##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938423_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3345685 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.849695054973793 31.0 31.0 34.0 30.0 34.0 2 32.073587621070125 33.0 31.0 34.0 30.0 34.0 3 32.1361520884363 33.0 31.0 34.0 30.0 34.0 4 35.79733059149322 37.0 35.0 37.0 35.0 37.0 5 35.69112991808852 37.0 35.0 37.0 33.0 37.0 6 35.69331003964808 37.0 35.0 37.0 33.0 37.0 7 35.634475750107974 37.0 35.0 37.0 33.0 37.0 8 35.60791646553695 37.0 35.0 37.0 33.0 37.0 9 37.207178500068 39.0 37.0 39.0 33.0 39.0 10-11 37.111476424110464 39.0 37.0 39.0 33.0 39.0 12-13 37.00385526431807 39.0 37.0 39.0 33.0 39.0 14-15 38.07623281928812 40.0 37.0 41.0 33.0 41.0 16-17 37.98271938332509 40.0 37.0 41.0 33.0 41.0 18-19 38.21491563013254 40.0 37.5 41.0 33.0 41.0 20-21 38.18573326538511 40.0 37.5 41.0 33.0 41.0 22-23 38.0807870436099 40.0 37.0 41.0 33.0 41.0 24-25 37.95192270043354 40.0 37.0 41.0 33.0 41.0 26-27 37.7624041115646 40.0 37.0 41.0 32.0 41.0 28-29 37.654266017272995 40.0 37.0 41.0 32.0 41.0 30-31 37.40377949508098 39.0 36.0 41.0 31.5 41.0 32-33 37.20915104081824 39.0 36.0 41.0 31.0 41.0 34-35 37.1209904698141 39.0 36.0 41.0 31.0 41.0 36-37 37.07538994256781 39.0 36.0 41.0 31.0 41.0 38-39 36.90699572733237 39.0 35.5 41.0 30.5 41.0 40-41 36.733507637449435 39.0 35.0 41.0 30.0 41.0 42-43 36.5186728577257 39.0 35.0 41.0 30.0 41.0 44-45 36.22076958829059 39.0 35.0 40.0 30.0 41.0 46-47 35.84222080082255 38.0 35.0 40.0 28.5 41.0 48-49 35.477418824545644 38.0 34.5 40.0 27.5 41.0 50-51 35.095887239832805 38.0 34.0 40.0 26.5 41.0 52-53 34.60856775219425 38.0 33.0 40.0 25.5 41.0 54-55 34.265657257034064 37.5 33.0 40.0 24.0 41.0 56-57 33.92335411134043 37.0 33.0 40.0 24.0 41.0 58-59 33.43549542171483 36.5 32.0 39.0 23.0 41.0 60-61 33.03088679896643 36.0 31.5 39.0 22.0 40.0 62-63 32.549588798706395 35.5 31.0 39.0 20.5 40.0 64-65 32.02400330575054 35.0 31.0 38.0 19.0 40.0 66-67 31.522284524693745 35.0 30.0 38.0 18.0 40.0 68-69 31.028850893015928 34.0 30.0 37.0 15.0 39.5 70-71 30.46442851015562 34.0 29.0 37.0 10.0 39.0 72-73 29.968492849745267 34.0 29.0 36.0 8.0 39.0 74-75 29.395592980211823 34.0 28.0 36.0 7.0 38.5 76-77 28.17053712468448 31.5 26.5 34.5 6.0 36.5 78-79 28.859824669686475 33.0 28.0 35.0 2.0 37.0 80-81 28.513167109276576 33.0 27.5 35.0 2.0 36.5 82-83 28.09004105287856 33.0 26.5 35.0 2.0 36.0 84-85 27.554292020916492 33.0 26.0 35.0 2.0 36.0 86-87 26.970973657113568 32.0 25.5 35.0 2.0 35.0 88-89 26.23804871050323 32.0 24.0 34.0 2.0 35.0 90-91 25.655660201124732 31.5 22.5 34.0 2.0 35.0 92-93 24.860997075337337 31.0 19.5 34.0 2.0 35.0 94-95 24.07228893335744 31.0 13.0 34.0 2.0 35.0 96-97 23.03940329110481 30.5 2.0 34.0 2.0 35.0 98-99 21.708458507002305 30.0 2.0 34.0 2.0 35.0 100-101 17.738637080298954 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18505635190774683 1101 2 0.2809701995301026 1101 3 0.3167021207924847 1101 4 0.1876463392310157 1101 5 0.03767615868898133 1101 6 0.16942558659705753 1101 7 0.17848771452077727 1101 8 0.23498799200629605 1101 9 0.21738622114363437 1101 10-11 0.2774927650653254 1101 12-13 0.28237532730706505 1101 14-15 0.34415421054476525 1101 16-17 0.37290992336299666 1101 18-19 0.3647344965273973 1101 20-21 0.27665553861870507 1101 22-23 0.3556258332986104 1101 24-25 0.3877621547851575 1101 26-27 0.3264989169658179 1101 28-29 0.35038492419914746 1101 30-31 0.3294251962048449 1101 32-33 0.36586730344522067 1101 34-35 0.3937722900117606 1101 36-37 0.4719570051942057 1101 