##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938421_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3320319 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.845602786961134 31.0 31.0 34.0 30.0 34.0 2 32.079319185897496 33.0 31.0 34.0 30.0 34.0 3 32.145873333255025 33.0 31.0 34.0 30.0 34.0 4 35.808078079244794 37.0 35.0 37.0 35.0 37.0 5 35.676595531935334 37.0 35.0 37.0 33.0 37.0 6 35.7153303643415 37.0 35.0 37.0 33.0 37.0 7 35.64951289318888 37.0 35.0 37.0 33.0 37.0 8 35.632624455662246 37.0 35.0 37.0 33.0 37.0 9 37.21697764582259 39.0 37.0 39.0 33.0 39.0 10-11 37.15381744946796 39.0 37.0 39.0 33.0 39.0 12-13 37.04806074356108 39.0 37.0 39.0 33.0 39.0 14-15 38.11917017611862 40.0 37.0 41.0 33.0 41.0 16-17 38.037120228508165 40.0 37.0 41.0 33.0 41.0 18-19 38.03357900249946 40.0 37.0 41.0 33.0 41.0 20-21 38.02525540467648 40.0 37.0 41.0 33.0 41.0 22-23 38.05786100672857 40.0 37.0 41.0 33.0 41.0 24-25 37.97413486475246 40.0 37.0 41.0 33.0 41.0 26-27 37.80111263405715 40.0 37.0 41.0 32.0 41.0 28-29 37.67439589388851 40.0 37.0 41.0 32.0 41.0 30-31 37.43321439897793 39.0 36.5 41.0 32.0 41.0 32-33 37.218219695155796 39.0 36.0 41.0 31.0 41.0 34-35 37.05207481570295 39.0 36.0 41.0 31.0 41.0 36-37 37.05861695818986 39.0 36.0 41.0 31.0 41.0 38-39 36.903092745004315 39.0 36.0 41.0 31.0 41.0 40-41 36.72322162418732 39.0 35.0 41.0 30.0 41.0 42-43 36.492552221638945 39.0 35.0 40.5 30.0 41.0 44-45 36.21446448368364 39.0 35.0 40.0 30.0 41.0 46-47 35.85930388013923 38.0 35.0 40.0 28.5 41.0 48-49 35.45053502389379 38.0 34.5 40.0 27.5 41.0 50-51 35.07452356234446 38.0 34.0 40.0 26.5 41.0 52-53 34.559745464215936 38.0 33.0 40.0 25.5 41.0 54-55 34.23002789792186 37.5 33.0 40.0 24.0 41.0 56-57 33.85224235984555 37.0 33.0 40.0 23.5 41.0 58-59 33.38695769894399 36.5 32.0 39.0 22.5 41.0 60-61 32.972132195731795 36.0 31.5 39.0 22.0 40.0 62-63 32.49823857286002 35.5 31.0 39.0 20.5 40.0 64-65 32.00463871091904 35.0 31.0 38.0 19.0 40.0 66-67 31.509693195141793 35.0 30.0 38.0 17.5 40.0 68-69 30.9472668138212 34.5 29.5 37.5 13.5 39.5 70-71 30.383472341061207 34.0 29.0 36.5 9.5 39.0 72-73 29.809458669483263 34.0 28.5 36.0 7.5 39.0 74-75 29.212047547238683 34.0 28.0 35.5 6.0 38.5 76-77 27.943893192190266 31.5 26.0 34.5 2.0 36.0 78-79 28.571361215594045 33.0 27.0 35.0 2.0 37.0 80-81 28.292048595330748 33.0 27.0 35.0 2.0 36.5 82-83 27.821622410376833 33.0 26.0 35.0 2.0 36.0 84-85 27.263015089815166 33.0 26.0 35.0 2.0 36.0 86-87 26.65613981668629 32.0 25.0 35.0 2.0 35.0 88-89 25.922742664183772 32.0 23.5 34.0 2.0 35.0 90-91 25.402625771800842 31.5 21.0 34.0 2.0 35.0 92-93 24.665057483934525 31.0 18.5 34.0 2.0 35.0 94-95 23.90969226149656 31.0 11.0 34.0 2.0 35.0 96-97 22.95831138514101 30.5 2.0 34.0 2.0 35.0 98-99 21.807350287728376 30.0 2.0 34.0 2.0 35.0 100-101 18.622992700400175 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29585337098806974 1101 2 0.2385622268089591 1101 3 0.2557297877502194 1101 4 0.27345640791238424 1101 5 0.19053212436190137 1101 6 0.16630953783035807 1101 7 0.2485516799454004 1101 8 0.3212634464513826 1101 9 0.37829293056950775 1101 10-11 0.35113781373016906 1101 12-13 0.40249157550712056 1101 14-15 0.5242439559686858 1101 16-17 0.5528377108286548 1101 18-19 0.5996360396250893 1101 20-21 0.5929639662197488 1101 22-23 0.6333867680782674 1101 24-25 0.5572619225468074 1101 26-27 0.5397458535202588 1101 28-29 