FastQCFastQC Report
Thu 26 May 2016
SRR938422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3318771
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91680.27624683956802076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72530.2185447564776238No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA49240.14836817605071276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49130.14803672805384885No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48840.1471629106075713No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA44570.1342967020020363No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA43560.13125340675810412No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC40280.12137023012434422No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39920.12028549122551692No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG35920.10823283679410241No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA35730.1076603357086102No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA34890.10512927827801316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62200.051.1431771
GTATCAA109200.040.5130271
ATCAACG137750.031.9493543
TATCAAC138100.031.7995532
TCAACGC139650.031.5487044
CAACGCA142200.030.8469415
AACGCAG146750.030.0155356
ACGCAGA169700.025.7283927
CGCAGAG169500.025.6466338
TAAGGTG19700.025.3298745
TACATGG137300.024.1270922
GTACATG143400.023.7110441
ACATGGG138000.022.9715063
GAGTACT99600.022.60526512-13
GCAGAGT191300.022.0528099
GTAAGGT21050.021.6751354
GTACTTT105500.021.11851314-15
GTGTAAG13500.020.812291
AGGTAAG19400.020.5788122
CAGAGTA178050.020.47715210-11
Produced by FastQC (version 0.11.5)