##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938424_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3167713 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.116025662678407 31.0 31.0 34.0 28.0 34.0 2 31.28978887923243 31.0 31.0 34.0 28.0 34.0 3 31.34722842631261 31.0 31.0 34.0 28.0 34.0 4 35.020601298160535 37.0 35.0 37.0 32.0 37.0 5 35.01108054927956 37.0 35.0 37.0 32.0 37.0 6 34.9885125325432 37.0 35.0 37.0 32.0 37.0 7 34.90566222381889 37.0 35.0 37.0 32.0 37.0 8 34.8534510544358 37.0 35.0 37.0 32.0 37.0 9 36.34724673605216 39.0 35.0 39.0 32.0 39.0 10-11 36.188641458364444 39.0 35.0 39.0 32.0 39.0 12-13 36.016576312311116 38.0 35.0 39.0 30.0 39.0 14-15 37.1737831047194 39.0 36.0 41.0 31.0 41.0 16-17 37.09848682630023 39.0 36.0 41.0 31.0 41.0 18-19 37.088194069349086 39.0 36.0 41.0 31.0 41.0 20-21 37.00845515360767 39.0 36.0 41.0 31.0 41.0 22-23 36.86770850136992 39.0 36.0 41.0 30.0 41.0 24-25 36.727051503718926 39.0 36.0 41.0 30.0 41.0 26-27 36.5088585992481 39.0 36.0 41.0 30.0 41.0 28-29 36.28488044844971 39.0 35.0 40.0 29.5 41.0 30-31 36.00821886326192 38.5 35.0 40.0 28.5 41.0 32-33 35.71394662332099 38.0 35.0 40.0 27.0 41.0 34-35 35.41576967357838 38.0 34.0 40.0 26.5 41.0 36-37 35.19665875664872 38.0 34.0 40.0 25.5 41.0 38-39 34.95164602980131 38.0 34.0 40.0 25.0 41.0 40-41 34.67431440285152 38.0 33.5 40.0 24.5 41.0 42-43 34.258685524856574 38.0 33.0 40.0 23.5 41.0 44-45 33.803745478204625 38.0 33.0 40.0 22.5 41.0 46-47 33.33021173319679 37.5 32.0 40.0 20.0 41.0 48-49 32.83932698448376 37.0 31.0 40.0 17.0 41.0 50-51 32.09436997606791 36.0 30.5 38.5 15.0 40.5 52-53 32.07736638388642 36.0 31.0 39.0 13.5 40.0 54-55 32.05513267773943 36.0 31.0 39.0 10.5 40.0 56-57 31.619123796884377 36.0 30.0 39.0 7.5 40.0 58-59 31.154975529664462 35.5 29.5 39.0 4.5 40.0 60-61 30.598538914352403 35.0 28.5 38.5 2.0 40.0 62-63 29.878921954103795 34.5 28.0 38.0 2.0 40.0 64-65 29.20792824349933 34.0 26.5 38.0 2.0 40.0 66-67 28.503564085509012 34.0 26.0 37.0 2.0 39.0 68-69 27.659231754896986 33.0 24.5 36.0 2.0 39.0 70-71 26.82576893803195 32.5 22.5 36.0 2.0 39.0 72-73 25.941893252324313 31.5 20.0 35.0 2.0 38.0 74-75 25.02078660535219 31.0 16.5 35.0 2.0 37.0 76-77 24.075590339150043 30.5 8.5 34.5 2.0 36.5 78-79 23.0881653419991 29.5 4.0 34.0 2.0 36.0 80-81 22.13782798504789 29.0 2.0 34.0 2.0 35.0 82-83 21.13419413311749 28.0 2.0 34.0 2.0 35.0 84-85 20.157611658631954 26.5 2.0 33.0 2.0 35.0 86-87 19.166213447998604 25.5 2.0 32.5 2.0 35.0 88-89 18.046740345479527 23.5 2.0 32.0 2.0 34.0 90-91 16.87820077134513 19.0 2.0 31.5 2.0 34.0 92-93 15.714245261486756 9.5 2.0 31.0 2.0 34.0 94-95 14.446082078774182 2.0 2.0 30.5 2.0 34.0 96-97 13.011322995486017 2.0 2.0 29.5 2.0 34.0 98-99 11.283640279280352 2.0 2.0 28.0 2.0 34.0 100-101 7.933766095602726 2.0 2.0 10.