0.5537853733498324 1101 30-31 0.6237407297079613 1101 32-33 0.5749607130427279 1101 34-35 0.42044086120575486 1101 36-37 0.6700984049996421 1101 38-39 0.7956572998527491 1101 40-41 0.8865057603540905 1101 42-43 0.712879574991689 1101 44-45 0.8167408925273278 1101 46-47 0.9802317375209668 1101 48-49 0.8891523960543495 1101 50-51 0.9596860271468657 1101 52-53 0.9646174204849132 1101 54-55 0.9640566815838483 1101 56-57 1.113528907898889 1101 58-59 1.2025723232237127 1101 60-61 1.237392333471071 1101 62-63 1.3144854170272353 1101 64-65 1.302588253109768 1101 66-67 1.3231484997339429 1101 68-69 1.4173680948357266 1101 70-71 1.4674571822097917 1101 72-73 1.4959129101998627 1101 74-75 1.6365206380069353 1101 76-77 1.5276039493667355 1101 78-79 1.7755312036922533 1101 80-81 2.145522142636093 1101 82-83 2.2585960028724266 1101 84-85 2.1896237969856287 1101 86-87 2.191552145316791 1101 88-89 2.5266227049012393 1101 90-91 2.7017322309977487 1101 92-93 3.089923523225238 1101 94-95 3.137059862779658 1101 96-97 3.5008242489754196 1101 98-99 3.7734855309241944 1101 100-101 4.088989581145459 1102 1 0.35977293398990895 1102 2 0.3392589031729756 1102 3 0.3009010733381956 1102 4 0.21229711347448443 1102 5 0.13452446684265595 1102 6 0.2017883289942901 1102 7 0.28642321638551493 1102 8 0.25293142387079115 1102 9 0.3792596441815448 1102 10-11 0.41883633769329975 1102 12-13 0.42008019818864284 1102 14-15 0.5794912659804865 1102 16-17 0.4869648126132091 1102 18-19 0.5025793125261018 1102 20-21 0.5227268580485713 1102 22-23 0.5037176560755796 1102 24-25 0.46797200783102966 1102 26-27 0.44467183595946125 1102 28-29 0.447725358391871 1102 30-31 0.46939305428415423 1102 32-33 0.3586051005846258 1102 34-35 0.345477240618294 1102 36-37 0.5762597728517918 1102 38-39 0.5844983037478357 1102 40-41 0.611676424032531 1102 42-43 0.6275545056350111 1102 44-45 0.6285806730276917 1102 46-47 0.609937969031499 1102 48-49 0.8148866451628507 1102 50-51 0.8162567078532348 1102 52-53 0.9629420512921101 1102 54-55 0.7657393140373827 1102 56-57 0.9701637875685734 1102 58-59 1.0554402899949267 1102 60-61 1.1184947059755999 1102 62-63 1.189870926351638 1102 64-65 1.1369254386868874 1102 66-67 1.05617455774318 1102 68-69 1.0892683061243282 1102 70-71 1.3495941390802493 1102 72-73 1.32445216634558 1102 74-75 1.2792298273742553 1102 76-77 1.3419393426510773 1102 78-79 1.767460780125969 1102 80-81 1.813440813392834 1102 82-83 1.789440592729978 1102 84-85 1.8731502741527137 1102 86-87 2.020283309891724 1102 88-89 2.3041992290328217 1102 90-91 2.368083276666333 1102 92-93 2.62152255130227 1102 94-95 2.843615064593891 1102 96-97 3.2919838738744787 1102 98-99 3.5607315434170026 1102 100-101 4.171417607739318 1103 1 0.3089720638378921 1103 2 0.3306114168301235 1103 3 0.3591843854692982 1103 4 0.25566348720214904 1103 5 0.15461147987047497 1103 6 0.1392491693809248 1103 7 0.1764447027346776 1103 8 0.15997184262574393 1103 9 0.30652221502219135 1103 10-11 0.36791364669304727 1103 12-13 0.37367623982103737 1103 14-15 0.5247297233599895 1103 16-17 0.6263500418815653 1103 18-19 0.542370970064816 1103 20-21 0.4964905286471719 1103 22-23 0.47670392375114545 1103 24-25 0.43869608246823333 1103 26-27 0.404452201830793 1103 28-29 0.362410973083783 1103 30-31 0.3964317161226347 1103 32-33 0.4214082325491546 1103 34-35 0.5150120698022036 1103 36-37 0.5653010607150506 1103 38-39 0.6106833716321063 1103 