38-39 0.36425144874952053 1101 40-41 0.2916408544633313 1101 42-43 0.4385412641967079 1101 44-45 0.5177880057251443 1101 46-47 0.2636958793821762 1101 48-49 0.33698240559375137 1101 50-51 0.45375281619792673 1101 52-53 0.49311700437900896 1101 54-55 0.5558921557538952 1101 56-57 0.33359726822114055 1101 58-59 0.479312899819206 1101 60-61 0.48787170465521257 1101 62-63 0.5460270214882925 1101 64-65 0.4163304215889596 1101 66-67 0.41638944534769706 1101 68-69 0.44818438264416827 1101 70-71 0.42306336813288326 1101 72-73 0.4636739940600805 1101 74-75 0.5328290188101477 1101 76-77 0.4663723396375339 1101 78-79 0.6464179835846977 1101 80-81 0.8356525615792698 1101 82-83 0.6787519760583898 1101 84-85 0.7935960167871485 1101 86-87 0.7786151780190345 1101 88-89 0.9573296131429352 1101 90-91 0.8353644072334667 1101 92-93 1.0130640451716708 1101 94-95 1.0007125996859578 1101 96-97 1.1511538553029261 1101 98-99 1.2223482193824218 1101 100-101 1.2587556176507135 1102 1 0.23541197068441733 1102 2 0.27387560493550467 1102 3 0.1709073128266212 1102 4 0.19629676597354973 1102 5 0.09491300079425002 1102 6 0.08194336753588516 1102 7 0.12906559503286985 1102 8 0.1323741939977623 1102 9 0.19137270763012282 1102 10-11 0.26165439379931854 1102 12-13 0.3382830442345153 1102 14-15 0.3398734495205886 1102 16-17 0.32531924057494166 1102 18-19 0.3308929246924066 1102 20-21 0.24592563107959364 1102 22-23 0.30441672945792675 1102 24-25 0.314415782096674 1102 26-27 0.21582590843097194 1102 28-29 0.2018963039999946 1102 30-31 0.260412927357045 1102 32-33 0.2957126092773734 1102 34-35 0.358526024008917 1102 36-37 0.4307541246821103 1102 38-39 0.45676122826588283 1102 40-41 0.3275894675500908 1102 42-43 0.29753841924648583 1102 44-45 0.3942860498218721 1102 46-47 0.4425454526396422 1102 48-49 0.4592219148398371 1102 50-51 0.5169151561694818 1102 52-53 0.38108375402338623 1102 54-55 0.3458859324253112 1102 56-57 0.4580460591172937 1102 58-59 0.5869631487523463 1102 60-61 0.6100356449112567 1102 62-63 0.6215871210615518 1102 64-65 0.5341335865960701 1102 66-67 0.5981506473391676 1102 68-69 0.6567281237536982 1102 70-71 0.7358034108071365 1102 72-73 0.7885361917841216 1102 74-75 0.7446266717262198 1102 76-77 0.7591666141752285 1102 78-79 0.8980582751920956 1102 80-81 0.7980414307115602 1102 82-83 0.9187990955462979 1102 84-85 0.8341098859194389 1102 86-87 0.8689556829976972 1102 88-89 0.6846100185483408 1102 90-91 0.8244513560243654 1102 92-93 1.1632640807335441 1102 94-95 1.0489922938538108 1102 96-97 0.9577994811919694 1102 98-99 0.949313013123529 1102 100-101 1.2292036973093232 1103 1 0.2047380225595603 1103 2 0.21223794923445638 1103 3 0.2552043047074761 1103 4 0.10097609744659763 1103 5 0.03795961445391782 1103 6 0.07504377265279771 1103 7 0.17414567286238025 1103 8 0.1457602821821311 1103 9 0.236553959626292 1103 10-11 0.21498310793134578 1103 12-13 0.24709733132961276 1103 14-15 0.44433262474625934 1103 16-17 0.3550460598188323 1103 18-19 0.4045272835654785 1103 20-21 0.3380770687403398 1103 22-23 0.2612588557333737 1103 24-25 0.421342167646948 1103 26-27 0.36768281685101556 1103 28-29 0.3840569765613182 1103 30-31 0.28921904067211557 1103 32-33 0.3546539681840528 1103 34-35 0.47516434159704346 1103 36-37 0.49820897978137424 1103 38-39 0.5639292025972722 1103 40-41 0.5272066761937069 1103 42-43 0.6414759539167605 1103 44-45 0.7046581879482474 1103 46-47 0.5982501837664032 1103 48-49 0.690252073776783 1103 50-51 0.6598443636700253 1103 52-53 0.6497827466942994 1103 54-55 0.6826322741859698 