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47039482205317995 1101 2 0.3507760114565137 1101 3 0.3920097838632408 1101 4 0.28804455836109355 1101 5 0.32159242385958464 1101 6 0.36079271891688336 1101 7 0.34522893238192154 1101 8 0.41239752297303056 1101 9 0.4353806071177999 1101 10-11 0.5322065138485712 1101 12-13 0.41753080733331416 1101 14-15 0.5477062762330434 1101 16-17 0.5634933850663586 1101 18-19 0.4122143073211504 1101 20-21 0.6178948566191167 1101 22-23 0.6800703683961302 1101 24-25 0.6780405488954884 1101 26-27 0.7041179700719837 1101 28-29 0.6959376816072051 1101 30-31 0.8722078213207354 1101 32-33 0.8767231504456419 1101 34-35 0.9390903432732429 1101 36-37 1.0166939602107163 1101 38-39 1.0696629544556302 1101 40-41 1.0313121322572982 1101 42-43 1.2146401364646096 1101 44-45 1.2631959262102725 1101 46-47 1.3473191844188435 1101 48-49 1.2072296999807506 1101 50-51 1.3305811751380219 1101 52-53 1.3987680008813328 1101 54-55 1.6140031713517615 1101 56-57 1.8171965464703135 1101 58-59 1.5423039999237353 1101 60-61 1.697409506991658 1101 62-63 1.6629657716480786 1101 64-65 1.6617938507869923 1101 66-67 1.8586403909078122 1101 68-69 1.8784041328025474 1101 70-71 2.086976359944014 1101 72-73 2.298663592759933 1101 74-75 2.137315909261307 1101 76-77 2.55328495288067 1101 78-79 2.579851436316627 1101 80-81 3.0583011427441598 1101 82-83 3.222811060953628 1101 84-85 3.2366455065255764 1101 86-87 3.4278679182786966 1101 88-89 3.724996117216719 1101 90-91 3.7820641348227504 1101 92-93 3.9801327351694216 1101 94-95 4.076045580874618 1101 96-97 3.7453476657001037 1101 98-99 3.2803474350366546 1101 100-101 2.537436661999383 1102 1 0.7028042543465816 1102 2 0.5214695801305673 1102 3 0.48296633770199193 1102 4 0.3447949982769387 1102 5 0.39826511506613826 1102 6 0.5048887216709588 1102 7 0.501221794183536 1102 8 0.5214272817114676 1102 9 0.4979246635099841 1102 10-11 0.6662973216035297 1102 12-13 0.7261114215875253 1102 14-15 0.7022181485169483 1102 16-17 0.8434173576541824 1102 18-19 0.7079732282545024 1102 20-21 0.6299269826595406 1102 22-23 0.6356052199964779 1102 24-25 0.7652075530815594 1102 26-27 0.6421386754097611 1102 28-29 0.8184741788090619 1102 30-31 0.7181552553955726 1102 32-33 0.7892196637820703 1102 34-35 0.8653819123392523 1102 36-37 0.8498719620336317 1102 38-39 1.02792124027183 1102 40-41 1.088534477182428 1102 42-43 1.2735934219127003 1102 44-45 1.3581541847553282 1102 46-47 1.4343069559508663 1102 48-49 1.4218895001236405 1102 50-51 1.429519207405093 1102 52-53 1.3393527649132793 1102 54-55 1.3351886577063325 1102 56-57 1.5829246914408586 1102 58-59 1.605535901158614 1102 60-61 1.6449595101542762 1102 62-63 1.653419364544721 1102 64-65 1.7698495731148078 1102 66-67 1.816901683809327 1102 68-69 1.853502148229932 1102 70-71 1.839207565401562 1102 72-73 1.944120353465415 1102 74-75 2.1082439268973445 1102 76-77 2.3967791756476196 1102 78-79 2.7796197870547665 1102 80-81 2.993015868855718 1102 82-83 3.2042061722624453 1102 84-85 3.3736019110469897 1102 86-87 3.3408460837285325 1102 88-89 3.6893043994555264 1102 90-91 3.530441604910031 1102 92-93 3.6085385962897814 1102 94-95 3.4512351050189185 1102 96-97 3.543404881201317 1102 98-99 3.171172817740107 1102 100-101 2.294559306492424 1103 1 0.5894749534399253 1103 2 0.5458697899552512 1103 3 0.5609553406831651 1103 4 0.46116959366926125 1103 5 0.40552438199014773 1103 6 0.42627807510569937 1103 7 0.47988965113520266 1103 8 0.4117163779391717 1103 9 0.5409292355467272 1103 10-11 0.6269212843656433 1103 12-13 0.6176600352344437 1103 14-15 0.6227789101821557 1103 16-17 0.6099733623077199 1103 18-19 0.8258174354232182 1103 20-21 0.8201932167059525 1103 22-23 