40-41 0.5902096988454772 1103 42-43 0.6224343878868197 1103 44-45 0.6973969494671906 1103 46-47 0.7990741042914493 1103 48-49 0.815428288595129 1103 50-51 0.7917247287661908 1103 52-53 0.9696065921188364 1103 54-55 0.9681556069153601 1103 56-57 0.9735168778770245 1103 58-59 1.0649870544690785 1103 60-61 1.1526367789826608 1103 62-63 1.2681835211487495 1103 64-65 1.127186674553684 1103 66-67 1.2819365305690376 1103 68-69 1.3874576928050182 1103 70-71 1.4176576376945569 1103 72-73 1.5010440702005 1103 74-75 1.4735880054562216 1103 76-77 1.3283440055247873 1103 78-79 1.5153728925538381 1103 80-81 1.7666123574777899 1103 82-83 1.9750502835159374 1103 84-85 2.0448858151704634 1103 86-87 2.2798620652490413 1103 88-89 2.366015237042447 1103 90-91 2.374561525638139 1103 92-93 2.548642046519298 1103 94-95 2.703048156789716 1103 96-97 3.017865601867058 1103 98-99 3.385705371776048 1103 100-101 3.990437761506474 1104 1 0.28760932981992227 1104 2 0.2451078451027655 1104 3 0.24832862795069843 1104 4 0.19595748784390565 1104 5 0.1093265126033387 1104 6 0.1696789896617048 1104 7 0.18156121935869862 1104 8 0.23821862236289348 1104 9 0.2260572922399362 1104 10-11 0.31675473675369403 1104 12-13 0.343699044813377 1104 14-15 0.4479132063947091 1104 16-17 0.38605322848965073 1104 18-19 0.5015640147954983 1104 20-21 0.5567845685847104 1104 22-23 0.467682544678361 1104 24-25 0.462265274208967 1104 26-27 0.47632489675877565 1104 28-29 0.46616008983369994 1104 30-31 0.4258493082823378 1104 32-33 0.455828331882671 1104 34-35 0.5839818928192031 1104 36-37 0.5961894523400417 1104 38-39 0.6266182217202783 1104 40-41 0.5549418448880132 1104 42-43 0.6229215520772797 1104 44-45 0.6417062026603091 1104 46-47 0.5868012791083217 1104 48-49 0.6989458415521312 1104 50-51 0.630850207515401 1104 52-53 0.8574672588171453 1104 54-55 0.9364257686722794 1104 56-57 0.9816315069595518 1104 58-59 1.0562430208343798 1104 60-61 1.031784176501084 1104 62-63 1.0502207712676963 1104 64-65 1.0787231962013593 1104 66-67 1.3175803313349057 1104 68-69 1.4126587966853528 1104 70-71 1.4053422386423513 1104 72-73 1.5239658640608695 1104 74-75 1.7181948931161166 1104 76-77 1.5893539317407708 1104 78-79 1.8851701191201364 1104 80-81 2.1023536944916863 1104 82-83 2.220159226396536 1104 84-85 2.2863785127229868 1104 86-87 2.3252483225447413 1104 88-89 2.405586391917332 1104 90-91 2.6173733719904178 1104 92-93 2.8967283578260066 1104 94-95 3.0529102234516863 1104 96-97 3.4880436768725573 1104 98-99 3.835549124539032 1104 100-101 4.217120864147763 1105 1 0.24034915464947915 1105 2 0.22823823114703146 1105 3 0.23314505257170026 1105 4 0.08188202360532415 1105 5 0.06540198752719562 1105 6 0.12531403715529876 1105 7 0.15945766840039965 1105 8 0.16195551408564768 1105 9 0.19282240910523285 1105 10-11 0.24541569262886753 1105 12-13 0.2890272744728719 1105 14-15 0.38607773916425003 1105 16-17 0.3965204225229968 1105 18-19 0.379432342147517 1105 20-21 0.3810811742141951 1105 22-23 0.269741450632921 1105 24-25 0.32513828122384325 1105 26-27 0.4233086148347027 1105 28-29 0.5069031241300195 1105 30-31 0.4306467034076178 1105 32-33 0.39337753876846193 1105 34-35 0.4636478712573009 1105 36-37 0.630483002953504 1105 38-39 0.6112497425888535 1105 40-41 0.4848408836507687 1105 42-43 0.630184427707043 1105 44-45 0.6448081853122787 1105 46-47 0.6829422935970655 1105 48-49 0.8284975118785027 1105 50-51 