FastQCFastQC Report
Thu 26 May 2016
SRR938420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306310
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86570.2618326775166273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70410.2129564378415817No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48180.1457213630905753No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47950.14502572354074483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45290.13698050092096628No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA44970.13601265459076736No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA40400.12219059918761399No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC40390.12216035398979527No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39350.11901485341664877No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG35410.10709824547607454No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA34920.10561623078295744No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA34850.10540451439822643No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57200.053.8729741
GTATCAA104950.043.133851
ATCAACG135600.033.225353
TATCAAC137300.033.0562632
TCAACGC138250.032.4853554
CAACGCA141050.031.705715
AACGCAG145300.030.837676
ACGCAGA167300.026.7488427
CGCAGAG167250.026.5864168
TGGTATC26700.025.2753982
TACATGG136000.024.1817722
GCAGAGT184600.023.4428529
GTACATG143000.023.3505151
ACATGGG137700.022.6752643
GTGGTAT31450.022.5995661
AGGTAAG18250.022.395282
TAAGGTG21200.022.1932165
GAGTACT99200.021.64448412-13
CAGAGTA171500.021.24443210-11
AGAGTAC162150.021.1511610-11
Produced by FastQC (version 0.11.5)