0.8389671192374735 1103 24-25 0.7896988021297204 1103 26-27 0.9220397917314216 1103 28-29 0.8622909126073708 1103 30-31 0.9214881399416655 1103 32-33 0.9634156021642397 1103 34-35 0.9920406939338093 1103 36-37 1.067872748676443 1103 38-39 1.2022844750877013 1103 40-41 1.2822496146032165 1103 42-43 1.3130754801442421 1103 44-45 1.4104139450789646 1103 46-47 1.5477322900043973 1103 48-49 1.3827031320962533 1103 50-51 1.372665487012064 1103 52-53 1.4444217669515993 1103 54-55 1.377620826243664 1103 56-57 1.6016098053942045 1103 58-59 1.601525081523949 1103 60-61 1.807852352144149 1103 62-63 1.9035464598951002 1103 64-65 2.0004637617700496 1103 66-67 2.1589285055726073 1103 68-69 2.227241182572943 1103 70-71 2.3139211339313768 1103 72-73 2.652949500159721 1103 74-75 2.6227849833398658 1103 76-77 2.7868428461733252 1103 78-79 3.1383572752790947 1103 80-81 2.992029019653824 1103 82-83 3.0671340116674592 1103 84-85 3.2298834876246083 1103 86-87 3.2818043756788846 1103 88-89 3.5031618477124873 1103 90-91 3.397113788197405 1103 92-93 3.3018089249538587 1103 94-95 3.3153771669579495 1103 96-97 3.189815279029242 1103 98-99 2.894375774382466 1103 100-101 2.037945604045561 1104 1 0.5248083004128148 1104 2 0.44849765890336357 1104 3 0.44155797796719654 1104 4 0.3436286314288566 1104 5 0.35337401118364653 1104 6 0.4874671148127234 1104 7 0.351968702216098 1104 8 0.45113128642911704 1104 9 0.5922326277802128 1104 10-11 0.46629831467046046 1104 12-13 0.6470188491537598 1104 14-15 0.5891442373685933 1104 16-17 0.6181163196363713 1104 18-19 0.6228403748840492 1104 20-21 0.6043437773296105 1104 22-23 0.6779392252077372 1104 24-25 0.6210737698304953 1104 26-27 0.659912236871989 1104 28-29 0.8765300886720766 1104 30-31 0.7142020214487701 1104 32-33 0.8649189654515581 1104 34-35 0.7909997873882659 1104 36-37 0.8907057546604165 1104 38-39 0.8359076111096968 1104 40-41 0.9620889197890463 1104 42-43 1.123919931610537 1104 44-45 1.0202293772521003 1104 46-47 0.992110509981643 1104 48-49 1.003630445762866 1104 50-51 1.0793819645814295 1104 52-53 1.2237900527851693 1104 54-55 1.472299997352657 1104 56-57 1.608644832912006 1104 58-59 1.69600960479492 1104 60-61 1.8157186348572942 1104 62-63 1.7900801730900788 1104 64-65 1.8573234357391861 1104 66-67 1.9228078909741875 1104 68-69 2.2426768034872318 1104 70-71 2.1139652351641622 1104 72-73 2.3642685229461122 1104 74-75 2.121226427208164 1104 76-77 2.4476463031947056 1104 78-79 2.467397937070377 1104 80-81 2.622921923750315 1104 82-83 2.8940283850472888 1104 84-85 3.010433536840676 1104 86-87 3.179662898999876 1104 88-89 3.3593197949137625 1104 90-91 3.1113042217913325 1104 92-93 2.784821899203136 1104 94-95 2.9280737660103053 1104 96-97 2.813838961467022 1104 98-99 2.549870081383119 1104 100-101 1.9564869993418856 1105 1 0.560157048684033 1105 2 0.38692774633013727 1105 3 0.272335983410283 1105 4 0.2848258688944014 1105 5 0.43137841027085955 1105 6 0.5217523565509694 1105 7 0.5438683045619115 1105 8 0.41597787609252634 1105 9 0.48679712309127865 1105 10-11 0.5317535564581632 1105 12-13 0.49924309160797264 1105 14-15 0.622462679962041 1105 16-17 0.7065307745336398 1105 18-19 0.6506984216255915 1105 20-21 0.6581194041006384 1105 22-23 0.5157966510438854 1105 24-25 