1103 56-57 0.7426038134920532 1103 58-59 0.6908224231560212 1103 60-61 0.7693560654932199 1103 62-63 0.7801781000660739 1103 64-65 0.9198768017282788 1103 66-67 0.801375245584218 1103 68-69 0.6594402848254894 1103 70-71 0.6277754560592008 1103 72-73 0.48626208438611584 1103 74-75 0.48808249969441775 1103 76-77 0.5112628525554292 1103 78-79 0.8481733699995395 1103 80-81 0.8568248297433136 1103 82-83 0.9886932671578563 1103 84-85 0.9179521718882739 1103 86-87 0.8517232429667665 1103 88-89 0.8739973947859632 1103 90-91 0.9355099655992447 1103 92-93 1.3134153166763234 1103 94-95 1.0238610195846185 1103 96-97 1.1257492807347411 1103 98-99 1.2441578583890411 1103 100-101 1.5098005303372766 1104 1 0.10844902424110003 1104 2 0.16592676626633107 1104 3 0.1678835988807421 1104 4 0.1251353112538922 1104 5 0.0029382428069979483 1104 6 0.020462216447242554 1104 7 0.10688157448242919 1104 8 0.12551771664619338 1104 9 0.19318532533257127 1104 10-11 0.17475581201057366 1104 12-13 0.16833671242099513 1104 14-15 0.3005530882360148 1104 16-17 0.3517756534999137 1104 18-19 0.32246553264827327 1104 20-21 0.31182630260303057 1104 22-23 0.2142192784386623 1104 24-25 0.15834089030033738 1104 26-27 0.10871492534708693 1104 28-29 0.17528047653113532 1104 30-31 0.2412770904719892 1104 32-33 0.30075399098021194 1104 34-35 0.3606641819036511 1104 36-37 0.22265919043028504 1104 38-39 0.2295524489022185 1104 40-41 0.247542200724844 1104 42-43 0.33977088364665775 1104 44-45 0.3528484899381681 1104 46-47 0.2719853128834657 1104 48-49 0.13106366906060174 1104 50-51 0.3258497012185444 1104 52-53 0.38559454547942806 1104 54-55 0.29039593919836193 1104 56-57 0.44230841580940705 1104 58-59 0.5079853222416233 1104 60-61 0.359711446939869 1104 62-63 0.43530358618858855 1104 64-65 0.3977824244392707 1104 66-67 0.4139734700503723 1104 68-69 0.43827341235184036 1104 70-71 0.48094911404828977 1104 72-73 0.3434696795761063 1104 74-75 0.419754079464024 1104 76-77 0.3012333659180513 1104 78-79 0.560985411181786 1104 80-81 0.5152258849025948 1104 82-83 0.759142642148209 1104 84-85 0.8606183357162465 1104 86-87 1.0254942604913388 1104 88-89 1.0209506494334626 1104 90-91 1.117251166874464 1104 92-93 1.345398604307075 1104 94-95 1.422452559985242 1104 96-97 1.4588194658287037 1104 98-99 1.525702315003464 1104 100-101 1.6653808869878475 1105 1 0.15437718835043768 1105 2 0.17518112714439127 1105 3 0.14591718519662322 1105 4 0.17125451222995025 1105 5 -0.004745810098533809 1105 6 0.06701002577813142 1105 7 0.14576530123113685 1105 8 0.08564866167957064 1105 9 0.1612256488065924 1105 10-11 0.14994438084677597 1105 12-13 0.23147091148668153 1105 14-15 0.4132379927062786 1105 16-17 0.2689207601301291 1105 18-19 0.3081740345047521 1105 20-21 0.14866244949116236 1105 22-23 0.1244232847116038 1105 24-25 0.3337707609303209 1105 26-27 0.14484416046862236 1105 28-29 0.14469460753193886 1105 30-31 0.25292986834396913 1105 32-33 0.25628394307404534 1105 34-35 0.21705673892645905 1105 36-37 0.2416842204046148 1105 38-39 0.19272331046718705 1105 40-41 0.2765506967786351 1105 42-43 0.3846048247560603 1105 44-45 0.45144687467813327 1105 46-47 0.5209340449186541 1105 48-49 0.5707092071251694 1105 50-51 0.6194154325958365 1105 52-53 0.5781854236385939 1105 54-55 0.6272237965183152 1105 56-57 0.37963061399187836 1105 58-59 0.38385789349234756 1105 60-61 0.5163784451892681 1105 62-63 0.6026799529311404 1105 64-65 0.4834644373574264 1105 66-67 0.35983222786611435 1105 68-69 0.4844611906646712 1105 70-71 0.46391518183133584 1105 