##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938422_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3318771 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.838992506563425 31.0 31.0 34.0 30.0 34.0 2 32.065511600529234 33.0 31.0 34.0 30.0 34.0 3 32.1334463872319 33.0 31.0 34.0 30.0 34.0 4 35.79136764784313 37.0 35.0 37.0 35.0 37.0 5 35.657058893186665 37.0 35.0 37.0 33.0 37.0 6 35.686182626038374 37.0 35.0 37.0 33.0 37.0 7 35.62674255017897 37.0 35.0 37.0 33.0 37.0 8 35.612338422867985 37.0 35.0 37.0 33.0 37.0 9 37.207226108701086 39.0 37.0 39.0 33.0 39.0 10-11 37.1204239460933 39.0 37.0 39.0 33.0 39.0 12-13 37.018134725173866 39.0 37.0 39.0 33.0 39.0 14-15 38.093961891314585 40.0 37.0 41.0 33.0 41.0 16-17 38.01077989412346 40.0 37.0 41.0 33.0 41.0 18-19 38.113762895963596 40.0 37.5 41.0 33.0 41.0 20-21 38.16307181182432 40.0 37.0 41.0 33.0 41.0 22-23 38.07549948459837 40.0 37.0 41.0 33.0 41.0 24-25 37.970815702559776 40.0 37.0 41.0 33.0 41.0 26-27 37.78452460263152 40.0 37.0 41.0 32.0 41.0 28-29 37.66693860468227 40.0 37.0 41.0 32.0 41.0 30-31 37.43133000137702 39.0 36.5 41.0 31.5 41.0 32-33 37.220951520909395 39.0 36.0 41.0 31.0 41.0 34-35 37.0724718578052 39.0 36.0 41.0 31.0 41.0 36-37 37.06660658418433 39.0 36.0 41.0 31.0 41.0 38-39 36.93940738906059 39.0 36.0 41.0 31.0 41.0 40-41 36.762504101668966 39.0 35.0 41.0 30.0 41.0 42-43 36.544025634790714 39.0 35.0 41.0 30.0 41.0 44-45 36.27619546512851 39.0 35.0 40.0 30.0 41.0 46-47 35.91244740899568 38.5 35.0 40.0 28.5 41.0 48-49 35.545439712471875 38.0 34.5 40.0 27.5 41.0 50-51 35.18127086804121 38.0 34.0 40.0 26.5 41.0 52-53 34.69183938873758 38.0 33.5 40.0 26.0 41.0 54-55 34.37389277536775 38.0 33.0 40.0 25.0 41.0 56-57 34.03145215502968 37.0 33.0 40.0 24.0 41.0 58-59 33.58119315855176 36.5 32.5 39.5 23.0 41.0 60-61 33.20839988658452 36.0 32.0 39.0 22.5 40.5 62-63 32.753426343667584 35.5 31.0 39.0 21.5 40.0 64-65 32.25551552065508 35.0 31.0 38.5 20.0 40.0 66-67 31.764748004607732 35.0 30.5 38.0 18.5 40.0 68-69 31.234632338296315 34.5 30.0 37.5 17.5 39.5 70-71 30.66293139840019 34.0 29.0 37.0 13.5 39.0 72-73 30.233431743256766 34.0 29.0 36.0 11.0 39.0 74-75 29.737865312189363 34.0 29.0 36.0 7.5 38.5 76-77 28.46857737397368 32.5 27.0 34.5 7.0 36.5 78-79 29.17176298093481 33.5 29.0 35.0 6.5 37.0 80-81 28.923924247861635 33.5 28.5 35.0 2.0 37.0 82-83 28.48002212264721 33.0 27.5 35.0 2.0 36.0 84-85 27.986814998684757 33.0 26.5 35.0 2.0 36.0 86-87 27.449407777758694 33.0 26.0 35.0 2.0 35.0 88-89 26.825212706751987 32.0 25.0 34.5 2.0 35.0 90-91 26.31622850748063 32.0 24.5 34.0 2.0 35.0 92-93 25.616460581341705 31.5 23.0 34.0 2.0 35.0 94-95 24.884314404338234 31.0 19.0 34.0 2.0 35.0 96-97 23.93257850571793 31.0 8.5 34.0 2.0 35.0 98-99 22.73608031406807 30.5 2.0 34.0 2.0 35.0 100-101 19.051048716527895 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2865661805782871 1101 2 0.3820481122236359 1101 3 0.3454622163698886 1101 4 0.2212090298505771 1101 5 0.08797040687644397 1101 6 0.17670959904217654 1101 7 0.28570843463276674 1101 8 0.2198069250732715 1101 9 0.3812575523313413 1101 10-11 0.33437093770353243 1101 12-13 0.391535628485542 1101 14-15 0.4640256587173468 1101 16-17 0.4838216745581789 1101 18-19 0.46006653939601705 1101 20-21 0.44115050529073585 1101 22-23 0.45873258498041025 1101 24-25 0.4217377191511815 1101 26-27 0.48792264332350754 1101 28-29 0.3307868674934369 1101 30-31 0.3948426259379403 1101 32-33 0.3863812858340836 