0.7776893344510754 1105 52-53 0.8528577301784352 1105 54-55 0.8387537491706851 1105 56-57 0.8633559542275009 1105 58-59 1.1192879635686097 1105 60-61 1.165854647804089 1105 62-63 1.2180897251651217 1105 64-65 1.268660876788509 1105 66-67 1.291147011552443 1105 68-69 1.3933015545731529 1105 70-71 1.5087811473070865 1105 72-73 1.583251194301301 1105 74-75 1.6642522467922625 1105 76-77 1.5268444182071441 1105 78-79 1.8836131780411485 1105 80-81 2.1379265148543176 1105 82-83 2.179349020603077 1105 84-85 2.4152709504169287 1105 86-87 2.648281054928738 1105 88-89 2.7212060720488935 1105 90-91 2.854112003955212 1105 92-93 3.0039046827631424 1105 94-95 3.244833081625604 1105 96-97 3.5272297215204773 1105 98-99 3.8262472564137298 1105 100-101 4.419654973231747 1106 1 0.15495676449751983 1106 2 0.20480649596031952 1106 3 0.16664534707046386 1106 4 0.1958770760261075 1106 5 0.1531503677839936 1106 6 0.17568557679483376 1106 7 0.172466974561317 1106 8 0.1253754693218383 1106 9 0.15075055140702887 1106 10-11 0.2068469566175608 1106 12-13 0.16665008045708873 1106 14-15 0.17989232274300093 1106 16-17 0.14934996663890843 1106 18-19 0.35968019230653425 1106 20-21 0.434884449040446 1106 22-23 0.348563219981493 1106 24-25 0.32032899862283415 1106 26-27 0.3297204419050317 1106 28-29 0.32937031696781105 1106 30-31 0.35830084406023843 1106 32-33 0.27043278673641424 1106 34-35 0.29071160078963487 1106 36-37 0.4042565923044279 1106 38-39 0.436223237700176 1106 40-41 0.38951375442471914 1106 42-43 0.4941198735781711 1106 44-45 0.13476365709930604 1106 46-47 -0.06576921541460479 1106 48-49 0.4904023298782647 1106 50-51 0.2963850583290366 1106 52-53 0.5767451138514232 1106 54-55 0.6030515463199393 1106 56-57 0.5963527736408594 1106 58-59 0.8211541433306522 1106 60-61 0.6280542001659697 1106 62-63 0.8352934005096913 1106 64-65 0.5141083971089202 1106 66-67 0.7204908683082429 1106 68-69 0.6592287544789137 1106 70-71 0.8394716880068174 1106 72-73 1.178175920417452 1106 74-75 1.4575770200282143 1106 76-77 1.55493064744498 1106 78-79 1.6838044844376618 1106 80-81 1.57522207381297 1106 82-83 1.8286803906113214 1106 84-85 1.7599056541511757 1106 86-87 2.080256788230656 1106 88-89 2.4742603776208085 1106 90-91 2.403977241321215 1106 92-93 2.0000354402139635 1106 94-95 2.543109203665889 1106 96-97 2.977054435856676 1106 98-99 3.4533418494164394 1106 100-101 4.054689724085794 1107 1 0.17776889554163233 1107 2 0.17224951339981942 1107 3 0.16830385727730146 1107 4 0.08805065991145966 1107 5 0.047804777229266904 1107 6 0.07545232908179855 1107 7 0.10000138040152251 1107 8 0.12758501373173203 1107 9 0.12010803867664777 1107 10-11 0.20437703637962557 1107 12-13 0.19972159593633876 1107 14-15 0.17270706745932785 1107 16-17 0.24317719389320303 1107 18-19 0.25490546381301016 1107 20-21 0.28949289390547506 1107 22-23 0.26392341104994443 1107 24-25 0.17242191917620886 1107 26-27 0.2902891409271575 1107 28-29 0.19949757576642213 1107 30-31 0.14787932182149177 1107 32-33 0.2868553003680674 1107 34-35 0.33167929039353083 1107 36-37 0.3945650056251395 1107 38-39 0.4017903595063146 1107 40-41 0.4446761175448941 1107 42-43 0.4797013255181426 1107 44-45 0.4039873063264636 1107 46-47 0.4551166392111483 1107 48-49 0.4106317021462971 1107 50-51 0.36198138116261447 1107 52-53 0.47913256822482 1107 54-55 0.3396088893869518 1107 56-57 0.43829815690434515 1107 58-59 0.4918382945631308 