0.6826342039807258 1105 26-27 0.6733695369885382 1105 28-29 0.7547712285444774 1105 30-31 0.717966466428031 1105 32-33 0.744079787090719 1105 34-35 0.866591996509193 1105 36-37 0.9694002928359353 1105 38-39 0.9527162618053566 1105 40-41 0.9644163534222301 1105 42-43 1.061175656165915 1105 44-45 1.1072202829112925 1105 46-47 1.341937535630528 1105 48-49 1.1835644319809617 1105 50-51 1.1077556924809073 1105 52-53 1.0591087723134152 1105 54-55 1.239554298770905 1105 56-57 1.475633024170648 1105 58-59 1.5244985024021283 1105 60-61 1.5103138182798936 1105 62-63 1.5434698769401898 1105 64-65 1.7208816741921318 1105 66-67 1.8906382607205998 1105 68-69 2.0144999795227037 1105 70-71 1.9602404621930027 1105 72-73 2.205431033667722 1105 74-75 2.401288825452486 1105 76-77 2.639562801859757 1105 78-79 2.6854607342326737 1105 80-81 2.819761432894385 1105 82-83 3.127465871138728 1105 84-85 3.2135411683173913 1105 86-87 3.333313355640076 1105 88-89 3.268582439542133 1105 90-91 3.112917614226067 1105 92-93 3.1581312370157786 1105 94-95 2.9785263126290342 1105 96-97 3.04163417587994 1105 98-99 2.7874071914696437 1105 100-101 1.916696304900416 1106 1 0.19428813108546095 1106 2 0.13929259575663266 1106 3 0.1740334306567881 1106 4 0.2030288444592543 1106 5 0.2001116952953339 1106 6 -0.11878563445313972 1106 7 0.1251653757826645 1106 8 -0.06979494603728398 1106 9 0.1877271775149154 1106 10-11 0.3207569794240257 1106 12-13 0.3305654596997485 1106 14-15 0.40695265152074 1106 16-17 -0.11955078910676065 1106 18-19 0.3694061921715388 1106 20-21 0.3530636204198956 1106 22-23 0.21905836975138016 1106 24-25 0.3999824717162781 1106 26-27 0.3936889630270102 1106 28-29 0.41353520710578806 1106 30-31 0.18657114542434527 1106 32-33 0.21333157636832567 1106 34-35 0.1082870065517838 1106 36-37 0.2545977607551464 1106 38-39 0.3825141806072949 1106 40-41 0.38055885804094913 1106 42-43 0.05714828549549367 1106 44-45 0.5682971027083497 1106 46-47 0.6922872308712371 1106 48-49 0.5429315097081826 1106 50-51 0.4149075803347131 1106 52-53 0.5261186603978771 1106 54-55 0.42046310382287544 1106 56-57 0.8722942180088751 1106 58-59 0.8452002648052108 1106 60-61 1.0929059260913476 1106 62-63 1.0807011588876883 1106 64-65 1.0369647175634817 1106 66-67 1.4140792118394323 1106 68-69 1.5706786178655783 1106 70-71 1.4037049475621615 1106 72-73 1.4982687006542719 1106 74-75 1.9286945850876016 1106 76-77 1.9599329848458993 1106 78-79 2.2835617280460916 1106 80-81 2.190703325165618 1106 82-83 2.3178150332410787 1106 84-85 2.3021678039097075 1106 86-87 2.1091131719793488 1106 88-89 1.940455909489451 1106 90-91 2.0505469546795183 1106 92-93 2.1303073989853853 1106 94-95 2.048825773180061 1106 96-97 2.033155371937763 1106 98-99 1.7095548468577135 1106 100-101 1.2449185822498716 1107 1 0.2738989342329923 1107 2 0.2744699281429597 1107 3 0.20717266988635075 1107 4 0.1258517474234111 1107 5 0.0798938279909791 1107 6 0.07969292273632789 1107 7 0.14298126726367855 1107 8 0.1329139660410945 1107 9 0.20581134052874717 1107 10-11 0.2293375008094003 1107 12-13 0.27714545294742976 1107 14-15 0.28908426147214783 1107 16-17 0.23133559264572767 1107 18-19 0.37107028119005747 1107 20-21 0.3723879263811227 1107 22-23 0.21388311069074462 1107 24-25 0.27144608555096994 