72-73 0.48857706339592255 1105 74-75 0.4102664802812228 1105 76-77 0.44690651276624394 1105 78-79 0.7108348989995115 1105 80-81 0.6808143425078583 1105 82-83 0.7141679667790015 1105 84-85 0.7330930318093749 1105 86-87 0.7493469842550446 1105 88-89 0.7238244543226493 1105 90-91 0.9256579570858428 1105 92-93 1.011871879116054 1105 94-95 1.192956524282824 1105 96-97 1.4504463425680996 1105 98-99 1.4963576204401399 1105 100-101 1.546677002420843 1106 1 -0.006184504681208125 1106 2 0.13349736503194976 1106 3 0.13600815829472168 1106 4 0.1214409159053389 1106 5 0.07082112553648301 1106 6 0.04073115980136066 1106 7 0.12742125475178767 1106 8 0.19474293229921358 1106 9 -0.05898142136324935 1106 10-11 0.13814219161665164 1106 12-13 0.09240579176791641 1106 14-15 0.13056606317497454 1106 16-17 0.15205439333024628 1106 18-19 0.03200597234317826 1106 20-21 0.12199545357887587 1106 22-23 0.2718822683050419 1106 24-25 0.1780223856308254 1106 26-27 0.08725973429309164 1106 28-29 0.15583197387088177 1106 30-31 0.14020566473847396 1106 32-33 0.16219884067865564 1106 34-35 0.27793362132499055 1106 36-37 0.35900899841708167 1106 38-39 0.21630932715233797 1106 40-41 0.2662851981741241 1106 42-43 0.2397912031048719 1106 44-45 0.167966393878217 1106 46-47 -0.40254294081234576 1106 48-49 -0.14647260230746184 1106 50-51 0.21609397743149117 1106 52-53 0.24601774644113306 1106 54-55 0.49940114847175465 1106 56-57 0.4066122927393252 1106 58-59 0.08435328152097554 1106 60-61 0.25114197085269296 1106 62-63 -0.008404987374099449 1106 64-65 0.22456784074410052 1106 66-67 0.11099218552006107 1106 68-69 -0.10384121890675146 1106 70-71 -0.03900623990191576 1106 72-73 0.2663451326489401 1106 74-75 0.4331515836896749 1106 76-77 0.3403094435588123 1106 78-79 0.5632321870286496 1106 80-81 0.06778432037242155 1106 82-83 0.441078984350586 1106 84-85 0.5268542071655915 1106 86-87 0.7602697077748282 1106 88-89 0.9372452656851706 1106 90-91 0.7661891062543695 1106 92-93 0.8177720873005931 1106 94-95 1.128623829179464 1106 96-97 1.408494751805211 1106 98-99 1.4954243357338548 1106 100-101 1.3506409930803969 1107 1 0.16918244820271866 1107 2 0.1808832352263252 1107 3 0.14001079154212448 1107 4 0.09951113016649771 1107 5 0.11618537262506123 1107 6 0.0964257655839873 1107 7 0.1258574820712255 1107 8 0.18226242955930871 1107 9 0.1552044093289382 1107 10-11 0.20949587204503928 1107 12-13 0.20107206896666696 1107 14-15 0.3023966074910973 1107 16-17 0.2685922526833906 1107 18-19 0.23855357186533865 1107 20-21 0.26295914715093716 1107 22-23 0.2675495245539281 1107 24-25 0.2554319102734439 1107 26-27 0.22927472221247314 1107 28-29 0.20534651010120086 1107 30-31 0.18339685790397198 1107 32-33 0.2359142777878276 1107 34-35 0.34151647099089644 1107 36-37 0.3832972865927857 1107 38-39 0.40875854402504785 1107 40-41 0.4205408727511184 1107 42-43 0.30815414658672324 1107 44-45 0.21898916369278254 1107 46-47 0.3099004809027832 1107 48-49 0.4065942665909361 1107 50-51 0.48033441867573146 1107 52-53 0.3075602285562482 1107 54-55 0.25196757540044246 1107 56-57 0.34275317945918005 1107 58-59 0.4292722872947792 1107 60-61 0.4668474032879075 1107 62-63 0.5963814934379243 1107 64-65 0.4290891744391274 1107 66-67 0.418742345325132 1107 68-69 0.537250518316263 1107 70-71 0.7194528417556576 1107 72-73 0.5866713831854575 1107 74-75 0.6107719920165842 1107 76-77 0.6122567588249304 1107 78-79 0.8749202423219806 1107 80-81 0.8099891912553048 1107 82-83 1.0352963317443376 1107 84-85 1.1582886067280853 1107 86-87 1.314233966977877 1107 