1107 60-61 0.5755474181372264 1107 62-63 0.7049501081839722 1107 64-65 0.8948381524151259 1107 66-67 0.9716242106542374 1107 68-69 1.1054428052202034 1107 70-71 1.0925559113406003 1107 72-73 1.2449032423047015 1107 74-75 1.176368602201169 1107 76-77 1.2627015455568298 1107 78-79 1.3444481688142105 1107 80-81 1.5731564616278497 1107 82-83 1.7262985240888575 1107 84-85 1.885133178823338 1107 86-87 2.1426132390440493 1107 88-89 2.48260916767083 1107 90-91 2.608706005234513 1107 92-93 2.8493876100125277 1107 94-95 3.03371782878418 1107 96-97 3.1172293716414536 1107 98-99 3.4340766518138075 1107 100-101 3.702166629137391 1108 1 0.18364057423153923 1108 2 0.1782849199753258 1108 3 0.1315150082557608 1108 4 0.09916633769699956 1108 5 0.09380769037532133 1108 6 0.12004094595938852 1108 7 0.15799130962367514 1108 8 0.16606325212726603 1108 9 0.1773537960820093 1108 10-11 0.16414637169315682 1108 12-13 0.23139348626219203 1108 14-15 0.2829705701770564 1108 16-17 0.2921565001341122 1108 18-19 0.30856265561080676 1108 20-21 0.2327915233148019 1108 22-23 0.3010649306094564 1108 24-25 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-0.14715411056612737 2316 56-57 -0.10905827579080807 2316 58-59 0.016455755807520234 2316 60-61 0.2196576894601847 2316 62-63 0.3557866598276931 2316 64-65 0.3594723939368407 2316 66-67 0.35852946201693925 2316 68-69 0.5931351779523446 2316 70-71 0.47629325818920876 2316 72-73 0.6046226558126406 2316 74-75 0.8202791083179335 2316 76-77 0.7904770391975582 2316 78-79 0.8789332182468073 2316 80-81 1.0017898771436613 2316 82-83 1.15897962402396 2316 84-85 1.2464803303340979 2316 86-87 1.4579004572763878 2316 88-89 1.7034789871899392 2316 90-91 1.9253407094681876 2316 92-93 1.7417383215250908 2316 94-95 2.362379095286297 2316 96-97 2.4810687102420843 2316 98-99 2.9656648734714217 2316 100-101 3.4272326794122527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 11.0 4 38.0 5 124.0 6 198.0 7 663.0 8 1466.0 9 2597.0 10 4150.0 11 6451.0 12 9277.0 13 12693.0 14 16693.0 15 22360.0 16 27589.0 17 34806.0 18 33867.0 19 30117.0 20 30200.0 21 32072.0 22 35149.0 23 39581.0 24 45545.0 25 53161.0 26 62557.0 27 75086.0 28 89273.0 29 106376.0 30 125963.0 31 149320.0 32 179877.0 33 219452.0 34 276460.0 35 355077.0 36 454575.0 37 501602.0 38 269391.0 39 16496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.972075875842044 18.64944302038449 14.903929411601716 23.474551692171747 2 16.27826323812244 20.086487971862482 38.5321631665011 25.103085623513977 3 19.648882817177643 23.38882805130596 29.556463902833347 27.40582522868305 4 13.650375159736159 16.55292759520998 35.4198497192589 34.376847525794965 5 14.317711689053292 37.05568069339394 33.56643040388183 15.060177213670942 6 33.69968367497219 35.3545849058479 16.482753614938805 14.462977804241097 7 29.825477612241475 32.15660904870887 19.571613450394374 18.446299888655275 8 27.6008419672929 34.19831046354281 18.998204690573406 19.202642878590883 9 26.812995980205518 16.259311228830725 19.226706831482158 37.700985959481606 10-11 25.736427734805 26.010994726711502 26.625348347553352 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23.52132225590321 25.490854554290255 27.848721366409812 23.13910182339672 38-39 22.84872046231645 25.88499530330895 27.858674856896638 23.40760937747796 40-41 23.305375095894036 25.770066363038623 28.027638565560796 22.896919975506545 42-43 