1107 26-27 0.24109989757856454 1107 28-29 0.3088221035473637 1107 30-31 0.34290585583817546 1107 32-33 0.4087367434321436 1107 34-35 0.409496399837856 1107 36-37 0.4929050094099381 1107 38-39 0.7077365027600777 1107 40-41 0.5512618555375539 1107 42-43 0.5774414859678743 1107 44-45 0.507883999570204 1107 46-47 0.6996384312287489 1107 48-49 0.6281835448594535 1107 50-51 0.7091235270110161 1107 52-53 0.7346143225242443 1107 54-55 0.755378048438466 1107 56-57 0.9454241272728581 1107 58-59 0.8996679664536451 1107 60-61 1.0606275372895482 1107 62-63 1.255601239999553 1107 64-65 1.126725885854487 1107 66-67 1.3491797401172114 1107 68-69 1.419770627296412 1107 70-71 1.4998195282250357 1107 72-73 1.4392932667618403 1107 74-75 1.651066469670603 1107 76-77 1.8983151113776024 1107 78-79 2.23005469064638 1107 80-81 2.2953279971809053 1107 82-83 2.467058059231899 1107 84-85 2.506333297326197 1107 86-87 2.4484430394966417 1107 88-89 2.482846839249941 1107 90-91 2.4416541035905333 1107 92-93 2.2880594656568203 1107 94-95 2.4628927275907113 1107 96-97 2.2664276262171494 1107 98-99 2.086292482902202 1107 100-101 1.337790614627373 1108 1 0.2139145800627702 1108 2 0.20126332715723905 1108 3 0.05679529449963994 1108 4 0.08973877304303102 1108 5 0.14783795582044945 1108 6 0.1447890364541422 1108 7 0.15153139221066425 1108 8 0.10968734312417894 1108 9 0.29982182627600196 1108 10-11 0.2675647319875125 1108 12-13 0.20667294381929224 1108 14-15 0.27245651225823764 1108 16-17 0.4150610150915881 1108 18-19 0.23238917733115017 1108 20-21 0.2578925550798985 1108 22-23 0.23338388031012158 1108 24-25 0.36662726290810355 1108 26-27 0.3108105173122482 1108 28-29 0.3473157869237582 1108 30-31 0.3711608558297357 1108 32-33 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0.19162230893361532 2316 94-95 0.10624563971545165 2316 96-97 0.17075279686112843 2316 98-99 -0.06403476621101056 2316 100-101 0.1978800096826756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9875.0 3 3473.0 4 3877.0 5 4377.0 6 5559.0 7 7196.0 8 9290.0 9 12007.0 10 15471.0 11 19518.0 12 24470.0 13 28687.0 14 33089.0 15 37785.0 16 44189.0 17 50320.0 18 50871.0 19 52125.0 20 56400.0 21 62477.0 22 70180.0 23 77786.0 24 86915.0 25 96209.0 26 107051.0 27 118175.0 28 131969.0 29 146215.0 30 163457.0 31 182775.0 32 204496.0 33 231252.0 34 258336.0 35 278534.0 36 265629.0 37 176100.0 38 41042.0 39 536.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68614180769171 17.95798292963426 14.408338131792247 23.947537130881784 2 15.91636540934365 19.403497869093904 39.43865718811341 25.241479533449034 3 19.490101894353888 22.918729265454292 29.134616447419326 28.456552392772494 4 13.329200169663945 15.870053586898608 35.68008807870739 35.12065816473005 5 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23.50109769821989 26.495279353028177 26.86028946650146 23.143333482250473 52-53 23.852118739184668 25.97995061531948 26.993801499599513 23.174129145896337 54-55 23.10361555028066 26.375368182671327 27.163834362747796 23.35718190430021 56-57 23.188029911015203 26.538322501614104 26.952258875195547 23.321388712175146 58-59 23.186261316900037 25.904985000248615 26.945599165286133 23.96315451756522 60-61 22.628735292339446 26.274882282676593 27.41222050176491 