FastQCFastQC Report
Thu 26 May 2016
SRR938421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3320319
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90220.27172087983112464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75430.22717696703238455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50310.15152158572715452No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48140.14498606910962472No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA47330.14254654447358822No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46990.14152254647821488No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC45890.13820961178730118No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA44190.13308962181043446No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG40500.12197623180182386No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA36350.10947743274064932No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35140.10583320458064421No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA33530.10098427289667046No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61950.048.530391
GTATCAA110800.040.314741
TAAGGTG20700.032.6176765
AGGTAAG18500.031.627982
ATCAACG140500.031.405573
TATCAAC142250.031.1340372
TCAACGC143100.030.801734
CAACGCA146900.030.0696895
AACGCAG151750.029.0131766
GTAAGGT21900.028.8763684
GGTAAGG22400.026.7459223
ACGCAGA174050.025.2413147
AAGGTAA23000.025.0262371
CGCAGAG176750.024.8288618
TACATGG135700.024.2935752
GTACATG141350.023.7263721
ACATGGG135600.023.353373
GCAGAGT199850.021.6262639
AAGGTGT32200.021.387326
GAGTACT106950.020.89407212-13
Produced by FastQC (version 0.11.5)