1101 34-35 0.17544111633447557 1101 36-37 0.4930174688798985 1101 38-39 0.5771768648452849 1101 40-41 0.6318328313959043 1101 42-43 0.7173876678209083 1101 44-45 0.6996188698421477 1101 46-47 0.7068181352941423 1101 48-49 0.9503708172497909 1101 50-51 0.8224751419882637 1101 52-53 0.8004754483637626 1101 54-55 0.887471828132 1101 56-57 0.8588191273293404 1101 58-59 0.9330826435435995 1101 60-61 0.7732510136233444 1101 62-63 0.8248463284958163 1101 64-65 0.8161254663722843 1101 66-67 0.7544128322727737 1101 68-69 0.6462599465066354 1101 70-71 0.6446706333068093 1101 72-73 0.4567587615343811 1101 74-75 0.49764233702113003 1101 76-77 0.5972394097998119 1101 78-79 0.5770703892028273 1101 80-81 0.6087667861454094 1101 82-83 0.5894203627446188 1101 84-85 0.673789241981332 1101 86-87 0.5869156946104468 1101 88-89 0.549609236559359 1101 90-91 0.6936775992452588 1101 92-93 0.6646888397523547 1101 94-95 0.7610193227281812 1101 96-97 0.8235765976222496 1101 98-99 0.7266805559648937 1101 100-101 0.7562276957567953 1102 1 0.29918925456716394 1102 2 0.3122056608001458 1102 3 0.3320191500099199 1102 4 0.2439918486177035 1102 5 0.1496034562447477 1102 6 0.20912391827743448 1102 7 0.22435060401770812 1102 8 0.347074618945868 1102 9 0.36758850837263424 1102 10-11 0.3505494889039298 1102 12-13 0.36886967076820554 1102 14-15 0.4811492701977045 1102 16-17 0.44317576007133397 1102 18-19 0.48086907774285237 1102 20-21 0.4633890127982738 1102 22-23 0.47528535739611755 1102 24-25 0.5514359147980841 1102 26-27 0.45531263095542585 1102 28-29 0.43009217421033696 1102 30-31 0.4075922705000252 1102 32-33 0.34535294327285015 1102 34-35 0.309303599429505 1102 36-37 0.4229482885254967 1102 38-39 0.40485923628079235 1102 40-41 0.4891366470074203 1102 42-43 0.44738772561568396 1102 44-45 0.4928946203073892 1102 46-47 0.46435156957569745 1102 48-49 0.5371678882102273 1102 50-51 0.5420933496564828 1102 52-53 0.3904386908822204 1102 54-55 0.38296620469969156 1102 56-57 0.3614755489075989 1102 58-59 0.38915288732199826 1102 60-61 0.482063330847339 1102 62-63 0.5612690626513839 1102 64-65 0.47461921875631674 1102 66-67 0.6046123398612302 1102 68-69 0.4490596413501855 1102 70-71 0.335973501083906 1102 72-73 0.2674867446120679 1102 74-75 0.2030704807683108 1102 76-77 0.2789690342493145 1102 78-79 0.417690411627202 1102 80-81 0.5892137709916199 1102 82-83 0.28488116054379375 1102 84-85 0.11636787178263575 1102 86-87 0.025171621465368332 1102 88-89 0.1837368243207358 1102 90-91 -0.004449560834729027 1102 92-93 0.15235462408534417 1102 94-95 0.13452775498691238 1102 96-97 0.07395000971060384 1102 98-99 -0.038548080193830714 1102 100-101 -0.14830335737972788 1103 1 0.2888506629901819 1103 2 0.30046858390311115 1103 3 0.27765393413799444 1103 4 0.19258708398280078 1103 5 0.1872251793979487 1103 6 0.17781296114603862 1103 7 0.22523243823287942 1103 8 0.20127209520732947 1103 9 0.3051753949791305 1103 10-11 0.2982785064368727 1103 12-13 0.3534787970697977 1103 14-15 0.4678164828917488 1103 16-17 0.3997286346324671 1103 18-19 0.36608413062560174 1103 20-21 0.387329091753827 1103 22-23 0.41307667665286374 1103 24-25 0.39326101130841806 1103 26-27 0.4486064865093411 1103 28-29 0.4391444890934082 1103 30-31 0.5737562097599422 1103 32-33 0.5061936070237323 1103 34-35 0.4157500532547047 1103 36-37 0.4150084498219897 1103 38-39 0.4709456396530598 1103 40-41 0.46116038827781125 1103 42-43 0.6116882372433352 1103 44-45 0.5517166208425053 1103 