23.684161923219055 62-63 22.936697734472318 26.210759602875555 27.550504456528618 23.302038206123505 64-65 22.62651229266109 26.36611870903459 27.18461391291884 23.822755085385477 66-67 22.537253968484478 26.36070668415214 27.20876681329797 23.893272534065417 68-69 23.36387861215378 25.770047420925792 27.373039196573558 23.49303477034687 70-71 23.01531798167374 26.016241809161112 26.836999069857903 24.13144113930725 72-73 23.033338663798215 26.196982725347244 26.84623650499151 23.92344210586303 74-75 23.070602485679064 26.549539695201297 26.746000185962604 23.63385763315704 76-77 23.277229809242755 26.084642262094093 26.86956542328629 23.76856250537686 78-79 23.337793887057643 26.364571890239752 26.75855502053817 23.53907920216443 80-81 23.616564585208934 26.31737806384476 26.410911294662515 23.655146056283797 82-83 23.442548495153286 26.40110922742272 26.506058332316417 23.650283945107578 84-85 23.33348171875784 26.500516033992263 26.495354115501318 23.670648131748575 86-87 23.721803532149952 26.462380637813755 26.05423476792889 23.761581062107403 88-89 23.507784494096686 26.541778813615913 25.90639915267977 24.044037539607626 90-91 23.38011735608565 26.54920532420546 26.018695189158336 24.051982130550552 92-93 23.673654569099345 25.930917861126606 26.26828392738239 24.127143642391655 94-95 23.38179909299379 25.790567609063746 26.461625901381826 24.366007396560637 96-97 23.806028020798994 25.345160646602732 26.34764685154382 24.50116448105445 98-99 23.520574241233096 25.637938818745244 25.814839722035416 25.02664721798624 100-101 24.572544516389648 24.538486837062532 26.086143645018783 24.802825001529037 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 209.0 1 179.0 2 188.0 3 258.0 4 239.0 5 217.5 6 392.0 7 879.0 8 1442.0 9 1677.5 10 1522.0 11 1324.0 12 1352.5 13 1495.0 14 1694.0 15 1890.0 16 2153.0 17 2477.5 18 2790.0 19 3274.5 20 3802.5 21 4488.0 22 5555.5 23 6836.0 24 8596.5 25 11173.5 26 14363.5 27 18254.5 28 21881.5 29 25318.0 30 29933.5 31 37348.0 32 47625.5 33 51673.5 34 51121.0 35 53749.5 36 58958.5 37 64854.0 38 69935.0 39 74695.0 40 80064.5 41 86476.0 42 91297.5 43 95278.0 44 98972.5 45 104914.0 46 109591.0 47 112750.0 48 115744.0 49 115417.0 50 114392.0 51 114170.5 52 115710.0 53 119468.5 54 123201.5 55 119782.0 56 112162.0 57 104726.5 58 98696.0 59 88669.5 60 73197.5 61 61537.5 62 52404.0 63 41519.0 64 31157.0 65 23841.0 66 18300.5 67 13974.0 68 10878.5 69 8599.5 70 6671.0 71 4945.5 72 3628.0 73 2817.0 74 2115.0 75 1525.5 76 1104.5 77 802.0 78 552.0 79 348.5 80 205.0 81 128.5 82 89.5 83 56.0 84 39.0 85 25.0 86 19.0 87 12.5 88 8.0 89 6.0 90 4.0 91 4.0 92 3.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3846939416544365 2 0.031094988718990642 3 0.03781908272624446 4 0.04590062294153543 5 0.06427349952473599 6 0.05492921865080581 7 0.039302803000145534 8 0.041228482504570334 9 0.03753496607805063 10-11 0.03960270390657234 12-13 0.03144224240011642 14-15 0.02912195643986687 16-17 0.02375530864065021 18-19 0.028648428692877165 20-21 0.035656639348324796 22-23 0.03486742643667529 24-25 0.04279112406963636 26-27 0.03736133923748774 28-29 0.032673414542289655 30-31 0.035530365282460875 32-33 0.03016371748324422 34-35 0.03177371182300922 36-37 0.03409399778325878 