88-89 1.2473556635238658 1107 90-91 1.343474752678251 1107 92-93 1.566821457565343 1107 94-95 1.6030544102555275 1107 96-97 1.8746997323778096 1107 98-99 1.826827902419904 1107 100-101 1.784256586514772 1108 1 0.19748353865416846 1108 2 0.1833918274152282 1108 3 0.2249061388313791 1108 4 0.21222002937539486 1108 5 0.11448670330080546 1108 6 0.22760415914410714 1108 7 0.17547614269402345 1108 8 0.15213238147107688 1108 9 0.23715775977380815 1108 10-11 0.2157273568413416 1108 12-13 0.28283178028380007 1108 14-15 0.44008899962121717 1108 16-17 0.4186789252733689 1108 18-19 0.2943326926377594 1108 20-21 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2316 68-69 -0.7610429036953086 2316 70-71 -0.6501258261608847 2316 72-73 -0.6453477480295291 2316 74-75 -0.6066875438366104 2316 76-77 -0.6124376731579382 2316 78-79 -0.7998035669131696 2316 80-81 -0.8146261907943568 2316 82-83 -0.8234564760981264 2316 84-85 -1.0657437403711683 2316 86-87 -1.2259629458111654 2316 88-89 -1.1860530700943173 2316 90-91 -0.9757760740939929 2316 92-93 -1.3332504936809606 2316 94-95 -1.2539296716460662 2316 96-97 -1.6583181529052347 2316 98-99 -1.5634104822176944 2316 100-101 -1.8799438725723192 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 19.0 4 53.0 5 91.0 6 241.0 7 619.0 8 1462.0 9 2581.0 10 4258.0 11 6675.0 12 9400.0 13 13024.0 14 16837.0 15 22451.0 16 27935.0 17 34598.0 18 33654.0 19 29554.0 20 29586.0 21 31362.0 22 34492.0 23 38075.0 24 43955.0 25 51888.0 26 61313.0 27 73726.0 28 87540.0 29 104067.0 30 124203.0 31 147556.0 32 178391.0 33 219893.0 34 282149.0 35 369562.0 36 482668.0 37 525384.0 38 246114.0 39 10297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94635822476534 18.647322693530395 14.897407878108936 23.50891120359533 2 16.280609501617533 20.107997121990635 38.52938027702336 25.082013099368478 3 19.628446790418106 23.39272824548635 29.580459606926535 27.39836535716901 4 13.66187003913101 16.590239843332487 35.40630256772606 34.34158754981044 5 14.335239822273685 37.12163032615255 33.54225366011786 15.000876191455909 6 33.67313420121739 35.32042018301185 16.48672842781075 14.519717187960012 7 29.897614389878306 32.12481151094619 19.497651452542602 18.4799226466329 8 27.576385702778357 34.253912128607446 18.95644688606369 19.21325528255051 9 26.789611096083465 16.284707018144267 19.205693303464013 37.719988582308254 10-11 25.751542658678268 26.032785513280537 26.591564956055336 21.624106871985855 12-13 26.558955789322663 23.13178018851147 26.994920322744072 23.31434369942179 14-15 23.07790482367587 24.779753622950157 25.088270413980997 27.054071139392978 16-17 22.992272135601528 27.846823595168104 26.043635309361164 23.117268959869204 18-19 23.022385550343202 26.147874052697727 28.255245188952337 22.57449520800673 20-21 23.488433609260884 25.463798893201243 28.423835477637617 22.62393201990026 22-23 23.197954977829653 25.350399096149218 28.50280286398749 22.94884306203363 24-25 23.082022967465125 25.67200168944796 28.52083779521063 22.725137547876287 26-27 22.550008608259624 25.99420915201469 28.22612463325227 23.22965760647341 28-29 22.673168381389562 26.453653762320172 28.106246813989916 22.76693104230035 30-31 22.827961075785588 25.905767509522498 27.964632664794475 23.301638749897442 32-33 22.435217069928832 25.894565906470834 28.077669276106782 23.592547747493555 34-35 23.26814404995211 26.066247290215124 27.821972663606438 22.84363599622633 36-37 23.517388251694772 25.480920076779196 27.833504961932533 23.168186709593495 38-39 22.843825735338953 