##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938422_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3318771 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.124386708212167 31.0 31.0 34.0 28.0 34.0 2 31.21261454918101 31.0 31.0 34.0 28.0 34.0 3 31.28439292738185 31.0 31.0 34.0 27.0 34.0 4 34.91779697966506 37.0 35.0 37.0 32.0 37.0 5 34.94394491213765 37.0 35.0 37.0 32.0 37.0 6 34.98010438201371 37.0 35.0 37.0 32.0 37.0 7 34.900613510242195 37.0 35.0 37.0 32.0 37.0 8 34.85189366786681 37.0 35.0 37.0 32.0 37.0 9 36.36059583502447 39.0 37.0 39.0 32.0 39.0 10-11 36.21789677564375 39.0 35.0 39.0 32.0 39.0 12-13 36.07954043831286 39.0 35.0 39.0 30.0 39.0 14-15 37.09534433077787 39.0 36.0 41.0 31.0 41.0 16-17 36.9556023901619 39.0 36.0 41.0 31.0 41.0 18-19 36.94355802193041 39.0 36.0 41.0 31.0 41.0 20-21 37.015903025547715 39.0 36.0 41.0 31.0 41.0 22-23 36.938643100111456 39.0 36.0 41.0 30.5 41.0 24-25 36.82179020486801 39.0 36.0 41.0 30.0 41.0 26-27 36.643938373572624 39.0 36.0 41.0 30.0 41.0 28-29 36.47011378609732 39.0 36.0 41.0 30.0 41.0 30-31 36.243426105627655 39.0 35.0 40.5 29.5 41.0 32-33 35.96182758617573 38.5 35.0 40.0 28.5 41.0 34-35 35.71838566143913 38.0 35.0 40.0 27.0 41.0 36-37 35.438350973899674 38.0 34.5 40.0 26.5 41.0 38-39 35.16969067766351 38.0 34.0 40.0 25.5 41.0 40-41 35.10138014945895 38.0 34.0 40.0 25.0 41.0 42-43 34.91861339634461 38.0 34.0 40.0 25.0 41.0 44-45 34.63502724351876 38.0 33.0 40.0 24.0 41.0 46-47 34.27465453325945 38.0 33.0 40.0 23.5 41.0 48-49 33.90349620386583 38.0 33.0 40.0 22.5 41.0 50-51 33.13827422862258 37.0 32.0 39.5 20.5 40.5 52-53 33.17702667644137 37.0 32.0 39.5 20.0 40.5 54-55 33.29600987835558 37.0 32.5 40.0 19.5 41.0 56-57 32.99484764088875 37.0 32.0 40.0 18.0 41.0 58-59 32.66052493528478 36.5 31.5 39.5 16.0 41.0 60-61 32.2180501456714 36.0 31.0 39.0 12.5 41.0 62-63 31.698351739243233 35.5 30.5 39.0 8.0 40.0 64-65 31.198425561751627 35.0 30.0 38.5 7.5 40.0 66-67 30.672318306987737 35.0 29.0 38.0 4.0 40.0 68-69 30.130781545337115 34.5 29.0 37.5 2.0 40.0 70-71 29.51363908507095 34.0 28.0 37.0 2.0 39.0 72-73 28.980697975244453 34.0 27.5 36.0 2.0 39.0 74-75 28.397687577720788 34.0 26.0 36.0 2.0 38.5 76-77 27.691071785308477 33.0 25.5 35.0 2.0 37.5 78-79 26.941846093026605 32.5 24.5 35.0 2.0 37.0 80-81 26.319598881634192 32.0 23.5 35.0 2.0 36.0 82-83 25.68402505023697 32.0 20.5 35.0 2.0 36.0 84-85 25.050324050680207 31.0 19.0 34.0 2.0 35.5 86-87 24.408876960778553 31.0 15.5 34.0 2.0 35.0 88-89 23.630032171547843 30.5 7.0 34.0 2.0 35.0 90-91 22.830279190700413 30.0 2.0 34.0 2.0 35.0 92-93 22.025476448962582 29.0 2.0 34.0 2.0 35.0 94-95 21.082751717427925 29.0 2.0 34.0 2.0 35.0 96-97 19.87244359433055 27.5 2.0 34.0 2.0 35.0 98-99 18.41494426701933 25.0 2.0 33.0 2.0 35.0 100-101 14.950151125220751 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46488172475346445 1101 2 0.559406817989224 1101 3 0.5670671470155924 1101 4 0.5188300219475721 1101 5 0.4406723180091916 1101 6 0.4484902999773155 1101 7 0.21298254988533927 1101 8 0.3929618553312437 1101 9 0.5396386296602529 1101 10-11 0.5215378728788878 1101 12-13 0.5087887840552625 1101 14-15 0.6104106261336426 1101 16-17 0.6537675630106108 1101 18-19 0.528433395192458 1101 20-21 0.686952721477418 1101 22-23 0.7479455334991343 1101 24-25 0.7594112569403606 1101 26-27 0.7980618915051352 1101 28-29 0.6628603507116537 1101 30-31 0.5315360144120191 1101 32-33 0.6450734696058404 1101 34-35 0.6682362836231377 1101 36-37 0.6703652762016432 1101 38-39 0.5995437326539701 1101 40-41 0.5879766171038554 1101 42-43 0.6584957233178486 1101 44-45 0.6065392971027634 1101 46-47 0.6895801154477255 1101 48-49 0.7819386422963888 1101 50-51 0.6607013658603833 1101 52-53 0.7285023111143332 