FastQCFastQC Report
Thu 26 May 2016
SRR938423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938423_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3345685
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89840.26852498068407515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75950.2270088188218556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51710.15455728796942927No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48520.14502261868645733No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA48150.14391671660661418No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47860.14304992848998038No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC45970.13740086110916No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA44190.13208057542775245No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG41980.12547505219409477No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA36510.10912563495965699No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA36070.10781050816200569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.050.669851
GTATCAA104600.039.4851071
ATCAACG135100.030.0988123
TATCAAC136800.029.913162
TCAACGC136800.029.7942284
CAACGCA140250.029.0613215
AACGCAG144900.028.1484476
AGGTAAG19400.027.928552
TAAGGTG23200.026.6186685
GTAAGGT22500.025.9688994
TACATGG132500.024.7860572
GGTAAGG23150.024.6241473
GTACATG137900.024.5392341
ACGCAGA165750.024.4930177
CGCAGAG167450.024.2727138
TAAGGTA10450.024.092984
AAGGTAA22000.023.7637651
ACATGGG133800.023.36153
GAGTACT102150.021.07990312-13
GCAGAGT192900.020.947259
Produced by FastQC (version 0.11.5)