38-39 0.016494549853474733 40-41 0.014190048151458166 42-43 0.014868771255476743 44-45 0.01281681768518802 46-47 0.02111933751574085 48-49 0.018404445099666544 50-51 0.012737896394023071 52-53 0.010543884499637435 54-55 0.009391633648629154 56-57 0.01003878823618175 58-59 0.005066746892789845 60-61 0.008633989253445624 62-63 0.012422211229363265 64-65 0.008018403182359008 66-67 0.004135475657043425 68-69 0.00724497452894249 70-71 0.014363674992021059 72-73 0.012138094581169443 74-75 0.012927307492818953 76-77 0.006487330133758961 78-79 0.01005457249441474 80-81 0.012295937163499344 82-83 0.014632007381981891 84-85 0.009028595709270379 86-87 0.003014793322501123 88-89 0.01741003683098816 90-91 0.027006865836646188 92-93 0.012059173290004492 94-95 0.02211374578441923 96-97 0.02010914498882948 98-99 0.012722112135790078 100-101 0.12509024649644712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3167713.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.01323407516183 #Duplication Level Percentage of deduplicated Percentage of total 1 73.15038719723906 34.39036275992521 2 13.812152748226186 12.98707940468495 3 5.15494663468796 7.270521383446585 4 2.4836026947565935 4.670487793531778 5 1.3828165515128554 3.250533910964098 6 0.8832302831416492 2.491410722616589 7 0.5792842901926716 1.9063819551624432 8 0.4198815222364897 1.5791990630991486 9 0.3014962360851427 1.275688180786596 >10 1.633637540078263 14.12542486381658 >50 0.1238020322434122 4.014749578994094 >100 0.06596940489150631 5.7331460747340035 >500 0.004328794933696168 1.333026552587833 >1k 0.004125882670391482 4.048812572117177 >5k 3.381871041304494E-4 0.9231751835329969 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7354 0.23215487009081948 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5596 0.17665741814362604 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5524 0.17438448495807543 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5418 0.1710382222126815 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5140 0.16226217463513898 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4902 0.15474886771623567 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4255 0.13432403756274636 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4130 0.13037797300449883 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4092 0.12917836937879157 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4080 0.12879954718119982 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3857 0.1217597680092862 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3612 0.11402548147512101 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3365 0.10622805790802387 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3341 0.10547041351284034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5784258232990172E-4 2 0.0 0.0 0.0 0.0 1.5784258232990172E-4 3 0.0 0.0 0.0 0.0 1.5784258232990172E-4 4 0.0 0.0 0.0 0.0 1.5784258232990172E-4 5 0.0 0.0 0.0 0.0 1.5784258232990172E-4 6 0.0 0.0 0.0 0.0 1.5784258232990172E-4 7 0.0 0.0 0.0 0.0 1.5784258232990172E-4 8 0.0 0.0 0.0 0.0 1.8941109879588207E-4 9 0.0 0.0 0.0 0.0 1.8941109879588207E-4 10-11 0.0 0.0 0.0 6.31370329319607E-5 1.8941109879588207E-4 12-13 0.0 0.0 0.0 7.892129116495087E-5 