46-47 0.5192085923068177 1103 48-49 0.6554570752300606 1103 50-51 0.669729651740667 1103 52-53 0.6752483184369495 1103 54-55 0.5718558580269075 1103 56-57 0.6857225498522297 1103 58-59 0.7563131081354726 1103 60-61 0.5682517867365249 1103 62-63 0.6872172118915856 1103 64-65 0.6183628916640771 1103 66-67 0.5471324746207848 1103 68-69 0.4843984240367476 1103 70-71 0.39895683086806244 1103 72-73 0.5131249687126278 1103 74-75 0.5193892733925729 1103 76-77 0.4527002031301244 1103 78-79 0.47016134932001563 1103 80-81 0.4475968201177025 1103 82-83 0.4255894126555546 1103 84-85 0.27147644932671255 1103 86-87 0.4912703104536362 1103 88-89 0.5437711148400624 1103 90-91 0.5442405194858999 1103 92-93 0.7016990636155427 1103 94-95 0.6651627848273094 1103 96-97 0.6000104767824226 1103 98-99 0.561006088452185 1103 100-101 0.31093427919536865 1104 1 0.30400743175834677 1104 2 0.2720042158613154 1104 3 0.24002149662915429 1104 4 0.21605325717857937 1104 5 0.09846172955088406 1104 6 0.13903725945068857 1104 7 0.13864542618630082 1104 8 0.21544063797063018 1104 9 0.28371508651424904 1104 10-11 0.3363741943040992 1104 12-13 0.245480359672392 1104 14-15 0.3738805367559692 1104 16-17 0.3734774185096583 1104 18-19 0.31881669670118384 1104 20-21 0.3066248828555729 1104 22-23 0.428182387422666 1104 24-25 0.34395705714089075 1104 26-27 0.32863039159490626 1104 28-29 0.3541037680094803 1104 30-31 0.40295244708580213 1104 32-33 0.4787450135214115 1104 34-35 0.4107270543709305 1104 36-37 0.5934776440356373 1104 38-39 0.6719332753321794 1104 40-41 0.5089240583060217 1104 42-43 0.5826110696552504 1104 44-45 0.5345545051730412 1104 46-47 0.5746455131014656 1104 48-49 0.5438134281603411 1104 50-51 0.6093444030902901 1104 52-53 0.7103746678232099 1104 54-55 0.7741109852131061 1104 56-57 0.6683398387740098 1104 58-59 0.6361726541066233 1104 60-61 0.5329968084781171 1104 62-63 0.7018268936894358 1104 64-65 0.7501832511910962 1104 66-67 0.6272886644554596 1104 68-69 0.3521647329245887 1104 70-71 0.2679125425595963 1104 72-73 0.1249616108735232 1104 74-75 0.3094934669216478 1104 76-77 0.2121811970232521 1104 78-79 0.10430862792870599 1104 80-81 0.28449090511590924 1104 82-83 0.22906350035890455 1104 84-85 0.29634076243714347 1104 86-87 0.34097203024857947 1104 88-89 0.4319489201249489 1104 90-91 0.5170991542840859 1104 92-93 0.5491111998428373 1104 94-95 0.5695275808656248 1104 96-97 0.42040428412040143 1104 98-99 0.2761496318524159 1104 100-101 0.0991763574899629 1105 1 0.25580481682260015 1105 2 0.2414044548215699 1105 3 0.22310388924912417 1105 4 0.19046436128689237 1105 5 0.04326950281605235 1105 6 0.20394334223021815 1105 7 0.16537568580615414 1105 8 0.1493274895597665 1105 9 0.2530898234024619 1105 10-11 0.28251561886736454 1105 12-13 0.2908531206366973 1105 14-15 0.44686483886791706 1105 16-17 0.3102191881295795 1105 18-19 0.34793816491970375 1105 20-21 0.42750601837913393 1105 22-23 0.4100085596541234 1105 24-25 0.41351045453763646 1105 26-27 0.39680984052628077 1105 28-29 0.3927466100304642 1105 30-31 0.4343521652976392 1105 32-33 0.4439737656476055 1105 34-35 0.44856589976948413 1105 36-37 0.5021400262893039 1105 38-39 0.4720694486100001 1105 40-41 0.39465720637621615 1105 42-43 0.4328248928489913 1105 44-45 0.42899700019064113 1105 46-47 0.14929479877577734 1105 48-49 0.07937713832248505 1105 50-51 0.020960499569881108 1105 52-53 0.08904155187394736 1105 54-55 0.016158840532646934 1105 56-57 0.1345732460017075 1105 58-59 