FastQCFastQC Report
Thu 26 May 2016
SRR938423_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938423_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3345685
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90000.2690032086104938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72940.21801215595610465No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA49610.14828054643518443No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48610.1452916218950678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48380.14460416925084102No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA45220.13515916770407257No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA44000.1315126797651303No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA40360.1206329944391059No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC40240.12027432349429192No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG36060.10778061891660452No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA35050.10476180513108677No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA34280.10246033323519697No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63300.049.5166971
GTATCAA109100.039.3670581
ATCAACG138600.030.506263
TATCAAC140800.030.1309382
TCAACGC140700.030.0171414
CAACGCA144050.029.2530335
AACGCAG149750.028.147726
TAAGGTG19800.028.1041955
GTGTAAG13450.025.4612751
GTAAGGT21700.025.424284
ACGCAGA172200.024.2307747
CGCAGAG174650.023.7816668
AGGTAAG20350.023.6051832
TACATGG139450.023.0898232
GTACATG144200.022.7590311
TGGTATC32050.022.70462
GGTAAGG23600.022.3697663
ACATGGG139500.021.7518863
AAGGTAA22150.021.258441
GCAGAGT193450.020.8806089
Produced by FastQC (version 0.11.5)