FastQCFastQC Report
Thu 26 May 2016
SRR938422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3318771
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87300.2630491829656219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76060.22918122401334712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50660.1526468683738649No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48500.14613843498090107No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA47360.14270342846794792No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47250.14237198047108401No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC45150.1360443368945914No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA44210.13321196310320899No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG41510.1250764213620042No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35980.1084136266105736No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA35200.10606335899644777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58750.050.842721
GTATCAA102750.040.180461
ATCAACG134250.030.3804743
TATCAAC137100.029.9043052
TCAACGC136750.029.8598334
CAACGCA139350.029.2685955
AACGCAG144150.028.2696916
AGGTAAG19500.024.8788052
TAAGGTG21750.024.6968045
ACGCAGA167100.024.3302047
TACATGG136000.024.270822
CGCAGAG168800.024.0288988
GTACATG142900.023.8984221
ACATGGG135800.023.0678543
GGTAAGG22400.022.9190863
GTAAGGT22850.022.4677264
AAGGTAA21150.021.5892681
TAAGGTA10650.020.9782754
GCAGAGT190000.020.947829
CATGGGG77550.020.9023024