1101 54-55 0.7504311177152729 1101 56-57 0.5506886537593729 1101 58-59 0.7674339726656925 1101 60-61 0.7736360922093084 1101 62-63 0.8028449486635054 1101 64-65 0.7998555638632752 1101 66-67 0.756317517174331 1101 68-69 0.6633925725819623 1101 70-71 0.8013582635099397 1101 72-73 0.7576181522831718 1101 74-75 0.660209456387161 1101 76-77 0.8094088537394342 1101 78-79 0.5925983375536994 1101 80-81 0.7738534797250267 1101 82-83 0.8154901251815865 1101 84-85 0.9514727223153514 1101 86-87 1.085405877890988 1101 88-89 1.02798703146917 1101 90-91 0.88448503230439 1101 92-93 0.9059354033749933 1101 94-95 0.9122412384175895 1101 96-97 0.9396379866635662 1101 98-99 0.9956976639431119 1101 100-101 0.9179974491704428 1102 1 0.5210945482597573 1102 2 0.606894363213641 1102 3 0.5971718711610983 1102 4 0.5347994138308749 1102 5 0.6218834654667944 1102 6 0.6078078591880711 1102 7 0.5562627505488251 1102 8 0.5159936540204555 1102 9 0.5611712316969388 1102 10-11 0.6818325684737729 1102 12-13 0.7209099103599854 1102 14-15 0.7001666165628322 1102 16-17 0.6476894591418301 1102 18-19 0.7365633294219975 1102 20-21 0.6295979886626952 1102 22-23 0.749571531904003 1102 24-25 0.5297277989629521 1102 26-27 0.542149658661522 1102 28-29 0.4532691332067742 1102 30-31 0.5244180090255455 1102 32-33 0.6091839501116638 1102 34-35 0.519163600904939 1102 36-37 0.5948382455819612 1102 38-39 0.6948390639914521 1102 40-41 0.5897784272364319 1102 42-43 0.6678867396968897 1102 44-45 0.5856335372747523 1102 46-47 0.5704537413767738 1102 48-49 0.5344425434441931 1102 50-51 0.7467313844703085 1102 52-53 0.8066147335248388 1102 54-55 0.6933967991735486 1102 56-57 0.6299142614715052 1102 58-59 0.7567070298940806 1102 60-61 0.7829231935697933 1102 62-63 0.538263787450731 1102 64-65 0.4833648630437466 1102 66-67 0.5733758783454803 1102 68-69 0.47563177362689046 1102 70-71 0.6071745570149609 1102 72-73 0.7239230428776509 1102 74-75 0.48848052547504395 1102 76-77 0.5552060749662999 1102 78-79 0.7037505343333699 1102 80-81 0.6294452405008641 1102 82-83 0.45703844739906074 1102 84-85 0.3860799489749276 1102 86-87 0.5164379771130676 1102 88-89 0.7232202912104846 1102 90-91 0.8674037843994498 1102 92-93 0.5839371487774194 1102 94-95 0.5146438253120458 1102 96-97 0.5890472083990304 1102 98-99 0.5160298683516977 1102 100-101 0.4217319746174848 1103 1 0.6081887543632511 1103 2 0.6311282595012031 1103 3 0.5736495577523044 1103 4 0.5065402037140743 1103 5 0.4167254724450302 1103 6 0.5132534994324729 1103 7 0.33785507558800276 1103 8 0.4004067564934175 1103 9 0.504347800570109 1103 10-11 0.5594915852968754 1103 12-13 0.4549098339161475 1103 14-15 0.6518340749691944 1103 16-17 0.8183951995276502 1103 18-19 0.7654414069688045 1103 20-21 0.636230910837412 1103 22-23 0.6911988026953537 1103 24-25 0.7978210167568918 1103 26-27 0.784498559235395 1103 28-29 0.7908788437576604 1103 30-31 0.9532629973132671 1103 32-33 0.901412997676104 1103 34-35 0.8241865067534349 1103 36-37 0.812884668829625 1103 38-39 0.7797907132671469 1103 40-41 0.7624054741565658 1103 42-43 0.7181390589481822 1103 44-45 0.7084246654219228 1103 46-47 0.8521921833422539 1103 48-49 0.8759876371151947 1103 50-51 0.9750370069142136 1103 52-53 0.9887015955601015 1103 54-55 0.9072318770643548 1103 56-57 0.7926676045241265 1103 58-59 0.8739426024856911 1103 60-61 0.9523305900566541 1103 62-63 1.009191760979327 1103 64-65 0.9177278402034261 1103 66-67 1.0856686473049173 1103 68-69 1.1134327029654862 1103 70-71 1.168438992613762 1103 72-73 1.1602962744314809 1103 74-75 1.0785907137998834 1103 76-77 1.0576265597294068 1103 78-79 1.1262227278891572 1103 80-81 1.1516430552992993 1103 82-83 1.3868417683375718 1103 84-85 1.349861406977233 1103 86-87 1.3426939219861467 1103 88-89 