23.034241721185097 25.818634867118195 27.677928973284487 23.46919443841222 44-45 23.033520217695433 26.02110995155463 27.959316132743616 22.986053698006316 46-47 23.485713795227777 26.340093578372077 27.190132512889953 22.984060113510193 48-49 23.18519696450853 26.38884998700426 27.081449101727877 23.344503946759332 50-51 23.243640144215057 26.510253984632197 27.166169877050972 23.07993599410177 52-53 23.716200160285805 26.09195080352219 27.141548748779858 23.050300287412142 54-55 23.11000840581884 26.380612826659128 27.218182951698317 23.29119581582372 56-57 23.137400352195076 26.619159183198963 26.938736910519744 23.30470355408622 58-59 23.04344853611957 26.140334708803582 27.11316292199635 23.703053833080496 60-61 22.6159896082274 26.405444778046927 27.49530993859325 23.48325567513242 62-63 22.86573970754015 26.38046223871863 27.512778139690795 23.241019914050426 64-65 22.57412616076949 26.435140719912752 27.21607472053137 23.774658398786382 66-67 22.501175341707448 26.58909060024799 27.148375558795546 23.761358499249017 68-69 23.174632862007076 26.046020079742515 27.313807755687986 23.465539302562423 70-71 22.902543400197388 26.133061311277622 26.993129274626927 23.971266013898063 72-73 22.798082652662778 26.334296745506037 27.005678524923756 23.86194207690743 74-75 22.854159494916 26.707448892711817 26.6754188377683 23.762972774603885 76-77 22.857803723076007 26.285275601531055 27.082021335901764 23.774899339491178 78-79 22.95368306478986 26.546967324525145 26.92726210945394 23.572087501231056 80-81 23.088195166848475 26.44745171998586 26.7802059923176 23.684147120848063 82-83 23.024224874638037 26.53502236479363 26.80246924549109 23.638283515077244 84-85 22.74913562525102 26.690073066942716 26.78505301453282 23.77573829327344 86-87 23.03273994310405 26.48539434199884 26.594421570645654 23.887444144251457 88-89 22.78242626158097 26.672776523212537 26.303534781909445 24.241262433297052 90-91 22.59232409728436 26.6957043171268 26.626553668402764 24.085417917186074 92-93 22.723002856665275 26.336691960527723 26.778684488322607 24.16162069448439 94-95 22.480701402485725 25.98903900498717 27.028186147174416 24.50207344535269 96-97 22.788111624214423 25.57385299424543 27.026183327565818 24.61185205397433 98-99 22.31499443276384 25.99909827941231 26.643599605941475 25.042307681882374 100-101 22.447466632854404 25.51348984254258 27.149007414854314 24.8900361097487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 374.0 1 280.0 2 263.0 3 363.5 4 338.0 5 332.5 6 595.5 7 1297.5 8 2070.0 9 2415.0 10 2157.5 11 1795.5 12 1879.0 13 2084.0 14 2195.5 15 2381.0 16 2668.5 17 2930.0 18 3244.5 19 3690.0 20 4216.0 21 4938.0 22 5915.5 23 7270.5 24 8948.0 25 11234.0 26 14580.5 27 18710.0 28 22327.5 29 26167.0 30 31093.5 31 38685.5 32 50408.5 33 54829.5 34 54328.0 35 58382.5 36 64913.0 37 71821.5 38 77330.5 39 81312.0 40 86825.5 41 94190.0 42 97756.5 43 100208.0 44 104649.0 45 112055.0 46 116313.0 47 119082.0 48 122298.5 49 122045.0 50 120674.0 51 120183.5 52 121705.5 53 126678.5 54 132076.0 55 125877.5 56 115372.0 57 106478.5 58 99554.0 59 89953.5 60 74012.0 61 62157.0 62 53223.0 63 40740.0 64 28335.5 65 20702.5 66 15555.5 67 12233.5 68 9484.0 69 7489.0 70 5973.0 71 4082.0 72 3006.0 73 2550.0 74 1900.5 75 1317.0 76 1000.0 77 730.5 78 491.0 79 316.0 80 194.0 81 109.0 82 62.0 83 37.5 84 24.0 85 13.5 86 10.5 87 8.5 88 3.0 89 2.0 