FastQCFastQC Report
Thu 26 May 2016
SRR938424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938424_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3167713
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73170.23098683498157818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59830.1888744340159604No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA57420.18126642154765915No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA56380.17798329583519718No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA53530.1689862686423928No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC49450.15610631392427282No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48350.15263377711301498No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46040.14534144980937352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42700.13479756530973608No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA40830.12889425273059774No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39850.12580053811693168No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36080.1138992074092571No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33780.1066384486220816No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA33310.10515472834818054No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31890.10067199901001134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40550.057.1855741
GTATCAA76700.044.9159051
ATCAACG103150.032.971353
TATCAAC104450.032.837472
TCAACGC104650.032.5436334
CAACGCA107950.031.6367845
AACGCAG111150.030.7259646
TGGTATC19850.027.7612132
ACGCAGA129950.026.13467
TAAGGTG23100.026.114275
CGCAGAG131350.025.6752328
GTGTAAG14600.025.3862921
GTGGTAT24850.025.0496941
AGGTAAG21100.024.0903022
TACATGG98200.023.752592
TAAGGTA13850.023.3208964
GTACATG101350.023.2549511
GCAGAGT150000.022.2296149
GTAAGGT24800.022.2173584
ACATGGG101850.021.8728183
Produced by FastQC (version 0.11.5)