FastQCFastQC Report
Thu 26 May 2016
SRR938421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3320319
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91900.2767806346317929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74650.22482779516064572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49730.14977476561740002No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA49270.1483893565648361No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48780.14691359474797452No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA44990.13549902885837173No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42230.1271865745429882No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA40580.1222171725066176No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC40050.12062094033735915No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA36180.10896543374296266No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG35550.10706802569271205No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA35040.10553202869965206No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56950.052.9801331
GTATCAA107000.042.5870131
TATCAAC135650.033.0712552
ATCAACG136100.032.961913
TCAACGC138400.032.345464
CAACGCA142150.031.625895
AACGCAG146900.030.6966196
ACGCAGA168350.026.5810837
CGCAGAG170650.026.083648
TACATGG137450.025.654152
GTACATG144850.024.943291
AGGTAAG19200.024.5037442
TAAGGTG23350.024.4226535
ACATGGG141150.023.8369583
TGGTATC27300.023.5000932
GCAGAGT191200.022.5593349
GTAAGGT23450.021.8866564
GGTAAGG23000.021.6950133
GTGTAAG13300.021.503181
GTGTAGC18700.021.4111861