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.04445356003444247 3 9.03527642976473E-5 4 0.0 5 0.10637532116643009 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012348211120678464 26-27 0.012047035239686308 28-29 0.0 30-31 0.0 32-33 4.969402036370602E-4 34-35 0.015661145811592198 36-37 0.0034484638373602056 38-39 0.012905386500513958 40-41 0.00746916184860551 42-43 0.005662106562652564 44-45 0.0025298774003341246 46-47 1.5058794049607885E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.4094070479372615E-4 68-69 2.2588191074411824E-4 70-71 0.0 72-73 7.529397024803942E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.011758809921577E-5 82-83 0.0010089392013237282 84-85 0.004351991480336679 86-87 0.0014305854347127491 88-89 3.011758809921577E-5 90-91 9.637628191749046E-4 92-93 4.2164623338902075E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.378809084307863E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3320319.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.71164733203283 #Duplication Level Percentage of deduplicated Percentage of total 1 70.71838677552272 30.91217182621943 2 14.841915488089864 12.9752915109444 3 5.481051480207668 7.187573679346623 4 2.7340955327368506 4.780472787963185 5 1.5024639563209696 3.2837587293896506 6 0.9973198651359992 2.6156696533233204 7 0.6619774334961468 2.0255286880323453 8 0.46844841711481167 1.6381321601737326 9 0.36164911690453555 1.4227450790464955 >10 1.980835744617734 15.8284227953543 >50 0.15850832312104104 4.749762723524913 >100 0.08375302108005106 6.650816653551145 >500 0.0053151303258477944 1.528812159479775 >1k 0.004072632326320468 3.7492226346524493 >5k 2.0708299964341357E-4 0.6516189189981594 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9022 0.27172087983112464 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7543 0.22717696703238455 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5031 0.15152158572715452 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4814 0.14498606910962472 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4733 0.14254654447358822 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4699 0.14152254647821488 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4589 0.13820961178730118 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4419 0.13308962181043446 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4050 0.12197623180182386 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3635 0.10947743274064932 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3514 0.10583320458064421 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3353 0.10098427289667046 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.03527642976473E-5 2 0.0 0.0 0.0 0.0 9.03527642976473E-5 3 0.0 0.0 0.0 0.0 9.03527642976473E-5 4 0.0 0.0 0.0 0.0 9.03527642976473E-5 5 0.0 3.011758809921577E-5 0.0 0.0 9.03527642976473E-5 6 0.0 3.011758809921577E-5 0.0 0.0 9.03527642976473E-5 7 0.0 3.011758809921577E-5 0.0 0.0 9.03527642976473E-5 8 0.0 3.011758809921577E-5 0.0 0.0 9.03527642976473E-5 9 0.0 3.011758809921577E-5 0.0 0.0 9.03527642976473E-5 10-11 0.0 6.023517619843154E-5 0.0 1.5058794049607885E-5 9.03527642976473E-5 12-13 0.0 9.03527642976473E-5 0.0 3.011758809921577E-5 2.2588191074411824E-4 14-15 0.0 9.03527642976473E-5 0.0 3.011758809921577E-5 3.011758809921577E-4 16-17 0.0 9.03527642976473E-5 0.0 3.011758809921577E-5 3.3129346909137347E-4 