0.15766914878841476 1105 60-61 0.08613928183842745 1105 62-63 -0.011832483310520558 1105 64-65 0.049792738545917814 1105 66-67 0.04317927808690314 1105 68-69 0.07158193270947777 1105 70-71 0.04450772393335001 1105 72-73 0.23450323059577372 1105 74-75 0.2000786275384705 1105 76-77 0.16867587881920443 1105 78-79 0.17678079587250295 1105 80-81 0.36133385566671095 1105 82-83 0.2886979544369197 1105 84-85 0.1339392362062668 1105 86-87 0.25296686169886584 1105 88-89 0.19046079635229773 1105 90-91 0.22329051229375807 1105 92-93 0.26047188625738826 1105 94-95 0.13226846959337024 1105 96-97 0.06470887814347037 1105 98-99 -0.10553890705892854 1105 100-101 -0.5622734645065499 1106 1 0.09826006760575723 1106 2 0.08823092440877645 1106 3 0.16300632194875675 1106 4 0.02342627206861181 1106 5 -0.0036353169715468425 1106 6 -0.10113103720794925 1106 7 0.07906701287424767 1106 8 0.04196872945631469 1106 9 -0.02126053442920295 1106 10-11 0.07091131516310156 1106 12-13 0.0701613976500397 1106 14-15 0.11860185632769316 1106 16-17 -0.024322321964028504 1106 18-19 0.25127318843171764 1106 20-21 0.16652983600653215 1106 22-23 0.16792144367776984 1106 24-25 0.12858555172291375 1106 26-27 0.10020584938929034 1106 28-29 0.1922984579977438 1106 30-31 0.2908554866145039 1106 32-33 0.2901740819193961 1106 34-35 0.043499392396377345 1106 36-37 -0.36020645107735305 1106 38-39 -0.023599433811448023 1106 40-41 0.14721440040177214 1106 42-43 0.19683445972334113 1106 44-45 0.12057004716603359 1106 46-47 -0.10713980404381829 1106 48-49 0.03911083099243484 1106 50-51 0.03190868980527739 1106 52-53 0.11928273805523304 1106 54-55 -0.21254557963187892 1106 56-57 0.05911297330411003 1106 58-59 0.1077787325645545 1106 60-61 -0.16746725845844423 1106 62-63 -0.2270094079725382 1106 64-65 -0.44290758814478437 1106 66-67 -0.2009485086820071 1106 68-69 -0.834830883012426 1106 70-71 -0.5830238048448351 1106 72-73 -0.3582778542210967 1106 74-75 -0.16054599767019795 1106 76-77 -0.21009945939017882 1106 78-79 -0.23500419487782054 1106 80-81 -0.8638483710626552 1106 82-83 -0.40971999201914144 1106 84-85 -0.2083415788766061 1106 86-87 -0.021886845233410668 1106 88-89 0.08527940313570426 1106 90-91 0.07256519411183504 1106 92-93 -0.11564551864690742 1106 94-95 -0.06964323825114604 1106 96-97 -0.032878658062237776 1106 98-99 -0.022645923152669667 1106 100-101 -0.26443638383871715 1107 1 0.1859915135770045 1107 2 0.15386139468723314 1107 3 0.10389360417957505 1107 4 0.05748690945959112 1107 5 -0.013141124266439874 1107 6 0.10280024331090942 1107 7 0.10190751592796943 1107 8 0.16326583908288228 1107 9 0.17269456927274263 1107 10-11 0.1832986786491091 1107 12-13 0.18947537263323966 1107 14-15 0.2752359140931375 1107 16-17 0.21010693207824005 1107 18-19 0.24272431868449473 1107 20-21 0.3272032752237948 1107 22-23 0.2918258119151105 1107 24-25 0.31508759255925867 1107 26-27 0.3414319955603915 1107 28-29 0.3094238837994894 1107 30-31 0.27934198424233614 1107 32-33 0.2820136260217012 1107 34-35 0.23836739122798178 1107 36-37 0.2146039738943344 1107 38-39 0.23289327668679505 1107 40-41 0.3206652630727902 1107 42-43 0.2607638682205362 1107 44-45 0.3662212742946238 1107 46-47 0.3135903667111748 1107 48-49 0.2596631334214763 1107 50-51 0.16945119117646357 1107 52-53 0.2384718094414282 1107 54-55 0.16060205580686926 1107 56-57 0.15640446966759214 1107 58-59 0.028931158907766985 1107 60-61 -0.004895836191295189 1107 62-63 0.19688126596226851 1107 64-65 0.27327540871498 