1.275881191828038 1103 90-91 1.5254937260291257 1103 92-93 1.550329818905901 1103 94-95 1.408503146818429 1103 96-97 1.2458710217450317 1103 98-99 1.3078528353552628 1103 100-101 1.1544285308228126 1104 1 0.482440050438953 1104 2 0.4561904042312612 1104 3 0.4665509010644193 1104 4 0.38500860047014385 1104 5 0.38759196499666615 1104 6 0.5019736172987734 1104 7 0.5089789087366725 1104 8 0.3636225238661339 1104 9 0.4490140832143439 1104 10-11 0.49035620744800923 1104 12-13 0.4603316133733486 1104 14-15 0.5537684895721569 1104 16-17 0.6163643961233092 1104 18-19 0.5348266168855318 1104 20-21 0.6039099550813987 1104 22-23 0.635195220971589 1104 24-25 0.6271925394016833 1104 26-27 0.7099507746450442 1104 28-29 0.6680261536432539 1104 30-31 0.4895369606546538 1104 32-33 0.49994416366472905 1104 34-35 0.6381905039836724 1104 36-37 0.5427208503293102 1104 38-39 0.6942431405726808 1104 40-41 0.6282638398904936 1104 42-43 0.649845309544979 1104 44-45 0.7680060871872953 1104 46-47 0.80116680824203 1104 48-49 0.8061113486774758 1104 50-51 0.561593467014518 1104 52-53 0.7339835465266091 1104 54-55 0.966246896815413 1104 56-57 0.9883937807895933 1104 58-59 0.8892230289618581 1104 60-61 0.8859454569858443 1104 62-63 1.0096161741178662 1104 64-65 1.046070922196762 1104 66-67 1.0607814979092147 1104 68-69 0.9940310364241363 1104 70-71 0.9903947293682513 1104 72-73 1.0564508821931646 1104 74-75 0.9554429125975652 1104 76-77 1.0818314168467573 1104 78-79 1.0882340236168773 1104 80-81 0.9056448424328067 1104 82-83 0.9283978524935783 1104 84-85 0.9773508468782381 1104 86-87 1.0438479363151139 1104 88-89 1.000422617470921 1104 90-91 0.9123444894031607 1104 92-93 0.8292109879911393 1104 94-95 0.5775399713381972 1104 96-97 0.6496935466282956 1104 98-99 0.45983690981667635 1104 100-101 0.44769945947562917 1105 1 0.5076825269626895 1105 2 0.49426879390194856 1105 3 0.482601340658821 1105 4 0.40629854783561825 1105 5 0.40132794554890694 1105 6 0.40054632862164397 1105 7 0.3609506715187365 1105 8 0.34746199708287406 1105 9 0.5690100924320731 1105 10-11 0.5053376040757982 1105 12-13 0.4624456086087463 1105 14-15 0.5314694583102124 1105 16-17 0.4923902096710506 1105 18-19 0.5227843827288154 1105 20-21 0.5597346234692893 1105 22-23 0.6215825653249993 1105 24-25 0.6326041861590497 1105 26-27 0.7538104451135084 1105 28-29 0.6793606079646111 1105 30-31 0.6027488833600145 1105 32-33 0.5629949707031017 1105 34-35 0.6635500272311887 1105 36-37 0.7617071916336684 1105 38-39 0.6792216414499208 1105 40-41 0.7341712683423438 1105 42-43 0.7540128542648574 1105 44-45 0.7404555271394031 1105 46-47 0.6400476858247828 1105 48-49 0.6367185072648383 1105 50-51 0.43662810651790807 1105 52-53 0.5032367105599249 1105 54-55 0.5319213998535162 1105 56-57 0.6009746245480372 1105 58-59 0.7835900884610183 1105 60-61 0.7252363631124226 1105 62-63 0.7386666356129012 1105 64-65 0.8037317884398618 1105 66-67 0.7910639917587865 1105 68-69 0.721483335625738 1105 70-71 0.7496634915200637 1105 72-73 0.6143611723279037 1105 74-75 0.42127159610176435 1105 76-77 0.5330481430912606 1105 78-79 0.6995745127797122 1105 80-81 0.6625852751406747 1105 82-83 0.7312677116238326 1105 84-85 0.5604349428808888 1105 86-87 0.7205460020024148 1105 88-89 0.5995356417781927 1105 90-91 0.5506432481238726 1105 92-93 0.7109792938797383 1105 94-95 0.6139625757129714 1105 96-97 0.7970413068017486 1105 98-99 0.7671877360789416 1105 100-101 0.5087548753808449 1106 1 0.31356684928207557 1106 2 0.3187907395985903 1106 3 0.13531528139143134 1106 4 0.3883404225910638 1106 5 0.3914286848284263 1106 6 -0.008151747584577151 1106 7 0.19844013105516467 1106 8 0.2529595231706665 1106 9 0.3100909269635821 1106 10-11 0.12025965905522895 1106 12-13 0.138182653852283 1106 