18-19 0.0 9.03527642976473E-5 0.0 3.011758809921577E-5 3.3129346909137347E-4 20-21 0.0 9.03527642976473E-5 0.0 9.035276429764731E-5 3.9152864528980497E-4 22-23 0.0 9.03527642976473E-5 0.0 1.6564673454568673E-4 4.2164623338902075E-4 24-25 0.0 9.03527642976473E-5 0.0 4.668226155378444E-4 4.2164623338902075E-4 26-27 0.0 9.03527642976473E-5 0.0 0.0012047035239686307 4.2164623338902075E-4 28-29 0.0 9.03527642976473E-5 0.0 0.004186344745790991 4.2164623338902075E-4 30-31 0.0 9.03527642976473E-5 0.0 0.013703502585143173 4.3670502743862864E-4 32-33 0.0 9.03527642976473E-5 0.0 0.03279805344004597 5.27057791736276E-4 34-35 0.0 9.03527642976473E-5 0.0 0.06308128827380743 5.722341738850996E-4 36-37 0.0 9.03527642976473E-5 0.0 0.11197719255288421 5.722341738850996E-4 38-39 0.0 1.0541155834725519E-4 0.0 0.18614175324720306 5.722341738850996E-4 40-41 0.0 1.5058794049607884E-4 0.0 0.2681067692592188 5.722341738850996E-4 42-43 0.0 1.5058794049607884E-4 0.0 0.3525263687013206 5.722341738850996E-4 44-45 0.0 1.5058794049607884E-4 0.0 0.4601515697738681 5.722341738850996E-4 46-47 0.0 1.8070552859529462E-4 0.0 0.58527508953206 5.722341738850996E-4 48-49 0.0 2.1082311669451038E-4 0.0 0.7180183590793535 5.872929679347075E-4 50-51 0.0 2.1082311669451038E-4 0.0 0.8669498322299756 6.324693500835312E-4 52-53 0.0 2.1082311669451038E-4 0.0 1.0064243827174437 6.324693500835312E-4 54-55 0.0 2.1082311669451038E-4 0.0 1.1570424408016216 6.625869381827469E-4 56-57 0.0 2.1082311669451038E-4 0.0 1.3328990377129428 6.776457322323548E-4 58-59 0.0 2.2588191074411827E-4 0.0 1.5300638282044585 6.927045262819627E-4 60-61 0.0 2.4094070479372615E-4 0.0 1.7470008152831098 8.131748786788257E-4 62-63 0.0 2.4094070479372615E-4 0.0 1.9535171168794323 8.432924667780415E-4 64-65 0.0 2.4094070479372615E-4 0.0 2.1685717547018823 8.432924667780415E-4 66-67 0.0 2.4094070479372615E-4 0.0 2.4204752615637233 8.432924667780415E-4 68-69 0.0 2.4094070479372615E-4 0.0 2.7000116555065947 8.432924667780415E-4 70-71 0.0 2.4094070479372615E-4 0.0 2.9910228505152667 9.18586437026081E-4 72-73 0.0 2.4094070479372615E-4 0.0 3.297168133543795 0.0010089392013237282 74-75 0.0 2.4094070479372615E-4 0.0 3.601732243197114 0.0010691743775221598 76-77 0.0 2.4094070479372615E-4 0.0 3.937091586681882 0.0011143507596709835 78-79 0.0 2.4094070479372615E-4 0.0 4.31737131281663 0.0011143507596709835 80-81 0.0 2.4094070479372615E-4 0.0 4.725931454176542 0.0011143507596709835 82-83 0.0 2.4094070479372615E-4 0.0 5.144550869961591 0.0011745859358694149 84-85 0.0 2.4094070479372615E-4 0.0 5.560836172668951 0.0011745859358694149 86-87 0.0 2.4094070479372615E-4 0.0 5.998264022221961 0.0012047035239686307 88-89 0.0 2.4094070479372615E-4 0.0 6.461291821659304 0.0012348211120678464 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6195 0.0 48.53039 1 GTATCAA 11080 0.0 40.31474 1 TAAGGTG 2070 0.0 32.617676 5 AGGTAAG 1850 0.0 31.62798 2 ATCAACG 14050 0.0 31.40557 3 TATCAAC 14225 0.0 31.134037 2 TCAACGC 14310 0.0 30.80173 4 CAACGCA 14690 0.0 30.069689 5 AACGCAG 15175 0.0 29.013176 6 GTAAGGT 2190 0.0 28.876368 4 GGTAAGG 2240 0.0 26.745922 3 ACGCAGA 17405 0.0 25.241314 7 AAGGTAA 2300 0.0 25.026237 1 CGCAGAG 17675 0.0 24.828861 8 TACATGG 13570 0.0 24.293575 2 GTACATG 14135 0.0 23.726372 1 ACATGGG 13560 0.0 23.35337 3 GCAGAGT 19985 0.0 21.626263 9 AAGGTGT 3220 0.0 21.38732 6 GAGTACT 10695 0.0 20.894072 12-13 >>END_MODULE