1107 66-67 0.25998617299725524 1107 68-69 0.059329451352706286 1107 70-71 0.06456986803715736 1107 72-73 0.08074042714743968 1107 74-75 0.077813188474952 1107 76-77 -0.013292061875045391 1107 78-79 0.11397483532739727 1107 80-81 0.10945772076552274 1107 82-83 0.15419409356919545 1107 84-85 0.08166819976602113 1107 86-87 0.170660345823471 1107 88-89 0.06964018566527486 1107 90-91 0.14630489799268176 1107 92-93 0.06470940895541233 1107 94-95 0.10307405110351198 1107 96-97 0.14201684845534146 1107 98-99 -0.19606937970671012 1107 100-101 -0.40388965194374293 1108 1 0.1504440795212041 1108 2 0.14513441993266696 1108 3 0.19581105852590497 1108 4 0.09817244869278596 1108 5 -0.012949991251090864 1108 6 0.017059559888025433 1108 7 0.09143455099856368 1108 8 0.09454359966530745 1108 9 0.1557544964173374 1108 10-11 0.1728807719326042 1108 12-13 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-0.5267365907181798 2316 64-65 -0.4567791137187811 2316 66-67 -0.3788785062816622 2316 68-69 -0.4513540625929302 2316 70-71 -0.6168412415015219 2316 72-73 -0.45583187096975664 2316 74-75 -0.18603560756011106 2316 76-77 -0.09776340579319509 2316 78-79 -0.1207641149030998 2316 80-81 -0.3322236749124272 2316 82-83 -0.25019054740926805 2316 84-85 -0.31719166937411103 2316 86-87 -0.16311582389086965 2316 88-89 0.03187942947231548 2316 90-91 -0.036792093675767745 2316 92-93 -0.08002932297457477 2316 94-95 -0.01697444171966822 2316 96-97 0.13427365416186632 2316 98-99 0.15848828324332942 2316 100-101 0.4083269714141089 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 14.0 4 42.0 5 121.0 6 252.0 7 648.0 8 1375.0 9 2517.0 10 4134.0 11 6518.0 12 8962.0 13 12670.0 14 16329.0 15 21468.0 16 26339.0 17 32782.0 18 31583.0 19 27586.0 20 27121.0 21 28620.0 22 31201.0 23 34925.0 24 40607.0 25 48322.0 26 57323.0 27 69324.0 28 82479.0 29 98660.0 30 118647.0 31 142005.0 32 171998.0 33 212998.0 34 274233.0 35 363550.0 36 485726.0 37 547568.0 38 276657.0 39 13458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.942114634158834 18.66832954224619 14.901217296035343 23.488338527559634 2 16.235127499249366 20.114307384911836 38.61019969878174 25.04036541705706 3 19.633402707114183 23.44129709585303 29.55443591944767 27.370864277585117 4 13.639295992402007 16.52304422329832 35.51640049885937 34.321259285440306 5 14.32478166449178 37.06692908042895 33.592711202415764 15.015578052663498 6 33.66116553386781 35.37984392415144 16.49737206935941 14.46161847262134 7 29.818508116408154 32.165762566926134 19.54762772122572 18.468101595439997 8 27.589851785495295 34.232250432464305 18.993265880652807 19.18463190138759 9 26.833095745382856 16.253938581480913 19.20686904881355 37.706096624322676 10-11 25.740025449179832 26.03998588634166 26.638761758494333 21.581226905984174 12-13 26.588321399698867 23.151070079857874 27.02791183844863 23.23269668199463 14-15 23.048938899369674 24.78958325235456 25.123803359737686 27.037674488538077 16-17 22.960442284207012 27.865465860705662 26.088723807698695 23.085368047388627 18-19 23.027756359206467 26.14070087993417 28.273960451022383 22.557582309836985 20-21 23.47147483209899 25.457405768581197 28.428550809923316 22.642568589396497 22-23 23.142196313032745 25.401571846927673 28.48474631120978 22.9714855288298 24-25 23.076716790733904 25.68484059582405 28.530597978464883 22.707844634977167 26-27 22.573076171464482 26.018993785306733 28.171106490223842 23.236823553004943 28-29 22.674477992003666 