14-15 0.38158145001213484 1106 16-17 0.13516613133936772 1106 18-19 -0.135082195029824 1106 20-21 0.1570942865449112 1106 22-23 0.2526998660401958 1106 24-25 0.2927662117803109 1106 26-27 0.05449207333439432 1106 28-29 0.11956148454220994 1106 30-31 0.11669262587866314 1106 32-33 0.19496032435562682 1106 34-35 0.21215187901872667 1106 36-37 0.23470443409558328 1106 38-39 0.27051962921117934 1106 40-41 0.42853130418338736 1106 42-43 0.058707985392622675 1106 44-45 0.1998864231424804 1106 46-47 0.15332836893480817 1106 48-49 0.3954880571910948 1106 50-51 0.1508185225102423 1106 52-53 0.3053476279565004 1106 54-55 0.17531583430352526 1106 56-57 -0.29899009007802846 1106 58-59 -0.7463891894853916 1106 60-61 0.09803425710349245 1106 62-63 0.20182745495595356 1106 64-65 0.3607735590062049 1106 66-67 -0.3130299451304026 1106 68-69 -0.36340192902614277 1106 70-71 -0.15104735595404506 1106 72-73 0.302713387040793 1106 74-75 0.35186220935878865 1106 76-77 0.28348062045328604 1106 78-79 0.4641783742591521 1106 80-81 0.3426358415820623 1106 82-83 0.28527873714552854 1106 84-85 0.3017983382414613 1106 86-87 0.5182356898287317 1106 88-89 0.26082453035837005 1106 90-91 0.41152589184115484 1106 92-93 0.311767796960261 1106 94-95 -0.0689426793535155 1106 96-97 0.08177107501573744 1106 98-99 0.0719630696576985 1106 100-101 -0.2947496753673491 1107 1 0.43283625649039337 1107 2 0.32348401524073367 1107 3 0.3151464281894505 1107 4 0.31423077991079396 1107 5 0.33908308896515393 1107 6 0.292050392229001 1107 7 0.3301182049707734 1107 8 0.3759549364549102 1107 9 0.4441665545373965 1107 10-11 0.3816855581684919 1107 12-13 0.3601342005379493 1107 14-15 0.4221087051765693 1107 16-17 0.5691195346362292 1107 18-19 0.4303810366434533 1107 20-21 0.48923046289950634 1107 22-23 0.5923798168808077 1107 24-25 0.4931010304726442 1107 26-27 0.38079376757529104 1107 28-29 0.49116111216925873 1107 30-31 0.5617663894862801 1107 32-33 0.5382202320609295 1107 34-35 0.6154109566601704 1107 36-37 0.6584462569158234 1107 38-39 0.6646009849261105 1107 40-41 0.6115942713569851 1107 42-43 0.598275807564228 1107 44-45 0.7758986895250288 1107 46-47 0.7194505294684532 1107 48-49 0.6079990283938272 1107 50-51 0.510709784144268 1107 52-53 0.48818294048430033 1107 54-55 0.5891109878513348 1107 56-57 0.6005256884903574 1107 58-59 0.5860310735993224 1107 60-61 0.6086891512230679 1107 62-63 0.6550768660308961 1107 64-65 0.6191987947688595 1107 66-67 0.6352028331458754 1107 68-69 0.5939204863406147 1107 70-71 0.6128057794808548 1107 72-73 0.5714061100075014 1107 74-75 0.6506664183079245 1107 76-77 0.7095682054998065 1107 78-79 0.6043863175150044 1107 80-81 0.669801704102472 1107 82-83 0.6192394779267438 1107 84-85 0.45015776254839324 1107 86-87 0.5549829147185967 1107 88-89 0.7489219136608476 1107 90-91 0.7225331340607966 1107 92-93 0.7739797963214272 1107 94-95 0.7607345641479988 1107 96-97 0.767084014345425 1107 98-99 0.6325767757982774 1107 100-101 0.4250864780816883 1108 1 0.31836841313216624 1108 2 0.3240537768072578 1108 3 0.2667643129488084 1108 4 0.3123419359943753 1108 5 0.2812428655842325 1108 6 0.24143420319646225 1108 7 0.3062021077349968 1108 8 0.22915651366603385 1108 9 0.20825748888022844 1108 10-11 0.21286219681995533 1108 12-13 0.2921133333099206 1108 14-15 0.4493177134276962 1108 16-17 0.3665947600938253 1108 18-19 0.3479759219504146 1108 20-21 0.39470642378932297 1108 22-23 0.49591534778085844 1108 24-25 0.35238265471968333 1108 26-27 0.5196241261117294 1108 28-29 0.31922935922158047 1108 30-31 0.4426171099717706 1108 32-33 0.40392333039985573 1108 34-35 0.4459376438554514 1108 36-37 0.3740796809877409 1108 38-39 0.49184600422459823 1108 40-41 0.5045320056437461 1108 42-43 0.5228849107866367 1108 44-45 