25.87902312696456 27.871486507975412 23.405664629721073 40-41 23.317282149679965 25.750649761339456 28.013034232644973 22.919033856335606 42-43 23.048955597188463 25.859176887917545 27.654526303456077 23.437341211437918 44-45 23.03243947881852 26.002967060132438 27.969174977334543 22.9954184837145 46-47 23.498072890902762 26.285917532583014 27.22246714798315 22.99354242853108 48-49 23.187239683353333 26.406117132963804 27.058509692335054 23.34813349134781 50-51 23.26683585074479 26.48084514127309 27.20750176609108 23.04481724189104 52-53 23.731507897485866 26.07772399374119 27.094272174457544 23.096495934315396 54-55 23.09881269105129 26.372886685419473 27.246039837603224 23.282260785926013 56-57 23.12351581215805 26.631975215837713 26.926503840020803 23.318005131983437 58-59 23.081851333874255 26.122777897694355 27.10286171439778 23.69250905403361 60-61 22.612798275988325 26.392562360174377 27.47866281493924 23.515976548898056 62-63 22.863760116985777 26.33693971717278 27.552980781762464 23.24631938407898 64-65 22.602070900890464 26.421646183107693 27.21660009042909 23.759682825572746 66-67 22.505354627996272 26.56279587579684 27.157256356019282 23.774593140187605 68-69 23.16350334567512 26.01308555293434 27.331834741122158 23.491576360268386 70-71 22.883716551107288 26.13782090799112 27.005775353758168 23.97268718714343 72-73 22.834892432261576 26.28551872450539 27.01495135895292 23.864637484280113 74-75 22.81490747032745 26.655185598968227 26.757295450692947 23.77261148001137 76-77 22.89268551830082 26.249902997602113 27.059325395609047 23.798086088488017 78-79 22.873317139261196 26.496353343910457 26.962158403020712 23.66817111380763 80-81 23.05943660013768 26.433348057758614 26.778258377039464 23.728956965064246 82-83 22.991814693425876 26.53883569242766 26.804946735183783 23.664402878962683 84-85 22.73172286197117 26.663924387224604 26.800943694884744 23.803409055919484 86-87 23.058675053215545 26.521803203664064 26.52308856722915 23.896433175891243 88-89 22.735359297164894 26.66309481501159 26.299298433291334 24.302247454532182 90-91 22.583354186267677 26.6471501139213 26.639810478965742 24.129685220845282 92-93 22.692145445130706 26.31982789058639 26.734031939857374 24.253994724425525 94-95 22.476725129833856 26.00366454289409 26.972150879189826 24.54745944808223 96-97 22.767977681795145 25.585695896518164 26.99673031122593 24.649596110460763 98-99 22.296427814177793 26.012070470590455 26.582204474286176 25.109297240945576 100-101 22.418383564178644 25.523334814557728 27.186476286733356 24.87180533453027 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 394.0 1 267.5 2 235.5 3 355.0 4 331.0 5 300.5 6 551.0 7 1238.5 8 1955.5 9 2270.5 10 2064.5 11 1775.0 12 1833.0 13 1996.0 14 2177.0 15 2402.5 16 2638.0 17 2913.5 18 3232.5 19 3646.0 20 4161.0 21 4907.0 22 5983.5 23 7263.0 24 8897.0 25 11115.0 26 14415.5 27 18904.5 28 22671.0 29 26429.5 30 31383.0 31 38945.0 32 50708.0 33 55370.0 34 55040.0 35 59135.0 36 65657.5 37 72666.0 38 77780.5 39 81866.5 40 87374.5 41 94313.5 42 98620.0 43 101464.5 44 105472.5 45 112916.5 46 117500.5 47 120232.5 48 123353.0 49 123191.0 50 121801.5 51 121071.0 52 122886.0 53 127987.0 54 133124.5 55 127058.5 56 116642.0 57 107382.5 58 100532.5 59 90592.0 60 74448.0 61 62914.0 62 53705.0 63 40674.0 64 27931.0 65 20579.0 66 15527.0 67 12192.0 68 9533.0 69 7452.0 70 5814.0 71 3979.0 72 3015.0 73 2575.0 74 1878.5 75 1318.0 76 1032.0 77 698.5 78 459.0 79 323.0 80 186.5 81 109.5 82 65.5 83 34.5 84 18.0 85 11.5 86 8.0 87 5.0 