26.48215257997614 28.095325046530778 22.748044381489414 30-31 22.831373421064605 25.91691321877888 27.98828240936178 23.263430950794735 32-33 22.410660452318044 25.912288012640822 28.049434564783166 23.627616970257968 34-35 23.270247029970527 26.060937906581806 27.83096954942882 22.837845514018845 36-37 23.502509514823117 25.511221178696076 27.853083477638847 23.13318582884196 38-39 22.82940090627322 25.861887649360614 27.90526618385932 23.403445260506846 40-41 23.278995387833596 25.79388564021004 28.005848224590146 22.921270747366222 42-43 23.036584770567785 25.845542217901997 27.67348508086459 23.444387930665627 44-45 22.981703615842072 26.03345124346501 27.969876193608446 23.01496894708447 46-47 23.491708225725727 26.288602015625663 27.20528171422493 23.014408044423675 48-49 23.157442920888485 26.412985409357866 27.117990364505413 23.31158130524824 50-51 23.232320036543648 26.484487781772227 27.19880341246805 23.084388769216073 52-53 23.726011827872426 26.10690825007209 27.131203086925854 23.03587683512963 54-55 23.081270144881945 26.378740804954603 27.248761665086263 23.29122738507719 56-57 23.09479924948121 26.63514596216491 26.959513024550354 23.310541763803528 58-59 23.008411848844045 26.156971963416574 27.132197430916445 23.702418756822933 60-61 22.58342018777433 26.43835624693599 27.484511585764732 23.49371197952495 62-63 22.873482382484358 26.398416763313886 27.528609235165668 23.199491619036085 64-65 22.558049350196203 26.43562933387088 27.248701401814106 23.75761991411881 66-67 22.49833861616482 26.55262741205739 27.18915851191232 23.75987545986547 68-69 23.151025462938552 26.070803873150844 27.308380125619774 23.46979053829083 70-71 22.85395105261215 26.163598384038657 27.015013856800756 23.96743670654844 72-73 22.810974303439437 26.32598031018109 27.02119248360312 23.84185290277636 74-75 22.79654124975782 26.70725096730085 26.741540769158224 23.754667013783116 76-77 22.899049075696997 26.284052138577806 27.081410558306075 23.735488227419125 78-79 22.89222426012521 26.5348528114775 26.960612829267223 23.612310099130067 80-81 23.06768643657311 26.44360894892538 26.759272581543048 23.729432032958464 82-83 22.956991507197813 26.543490827800753 26.848891819709912 23.650625845291522 84-85 22.730668169299285 26.694439254871 26.796153220314466 23.778739355515242 86-87 23.015602662069277 26.530853303397883 26.56772018773033 23.885823846802502 88-89 22.76483186823054 26.6756077409495 26.36577872618919 24.19378166463077 90-91 22.546789202030578 26.688971847380614 26.63492896683331 24.129309983755494 92-93 22.662235067118065 26.35030477667513 26.798396319809783 24.189063836397022 94-95 22.45642057534603 26.022653587114252 27.022723492573146 24.498202344966565 96-97 22.721016303926966 25.598060245795807 27.050721486960082 24.630201963317145 98-99 22.25682007805911 26.03066138719591 26.669362446956956 25.043156087788027 100-101 22.392189759401898 25.55442963675409 27.168084209485983 24.88529639435803 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 380.0 1 283.0 2 251.0 3 354.5 4 329.5 5 304.5 6 545.0 7 1219.0 8 1955.5 9 2313.5 10 2108.5 11 1786.0 12 1783.0 13 1933.0 14 2178.5 15 2440.0 16 2727.5 17 2987.0 18 3244.5 19 3668.0 20 4269.5 21 4969.0 22 5947.0 23 7201.0 24 8864.5 25 11090.0 26 14279.0 27 18561.0 28 22104.5 29 25898.0 30 30987.5 31 38604.5 32 50552.5 33 55163.0 34 54580.0 35 58821.0 36 65271.0 37 72011.5 38 77676.5 39 81652.5 40 86884.5 41 94179.0 42 98039.0 43 101016.0 44 105421.0 45 112287.5 46 116664.5 47 119263.0 48 122813.0 49 122854.0 50 120927.0 51 120260.5 52 121673.5 53 126578.0 54 131909.5 55 125563.5 56 115052.5 57 106195.0 58 99589.5 59 89696.0 60 73341.0 61 61929.0 62 53165.0 63 40286.0 64 27484.5 65 20001.0 66 15041.5 67 11853.5 68 9224.5 69 7236.5 70 5809.0 71 3995.0 72 2921.5 73 2479.5 74 1889.5 75 1352.0 76 981.0 77 666.5 78 456.5 79 282.0 80 167.0 81 102.0 82 49.5 83 27.5 84 22.0 85 13.5 86 8.5 87 7.0 88 5.