0.6153497674094197 1108 46-47 0.5166767529181584 1108 48-49 0.5844030293567712 1108 50-51 0.6173765566285212 1108 52-53 0.479812438723755 1108 54-55 0.5348619488072828 1108 56-57 0.5245225377717233 1108 58-59 0.5238516678179792 1108 60-61 0.6296071386254809 1108 62-63 0.7192709394524286 1108 64-65 0.5715514503934642 1108 66-67 0.675652101549538 1108 68-69 0.5518022761921806 1108 70-71 0.5771174757632167 1108 72-73 0.4927638025492165 1108 74-75 0.5374348219702689 1108 76-77 0.5253313553189933 1108 78-79 0.5438037652477341 1108 80-81 0.5690532761549889 1108 82-83 0.720003447832223 1108 84-85 0.8616259749454542 1108 86-87 0.9186779590164846 1108 88-89 0.8771829140603096 1108 90-91 0.8540852267358368 1108 92-93 0.853689042662122 1108 94-95 0.7344863465129698 1108 96-97 0.8689736188257413 1108 98-99 0.6327793172721563 1108 100-101 0.4471787100126754 1109 1 0.5413136457997645 1109 2 0.5089707221100355 1109 3 0.46225216070265773 1109 4 0.44620171372472583 1109 5 0.43909086687920507 1109 6 0.3410354004295897 1109 7 0.4091703942543674 1109 8 0.44885399887846944 1109 9 0.42035782262494337 1109 10-11 0.40469233344186506 1109 12-13 0.43682703148385826 1109 14-15 0.48589920662855945 1109 16-17 0.5373738225361464 1109 18-19 0.5972982768663186 1109 20-21 0.3781305393967642 1109 22-23 0.5357744883340914 1109 24-25 0.5352327696280881 1109 26-27 0.26847298006929066 1109 28-29 0.26497625349863085 1109 30-31 0.4039299938374725 1109 32-33 0.38885764857393923 1109 34-35 0.38794434452551485 1109 36-37 0.5442586834240686 1109 38-39 0.37677161018724803 1109 40-41 0.5036622021685488 1109 42-43 0.488140328080938 1109 44-45 0.46665268120595016 1109 46-47 0.577008817052409 1109 48-49 0.5738549384652103 1109 50-51 0.5950136244590141 1109 52-53 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-0.7742249531383365 2316 98-99 -0.7146210360778689 2316 100-101 -0.5983984034789955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10260.0 3 3724.0 4 3791.0 5 4196.0 6 5224.0 7 6445.0 8 8287.0 9 10558.0 10 13267.0 11 16509.0 12 19939.0 13 23525.0 14 26588.0 15 31175.0 16 35892.0 17 41048.0 18 39229.0 19 36984.0 20 39009.0 21 42022.0 22 46473.0 23 51943.0 24 58055.0 25 64911.0 26 73251.0 27 84702.0 28 96768.0 29 111643.0 30 128872.0 31 151654.0 32 180869.0 33 218119.0 34 270713.0 35 338439.0 36 407094.0 37 406772.0 38 198570.0 39 12251.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.243268380019 17.968585622487122 14.345393935497075 23.442752061996803 2 15.898210407161386 19.45391267124578 39.74551094510941 24.902365976483427 3 19.60571525379993 23.312346373464187 29.2103392580347 27.87159911470118 4 13.198769888248671 15.967968377168349 35.863316628968036 34.96994510561494 5 14.306094798326384 36.99741384560375 33.55265498384935 15.143836372220518 6 33.48608556697923 35.14056140139295 16.861579280610243 14.511773751017579 7 29.841106497289584 32.13185080155102 19.653915157127148 18.373127544032254 8 27.52149422058357 34.06455681093405 19.174526681046384 19.239422287435996 9 26.974465173342015 16.526347202503143 19.342653801285937 37.15653382286891 10-11 25.739129176594744 26.00103605217398 26.658538816763304 21.601295954467965 12-13 26.698736841248564 23.18969626827779 26.96405579294029 23.147511097533354 14-15 23.221887347742882 24.72730034804641 25.192793959358717 26.858018344851992 16-17 23.03355487440338 27.836983830430583 26.06297742336699 23.066483871799047 18-19 23.125774870225722 26.13157051518753 28.24554761414575 22.497107000440998 20-21 23.586635220291686 25.455239636420256 28.413911026574816 22.544214116713242 22-23 23.253286298503163 25.402395592986736 28.4341012969145 22.910216811595603 24-25 23.026752487293116 25.651754089080487 28.55634861827937 22.765144805347028 26-27 22.545355279885467 