.9513573E-723.7524247
TACATGG100900.023.457882
AGGTAAG19350.023.3342172
GTGTAAG13700.022.97481
GCAGAGT152650.022.4676539
TAAGGTA10800.022.4438324
GTAAGGT21550.022.054814
Produced by FastQC (version 0.11.5)