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.628959937277987E-4 2 0.047457326823694676 3 0.001024475626670234 4 0.0 5 0.08702016499481284 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.807898164712178E-4 26-27 0.007969817742772852 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00986811081572064 36-37 6.02632721570726E-5 38-39 0.0037664545098170374 40-41 0.004730666864330199 42-43 3.917112690209719E-4 44-45 1.506581803926815E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.519745411780445E-5 68-69 7.834225380419438E-4 70-71 1.0546072627487704E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014613843498090106 82-83 5.875669035314579E-4 84-85 0.0045498770478589814 86-87 0.0022448068878509544 88-89 1.506581803926815E-4 90-91 0.0033144799686389927 92-93 8.888832643168209E-4 94-95 9.03949082356089E-5 96-97 0.0 98-99 1.205265443141452E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3318771.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.676343610960224 #Duplication Level Percentage of deduplicated Percentage of total 1 70.57630714179562 30.825150415177315 2 14.925552125986089 13.037870864757329 3 5.571068823830367 7.299717486897695 4 2.7197549765177143 4.75155811568027 5 1.554104937267525 3.3938810623793105 6 0.9918835085715721 2.5993106965450075 7 0.6383981020763634 1.9518026406810471 8 0.448137647789602 1.5658411111892903 9 0.34950498197036517 1.3738589717652114 >10 1.9774782748136925 15.980170822570189 >50 0.15573527020908792 4.71813736247837 >100 0.08275135876595865 6.636056138783997 >500 0.005110303186192855 1.489795389851457 >1k 0.004005372766394632 3.731320183846354 >5k 2.0717445343420505E-4 0.6455287373972763 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8730 0.2630491829656219 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7606 0.22918122401334712 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5066 0.1526468683738649 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4850 0.14613843498090107 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4736 0.14270342846794792 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4725 0.14237198047108401 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4515 0.1360443368945914 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4421 0.13321196310320899 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4151 0.1250764213620042 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3598 0.1084136266105736 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3520 0.10606335899644777 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0131636078536302E-5 2 0.0 0.0 0.0 0.0 3.0131636078536302E-5 3 0.0 0.0 0.0 0.0 3.0131636078536302E-5 4 0.0 0.0 0.0 0.0 3.0131636078536302E-5 5 0.0 0.0 0.0 0.0 6.0263272157072603E-5 6 0.0 0.0 0.0 0.0 6.0263272157072603E-5 7 0.0 0.0 0.0 0.0 6.0263272157072603E-5 8 0.0 0.0 0.0 0.0 6.0263272157072603E-5 9 0.0 0.0 0.0 0.0 6.0263272157072603E-5 10-11 0.0 0.0 0.0 0.0 6.0263272157072603E-5 12-13 0.0 0.0 0.0 0.0 