25.96021692514129 28.2306866050323 23.26374118994094 28-29 22.712124466349394 26.465573177486633 28.082686170112552 22.73961618605142 30-31 22.759820648784 25.940492006944687 27.99521384403752 23.30447350023379 32-33 22.38727185468156 25.937964249194494 28.062492162026587 23.612271734097355 34-35 23.279915507398 26.094340928010812 27.776370137164825 22.84937342742636 36-37 23.542400763033168 25.489142181547752 27.80766331040998 23.1607937450091 38-39 22.859267664723447 25.917634320615377 27.806286868613807 23.416811146047365 40-41 23.348344366070688 25.74315763332413 27.993305613857007 22.915192386748178 42-43 23.087852612414572 25.825330907654166 27.63407224326751 23.45274423666375 44-45 22.992405009891115 26.043192457139757 27.96343978774406 23.00096274522507 46-47 23.537650226399045 26.361404022129562 27.153525688015474 22.94742006345592 48-49 23.153852041769575 26.46792305680214 27.017345126983038 23.360879774445245 50-51 23.27630564094868 26.574943825862206 27.078397896865592 23.070352636323516 52-53 23.71681906612441 26.129360139244813 27.093162154148054 23.06065864048273 54-55 23.0582975559909 26.452768031735385 27.22261748815182 23.2663169241219 56-57 23.1052166245895 26.697842342307048 26.93795039092893 23.25899064217452 58-59 23.107062236530094 26.08430795916049 27.144967850983576 23.663661953325843 60-61 22.644931985452825 26.403922078552167 27.525411378734827 23.425734557260185 62-63 22.859940909440176 26.368537064184522 27.61142758995792 23.160094436417385 64-65 22.603591930210484 26.40758765123322 27.3273869611728 23.661433457383495 66-67 22.565119505726503 26.554981643883046 27.238588238555543 23.64131061183491 68-69 23.231925402688134 26.054998762999432 27.41884839314982 23.29422744116262 70-71 22.970647855640685 26.12981886370746 27.051835202199843 23.84769807845201 72-73 22.974529520170094 26.313840070762218 27.09109429788542 23.62053611118227 74-75 23.00923427723446 26.729782604731916 26.859358737528545 23.401624380505083 76-77 23.193188982120233 26.32201237468313 26.977677113104313 23.507121530092324 78-79 23.224394569107677 26.64878265959442 26.808593664662062 23.31822910663584 80-81 23.475192339767332 26.56413969811738 26.596427978978117 23.364239983137168 82-83 23.35576209293037 26.705627087959115 26.682424403318105 23.25618641579241 84-85 23.22149750218428 26.830278857090168 26.659001786747694 23.28922185397786 86-87 23.537990152871856 26.651973418358903 26.388046397637993 23.421990031131244 88-89 23.32382513064988 26.823123016591833 26.186337665171617 23.66671418758667 90-91 23.209756049039466 26.863074918160297 26.36321259242525 23.563956440374987 92-93 23.487353360005425 26.43837164621053 26.53014172374042 23.544133270043623 94-95 23.269849757110293 26.19022286415447 26.795766783683334 23.744160595051905 96-97 23.713837936263047 25.769689794120932 26.696700964522645 23.819771305093376 98-99 23.2756729290746 26.16108406422218 26.298511861887476 24.264731144815745 100-101 23.99026241374062 25.49336163482121 26.60261926529462 23.913756686143547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 381.0 1 274.5 2 259.0 3 357.0 4 317.0 5 304.0 6 560.5 7 1252.5 8 2041.5 9 2338.0 10 2146.5 11 1890.0 12 1892.5 13 2103.5 14 2295.5 15 2524.0 16 2771.5 17 2987.0 18 3354.0 19 3822.0 20 4320.5 21 5038.0 22 6045.0 23 7494.5 24 9233.0 25 11304.5 26 14722.5 27 19129.0 28 22668.0 29 26372.0 30 30958.5 31 38484.0 32 50018.0 33 54819.0 34 55102.0 35 59128.5 36 65346.0 37 72219.5 38 77688.0 39 81937.5 40 87402.0 41 94059.5 42 97828.0 43 100475.0 44 104594.0 45 111611.5 46 115860.5 47 118705.5 48 121905.5 49 121246.5 50 119708.0 51 119156.5 52 120411.0 53 124804.0 54 129504.0 55 124035.0 56 113816.5 57 105253.5 58 99305.0 59 89729.0 60 73913.0 61 62763.5 62 54055.0 63 41572.5 64 29302.5 65 21735.5 66 16436.0 67 12774.5 68 9931.0 69 7769.5 70 6240.0 71 4478.0 72 3269.5 73 2668.5 74 2043.5 75 1428.0 76 1031.0 77 775.5 78 551.0 79 337.5 80 196.0 81 122.0 82 73.0 83 42.5 84 36.0 85 33.0 86 19.5 87 12.5 88 12.0 89 10.5 90 5.5 91 2.0 92 3.0 93 1.5 94 2.5 95 3.