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.690032086104938E-4 2 0.051020941899790326 3 0.0 4 2.988924540116598E-5 5 0.049825372083743685 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006381353893148937 26-27 0.001823243969471125 28-29 1.0461235890408094E-4 30-31 0.0016588531197647119 32-33 3.5867094481399175E-4 34-35 0.011985587405867557 36-37 0.022969885090796054 38-39 0.004573054546378395 40-41 0.0010012897209390602 42-43 0.001225459061447805 44-45 0.00854832418473347 46-47 0.0 48-49 0.0 50-51 5.828402853227366E-4 52-53 0.0 54-55 0.001554240760860631 56-57 0.0 58-59 0.022925051222694306 60-61 0.0 62-63 0.00959444777377428 64-65 0.015617130722109225 66-67 0.0064112431385501024 68-69 0.016304583366336042 70-71 7.472311350291495E-5 72-73 7.472311350291495E-5 74-75 0.022656048014083812 76-77 0.05067721557767692 78-79 0.03124920606691903 80-81 0.026975043974552294 82-83 0.036360267030518416 84-85 0.016020635535024964 86-87 0.00980367249158244 88-89 0.0861109160007592 90-91 0.024867852173770095 92-93 0.024987409155374757 94-95 0.02778205360038378 96-97 0.03975269638355075 98-99 0.05445820512092441 100-101 0.014331893169859087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3345685.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.01572483609178 #Duplication Level Percentage of deduplicated Percentage of total 1 71.18812302177807 31.33396834524434 2 14.623588789477369 12.873357205475846 3 5.398321843678847 7.128331465440954 4 2.6916005830500134 4.738910025287744 5 1.5032413470280115 3.3083128746510453 6 0.9479863887799311 2.5035784822138663 7 0.6408369882914137 1.9744833178997243 8 0.4532075320452687 1.5958606419319035 9 0.34755942035331333 1.3768271829416698 >10 1.9537068383110663 15.7579049172029 >50 0.1585950508857709 4.776445124232543 >100 0.08359637337668907 6.660839588573557 >500 0.005360774737634633 1.5288908670024832 >1k 0.004071474482531811 3.792657697587791 >5k 2.0357372412659058E-4 0.649632264313549 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8984 0.26852498068407515 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7595 0.2270088188218556 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5171 0.15455728796942927 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4852 0.14502261868645733 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4815 0.14391671660661418 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4786 0.14304992848998038 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4597 0.13740086110916 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4419 0.13208057542775245 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4198 0.12547505219409477 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3651 0.10912563495965699 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3607 0.10781050816200569 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.977849080233196E-5 2 0.0 0.0 0.0 0.0 5.977849080233196E-5 3 0.0 0.0 0.0 0.0 5.977849080233196E-5 4 0.0 0.0 0.0 2.988924540116598E-5 5.977849080233196E-5 5 0.0 0.0 0.0 2.988924540116598E-5 5.977849080233196E-5 6 0.0 0.0 0.0 2.988924540116598E-5 8.966773620349794E-5 7 0.0 0.0 0.0 2.988924540116598E-5 8.966773620349794E-5 8 0.0 2.988924540116598E-5 0.0 2.988924540116598E-5 8.966773620349794E-5 9 0.0 2.988924540116598E-5 0.0 2.988924540116598E-5 8.966773620349794E-5 10-11 0.0 2.988924540116598E-5 0.0 2.988924540116598E-5 8.966773620349794E-5 12-13 0.0 5.977849080233196E-5 0.0 5.977849080233196E-5 1.643908497064129E-4 14-15 0.0 5.977849080233196E-5 0.0 