2.561189066675586E-4 14-15 0.0 0.0 0.0 1.5065818039268151E-5 3.615796329424356E-4 16-17 0.0 0.0 0.0 9.03949082356089E-5 4.5197454117804455E-4 18-19 0.0 0.0 0.0 9.03949082356089E-5 4.8210617725658083E-4 20-21 0.0 0.0 0.0 1.807898164712178E-4 5.574352674529216E-4 22-23 0.0 0.0 0.0 3.615796329424356E-4 6.02632721570726E-4 24-25 0.0 7.532909019634075E-5 0.0 7.98488356081212E-4 6.327643576492623E-4 26-27 0.0 1.506581803926815E-4 0.0 0.0017777665286336417 7.231592658848712E-4 28-29 0.0 1.506581803926815E-4 0.0 0.004610140320016054 7.231592658848712E-4 30-31 0.0 1.506581803926815E-4 0.0 0.014071474048676452 7.231592658848712E-4 32-33 0.0 1.506581803926815E-4 0.0 0.03428980185737431 7.532909019634075E-4 34-35 0.0 1.506581803926815E-4 0.0 0.06405985830296818 7.532909019634075E-4 36-37 0.0 1.506581803926815E-4 0.0 0.11394278183098502 7.532909019634075E-4 38-39 0.0 1.506581803926815E-4 0.0 0.18835285712693042 8.286199921597483E-4 40-41 0.0 1.506581803926815E-4 0.0 0.2694822872683894 8.436858101990164E-4 42-43 0.0 1.506581803926815E-4 0.0 0.3555081082726106 8.436858101990164E-4 44-45 0.0 1.6572399843194964E-4 0.0 0.46113455854591956 8.436858101990164E-4 46-47 0.0 1.9585563451048597E-4 0.0 0.5864520329965521 8.587516282382846E-4 48-49 0.0 2.109214525497541E-4 0.0 0.7189709684699547 8.888832643168209E-4 50-51 0.0 2.109214525497541E-4 0.0 0.8669926307057643 9.340807184346254E-4 52-53 0.0 2.109214525497541E-4 0.0 1.0054474984866386 9.94343990591698E-4 54-55 0.0 2.109214525497541E-4 0.0 1.1558344941546133 9.94343990591698E-4 56-57 0.0 2.109214525497541E-4 0.0 1.3396826716878025 0.0010094098086309662 58-59 0.0 2.109214525497541E-4 0.0 1.5336701447614192 0.0010847388988273069 60-61 0.0 2.109214525497541E-4 0.0 1.7466104169284353 0.0011148705349058432 62-63 0.0 2.109214525497541E-4 0.0 1.9510385019032648 0.0011148705349058432 64-65 0.0 2.109214525497541E-4 0.0 2.1663290416844068 0.0011450021709843794 66-67 0.0 2.109214525497541E-4 0.0 2.4212125512727454 0.0011600679890236476 68-69 0.0 2.109214525497541E-4 0.0 2.6996288686384204 0.0011751338070629157 70-71 0.0 2.109214525497541E-4 0.0 2.9938040316731707 0.0011751338070629157 72-73 0.0 2.109214525497541E-4 0.0 3.2992634924193327 0.0012203312611807201 74-75 0.0 2.109214525497541E-4 0.0 3.605114664434515 0.0012655287152985248 76-77 0.0 2.109214525497541E-4 0.0 3.9415494470694123 0.001295660351377061 78-79 0.0 2.109214525497541E-4 0.0 4.322744775098975 0.0013257919874555973 80-81 0.0 2.109214525497541E-4 0.0 4.720407042245458 0.0013709894415734017 82-83 0.0 2.109214525497541E-4 0.0 5.140622838996725 0.0014463185317697424 84-85 0.0 2.109214525497541E-4 0.0 5.5598442917574005 0.0014463185317697424 86-87 0.0 2.109214525497541E-4 0.0 5.999525125415403 0.0014613843498090106 88-89 0.0 2.109214525497541E-4 0.0 6.4631455439378005 0.0014764501678482787 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5875 0.0 50.84272 1 GTATCAA 10275 0.0 40.18046 1 ATCAACG 13425 0.0 30.380474 3 TATCAAC 13710 0.0 29.904305 2 TCAACGC 13675 0.0 29.859833 4 CAACGCA 13935 0.0 29.268595 5 AACGCAG 14415 0.0 28.269691 6 AGGTAAG 1950 0.0 24.878805 2 TAAGGTG 2175 0.0 24.696804 5 ACGCAGA 16710 0.0 24.330204 7 TACATGG 13600 0.0 24.27082 2 CGCAGAG 16880 0.0 24.028898 8 GTACATG 14290 0.0 23.898422 1 ACATGGG 13580 0.0 23.067854 3 GGTAAGG 2240 0.0 22.919086 3 GTAAGGT 2285 0.0 22.467726 4 AAGGTAA 2115 0.0 21.589268 1 TAAGGTA 1065 0.0 20.978275 4 GCAGAGT 19000 0.0 20.94782 9 CATGGGG 7755 0.0 20.902302 4 >>END_MODULE