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18332087390181487 2 0.04628219301663176 3 0.04438389994368397 4 0.05044035879546977 5 0.05655708091941264 6 0.06240261831864868 7 0.036248358202479174 8 0.0341994069491387 9 0.04040652398131718 10-11 0.041295407245634 12-13 0.03780013746052379 14-15 0.033446116047175296 16-17 0.030930124434617512 18-19 0.038568494180526464 20-21 0.047894235546833454 22-23 0.04558916538682543 24-25 0.06518979465591329 26-27 0.05068141188409806 28-29 0.042440409416618384 30-31 0.05134430787782586 32-33 0.04781890645663711 34-35 0.046930023192320286 36-37 0.04471534794054787 38-39 0.024813402310674644 40-41 0.023110964872237343 42-43 0.0199622089020303 44-45 0.004097902506680936 46-47 0.012188246793767933 48-49 0.011299363529451113 50-51 0.008903898461207477 52-53 0.009009359187482354 54-55 0.009943439905916979 56-57 0.00798488356081212 58-59 0.009581860272974543 60-61 0.009837979179642102 62-63 0.004097902506680936 64-65 0.006659091573356523 66-67 0.003691125419620697 68-69 0.007834225380419437 70-71 0.02160438306831053 72-73 0.01856108782437836 74-75 0.019419839452616647 76-77 0.011540416618079403 78-79 0.004926522498840686 80-81 0.006915210480024081 82-83 0.005709945036882629 84-85 0.005167575587468976 86-87 0.007231592658848712 88-89 0.028142948097352904 90-91 0.03487736876090577 92-93 0.020971618710661267 94-95 0.03043295243932167 96-97 0.03373236658992139 98-99 0.02003753799222664 100-101 0.12011976722708496 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3318771.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.189331852460825 #Duplication Level Percentage of deduplicated Percentage of total 1 72.84677318387757 33.64743780971066 2 13.959096692116466 12.895226989455114 3 5.136052493103863 7.116924990468992 4 2.4069120658701704 4.446946406006774 5 1.4094730235985853 3.25513086120432 6 0.8732612181419038 2.420121131918435 7 0.5960021679707652 1.927025934483144 8 0.4431843474910791 1.6376311118465412 9 0.32890616650198556 1.367276046559285 >10 1.7796869862980316 15.078238967187744 >50 0.1382345364325048 4.363623160674733 >100 0.07402736315633646 6.235650093235277 >500 0.004391512614553064 1.3245822233332296 >1k 0.003867152897546007 3.787042093419837 >5k 1.310899287303731E-4 0.49714218049586406 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9168 0.27624683956802076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7253 0.2185447564776238 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4924 0.14836817605071276 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4913 0.14803672805384885 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4884 0.1471629106075713 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4457 0.1342967020020363 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4356 0.13125340675810412 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4028 0.12137023012434422 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3992 0.12028549122551692 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3592 0.10823283679410241 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3573 0.1076603357086102 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3489 0.10512927827801316 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0131636078536302E-5 2 0.0 0.0 0.0 0.0 3.0131636078536302E-5 3 0.0 0.0 0.0 0.0 3.0131636078536302E-5 4 0.0 0.0 0.0 0.0 3.0131636078536302E-5 5 0.0 0.0 0.0 0.0 3.0131636078536302E-5 