5.977849080233196E-5 1.7933547240699588E-4 16-17 0.0 5.977849080233196E-5 0.0 7.472311350291495E-5 1.7933547240699588E-4 18-19 0.0 5.977849080233196E-5 0.0 1.494462270058299E-4 1.7933547240699588E-4 20-21 0.0 5.977849080233196E-5 0.0 2.690032086104938E-4 2.690032086104938E-4 22-23 0.0 8.966773620349794E-5 0.0 4.931725491192386E-4 2.690032086104938E-4 24-25 0.0 1.0461235890408093E-4 0.0 7.771203804303155E-4 2.690032086104938E-4 26-27 0.0 1.494462270058299E-4 0.0 0.0012404036841483883 2.988924540116598E-4 28-29 0.0 1.7933547240699588E-4 0.0 0.0038258234113492454 2.988924540116598E-4 30-31 0.0 1.7933547240699588E-4 0.0 0.012837430899800788 3.1383707671224276E-4 32-33 0.0 1.7933547240699588E-4 0.0 0.03150326465282895 4.333940583169067E-4 34-35 0.0 1.9428009510757887E-4 0.0 0.06154195628100075 4.7822792641865567E-4 36-37 0.0 2.0922471780816186E-4 0.0 0.11151677459175027 4.7822792641865567E-4 38-39 0.0 2.0922471780816186E-4 0.0 0.1845959795976011 5.380064172209876E-4 40-41 0.0 2.0922471780816186E-4 0.0 0.26269657783084777 5.380064172209876E-4 42-43 0.0 2.0922471780816186E-4 0.0 0.3447126672116473 5.678956626221537E-4 44-45 0.0 2.0922471780816186E-4 0.0 0.44777078535486753 5.678956626221537E-4 46-47 0.0 2.0922471780816186E-4 0.0 0.5697786850824271 5.678956626221537E-4 48-49 0.0 2.0922471780816186E-4 0.0 0.7000957950315108 6.575633988256515E-4 50-51 0.0 2.0922471780816186E-4 0.0 0.8450735798498663 7.771203804303155E-4 52-53 0.0 2.0922471780816186E-4 0.0 0.9831917828486543 8.667881166338135E-4 54-55 0.0 2.0922471780816186E-4 0.0 1.1323540620231731 9.265666074361454E-4 56-57 0.0 2.0922471780816186E-4 0.0 1.3116895344301691 9.564558528373113E-4 58-59 0.0 2.0922471780816186E-4 0.0 1.5060592972739513 9.564558528373113E-4 60-61 0.0 2.0922471780816186E-4 0.0 1.7197524572695875 9.564558528373113E-4 62-63 0.0 2.0922471780816186E-4 0.0 1.9282149993200197 0.0010162343436396434 64-65 0.0 2.0922471780816186E-4 0.0 2.1396664659105684 0.0010461235890408093 66-67 0.0 2.0922471780816186E-4 0.0 2.3877770919856474 0.0010461235890408093 68-69 0.0 2.0922471780816186E-4 0.0 2.6677944875264705 0.0010461235890408093 70-71 0.0 2.0922471780816186E-4 0.0 2.9639072417158223 0.0010760128344419753 72-73 0.0 2.0922471780816186E-4 0.0 3.2761452437991023 0.0011357913252443073 74-75 0.0 2.0922471780816186E-4 0.0 3.5823755075567485 0.0012105144387472221 76-77 0.0 2.0922471780816186E-4 0.0 3.914220854623194 0.001225459061447805 78-79 0.0 2.0922471780816186E-4 0.0 4.293455600273187 0.0012553483068489712 80-81 0.0 2.0922471780816186E-4 0.0 4.686783125129831 0.0012553483068489712 82-83 0.0 2.0922471780816186E-4 0.0 5.1028264764913605 0.0012852375522501372 84-85 0.0 2.0922471780816186E-4 0.0 5.5220231432427145 0.0012852375522501372 86-87 0.0 2.0922471780816186E-4 0.0 5.955043585992106 0.001359960665753052 88-89 0.0 2.0922471780816186E-4 0.0 6.413036493274173 0.001434683779255967 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6050 0.0 50.66985 1 GTATCAA 10460 0.0 39.485107 1 ATCAACG 13510 0.0 30.098812 3 TATCAAC 13680 0.0 29.91316 2 TCAACGC 13680 0.0 29.794228 4 CAACGCA 14025 0.0 29.061321 5 AACGCAG 14490 0.0 28.148447 6 AGGTAAG 1940 0.0 27.92855 2 TAAGGTG 2320 0.0 26.618668 5 GTAAGGT 2250 0.0 25.968899 4 TACATGG 13250 0.0 24.786057 2 GGTAAGG 2315 0.0 24.624147 3 GTACATG 13790 0.0 24.539234 1 ACGCAGA 16575 0.0 24.493017 7 CGCAGAG 16745 0.0 24.272713 8 TAAGGTA 1045 0.0 24.09298 4 AAGGTAA 2200 0.0 23.763765 1 ACATGGG 13380 0.0 23.3615 3 GAGTACT 10215 0.0 21.079903 12-13 GCAGAGT 19290 0.0 20.94725 9 >>END_MODULE