2.9990090642681324E-4 14-15 0.0 0.0 0.0 9.470554939794104E-5 3.314694228927936E-4 16-17 0.0 0.0 0.0 9.470554939794104E-5 3.7882219759176414E-4 18-19 0.0 0.0 0.0 9.470554939794104E-5 4.4195923052372485E-4 20-21 0.0 0.0 0.0 1.1048980763093121E-4 5.208805216886757E-4 22-23 0.0 0.0 0.0 2.525481317278428E-4 5.366647799216658E-4 24-25 0.0 0.0 0.0 4.893120052226953E-4 5.366647799216658E-4 26-27 0.0 0.0 0.0 0.0011522508510082826 6.945073622515676E-4 28-29 0.0 0.0 0.0 0.003835574750616612 6.945073622515676E-4 30-31 0.0 0.0 0.0 0.013479756530973608 7.260758787175479E-4 32-33 0.0 0.0 0.0 0.03324164783867731 7.260758787175479E-4 34-35 0.0 0.0 0.0 0.06406830416770712 7.576443951835283E-4 36-37 0.0 0.0 0.0 0.11471998883737258 7.576443951835283E-4 38-39 0.0 0.0 0.0 0.19133677830030688 8.20781428115489E-4 40-41 0.0 0.0 0.0 0.27526167932511564 8.20781428115489E-4 42-43 0.0 0.0 0.0 0.3652793040278586 8.523499445814693E-4 44-45 0.0 0.0 0.0 0.47916272717888264 9.312712357464202E-4 46-47 0.0 0.0 0.0 0.6097301112821774 0.0010101925269113711 48-49 0.0 0.0 0.0 0.7463902190634064 0.0010733295598433317 50-51 0.0 0.0 0.0 0.8995606609563429 0.0011048980763093122 52-53 0.0 0.0 0.0 1.0490060179062939 0.0011206823345423023 54-55 0.0 0.0 0.0 1.209326728778775 0.0011838193674742628 56-57 0.0 0.0 0.0 1.397617145240115 0.0012311721421732335 58-59 0.0 0.0 0.0 1.6007131959239995 0.0012943091751051942 60-61 0.0 0.0 0.0 1.8194356622585444 0.0012943091751051942 62-63 0.0 0.0 0.0 2.0328861863432706 0.0012943091751051942 64-65 0.0 0.0 0.0 2.2561071662742176 0.0013258776915711745 66-67 0.0 0.0 0.0 2.5193885936004934 0.0013258776915711745 68-69 0.0 0.0 0.0 2.808319440555379 0.0013258776915711745 70-71 0.0 3.156851646598035E-5 0.0 3.1101933792613154 0.0013258776915711745 72-73 0.0 3.156851646598035E-5 0.0 3.429256375182979 0.0013416619498041646 74-75 0.0 3.156851646598035E-5 0.0 3.7453992833315395 0.001373230466270145 76-77 1.5784258232990174E-5 3.156851646598035E-5 0.0 4.088186019377387 0.0013890147245031353 78-79 3.156851646598035E-5 3.156851646598035E-5 0.0 4.469754677901691 0.0014205832409691155 80-81 3.156851646598035E-5 3.156851646598035E-5 0.0 4.865892206775046 0.0014521517574350958 82-83 3.156851646598035E-5 3.156851646598035E-5 0.0 5.268864319463285 0.0014837202739010763 84-85 3.156851646598035E-5 3.156851646598035E-5 1.5784258232990174E-5 5.683706194342733 0.0014837202739010763 86-87 3.156851646598035E-5 3.156851646598035E-5 3.156851646598035E-5 6.114711149652763 0.0014837202739010763 88-89 3.156851646598035E-5 3.156851646598035E-5 3.156851646598035E-5 6.556623027401788 0.0014837202739010763 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4190 0.0 56.567997 1 GTATCAA 8020 0.0 44.835842 1 ATCAACG 10630 0.0 33.57819 3 TATCAAC 10775 0.0 33.214542 2 TCAACGC 10915 0.0 32.744976 4 CAACGCA 11140 0.0 32.039936 5 AACGCAG 11725 0.0 30.393618 6 TGGTATC 1925 0.0 27.652721 2 ACGCAGA 13530 0.0 26.227732 7 CGCAGAG 13765 0.0 25.67643 8 GTGGTAT 2455 0.0 24.67065 1 TAAGGTG 2175 0.0 24.03645 5 GTACATG 10290 0.0 23.77554 1 TACCGTA 180 1.9513573E-7 23.752424 7 TACATGG 10090 0.0 23.45788 2 AGGTAAG 1935 0.0 23.334217 2 GTGTAAG 1370 0.0 22.9748 1 GCAGAGT 15265 0.0 22.467653 9 TAAGGTA 1080 0.0 22.443832 4 GTAAGGT 2155 0.0 22.05481 4 >>END_MODULE