6 0.0 0.0 0.0 0.0 3.0131636078536302E-5 7 0.0 0.0 0.0 0.0 3.0131636078536302E-5 8 0.0 0.0 0.0 0.0 3.0131636078536302E-5 9 0.0 0.0 0.0 0.0 3.0131636078536302E-5 10-11 0.0 0.0 0.0 1.5065818039268151E-5 3.0131636078536302E-5 12-13 0.0 0.0 0.0 3.0131636078536302E-5 2.561189066675586E-4 14-15 0.0 0.0 0.0 4.519745411780445E-5 3.3144799686389933E-4 16-17 0.0 0.0 0.0 1.506581803926815E-4 3.3144799686389933E-4 18-19 0.0 0.0 0.0 1.506581803926815E-4 3.3144799686389933E-4 20-21 0.0 0.0 0.0 2.4105308862829041E-4 3.615796329424356E-4 22-23 0.0 0.0 0.0 4.218429050995082E-4 3.615796329424356E-4 24-25 0.0 1.5065818039268151E-5 0.0 8.286199921597483E-4 3.615796329424356E-4 26-27 0.0 4.519745411780445E-5 0.0 0.0018229639827514463 3.766454509817038E-4 28-29 0.0 6.0263272157072603E-5 0.0 0.004685469410212395 3.9171126902097194E-4 30-31 0.0 6.0263272157072603E-5 0.0 0.014222132229069134 3.9171126902097194E-4 32-33 0.0 6.0263272157072603E-5 0.0 0.03418434113109943 3.9171126902097194E-4 34-35 0.0 7.532909019634075E-5 0.0 0.06401466084885037 3.9171126902097194E-4 36-37 0.0 9.03949082356089E-5 0.0 0.11374692619647453 4.218429050995082E-4 38-39 0.0 9.03949082356089E-5 0.0 0.18765982949712406 4.3690872313877636E-4 40-41 0.0 9.03949082356089E-5 0.0 0.2684879432777977 4.5197454117804455E-4 42-43 0.0 9.03949082356089E-5 0.0 0.3543028428294691 4.5197454117804455E-4 44-45 0.0 9.03949082356089E-5 0.0 0.4593266603812074 5.273036313743853E-4 46-47 0.0 9.03949082356089E-5 0.0 0.5843729501071331 6.02632721570726E-4 48-49 0.0 9.03949082356089E-5 0.0 0.7168316223083786 6.327643576492624E-4 50-51 0.0 9.03949082356089E-5 0.0 0.8648532845441882 6.327643576492624E-4 52-53 0.0 9.03949082356089E-5 0.0 1.0033533497791804 6.628959937277987E-4 54-55 0.0 9.03949082356089E-5 0.0 1.1531377127255844 6.628959937277987E-4 56-57 0.0 1.2052654431414521E-4 0.0 1.3369858902587737 6.628959937277987E-4 58-59 0.0 1.2052654431414521E-4 0.0 1.5307172444257227 6.628959937277987E-4 60-61 0.0 1.2052654431414521E-4 0.0 1.744064293679799 6.779618117670668E-4 62-63 0.0 1.2052654431414521E-4 0.0 1.948356786292275 6.930276298063349E-4 64-65 0.0 1.2052654431414521E-4 0.0 2.1636171944373386 6.930276298063349E-4 66-67 0.0 1.2052654431414521E-4 0.0 2.4186513622060692 6.930276298063349E-4 68-69 0.0 1.2052654431414521E-4 0.0 2.695380307951347 6.930276298063349E-4 70-71 0.0 1.2052654431414521E-4 0.0 2.9884707320872694 7.080934478456031E-4 72-73 0.0 1.2052654431414521E-4 0.0 3.2910827532240097 7.382250839241394E-4 74-75 0.0 1.2052654431414521E-4 0.0 3.5934537212721214 7.834225380419439E-4 76-77 0.0 1.2052654431414521E-4 0.0 3.924464809412882 8.135541741204802E-4 78-79 0.0 1.2052654431414521E-4 0.0 4.298413478965557 8.436858101990164E-4 80-81 0.0 1.2052654431414521E-4 0.0 4.688316849821817 8.738174462775527E-4 82-83 0.0 1.2052654431414521E-4 0.0 5.098965852118148 9.340807184346254E-4 84-85 0.0 1.2052654431414521E-4 0.0 5.508424654789378 9.642123545131617E-4 86-87 0.0 1.2052654431414521E-4 0.0 5.93700198055244 9.7927817255243E-4 88-89 0.0 1.2052654431414521E-4 0.0 6.386204411211258 9.94343990591698E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6220 0.0 51.143177 1 GTATCAA 10920 0.0 40.513027 1 ATCAACG 13775 0.0 31.949354 3 TATCAAC 13810 0.0 31.799553 2 TCAACGC 13965 0.0 31.548704 4 CAACGCA 14220 0.0 30.846941 5 AACGCAG 14675 0.0 30.015535 6 ACGCAGA 16970 0.0 25.728392 7 CGCAGAG 16950 0.0 25.646633 8 TAAGGTG 1970 0.0 25.329874 5 TACATGG 13730 0.0 24.127092 2 GTACATG 14340 0.0 23.711044 1 ACATGGG 13800 0.0 22.971506 3 GAGTACT 9960 0.0 22.605265 12-13 GCAGAGT 19130 0.0 22.052809 9 GTAAGGT 2105 0.0 21.675135 4 GTACTTT 10550 0.0 21.118513 14-15 GTGTAAG 1350 0.0 20.81229 1 AGGTAAG 1940 0.0 20.578812 2 CAGAGTA 17805 0.0 20.477152 10-11 >>END_MODULE